Reviewed, UniProtKB/Swiss-Prot Q54RE8 (KMO_DICDI)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase

Gene names

Name:	kmo
ORF Names:	DDB_G0283173

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; pyridine-2,3-dicarboxylate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	kynurenine 3-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Kynurenine 3-monooxygenase		PRO_0000331234

Sequences

	Sequence		Length	Mass (Da)
	Q54RE8-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: AA02D3F26286A2A3	FASTA	460	52,261
	10 20 30 40 50 60 MDILLNKPIT IVGGGLAGSA LALLLGQKGF PIQVIEKRPK QSENIRARSI NLALSDRGVK 70 80 90 100 110 120 TLTKTGYVDD ILKIAIPMKG RMIHSLDSVQ TFQAYSSDSN KHLYSVSRQL LNDRLREHTE 130 140 150 160 170 180 KLENVKFIFS DACKSIDLKQ CTIQTQDSNQ TIEASTIIGC DGAFSAVRGS MVKLDRQDYS 190 200 210 220 230 240 QSYLKHGYKE LCIPSGPNQT FQIDKNSLHI WPRGSFMMIA LPNIDGSFTC TLFFPFDGPL 250 260 270 280 290 300 SFSSLDTREK VDQFFKDYFP DAYKLMPDLL DDYFENPTSS LVTVKTEPYH YQGKVVLVGD 310 320 330 340 350 360 AAHAIVPFYG QGMNAAFEDV LELFNCFEDK SLYPSSTDKP FDNDHFNNIY KKYQENRKAN 370 380 390 400 410 420 SDAIAEMAVE NFFEMRDHVG DALFLFKKKV EHLLEVKFPS RYISRYELIS FSTQPYAYAQ 430 440 450 460 KIGLANQQIL NELVKGNDDY NIEKIDLVLA DQLIKKYLNK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000051 Genomic DNA. Translation: EAL65825.1.
RefSeq	XP_639197.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3390740.
KEGG	ddi:DDB_0231360.
Organism-specific databases
dictyBase	DDB_G0283173. kmo.
Phylogenomic databases
OMA	Q54RE8. LHAIMPS.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_DICDI

Accession

Primary (citable) accession number: Q54RE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)