Q54RP0 (AGTA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-galactose:fucoside alpha-3-galactosyltransferase
EC=2.4.1.37

Alternative name(s):
Alpha-GalT1
Fucosylgalactoside 3-alpha-galactosyltransferase
Skp1 alpha-3-galactosyltransferase

Gene names

Name:	agtA
Synonyms:	GT78
ORF Names:	DDB_G0283005

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically catalyzes the transfer of a galactosyl residue to the hydroxyproline-linked saccharide on Skp1 protein (fpaA/fpaB). Catalyzes the formation of a Gal-alpha-1,3-Fuc linkage, leading to Gal-Fuc-Gal-GlcNAc-HyPro143-Skp1. Ref.1 Ref.3
Catalytic activity	UDP-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R. Ref.3
Cofactor	Divalent metal cations. Mn2+ is 4-fold better than Mg2+. Ref.3
Enzyme regulation	Stimulated by dithiothreitol (DTT) in vitro. Totally inhibited by EDTA. Ref.3
Pathway	Protein modification; protein glycosylation.
Subcellular location	Cytoplasm Ref.3.
Domain	Consists of two domains: an N-terminal catalytic domain and a C-terminal domain, not required for activity, that is predicted to form a beta-propeller-like protein-protein interaction domain. Ref.1
Disruption phenotype	Cells show an accumulation of the non-galactosylated isoform of Skp1 in cells. Ref.1
Miscellaneous	Not required for growth.
Sequence similarities	Belongs to the glycosyltransferase 77 family. Contains 7 WD repeats.
Biophysicochemical properties	Kinetic parameters: The catalytic efficiency with the presumed natural substrate Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 is 21-fold and 21000-fold higher than that of the synthetic substrates Fuc-alpha-1,2-Gal-beta-1-pNP and Fuc-alpha-1-Bn, respectively. KM=3.5 µM for UDP-Gal Ref.3 KM=3.8 mM for Fuc-alpha-1-Bn KM=0.84 mM for Fuc-alpha-1,2-Gal-beta-1-Bn KM=0.82 mM for Fuc-alpha-1,2-Gal-beta-1-pNP KM=0.006 mM for Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 pH dependence: Optimum pH is 6.4-7.4. Temperature dependence: Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat WD repeat
Ligand	Magnesium Manganese
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from direct assay Ref.1. Source: dictyBase
Cellular component	cytoplasm Traceable author statement Ref.3. Source: dictyBase
Molecular function	fucosylgalactoside 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	UDP-galactose:fucoside alpha-3-galactosyltransferase		PRO_0000328554
Regions
	Repeat	320 – 358	39	WD 1
	Repeat	372 – 420	49	WD 2
	Repeat	422 – 461	40	WD 3
	Repeat	464 – 505	42	WD 4
	Repeat	507 – 546	40	WD 5
	Repeat	556 – 595	40	WD 6
	Repeat	617 – 648	32	WD 7
	Compositional bias	168 – 171	4	Poly-Asp
	Compositional bias	386 – 391	6	Poly-Asn
	Compositional bias	590 – 604	15	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q54RP0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 1A92113B7C48E9D4	FASTA	648	75,244
	10 20 30 40 50 60 MEKKVEYIKE NDKIVLMCNY GFRDMTLNLL KCFEKLSIDK SRYILYCMDD KAYQFFAEFK 70 80 90 100 110 120 GIECQRFSRD DIINSSTSST QLFHDNNTND NKGIYSENAE SYGDIGFRAI CNEKPLVVLD 130 140 150 160 170 180 VLKKGYNVLW TDTDIVWKRD PFIHFYQDIN QENQFTNDDD IDLYVQQDDD DICAGFYFIR 190 200 210 220 230 240 SNQRTIKFIQ DSINFLNPCI DDQIAMRLFL KSQGINIKSK NILLSLSEND KKDKIRYRLL 250 260 270 280 290 300 DKKLFPNGTN YFNLKITQRD NITPFIIHNN CIIGHRSKKD RFIEYGLWYI NDDEIDINSN 310 320 330 340 350 360 INNDDENNKE IKLFKNVLKN HTDIITSINS SDDGKLFTTS IDKSIKIWKF ENTSGNDTDN 370 380 390 400 410 420 GIFKVLKSKY IHKRGGIWST FIFSSNNNNN NDYGFENLLT SSHDKTIQYW DNNLQVIQTF 430 440 450 460 470 480 IGHTGIINQL IVIPNSSYFF TCSDDNTIRQ FDLNNINFKR VFIGHNGWVS SIAINKNLNT 490 500 510 520 530 540 LYSCSNDGTI RFWDINSGRC LNIIKGNQGG WIRKIIYNDN LNQLISGGND GTIKIWSCDN 550 560 570 580 590 600 LNNFNDNQYL LKINTNENSS INDLQFDSDT NLIYCAFENG SLKSFKLTSN NNNNNNNNNN 610 620 630 640 NNNNTINYSL DKVYQGHLNS SINCIHISKS LNLLFSGGFD KQIKSWDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a novel UDP-galactose:fucoside alpha3-galactosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium." Ercan A., Panico M., Sutton-Smith M., Dell A., Morris H.R., Matta K.L., Gay D.F., West C.M. J. Biol. Chem. 281:12713-12721(2006) [PubMed: 16495217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 95-100; 246-251; 336-343; 352-357; 390-397 AND 492-499, FUNCTION, DOMAIN, DISRUPTION PHENOTYPE. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium." Ketcham C., Wang F., Fisher S.Z., Ercan A., van der Wel H., Locke R.D., Sirajud-Doulah K., Matta K.L., West C.M. J. Biol. Chem. 279:29050-29059(2004) [PubMed: 15123660] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ340632 Genomic DNA. Translation: ABC67744.1. AAFI02000049 Genomic DNA. Translation: EAL65946.1.
RefSeq	XP_639311.1. XM_634219.1.
3D structure databases
ProteinModelPortal	Q54RP0.
ModBase	Search...
Protein family/group databases
CAZy	GT77. Glycosyltransferase Family 77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231341; DDB0231341; DDB_G0283005.
GeneID	8623882.
GenomeReviews	Gene locus agtA in contig CM000153_GR.
KEGG	ddi:DDB_G0283005.
Organism-specific databases
dictyBase	DDB_G0283005. agtA.
Phylogenomic databases
eggNOG	KOG0263.
GeneTree	EPrGT00050000000798.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR005069. Nucl-diP-sugar_transferase. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF03407. Nucleotid_trans. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGTA_DICDI

Accession

Primary (citable) accession number: Q54RP0
Secondary accession number(s): Q2LAG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)