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Q54RP0 (AGTA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-galactose:fucoside alpha-3-galactosyltransferase

EC=2.4.1.37
Alternative name(s):
Alpha-GalT1
Fucosylgalactoside 3-alpha-galactosyltransferase
Skp1 alpha-3-galactosyltransferase
Gene names
Name:agtA
Synonyms:GT78
ORF Names:DDB_G0283005
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically catalyzes the transfer of a galactosyl residue to the hydroxyproline-linked saccharide on Skp1 protein (fpaA/fpaB). Catalyzes the formation of a Gal-alpha-1,3-Fuc linkage, leading to Gal-Fuc-Gal-GlcNAc-HyPro143-Skp1. Ref.1 Ref.3

Catalytic activity

UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R. Ref.3

Cofactor

Divalent metal cations. Mn2+ is 4-fold better than Mg2+. Ref.3

Enzyme regulation

Stimulated by dithiothreitol (DTT) in vitro. Totally inhibited by EDTA. Ref.3

Pathway

Protein modification; protein glycosylation.

Subcellular location

Cytoplasm Ref.3.

Domain

Consists of two domains: an N-terminal catalytic domain and a C-terminal domain, not required for activity, that is predicted to form a beta-propeller-like protein-protein interaction domain. Ref.1

Disruption phenotype

Cells show an accumulation of the non-galactosylated isoform ofSkp1 in cells. Ref.1

Miscellaneous

Not required for growth.

Sequence similarities

Belongs to the glycosyltransferase 77 family.

Contains 7 WD repeats.

Biophysicochemical properties

Kinetic parameters:

The catalytic efficiency with the presumed natural substrate Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 is 21-fold and 21000-fold higher than that of the synthetic substrates Fuc-alpha-1,2-Gal-beta-1-pNP and Fuc-alpha-1-Bn, respectively.

KM=3.5 µM for UDP-Gal Ref.3

KM=3.8 mM for Fuc-alpha-1-Bn

KM=0.84 mM for Fuc-alpha-1,2-Gal-beta-1-Bn

KM=0.82 mM for Fuc-alpha-1,2-Gal-beta-1-pNP

KM=0.006 mM for Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1

pH dependence:

Optimum pH is 6.4-7.4.

Temperature dependence:

Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRepeat
WD repeat
   LigandMagnesium
Manganese
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from direct assay Ref.1. Source: dictyBase

   Cellular_componentcytoplasm

Traceable author statement Ref.3. Source: dictyBase

   Molecular_functionUDP-galactosyltransferase activity

Inferred from direct assay Ref.1. Source: dictyBase

fucosylgalactoside 3-alpha-galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648UDP-galactose:fucoside alpha-3-galactosyltransferase
PRO_0000328554

Regions

Repeat320 – 35839WD 1
Repeat372 – 42049WD 2
Repeat422 – 46140WD 3
Repeat464 – 50542WD 4
Repeat507 – 54640WD 5
Repeat556 – 59540WD 6
Repeat617 – 64832WD 7
Compositional bias168 – 1714Poly-Asp
Compositional bias386 – 3916Poly-Asn
Compositional bias590 – 60415Poly-Asn

Sequences

Sequence LengthMass (Da)Tools
Q54RP0 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 1A92113B7C48E9D4

FASTA64875,244
        10         20         30         40         50         60 
MEKKVEYIKE NDKIVLMCNY GFRDMTLNLL KCFEKLSIDK SRYILYCMDD KAYQFFAEFK 

        70         80         90        100        110        120 
GIECQRFSRD DIINSSTSST QLFHDNNTND NKGIYSENAE SYGDIGFRAI CNEKPLVVLD 

       130        140        150        160        170        180 
VLKKGYNVLW TDTDIVWKRD PFIHFYQDIN QENQFTNDDD IDLYVQQDDD DICAGFYFIR 

       190        200        210        220        230        240 
SNQRTIKFIQ DSINFLNPCI DDQIAMRLFL KSQGINIKSK NILLSLSEND KKDKIRYRLL 

       250        260        270        280        290        300 
DKKLFPNGTN YFNLKITQRD NITPFIIHNN CIIGHRSKKD RFIEYGLWYI NDDEIDINSN 

       310        320        330        340        350        360 
INNDDENNKE IKLFKNVLKN HTDIITSINS SDDGKLFTTS IDKSIKIWKF ENTSGNDTDN 

       370        380        390        400        410        420 
GIFKVLKSKY IHKRGGIWST FIFSSNNNNN NDYGFENLLT SSHDKTIQYW DNNLQVIQTF 

       430        440        450        460        470        480 
IGHTGIINQL IVIPNSSYFF TCSDDNTIRQ FDLNNINFKR VFIGHNGWVS SIAINKNLNT 

       490        500        510        520        530        540 
LYSCSNDGTI RFWDINSGRC LNIIKGNQGG WIRKIIYNDN LNQLISGGND GTIKIWSCDN 

       550        560        570        580        590        600 
LNNFNDNQYL LKINTNENSS INDLQFDSDT NLIYCAFENG SLKSFKLTSN NNNNNNNNNN 

       610        620        630        640 
NNNNTINYSL DKVYQGHLNS SINCIHISKS LNLLFSGGFD KQIKSWDL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of a novel UDP-galactose:fucoside alpha3-galactosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
Ercan A., Panico M., Sutton-Smith M., Dell A., Morris H.R., Matta K.L., Gay D.F., West C.M.
J. Biol. Chem. 281:12713-12721(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 95-100; 246-251; 336-343; 352-357; 390-397 AND 492-499, FUNCTION, DOMAIN, DISRUPTION PHENOTYPE.
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium."
Ketcham C., Wang F., Fisher S.Z., Ercan A., van der Wel H., Locke R.D., Sirajud-Doulah K., Matta K.L., West C.M.
J. Biol. Chem. 279:29050-29059(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
Strain: AX3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ340632 Genomic DNA. Translation: ABC67744.1.
AAFI02000049 Genomic DNA. Translation: EAL65946.1.
RefSeqXP_639311.1. XM_634219.1.

3D structure databases

ProteinModelPortalQ54RP0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid1248885. 1 interaction.
STRING44689.DDB_0231341.

Protein family/group databases

CAZyGT77. Glycosyltransferase Family 77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231341; DDB0231341; DDB_G0283005.
GeneID8623882.
KEGGddi:DDB_G0283005.

Organism-specific databases

dictyBaseDDB_G0283005. agtA.

Phylogenomic databases

eggNOGCOG2319.
OMASSINCIH.
PhylomeDBQ54RP0.

Enzyme and pathway databases

SABIO-RKQ54RP0.
UniPathwayUPA00378.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR005069. Nucl-diP-sugar_transferase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF03407. Nucleotid_trans. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGTA_DICDI
AccessionPrimary (citable) accession number: Q54RP0
Secondary accession number(s): Q2LAG6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase