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Protein

Probable serine/threonine-protein phosphatase 2A catalytic subunit B

Gene

DDB_G0283187

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Manganese 1By similarity
Metal bindingi57 – 571Manganese 1By similarity
Metal bindingi83 – 831Manganese 1By similarity
Metal bindingi83 – 831Manganese 2By similarity
Metal bindingi115 – 1151Manganese 2By similarity
Active sitei116 – 1161Proton donorBy similarity
Metal bindingi165 – 1651Manganese 2By similarity
Metal bindingi240 – 2401Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable serine/threonine-protein phosphatase 2A catalytic subunit B (EC:3.1.3.16)
Gene namesi
ORF Names:DDB_G0283187
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283187.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Probable serine/threonine-protein phosphatase 2A catalytic subunit BPRO_0000368207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei312 – 3121Leucine methyl esterBy similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-312 by leucine carboxyl methyltransferase (LCMT) and protein phosphatase methylesterase 1 (ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity).By similarity

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core "AC" dimer equally active compared to the trimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi44689.DDB0234188.

Structurei

3D structure databases

ProteinModelPortaliQ54RD6.
SMRiQ54RD6. Positions 6-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ54RD6.
KOiK17615.
OMAiFYTECVR.
PhylomeDBiQ54RD6.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54RD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSTFNLDH CIEKLQKCEI LPESTIKEIT DKMKELLISE SNVQEIRSPV
60 70 80 90 100
TVVGDVHGQF YDVLEIFKIG GQCPDTNYLF LGDYVDRGYH SVETISLLTC
110 120 130 140 150
LKLRYPSRIT LLRGNHESRQ ITQVYGFYGE CMRKYGNPTV WKYFTEMFDY
160 170 180 190 200
LSVAAIIDEA IYCVHGGLSP SALSIDQIKV LDRFQEVPNE GALSDILWSD
210 220 230 240 250
PDPDREGFVE SQRGAGYSYG KDVTLRFLQN NKMQHIIRAH QLCMDGYQTL
260 270 280 290 300
FDNKLSTVWS APNYCNRCGN MASIVEVNEK LERYFNTYAA APQSLSNKPT
310
LDTNKELPDY FL
Length:312
Mass (Da):35,694
Last modified:May 24, 2005 - v1
Checksum:i11DA221AB338021F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65832.1.
RefSeqiXP_639209.1. XM_634117.1.

Genome annotation databases

EnsemblProtistsiDDB0234188; DDB0234188; DDB_G0283187.
GeneIDi8623982.
KEGGiddi:DDB_G0283187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65832.1.
RefSeqiXP_639209.1. XM_634117.1.

3D structure databases

ProteinModelPortaliQ54RD6.
SMRiQ54RD6. Positions 6-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0234188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0234188; DDB0234188; DDB_G0283187.
GeneIDi8623982.
KEGGiddi:DDB_G0283187.

Organism-specific databases

dictyBaseiDDB_G0283187.

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ54RD6.
KOiK17615.
OMAiFYTECVR.
PhylomeDBiQ54RD6.

Miscellaneous databases

PROiQ54RD6.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPP2AB_DICDI
AccessioniPrimary (citable) accession number: Q54RD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.