Reviewed, UniProtKB/Swiss-Prot Q54RK5 (ENOA_DICDI)

Names and origin

Protein names

Recommended name:
    Enolase A
    EC=4.2.1.11
Alternative name(s):
    2-phosphoglycerate dehydratase A
    2-phospho-D-glycerate hydro-lyase A

Gene names

Name:	enoA
ORF Names:	DDB_G0283137

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro sexual reproduction Inferred from expression pattern. Source: dictyBase
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Enolase A		PRO_0000330350
Regions
	Region	373 – 376	4	Substrate binding By similarity
Sites
	Active site	212	1	Proton donor By similarity
	Active site	346	1	Proton acceptor By similarity
	Metal binding	247	1	Magnesium By similarity
	Metal binding	296	1	Magnesium By similarity
	Metal binding	321	1	Magnesium By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	296	1	Substrate By similarity
	Binding site	321	1	Substrate By similarity
	Binding site	397	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54RK5-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 9AA4156D859BC896	FASTA	434	46,991
	10 20 30 40 50 60 MSVIKSIKAR EILNSRGNPT VEVDLYTEKD GVVSSFRAAV PSGASTGIYE AVELRDGDKS 70 80 90 100 110 120 RYLGKGVLKA IKNILEVIQP AVIGKSVSDQ AAIDKLMIDL DGTPNKGKLG ANAILAVSLA 130 140 150 160 170 180 VCRAGAADRN LPLYKYISEI AGTKMRLPVP AFNVINGGSH AGNKLAMQEF MILPVGAKDF 190 200 210 220 230 240 NEAYRMGSEV YHNLKNVISG RYGQDAINVG DEGGFAPPIQ SNKEGLELLK LAIEKAGYTG 250 260 270 280 290 300 LVKIGMDCAA SEFKVENGYD LDFKTKNNDG SAVISGEKLG DLYREFIKEY PIISIEDPFD 310 320 330 340 350 360 QDDWESYTKL TASVDIQIVG DDLLVTNPER IKTGIEKKAC NALLLKVNQI GSVTESIRAA 370 380 390 400 410 420 LDSKNASWGV MVSHRSGETE DTFIADLVVG LGTGQIKTGA PCRSERLAKY NQLVRINEEL 430 GENHNYAGLT FRKF

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000050 Genomic DNA. Translation: EAL65898.1.
RefSeq	XP_639231.1.
3D structure databases
SMR	Q54RK5. Positions 4-432.
ModBase	Search...
Genome annotation databases
GeneID	3390815.
KEGG	ddi:DDB_0231355.
Organism-specific databases
dictyBase	DDB_G0283137. enoA.
Family and domain databases
InterPro	IPR000941. Enolase. [Graphical view]
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
ProDom	PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENOA_DICDI

Accession

Primary (citable) accession number: Q54RK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)