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Protein

Protein phosphatase 2A catalytic subunit B

Gene

pho2B

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Sca1 complex, a regulator of cell motility, chemotaxis and signal relay (PubMed:20493808). The Sca1 complex is recruited to the plasma membrane in a chemoattractant- and F-actin-dependent manner and is enriched at the leading edge of chemotaxing cells where it regulates F-actin dynamics and signal relay by controlling the activation of rasC and the downstream target of rapamycin complex 2 (TORC2)-Akt/protein kinase B (PKB) pathway (PubMed:20493808).1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Manganese 1By similarity1
Metal bindingi57Manganese 1By similarity1
Metal bindingi83Manganese 1By similarity1
Metal bindingi83Manganese 2By similarity1
Metal bindingi115Manganese 2By similarity1
Active sitei116Proton donorBy similarity1
Metal bindingi165Manganese 2By similarity1
Metal bindingi240Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2A catalytic subunit B1 Publication (EC:3.1.3.161 Publication)
Alternative name(s):
PP2A-C21 Publication
Gene namesi
Name:pho2B1 Publication
ORF Names:DDB_G0283187
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283187. pho2b.

Subcellular locationi

  • Cell membrane 1 Publication
  • Note: The Sca1 complex is recruited to the plasma membrane in a chemoattractant- and F-actin-dependent manner and is enriched at the leading edge of chemotaxing cells (PubMed:20493808). Membrane localization of the Sca1 complex is regulated by scaA phosphorylation by PKB and PKB-related PKBR1 (PubMed:20493808).1 Publication

GO - Cellular componenti

  • plasma membrane Source: UniProtKB-SubCell
  • Ras guanyl-nucleotide exchange factor complex Source: dictyBase

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003682071 – 312Protein phosphatase 2A catalytic subunit BAdd BLAST312

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ54RD6.

Interactioni

Subunit structurei

Component of the Sca1 complex composed of at least gefA, gefH, scaA, phr, and the protein phosphatase 2A subunits pppA and pho2B (PubMed:20493808).1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0234188.

Structurei

3D structure databases

ProteinModelPortaliQ54RD6.
SMRiQ54RD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0372. Eukaryota.
COG0639. LUCA.
InParanoidiQ54RD6.
KOiK17615.
OMAiFYTECVR.
PhylomeDBiQ54RD6.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54RD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSTFNLDH CIEKLQKCEI LPESTIKEIT DKMKELLISE SNVQEIRSPV
60 70 80 90 100
TVVGDVHGQF YDVLEIFKIG GQCPDTNYLF LGDYVDRGYH SVETISLLTC
110 120 130 140 150
LKLRYPSRIT LLRGNHESRQ ITQVYGFYGE CMRKYGNPTV WKYFTEMFDY
160 170 180 190 200
LSVAAIIDEA IYCVHGGLSP SALSIDQIKV LDRFQEVPNE GALSDILWSD
210 220 230 240 250
PDPDREGFVE SQRGAGYSYG KDVTLRFLQN NKMQHIIRAH QLCMDGYQTL
260 270 280 290 300
FDNKLSTVWS APNYCNRCGN MASIVEVNEK LERYFNTYAA APQSLSNKPT
310
LDTNKELPDY FL
Length:312
Mass (Da):35,694
Last modified:May 24, 2005 - v1
Checksum:i11DA221AB338021F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65832.1.
RefSeqiXP_639209.1. XM_634117.1.

Genome annotation databases

EnsemblProtistsiEAL65832; EAL65832; DDB_G0283187.
GeneIDi8623982.
KEGGiddi:DDB_G0283187.

Similar proteinsi

Entry informationi

Entry nameiPP2AB_DICDI
AccessioniPrimary (citable) accession number: Q54RD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 24, 2005
Last modified: September 27, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families