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Protein

UDP-galactose:fucoside alpha-3-galactosyltransferase

Gene

agtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the transfer of a galactosyl residue to the hydroxyproline-linked saccharide on Skp1 protein (fpaA/fpaB). Catalyzes the formation of a Gal-alpha-1,3-Fuc linkage, leading to Gal-Fuc-Gal-GlcNAc-HyPro143-Skp1.2 Publications

Catalytic activityi

UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.1 Publication

Cofactori

Mn2+1 PublicationNote: Divalent metal cations. Mn(2+) is 4-fold better than Mg2+.1 Publication

Enzyme regulationi

Stimulated by dithiothreitol (DTT) in vitro. Totally inhibited by EDTA.1 Publication

Kineticsi

The catalytic efficiency with the presumed natural substrate Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 is 21-fold and 21000-fold higher than that of the synthetic substrates Fuc-alpha-1,2-Gal-beta-1-pNP and Fuc-alpha-1-Bn, respectively.

  1. KM=3.5 µM for UDP-Gal1 Publication
  2. KM=3.8 mM for Fuc-alpha-1-Bn1 Publication
  3. KM=0.84 mM for Fuc-alpha-1,2-Gal-beta-1-Bn1 Publication
  4. KM=0.82 mM for Fuc-alpha-1,2-Gal-beta-1-pNP1 Publication
  5. KM=0.006 mM for Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp11 Publication

    pH dependencei

    Optimum pH is 6.4-7.4.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Pathway:iprotein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    • protein glycosylation Source: dictyBase
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Manganese

    Enzyme and pathway databases

    SABIO-RKQ54RP0.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT77. Glycosyltransferase Family 77.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-galactose:fucoside alpha-3-galactosyltransferase (EC:2.4.1.37)
    Alternative name(s):
    Alpha-GalT1
    Fucosylgalactoside 3-alpha-galactosyltransferase
    Skp1 alpha-3-galactosyltransferase
    Gene namesi
    Name:agtA
    Synonyms:GT78
    ORF Names:DDB_G0283005
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283005. agtA.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: dictyBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells show an accumulation of the non-galactosylated isoform of Skp1 in cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648UDP-galactose:fucoside alpha-3-galactosyltransferasePRO_0000328554Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi1248885. 1 interaction.
    STRINGi44689.DDB0231341.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54RP0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati320 – 35839WD 1Add
    BLAST
    Repeati372 – 42049WD 2Add
    BLAST
    Repeati422 – 46140WD 3Add
    BLAST
    Repeati464 – 50542WD 4Add
    BLAST
    Repeati507 – 54640WD 5Add
    BLAST
    Repeati556 – 59540WD 6Add
    BLAST
    Repeati617 – 64832WD 7Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi168 – 1714Poly-Asp
    Compositional biasi386 – 3916Poly-Asn
    Compositional biasi590 – 60415Poly-AsnAdd
    BLAST

    Domaini

    Consists of two domains: an N-terminal catalytic domain and a C-terminal domain, not required for activity, that is predicted to form a beta-propeller-like protein-protein interaction domain.1 Publication

    Sequence similaritiesi

    Belongs to the glycosyltransferase 77 family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    InParanoidiQ54RP0.
    OMAiSSINCIH.
    PhylomeDBiQ54RP0.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR005069. Nucl-diP-sugar_transferase.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03407. Nucleotid_trans. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54RP0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKKVEYIKE NDKIVLMCNY GFRDMTLNLL KCFEKLSIDK SRYILYCMDD
    60 70 80 90 100
    KAYQFFAEFK GIECQRFSRD DIINSSTSST QLFHDNNTND NKGIYSENAE
    110 120 130 140 150
    SYGDIGFRAI CNEKPLVVLD VLKKGYNVLW TDTDIVWKRD PFIHFYQDIN
    160 170 180 190 200
    QENQFTNDDD IDLYVQQDDD DICAGFYFIR SNQRTIKFIQ DSINFLNPCI
    210 220 230 240 250
    DDQIAMRLFL KSQGINIKSK NILLSLSEND KKDKIRYRLL DKKLFPNGTN
    260 270 280 290 300
    YFNLKITQRD NITPFIIHNN CIIGHRSKKD RFIEYGLWYI NDDEIDINSN
    310 320 330 340 350
    INNDDENNKE IKLFKNVLKN HTDIITSINS SDDGKLFTTS IDKSIKIWKF
    360 370 380 390 400
    ENTSGNDTDN GIFKVLKSKY IHKRGGIWST FIFSSNNNNN NDYGFENLLT
    410 420 430 440 450
    SSHDKTIQYW DNNLQVIQTF IGHTGIINQL IVIPNSSYFF TCSDDNTIRQ
    460 470 480 490 500
    FDLNNINFKR VFIGHNGWVS SIAINKNLNT LYSCSNDGTI RFWDINSGRC
    510 520 530 540 550
    LNIIKGNQGG WIRKIIYNDN LNQLISGGND GTIKIWSCDN LNNFNDNQYL
    560 570 580 590 600
    LKINTNENSS INDLQFDSDT NLIYCAFENG SLKSFKLTSN NNNNNNNNNN
    610 620 630 640
    NNNNTINYSL DKVYQGHLNS SINCIHISKS LNLLFSGGFD KQIKSWDL
    Length:648
    Mass (Da):75,244
    Last modified:May 24, 2005 - v1
    Checksum:i1A92113B7C48E9D4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ340632 Genomic DNA. Translation: ABC67744.1.
    AAFI02000049 Genomic DNA. Translation: EAL65946.1.
    RefSeqiXP_639311.1. XM_634219.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231341; DDB0231341; DDB_G0283005.
    GeneIDi8623882.
    KEGGiddi:DDB_G0283005.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ340632 Genomic DNA. Translation: ABC67744.1.
    AAFI02000049 Genomic DNA. Translation: EAL65946.1.
    RefSeqiXP_639311.1. XM_634219.1.

    3D structure databases

    ProteinModelPortaliQ54RP0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi1248885. 1 interaction.
    STRINGi44689.DDB0231341.

    Protein family/group databases

    CAZyiGT77. Glycosyltransferase Family 77.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0231341; DDB0231341; DDB_G0283005.
    GeneIDi8623882.
    KEGGiddi:DDB_G0283005.

    Organism-specific databases

    dictyBaseiDDB_G0283005. agtA.

    Phylogenomic databases

    eggNOGiCOG2319.
    InParanoidiQ54RP0.
    OMAiSSINCIH.
    PhylomeDBiQ54RP0.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    SABIO-RKQ54RP0.

    Miscellaneous databases

    PROiQ54RP0.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR005069. Nucl-diP-sugar_transferase.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03407. Nucleotid_trans. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular characterization of a novel UDP-galactose:fucoside alpha3-galactosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
      Ercan A., Panico M., Sutton-Smith M., Dell A., Morris H.R., Matta K.L., Gay D.F., West C.M.
      J. Biol. Chem. 281:12713-12721(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 95-100; 246-251; 336-343; 352-357; 390-397 AND 492-499, FUNCTION, DOMAIN, DISRUPTION PHENOTYPE.
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium."
      Ketcham C., Wang F., Fisher S.Z., Ercan A., van der Wel H., Locke R.D., Sirajud-Doulah K., Matta K.L., West C.M.
      J. Biol. Chem. 279:29050-29059(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: AX3.

    Entry informationi

    Entry nameiAGTA_DICDI
    AccessioniPrimary (citable) accession number: Q54RP0
    Secondary accession number(s): Q2LAG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: June 24, 2015
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Not required for growth.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.