Q54RD6 (PP2AB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein phosphatase 2A catalytic subunit B
EC=3.1.3.16

Gene names

ORF Names:

DDB_G0283187

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core "AC" dimer equally active compared to the trimer By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Probable serine/threonine-protein phosphatase 2A catalytic subunit B		PRO_0000368207
Sites
	Active site	116	1	Proton donor By similarity
	Metal binding	55	1	Iron By similarity
	Metal binding	57	1	Iron By similarity
	Metal binding	83	1	Iron By similarity
	Metal binding	83	1	Manganese By similarity
	Metal binding	115	1	Manganese By similarity
	Metal binding	165	1	Manganese By similarity
	Metal binding	240	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54RD6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 11DA221AB338021F	FASTA	312	35,694
	10 20 30 40 50 60 MNTSTFNLDH CIEKLQKCEI LPESTIKEIT DKMKELLISE SNVQEIRSPV TVVGDVHGQF 70 80 90 100 110 120 YDVLEIFKIG GQCPDTNYLF LGDYVDRGYH SVETISLLTC LKLRYPSRIT LLRGNHESRQ 130 140 150 160 170 180 ITQVYGFYGE CMRKYGNPTV WKYFTEMFDY LSVAAIIDEA IYCVHGGLSP SALSIDQIKV 190 200 210 220 230 240 LDRFQEVPNE GALSDILWSD PDPDREGFVE SQRGAGYSYG KDVTLRFLQN NKMQHIIRAH 250 260 270 280 290 300 QLCMDGYQTL FDNKLSTVWS APNYCNRCGN MASIVEVNEK LERYFNTYAA APQSLSNKPT 310 LDTNKELPDY FL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000051 Genomic DNA. Translation: EAL65832.1.
RefSeq	XP_639209.1. XM_634117.1.
3D structure databases
HSSP	HSSP built from PDB template 1FJM based on UniProtKB P62139.
ProteinModelPortal	Q54RD6.
SMR	Q54RD6. Positions 6-311.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0185403.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0234188; DDB0234188; DDB_G0283187.
GeneID	8623982.
KEGG	ddi:DDB_G0283187.
Organism-specific databases
dictyBase	DDB_G0283187.
Phylogenomic databases
eggNOG	COG0639.
InParanoid	Q54RD6.
KO	K01090.
OMA	FREIPHE.
ProtClustDB	CLSZ2728979.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2AB_DICDI

Accession

Primary (citable) accession number: Q54RD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 24, 2005
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)