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Q54RF5

- KPYK_DICDI

UniProt

Q54RF5 - KPYK_DICDI

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Protein
Pyruvate kinase
Gene
pyk, DDB_G0283247
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium By similarity.
Potassium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate By similarity
Metal bindingi52 – 521Potassium By similarity
Metal bindingi54 – 541Potassium By similarity
Metal bindingi84 – 841Potassium By similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygen By similarity
Sitei240 – 2401Transition state stabilizer By similarity
Metal bindingi242 – 2421Magnesium By similarity
Binding sitei265 – 2651Substrate; via amide nitrogen By similarity
Metal bindingi266 – 2661Magnesium By similarity
Binding sitei266 – 2661Substrate; via amide nitrogen By similarity
Binding sitei298 – 2981Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: dictyBase

GO - Biological processi

  1. glycolytic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_212128. Regulation of gene expression in beta cells.
REACT_219906. ChREBP activates metabolic gene expression.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
ORF Names:DDB_G0283247
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283247. pyk.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Pyruvate kinase
PRO_0000327689Add
BLAST

Proteomic databases

PRIDEiQ54RF5.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0231421.

Structurei

3D structure databases

ProteinModelPortaliQ54RF5.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
KOiK00873.
OMAiTHEDHKE.
PhylomeDBiQ54RF5.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54RF5-1 [UniParc]FASTAAdd to Basket

« Hide

MATLSRNLRL SLDTPTSTFV RTKIVCTIGP KTMSEEALIK LIETGMNVCR    50
LNFSHGTHDY HGQVIKNVRS AMEKTGKIIA IMLDTKGPEI RTGKIEDRCG 100
YVDLFVGQEI LVDTNMNQPG TSFRISIDYK GLLDSVKVGG YILIADGVIS 150
LSITAVEKEK GHVVCRVNNN SRLGENKNVH LPGAIVNLPA VSEKDILDIK 200
FGVEQNVDFI AASFIRKADD VNEIREILGE KGKDIQIISK IENVEGVDNF 250
NEILEVSDGI MVARGDLGVE VQMEKIFVAQ KMIVSKCNAA GKPVITATQM 300
LESMIKNPRP TRAEATDVAN AVLDGSDCVM LSGETASGDY PYEAVDIMAK 350
ICREAELVES STDYQTLFAA LKLSSAKPVS IAETVASYAV ATAIDLKADL 400
IITLTETGLT ARLVSKYRPS IPIIAVTSWS YTVKHLLATR GAIPFLVESL 450
VGTDKLVESC LEYAMKHNLC KKGSRVVIVS GVMEGVPGKT NSLRVLTVGE 500
SIKDLKV 507
Length:507
Mass (Da):54,999
Last modified:May 24, 2005 - v1
Checksum:iAC93DBF9A53B705D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65862.1.
RefSeqiXP_639190.1. XM_634098.1.

Genome annotation databases

EnsemblProtistsiDDB0231421; DDB0231421; DDB_G0283247.
GeneIDi8623966.
KEGGiddi:DDB_G0283247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65862.1 .
RefSeqi XP_639190.1. XM_634098.1.

3D structure databases

ProteinModelPortali Q54RF5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231421.

Proteomic databases

PRIDEi Q54RF5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231421 ; DDB0231421 ; DDB_G0283247 .
GeneIDi 8623966.
KEGGi ddi:DDB_G0283247.

Organism-specific databases

dictyBasei DDB_G0283247. pyk.

Phylogenomic databases

eggNOGi COG0469.
KOi K00873.
OMAi THEDHKE.
PhylomeDBi Q54RF5.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_212128. Regulation of gene expression in beta cells.
REACT_219906. ChREBP activates metabolic gene expression.

Miscellaneous databases

PROi Q54RF5.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Veltman D.M., Insall R.H.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 10-21; 78-91; 265-275 AND 476-489, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiKPYK_DICDI
AccessioniPrimary (citable) accession number: Q54RF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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