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Q54RP0

- AGTA_DICDI

UniProt

Q54RP0 - AGTA_DICDI

Protein

UDP-galactose:fucoside alpha-3-galactosyltransferase

Gene

agtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Specifically catalyzes the transfer of a galactosyl residue to the hydroxyproline-linked saccharide on Skp1 protein (fpaA/fpaB). Catalyzes the formation of a Gal-alpha-1,3-Fuc linkage, leading to Gal-Fuc-Gal-GlcNAc-HyPro143-Skp1.2 Publications

    Catalytic activityi

    UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.1 Publication

    Cofactori

    Divalent metal cations. Mn2+ is 4-fold better than Mg2+.1 Publication

    Enzyme regulationi

    Stimulated by dithiothreitol (DTT) in vitro. Totally inhibited by EDTA.1 Publication

    Kineticsi

    The catalytic efficiency with the presumed natural substrate Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 is 21-fold and 21000-fold higher than that of the synthetic substrates Fuc-alpha-1,2-Gal-beta-1-pNP and Fuc-alpha-1-Bn, respectively.

    1. KM=3.5 µM for UDP-Gal1 Publication
    2. KM=3.8 mM for Fuc-alpha-1-Bn1 Publication
    3. KM=0.84 mM for Fuc-alpha-1,2-Gal-beta-1-Bn1 Publication
    4. KM=0.82 mM for Fuc-alpha-1,2-Gal-beta-1-pNP1 Publication
    5. KM=0.006 mM for Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp11 Publication

    pH dependencei

    Optimum pH is 6.4-7.4.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
    2. UDP-galactosyltransferase activity Source: dictyBase

    GO - Biological processi

    1. protein glycosylation Source: dictyBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Manganese

    Enzyme and pathway databases

    SABIO-RKQ54RP0.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT77. Glycosyltransferase Family 77.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-galactose:fucoside alpha-3-galactosyltransferase (EC:2.4.1.37)
    Alternative name(s):
    Alpha-GalT1
    Fucosylgalactoside 3-alpha-galactosyltransferase
    Skp1 alpha-3-galactosyltransferase
    Gene namesi
    Name:agtA
    Synonyms:GT78
    ORF Names:DDB_G0283005
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283005. agtA.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells show an accumulation of the non-galactosylated isoform of Skp1 in cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648UDP-galactose:fucoside alpha-3-galactosyltransferasePRO_0000328554Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi1248885. 1 interaction.
    STRINGi44689.DDB_0231341.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54RP0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati320 – 35839WD 1Add
    BLAST
    Repeati372 – 42049WD 2Add
    BLAST
    Repeati422 – 46140WD 3Add
    BLAST
    Repeati464 – 50542WD 4Add
    BLAST
    Repeati507 – 54640WD 5Add
    BLAST
    Repeati556 – 59540WD 6Add
    BLAST
    Repeati617 – 64832WD 7Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi168 – 1714Poly-Asp
    Compositional biasi386 – 3916Poly-Asn
    Compositional biasi590 – 60415Poly-AsnAdd
    BLAST

    Domaini

    Consists of two domains: an N-terminal catalytic domain and a C-terminal domain, not required for activity, that is predicted to form a beta-propeller-like protein-protein interaction domain.1 Publication

    Sequence similaritiesi

    Belongs to the glycosyltransferase 77 family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    OMAiSSINCIH.
    PhylomeDBiQ54RP0.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR005069. Nucl-diP-sugar_transferase.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF03407. Nucleotid_trans. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54RP0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKKVEYIKE NDKIVLMCNY GFRDMTLNLL KCFEKLSIDK SRYILYCMDD    50
    KAYQFFAEFK GIECQRFSRD DIINSSTSST QLFHDNNTND NKGIYSENAE 100
    SYGDIGFRAI CNEKPLVVLD VLKKGYNVLW TDTDIVWKRD PFIHFYQDIN 150
    QENQFTNDDD IDLYVQQDDD DICAGFYFIR SNQRTIKFIQ DSINFLNPCI 200
    DDQIAMRLFL KSQGINIKSK NILLSLSEND KKDKIRYRLL DKKLFPNGTN 250
    YFNLKITQRD NITPFIIHNN CIIGHRSKKD RFIEYGLWYI NDDEIDINSN 300
    INNDDENNKE IKLFKNVLKN HTDIITSINS SDDGKLFTTS IDKSIKIWKF 350
    ENTSGNDTDN GIFKVLKSKY IHKRGGIWST FIFSSNNNNN NDYGFENLLT 400
    SSHDKTIQYW DNNLQVIQTF IGHTGIINQL IVIPNSSYFF TCSDDNTIRQ 450
    FDLNNINFKR VFIGHNGWVS SIAINKNLNT LYSCSNDGTI RFWDINSGRC 500
    LNIIKGNQGG WIRKIIYNDN LNQLISGGND GTIKIWSCDN LNNFNDNQYL 550
    LKINTNENSS INDLQFDSDT NLIYCAFENG SLKSFKLTSN NNNNNNNNNN 600
    NNNNTINYSL DKVYQGHLNS SINCIHISKS LNLLFSGGFD KQIKSWDL 648
    Length:648
    Mass (Da):75,244
    Last modified:May 24, 2005 - v1
    Checksum:i1A92113B7C48E9D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ340632 Genomic DNA. Translation: ABC67744.1.
    AAFI02000049 Genomic DNA. Translation: EAL65946.1.
    RefSeqiXP_639311.1. XM_634219.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231341; DDB0231341; DDB_G0283005.
    GeneIDi8623882.
    KEGGiddi:DDB_G0283005.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ340632 Genomic DNA. Translation: ABC67744.1 .
    AAFI02000049 Genomic DNA. Translation: EAL65946.1 .
    RefSeqi XP_639311.1. XM_634219.1.

    3D structure databases

    ProteinModelPortali Q54RP0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1248885. 1 interaction.
    STRINGi 44689.DDB_0231341.

    Protein family/group databases

    CAZyi GT77. Glycosyltransferase Family 77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231341 ; DDB0231341 ; DDB_G0283005 .
    GeneIDi 8623882.
    KEGGi ddi:DDB_G0283005.

    Organism-specific databases

    dictyBasei DDB_G0283005. agtA.

    Phylogenomic databases

    eggNOGi COG2319.
    OMAi SSINCIH.
    PhylomeDBi Q54RP0.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    SABIO-RK Q54RP0.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR005069. Nucl-diP-sugar_transferase.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF03407. Nucleotid_trans. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a novel UDP-galactose:fucoside alpha3-galactosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
      Ercan A., Panico M., Sutton-Smith M., Dell A., Morris H.R., Matta K.L., Gay D.F., West C.M.
      J. Biol. Chem. 281:12713-12721(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 95-100; 246-251; 336-343; 352-357; 390-397 AND 492-499, FUNCTION, DOMAIN, DISRUPTION PHENOTYPE.
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium."
      Ketcham C., Wang F., Fisher S.Z., Ercan A., van der Wel H., Locke R.D., Sirajud-Doulah K., Matta K.L., West C.M.
      J. Biol. Chem. 279:29050-29059(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: AX3.

    Entry informationi

    Entry nameiAGTA_DICDI
    AccessioniPrimary (citable) accession number: Q54RP0
    Secondary accession number(s): Q2LAG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Not required for growth.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3