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Q54RF5

- KPYK_DICDI

UniProt

Q54RF5 - KPYK_DICDI

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Protein

Pyruvate kinase

Gene

pyk

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Metal bindingi52 – 521PotassiumBy similarity
Metal bindingi54 – 541PotassiumBy similarity
Metal bindingi84 – 841PotassiumBy similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
Sitei240 – 2401Transition state stabilizerBy similarity
Metal bindingi242 – 2421MagnesiumBy similarity
Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
Metal bindingi266 – 2661MagnesiumBy similarity
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: dictyBase

GO - Biological processi

  1. glycolytic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_212128. Regulation of gene expression in beta cells.
REACT_219906. ChREBP activates metabolic gene expression.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
ORF Names:DDB_G0283247
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283247. pyk.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Pyruvate kinasePRO_0000327689Add
BLAST

Proteomic databases

PRIDEiQ54RF5.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0231421.

Structurei

3D structure databases

ProteinModelPortaliQ54RF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
InParanoidiQ54RF5.
KOiK00873.
OMAiTHEDHKE.
PhylomeDBiQ54RF5.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54RF5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATLSRNLRL SLDTPTSTFV RTKIVCTIGP KTMSEEALIK LIETGMNVCR
60 70 80 90 100
LNFSHGTHDY HGQVIKNVRS AMEKTGKIIA IMLDTKGPEI RTGKIEDRCG
110 120 130 140 150
YVDLFVGQEI LVDTNMNQPG TSFRISIDYK GLLDSVKVGG YILIADGVIS
160 170 180 190 200
LSITAVEKEK GHVVCRVNNN SRLGENKNVH LPGAIVNLPA VSEKDILDIK
210 220 230 240 250
FGVEQNVDFI AASFIRKADD VNEIREILGE KGKDIQIISK IENVEGVDNF
260 270 280 290 300
NEILEVSDGI MVARGDLGVE VQMEKIFVAQ KMIVSKCNAA GKPVITATQM
310 320 330 340 350
LESMIKNPRP TRAEATDVAN AVLDGSDCVM LSGETASGDY PYEAVDIMAK
360 370 380 390 400
ICREAELVES STDYQTLFAA LKLSSAKPVS IAETVASYAV ATAIDLKADL
410 420 430 440 450
IITLTETGLT ARLVSKYRPS IPIIAVTSWS YTVKHLLATR GAIPFLVESL
460 470 480 490 500
VGTDKLVESC LEYAMKHNLC KKGSRVVIVS GVMEGVPGKT NSLRVLTVGE

SIKDLKV
Length:507
Mass (Da):54,999
Last modified:May 24, 2005 - v1
Checksum:iAC93DBF9A53B705D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65862.1.
RefSeqiXP_639190.1. XM_634098.1.

Genome annotation databases

EnsemblProtistsiDDB0231421; DDB0231421; DDB_G0283247.
GeneIDi8623966.
KEGGiddi:DDB_G0283247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65862.1 .
RefSeqi XP_639190.1. XM_634098.1.

3D structure databases

ProteinModelPortali Q54RF5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231421.

Proteomic databases

PRIDEi Q54RF5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231421 ; DDB0231421 ; DDB_G0283247 .
GeneIDi 8623966.
KEGGi ddi:DDB_G0283247.

Organism-specific databases

dictyBasei DDB_G0283247. pyk.

Phylogenomic databases

eggNOGi COG0469.
InParanoidi Q54RF5.
KOi K00873.
OMAi THEDHKE.
PhylomeDBi Q54RF5.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_212128. Regulation of gene expression in beta cells.
REACT_219906. ChREBP activates metabolic gene expression.

Miscellaneous databases

PROi Q54RF5.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Veltman D.M., Insall R.H.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 10-21; 78-91; 265-275 AND 476-489, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiKPYK_DICDI
AccessioniPrimary (citable) accession number: Q54RF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3