Q54RF5 (KPYK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	pyk
ORF Names:	DDB_G0283247

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Pyruvate kinase		PRO_0000327689
Sites
	Metal binding	52	1	Potassium By similarity
	Metal binding	54	1	Potassium By similarity
	Metal binding	84	1	Potassium By similarity
	Metal binding	85	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	242	1	Magnesium By similarity
	Metal binding	266	1	Magnesium By similarity
	Binding site	50	1	Substrate By similarity
	Binding site	265	1	Substrate; via amide nitrogen By similarity
	Binding site	266	1	Substrate; via amide nitrogen By similarity
	Binding site	298	1	Substrate By similarity
	Site	240	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54RF5 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: AC93DBF9A53B705D	FASTA	507	54,999
	10 20 30 40 50 60 MATLSRNLRL SLDTPTSTFV RTKIVCTIGP KTMSEEALIK LIETGMNVCR LNFSHGTHDY 70 80 90 100 110 120 HGQVIKNVRS AMEKTGKIIA IMLDTKGPEI RTGKIEDRCG YVDLFVGQEI LVDTNMNQPG 130 140 150 160 170 180 TSFRISIDYK GLLDSVKVGG YILIADGVIS LSITAVEKEK GHVVCRVNNN SRLGENKNVH 190 200 210 220 230 240 LPGAIVNLPA VSEKDILDIK FGVEQNVDFI AASFIRKADD VNEIREILGE KGKDIQIISK 250 260 270 280 290 300 IENVEGVDNF NEILEVSDGI MVARGDLGVE VQMEKIFVAQ KMIVSKCNAA GKPVITATQM 310 320 330 340 350 360 LESMIKNPRP TRAEATDVAN AVLDGSDCVM LSGETASGDY PYEAVDIMAK ICREAELVES 370 380 390 400 410 420 STDYQTLFAA LKLSSAKPVS IAETVASYAV ATAIDLKADL IITLTETGLT ARLVSKYRPS 430 440 450 460 470 480 IPIIAVTSWS YTVKHLLATR GAIPFLVESL VGTDKLVESC LEYAMKHNLC KKGSRVVIVS 490 500 GVMEGVPGKT NSLRVLTVGE SIKDLKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Veltman D.M., Insall R.H. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 10-21; 78-91; 265-275 AND 476-489, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000051 Genomic DNA. Translation: EAL65862.1.
RefSeq	XP_639190.1. XM_634098.1.
3D structure databases
HSSP	HSSP built from PDB template 1AQF based on UniProtKB P11974.
ProteinModelPortal	Q54RF5.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231421.
Proteomic databases
PRIDE	Q54RF5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231421; DDB0231421; DDB_G0283247.
GeneID	8623966.
KEGG	ddi:DDB_G0283247.
Organism-specific databases
dictyBase	DDB_G0283247. pyk.
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
OMA	SHVPRTK.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_DICDI

Accession

Primary (citable) accession number: Q54RF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)