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Q54RF5

- KPYK_DICDI

UniProt

Q54RF5 - KPYK_DICDI

Protein

Pyruvate kinase

Gene

pyk

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501SubstrateBy similarity
    Metal bindingi52 – 521PotassiumBy similarity
    Metal bindingi54 – 541PotassiumBy similarity
    Metal bindingi84 – 841PotassiumBy similarity
    Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
    Sitei240 – 2401Transition state stabilizerBy similarity
    Metal bindingi242 – 2421MagnesiumBy similarity
    Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
    Metal bindingi266 – 2661MagnesiumBy similarity
    Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
    Binding sitei298 – 2981SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: dictyBase

    GO - Biological processi

    1. glycolytic process Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    ReactomeiREACT_212128. Regulation of gene expression in beta cells.
    REACT_219906. ChREBP activates metabolic gene expression.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    ORF Names:DDB_G0283247
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283247. pyk.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Pyruvate kinasePRO_0000327689Add
    BLAST

    Proteomic databases

    PRIDEiQ54RF5.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231421.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54RF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    KOiK00873.
    OMAiTHEDHKE.
    PhylomeDBiQ54RF5.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54RF5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLSRNLRL SLDTPTSTFV RTKIVCTIGP KTMSEEALIK LIETGMNVCR    50
    LNFSHGTHDY HGQVIKNVRS AMEKTGKIIA IMLDTKGPEI RTGKIEDRCG 100
    YVDLFVGQEI LVDTNMNQPG TSFRISIDYK GLLDSVKVGG YILIADGVIS 150
    LSITAVEKEK GHVVCRVNNN SRLGENKNVH LPGAIVNLPA VSEKDILDIK 200
    FGVEQNVDFI AASFIRKADD VNEIREILGE KGKDIQIISK IENVEGVDNF 250
    NEILEVSDGI MVARGDLGVE VQMEKIFVAQ KMIVSKCNAA GKPVITATQM 300
    LESMIKNPRP TRAEATDVAN AVLDGSDCVM LSGETASGDY PYEAVDIMAK 350
    ICREAELVES STDYQTLFAA LKLSSAKPVS IAETVASYAV ATAIDLKADL 400
    IITLTETGLT ARLVSKYRPS IPIIAVTSWS YTVKHLLATR GAIPFLVESL 450
    VGTDKLVESC LEYAMKHNLC KKGSRVVIVS GVMEGVPGKT NSLRVLTVGE 500
    SIKDLKV 507
    Length:507
    Mass (Da):54,999
    Last modified:May 24, 2005 - v1
    Checksum:iAC93DBF9A53B705D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000051 Genomic DNA. Translation: EAL65862.1.
    RefSeqiXP_639190.1. XM_634098.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231421; DDB0231421; DDB_G0283247.
    GeneIDi8623966.
    KEGGiddi:DDB_G0283247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000051 Genomic DNA. Translation: EAL65862.1 .
    RefSeqi XP_639190.1. XM_634098.1.

    3D structure databases

    ProteinModelPortali Q54RF5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231421.

    Proteomic databases

    PRIDEi Q54RF5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231421 ; DDB0231421 ; DDB_G0283247 .
    GeneIDi 8623966.
    KEGGi ddi:DDB_G0283247.

    Organism-specific databases

    dictyBasei DDB_G0283247. pyk.

    Phylogenomic databases

    eggNOGi COG0469.
    KOi K00873.
    OMAi THEDHKE.
    PhylomeDBi Q54RF5.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    Reactomei REACT_212128. Regulation of gene expression in beta cells.
    REACT_219906. ChREBP activates metabolic gene expression.

    Miscellaneous databases

    PROi Q54RF5.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Veltman D.M., Insall R.H.
      Submitted (JAN-2010) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 10-21; 78-91; 265-275 AND 476-489, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiKPYK_DICDI
    AccessioniPrimary (citable) accession number: Q54RF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3