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Q54RD6 (PP2AB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable serine/threonine-protein phosphatase 2A catalytic subunit B

EC=3.1.3.16
Gene names
ORF Names:DDB_G0283187
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subunit structure

PP2A consists of a trimeric holoenzyme, composed of a 37 kDa catalytic subunit (C subunit) and a 65 kDa constant regulatory subunit (A subunit), that associates with a variety of regulatory subunits (B subunit) such as phr2AB (B55) and psrA (B56 homolog). The trimer may partially dissociates into a core "AC" dimer equally active compared to the trimer By similarity.

Post-translational modification

Reversibly methyl esterified on Leu-312 by leucine carboxyl methyltransferase (LCMT) and protein phosphatase methylesterase 1 (ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Probable serine/threonine-protein phosphatase 2A catalytic subunit B
PRO_0000368207

Sites

Active site1161Proton donor By similarity
Metal binding551Manganese 1 By similarity
Metal binding571Manganese 1 By similarity
Metal binding831Manganese 1 By similarity
Metal binding831Manganese 2 By similarity
Metal binding1151Manganese 2 By similarity
Metal binding1651Manganese 2 By similarity
Metal binding2401Manganese 2 By similarity

Amino acid modifications

Modified residue3121Leucine methyl ester By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54RD6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 11DA221AB338021F

FASTA31235,694
        10         20         30         40         50         60 
MNTSTFNLDH CIEKLQKCEI LPESTIKEIT DKMKELLISE SNVQEIRSPV TVVGDVHGQF 

        70         80         90        100        110        120 
YDVLEIFKIG GQCPDTNYLF LGDYVDRGYH SVETISLLTC LKLRYPSRIT LLRGNHESRQ 

       130        140        150        160        170        180 
ITQVYGFYGE CMRKYGNPTV WKYFTEMFDY LSVAAIIDEA IYCVHGGLSP SALSIDQIKV 

       190        200        210        220        230        240 
LDRFQEVPNE GALSDILWSD PDPDREGFVE SQRGAGYSYG KDVTLRFLQN NKMQHIIRAH 

       250        260        270        280        290        300 
QLCMDGYQTL FDNKLSTVWS APNYCNRCGN MASIVEVNEK LERYFNTYAA APQSLSNKPT 

       310 
LDTNKELPDY FL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000051 Genomic DNA. Translation: EAL65832.1.
RefSeqXP_639209.1. XM_634117.1.

3D structure databases

ProteinModelPortalQ54RD6.
SMRQ54RD6. Positions 6-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0185403.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0234188; DDB0234188; DDB_G0283187.
GeneID8623982.
KEGGddi:DDB_G0283187.

Organism-specific databases

dictyBaseDDB_G0283187.

Phylogenomic databases

eggNOGCOG0639.
InParanoidQ54RD6.
OMAFYTECVR.
PhylomeDBQ54RD6.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2AB_DICDI
AccessionPrimary (citable) accession number: Q54RD6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase