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Protein

DNA polymerase epsilon catalytic subunit A

Gene

pole

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase II participates in chromosomal DNA replication.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2225ZincBy similarity1
Metal bindingi2228ZincBy similarity1
Metal bindingi2285ZincBy similarity1
Metal bindingi2288ZincBy similarity1
Metal bindingi2319Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2322Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2334Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2337Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2225 – 2288CysA-typeAdd BLAST64

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-174430. Telomere C-strand synthesis initiation.
R-DDI-68952. DNA replication initiation.
R-DDI-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:pole
Synonyms:pol2
ORF Names:DDB_G0283189
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283189.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003283491 – 2380DNA polymerase epsilon catalytic subunit AAdd BLAST2380

Proteomic databases

PaxDbiQ54RD4.
PRIDEiQ54RD4.

Interactioni

Subunit structurei

Consists of three subunits: pole, pole2 and pole3.Curated

Protein-protein interaction databases

STRINGi44689.DDB0216320.

Structurei

3D structure databases

SMRiQ54RD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2319 – 2337CysB motifAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi174 – 179Poly-Asn6
Compositional biasi182 – 195Poly-AsnAdd BLAST14
Compositional biasi203 – 207Poly-Asn5
Compositional biasi1323 – 1327Poly-Ser5
Compositional biasi1966 – 1973Poly-Gln8
Compositional biasi1977 – 1983Poly-Asp7
Compositional biasi2026 – 2029Poly-Ile4
Compositional biasi2064 – 2067Poly-Ser4
Compositional biasi2075 – 2079Poly-Thr5
Compositional biasi2110 – 2114Poly-Ser5
Compositional biasi2115 – 2119Poly-Thr5

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C.By similarity
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2225 – 2288CysA-typeAdd BLAST64

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1798. Eukaryota.
COG0417. LUCA.
InParanoidiQ54RD4.
KOiK02324.
OMAiIHSKEIF.
PhylomeDBiQ54RD4.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiView protein in InterPro
IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR023211. DNA_pol_palm_dom_sf.
IPR029703. POL2.
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
PANTHERiPTHR10670. PTHR10670. 1 hit.
PfamiView protein in Pfam
PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
SMARTiView protein in SMART
SM01159. DUF1744. 1 hit.
SM00486. POLBc. 1 hit.
SUPFAMiSSF53098. SSF53098. 2 hits.

Sequencei

Sequence statusi: Complete.

Q54RD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKWNENKK NKDEKDENDK YVGFKNYEKE GWLLNMQPGS SKDGLDIEKA
60 70 80 90 100
SLELFFIQDD ATSFRVWIPY NPYFYIYVKE GHQSEVESYL KSTYEKDIAG
110 120 130 140 150
IDIMDKEDLD LENHLSGLKR KYLKIRFHNV STLLSVRNDL FPIIKRNKQK
160 170 180 190 200
SNTSEAYEDE SLNKILNSYY NKGNNNNNNH QNYNNNNNQN NNNFNKELNK
210 220 230 240 250
DDNNNNNSKL NKQATEYILD IREYDVPYYV RAAIDLNIRV GLWYTVIKNG
260 270 280 290 300
RLTTVNELTT RVDRPDPKVL AYDIETTKLP LKFPDSSIDS IMMISYMLDK
310 320 330 340 350
QGYLIVNREI VSEDIKDFEY TPKPEYYGPF TVFNEPDEKS VLERFFSEIK
360 370 380 390 400
REKPHIFVSY NGDMFDWPFV ESRAEYHGLS MFHQIGFRND NGEYRSKINP
410 420 430 440 450
HMDAFCWVKR DSYLPHGSHG LKAVTREKLR YDPLELDPEL MLKSAQEDPE
460 470 480 490 500
TLANYSVSDA VATYYLYMNY VHPFIFSLCT IIPMNPDDVL RKGSGTLCEA
510 520 530 540 550
LLMTQAFKAD VIFPNKHKDD INSMYKGHLL ESETYVGGHV ECLESGVFRS
560 570 580 590 600
DIPTNFSLDP ASIEKHMGNI DQVLKFALKE GGIPLDTVSN YQEVKEDVLK
610 620 630 640 650
KFTLLKENPK QQSHPLIYHL DVSAMYPNII LTNKLQPTAV VNDEVCATCV
660 670 680 690 700
YNKPESQCQR TLDWQWRGDY SPSNQSEYRL ILQQLESEKF GDGDERKSFL
710 720 730 740 750
SLSEEKRNEL LRKRLKEYSR KVYRKTHQIT QEIRSDTICM RENSFYVDTV
760 770 780 790 800
RLFRDRRYVF KNHHRDWKIK YDQAMQESGG TNSVAVVAAQ GMVVLYESLQ
810 820 830 840 850
LAHKCILNSF YGYVMRKGAR WYSMQMAGIV THTGSNIIKE AREVVEQMGR
860 870 880 890 900
PLEIDTDGIW CILPSHFPEN YTLKSASTGK KVTFSYICEM LNEKVAKSFT
910 920 930 940 950
NHQYQDYNAE TNTYTIRDEC SILFECDGPY RCMLIPTSKE KDVKLKKRYA
960 970 980 990 1000
VFNREGRICE LKGFEIKRRG ELKLIKLFQS EVFKEFLGGD SLEGCYQSVG
1010 1020 1030 1040 1050
AVANRWLDIL DSHAENYEEK DLIELITESS NMSRKLEEYG TQKSSAISTA
1060 1070 1080 1090 1100
KKLAEFLGDD MIKDKGLSCQ YIISNKPAGS PITERALPVA IFDADFETRC
1110 1120 1130 1140 1150
HYLRRWTKSP SGDLDIRELI DWDYYRQRLS GVIQKIITIP AALQNVTNPV
1160 1170 1180 1190 1200
PRVIHPDWIL KEIRRNEDGR QQTSITSFFE KTEDNDQDND NDNDNDNDND
1210 1220 1230 1240 1250
NDNSKPQQTD DIENMFSSRF KDLPAFNPKV TKFKRSKQSS SDVSSLLIKK
1260 1270 1280 1290 1300
SKSSIETFTN EQEKQIMETK TPSIDKDFQG WLSISKKKWK IQRLLKKRRQ
1310 1320 1330 1340 1350
RLGGSLFDSD SFRIGSKLTN RESSSSSFFK SQSDIIKKGY WNIISIEPIY
1360 1370 1380 1390 1400
GGEPGIYQMW SLIEDQLIPI KVDIGRVFYL NSIDTDPYED AQKNTNVIPP
1410 1420 1430 1440 1450
RGKQRFNVFS VTMSESQFLE QSKELNTLFT NPMIEGVYET KVPLDIKAII
1460 1470 1480 1490 1500
QCGCVATLSR VSPFFTKIVN SNTRFSLEDI ASKPDKQFHY LAEHNFNQLF
1510 1520 1530 1540 1550
LYHNSRDGKD GYFVLFNMNT QQCSVIFSNP YMAANKIDAR VLNSIKEKLP
1560 1570 1580 1590 1600
EITFTMDQKS SMSLAKKEIG SLIMEYQRKG LPTIILLQVP NAIGILEQIP
1610 1620 1630 1640 1650
ILREFPRVPV PYHDNDSMYS PFNWDVHSLK PLPLRLMDAP KLWVYYANMS
1660 1670 1680 1690 1700
RYANIPIGNI PTDNASFMCD ILYARSLVEQ KHLLWMSDSN FPDLGGSEED
1710 1720 1730 1740 1750
DAKFYEELNN VEINNSDCYN QVCFELDIEN LATNTILESI HLAEIEGILG
1760 1770 1780 1790 1800
NELGDESNVA MFDDIKNTSN NSNTKNGANQ NTTNDTTSSF DKLSITNKFR
1810 1820 1830 1840 1850
SAINHQISGC EKEFNILRNL VSKWKLDLVS GSNSKRNEIS SRYSNYLLLH
1860 1870 1880 1890 1900
FYRWISSTNS KLYDPILYRT LHQLMKKVFI QLIFEFKKLG SKIVYANFNK
1910 1920 1930 1940 1950
IIICSQKDSI EDARSYCNYI LAVIKKKELF SWINFKQTNY YHNLLWLNNS
1960 1970 1980 1990 2000
NYSGILYFNP LINKNQQQQQ QQQDNADDDD DDDVSENEEE QQQNKNKKLK
2010 2020 2030 2040 2050
KIKNGKIITN WNIAEFLPPQ IQTSFIIIIS DYIYKLHTER DELNRIQKEQ
2060 2070 2080 2090 2100
QQRYLLKQKI STTSSSSNND STAATTTTTK DTINEPTKPD LRKSSTWNEE
2110 2120 2130 2140 2150
EDEDISIPPS SSSSTTTTTS KTKKATSKFD LLIDVNRIFT LLDHFQVNQS
2160 2170 2180 2190 2200
SLEFPQLPGG YLKLNNPPLE FIKFVCHVLS IDKSISSRVS RLRMKLMTSM
2210 2220 2230 2240 2250
KVREFSDEAK FKDPCVSFTL PDVICSSCHS CRDIDLLRNT NTTSISSRLS
2260 2270 2280 2290 2300
SQQKSNNNDS DDSDDDNEEN EGDDDNIIRV PELSCIQCKG HYSKNTIESQ
2310 2320 2330 2340 2350
LVEIIQRRSL SYQLQDLRCS KCNDVKSDNL GDICPQCSGQ WECTQSNNLF
2360 2370 2380
SKDLIIFKSI AEYHNFEWLG ETVDSLSKFI
Length:2,380
Mass (Da):275,442
Last modified:May 24, 2005 - v1
Checksum:iB4DC5FAA0542994B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65833.1.
RefSeqiXP_639211.1. XM_634119.1.

Genome annotation databases

EnsemblProtistsiEAL65833; EAL65833; DDB_G0283189.
GeneIDi8623984.
KEGGiddi:DDB_G0283189.

Similar proteinsi

Entry informationi

Entry nameiDPOE1_DICDI
AccessioniPrimary (citable) accession number: Q54RD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 22, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families