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Protein

Enolase A

Gene

enoA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA)
  2. Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. Enolase A (enoA), Enolase B (enoB)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi321MagnesiumBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei346Proton acceptorBy similarity1
Binding sitei397SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • sexual reproduction Source: dictyBase

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-70171. Glycolysis.
R-DDI-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase A (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase A
2-phosphoglycerate dehydratase A
Gene namesi
Name:enoA
ORF Names:DDB_G0283137
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283137. enoA.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003303501 – 434Enolase AAdd BLAST434

Proteomic databases

PaxDbiQ54RK5.
PRIDEiQ54RK5.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231355.

Structurei

3D structure databases

ProteinModelPortaliQ54RK5.
SMRiQ54RK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni373 – 376Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
InParanoidiQ54RK5.
KOiK01689.
OMAiEFMIIPV.
PhylomeDBiQ54RK5.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54RK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIKSIKAR EILNSRGNPT VEVDLYTEKD GVVSSFRAAV PSGASTGIYE
60 70 80 90 100
AVELRDGDKS RYLGKGVLKA IKNILEVIQP AVIGKSVSDQ AAIDKLMIDL
110 120 130 140 150
DGTPNKGKLG ANAILAVSLA VCRAGAADRN LPLYKYISEI AGTKMRLPVP
160 170 180 190 200
AFNVINGGSH AGNKLAMQEF MILPVGAKDF NEAYRMGSEV YHNLKNVISG
210 220 230 240 250
RYGQDAINVG DEGGFAPPIQ SNKEGLELLK LAIEKAGYTG LVKIGMDCAA
260 270 280 290 300
SEFKVENGYD LDFKTKNNDG SAVISGEKLG DLYREFIKEY PIISIEDPFD
310 320 330 340 350
QDDWESYTKL TASVDIQIVG DDLLVTNPER IKTGIEKKAC NALLLKVNQI
360 370 380 390 400
GSVTESIRAA LDSKNASWGV MVSHRSGETE DTFIADLVVG LGTGQIKTGA
410 420 430
PCRSERLAKY NQLVRINEEL GENHNYAGLT FRKF
Length:434
Mass (Da):46,991
Last modified:May 24, 2005 - v1
Checksum:i9AA4156D859BC896
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000050 Genomic DNA. Translation: EAL65898.1.
RefSeqiXP_639231.1. XM_634139.1.

Genome annotation databases

EnsemblProtistsiEAL65898; EAL65898; DDB_G0283137.
GeneIDi8623917.
KEGGiddi:DDB_G0283137.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENOA_DICDI
AccessioniPrimary (citable) accession number: Q54RK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: June 7, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families