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Q54RF5 (KPYK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
ORF Names:DDB_G0283247
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Pyruvate kinase
PRO_0000327689

Sites

Metal binding521Potassium By similarity
Metal binding541Potassium By similarity
Metal binding841Potassium By similarity
Metal binding851Potassium; via carbonyl oxygen By similarity
Metal binding2421Magnesium By similarity
Metal binding2661Magnesium By similarity
Binding site501Substrate By similarity
Binding site2651Substrate; via amide nitrogen By similarity
Binding site2661Substrate; via amide nitrogen By similarity
Binding site2981Substrate By similarity
Site2401Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54RF5 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: AC93DBF9A53B705D

FASTA50754,999
        10         20         30         40         50         60 
MATLSRNLRL SLDTPTSTFV RTKIVCTIGP KTMSEEALIK LIETGMNVCR LNFSHGTHDY 

        70         80         90        100        110        120 
HGQVIKNVRS AMEKTGKIIA IMLDTKGPEI RTGKIEDRCG YVDLFVGQEI LVDTNMNQPG 

       130        140        150        160        170        180 
TSFRISIDYK GLLDSVKVGG YILIADGVIS LSITAVEKEK GHVVCRVNNN SRLGENKNVH 

       190        200        210        220        230        240 
LPGAIVNLPA VSEKDILDIK FGVEQNVDFI AASFIRKADD VNEIREILGE KGKDIQIISK 

       250        260        270        280        290        300 
IENVEGVDNF NEILEVSDGI MVARGDLGVE VQMEKIFVAQ KMIVSKCNAA GKPVITATQM 

       310        320        330        340        350        360 
LESMIKNPRP TRAEATDVAN AVLDGSDCVM LSGETASGDY PYEAVDIMAK ICREAELVES 

       370        380        390        400        410        420 
STDYQTLFAA LKLSSAKPVS IAETVASYAV ATAIDLKADL IITLTETGLT ARLVSKYRPS 

       430        440        450        460        470        480 
IPIIAVTSWS YTVKHLLATR GAIPFLVESL VGTDKLVESC LEYAMKHNLC KKGSRVVIVS 

       490        500 
GVMEGVPGKT NSLRVLTVGE SIKDLKV 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Veltman D.M., Insall R.H.
Submitted (JAN-2010) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 10-21; 78-91; 265-275 AND 476-489, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000051 Genomic DNA. Translation: EAL65862.1.

3D structure databases

ProteinModelPortalQ54RF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231421.

Proteomic databases

PRIDEQ54RF5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231421; DDB0231421; DDB_G0283247.
KEGGddi:DDB_G0283247.

Organism-specific databases

dictyBaseDDB_G0283247. pyk.

Phylogenomic databases

eggNOGCOG0469.
KOK00873.
OMALEVPYYD.
PhylomeDBQ54RF5.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54RF5.

Entry information

Entry nameKPYK_DICDI
AccessionPrimary (citable) accession number: Q54RF5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase