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Q54RP0

- AGTA_DICDI

UniProt

Q54RP0 - AGTA_DICDI

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Protein

UDP-galactose:fucoside alpha-3-galactosyltransferase

Gene
agtA, GT78, DDB_G0283005
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically catalyzes the transfer of a galactosyl residue to the hydroxyproline-linked saccharide on Skp1 protein (fpaA/fpaB). Catalyzes the formation of a Gal-alpha-1,3-Fuc linkage, leading to Gal-Fuc-Gal-GlcNAc-HyPro143-Skp1.2 Publications

Catalytic activityi

UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.1 Publication

Cofactori

Divalent metal cations. Mn2+ is 4-fold better than Mg2+.1 Publication

Enzyme regulationi

Stimulated by dithiothreitol (DTT) in vitro. Totally inhibited by EDTA.1 Publication

Kineticsi

The catalytic efficiency with the presumed natural substrate Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1 is 21-fold and 21000-fold higher than that of the synthetic substrates Fuc-alpha-1,2-Gal-beta-1-pNP and Fuc-alpha-1-Bn, respectively.

  1. KM=3.5 µM for UDP-Gal1 Publication
  2. KM=3.8 mM for Fuc-alpha-1-Bn
  3. KM=0.84 mM for Fuc-alpha-1,2-Gal-beta-1-Bn
  4. KM=0.82 mM for Fuc-alpha-1,2-Gal-beta-1-pNP
  5. KM=0.006 mM for Fuc-alpha-1,2-Gal-beta-1,3-GlcNAc-alpha-1-Skp1

pH dependencei

Optimum pH is 6.4-7.4.

Temperature dependencei

Optimum temperature is 37 degrees Celsius.

Pathwayi

GO - Molecular functioni

  1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
  2. UDP-galactosyltransferase activity Source: dictyBase

GO - Biological processi

  1. protein glycosylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Manganese

Enzyme and pathway databases

SABIO-RKQ54RP0.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT77. Glycosyltransferase Family 77.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-galactose:fucoside alpha-3-galactosyltransferase (EC:2.4.1.37)
Alternative name(s):
Alpha-GalT1
Fucosylgalactoside 3-alpha-galactosyltransferase
Skp1 alpha-3-galactosyltransferase
Gene namesi
Name:agtA
Synonyms:GT78
ORF Names:DDB_G0283005
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283005. agtA.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells show an accumulation of the non-galactosylated isoform of Skp1 in cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648UDP-galactose:fucoside alpha-3-galactosyltransferasePRO_0000328554Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi1248885. 1 interaction.
STRINGi44689.DDB_0231341.

Structurei

3D structure databases

ProteinModelPortaliQ54RP0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati320 – 35839WD 1Add
BLAST
Repeati372 – 42049WD 2Add
BLAST
Repeati422 – 46140WD 3Add
BLAST
Repeati464 – 50542WD 4Add
BLAST
Repeati507 – 54640WD 5Add
BLAST
Repeati556 – 59540WD 6Add
BLAST
Repeati617 – 64832WD 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi168 – 1714Poly-Asp
Compositional biasi386 – 3916Poly-Asn
Compositional biasi590 – 60415Poly-AsnAdd
BLAST

Domaini

Consists of two domains: an N-terminal catalytic domain and a C-terminal domain, not required for activity, that is predicted to form a beta-propeller-like protein-protein interaction domain.1 Publication

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
OMAiSSINCIH.
PhylomeDBiQ54RP0.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR005069. Nucl-diP-sugar_transferase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03407. Nucleotid_trans. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54RP0-1 [UniParc]FASTAAdd to Basket

« Hide

MEKKVEYIKE NDKIVLMCNY GFRDMTLNLL KCFEKLSIDK SRYILYCMDD    50
KAYQFFAEFK GIECQRFSRD DIINSSTSST QLFHDNNTND NKGIYSENAE 100
SYGDIGFRAI CNEKPLVVLD VLKKGYNVLW TDTDIVWKRD PFIHFYQDIN 150
QENQFTNDDD IDLYVQQDDD DICAGFYFIR SNQRTIKFIQ DSINFLNPCI 200
DDQIAMRLFL KSQGINIKSK NILLSLSEND KKDKIRYRLL DKKLFPNGTN 250
YFNLKITQRD NITPFIIHNN CIIGHRSKKD RFIEYGLWYI NDDEIDINSN 300
INNDDENNKE IKLFKNVLKN HTDIITSINS SDDGKLFTTS IDKSIKIWKF 350
ENTSGNDTDN GIFKVLKSKY IHKRGGIWST FIFSSNNNNN NDYGFENLLT 400
SSHDKTIQYW DNNLQVIQTF IGHTGIINQL IVIPNSSYFF TCSDDNTIRQ 450
FDLNNINFKR VFIGHNGWVS SIAINKNLNT LYSCSNDGTI RFWDINSGRC 500
LNIIKGNQGG WIRKIIYNDN LNQLISGGND GTIKIWSCDN LNNFNDNQYL 550
LKINTNENSS INDLQFDSDT NLIYCAFENG SLKSFKLTSN NNNNNNNNNN 600
NNNNTINYSL DKVYQGHLNS SINCIHISKS LNLLFSGGFD KQIKSWDL 648
Length:648
Mass (Da):75,244
Last modified:May 24, 2005 - v1
Checksum:i1A92113B7C48E9D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ340632 Genomic DNA. Translation: ABC67744.1.
AAFI02000049 Genomic DNA. Translation: EAL65946.1.
RefSeqiXP_639311.1. XM_634219.1.

Genome annotation databases

EnsemblProtistsiDDB0231341; DDB0231341; DDB_G0283005.
GeneIDi8623882.
KEGGiddi:DDB_G0283005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ340632 Genomic DNA. Translation: ABC67744.1 .
AAFI02000049 Genomic DNA. Translation: EAL65946.1 .
RefSeqi XP_639311.1. XM_634219.1.

3D structure databases

ProteinModelPortali Q54RP0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 1248885. 1 interaction.
STRINGi 44689.DDB_0231341.

Protein family/group databases

CAZyi GT77. Glycosyltransferase Family 77.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231341 ; DDB0231341 ; DDB_G0283005 .
GeneIDi 8623882.
KEGGi ddi:DDB_G0283005.

Organism-specific databases

dictyBasei DDB_G0283005. agtA.

Phylogenomic databases

eggNOGi COG2319.
OMAi SSINCIH.
PhylomeDBi Q54RP0.

Enzyme and pathway databases

UniPathwayi UPA00378 .
SABIO-RK Q54RP0.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR005069. Nucl-diP-sugar_transferase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF03407. Nucleotid_trans. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a novel UDP-galactose:fucoside alpha3-galactosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
    Ercan A., Panico M., Sutton-Smith M., Dell A., Morris H.R., Matta K.L., Gay D.F., West C.M.
    J. Biol. Chem. 281:12713-12721(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 95-100; 246-251; 336-343; 352-357; 390-397 AND 492-499, FUNCTION, DOMAIN, DISRUPTION PHENOTYPE.
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium."
    Ketcham C., Wang F., Fisher S.Z., Ercan A., van der Wel H., Locke R.D., Sirajud-Doulah K., Matta K.L., West C.M.
    J. Biol. Chem. 279:29050-29059(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: AX3.

Entry informationi

Entry nameiAGTA_DICDI
AccessioniPrimary (citable) accession number: Q54RP0
Secondary accession number(s): Q2LAG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Not required for growth.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3