SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54RC3

- TOP1_DICDI

UniProt

Q54RC3 - TOP1_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA topoisomerase 1
Gene
top1, DDB_G0283205
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei409 – 4091Interaction with DNA By similarity
Sitei457 – 4571Interaction with DNA By similarity
Sitei506 – 5061Interaction with DNA By similarity
Sitei537 – 5371Interaction with DNA By similarity
Sitei594 – 5941Interaction with DNA By similarity
Sitei620 – 6201Interaction with DNA By similarity
Sitei662 – 6621Interaction with DNA By similarity
Sitei721 – 7211Interaction with DNA By similarity
Sitei739 – 7391Interaction with DNA By similarity
Active sitei853 – 8531O-(3'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: dictyBase
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: dictyBase
  2. DNA topological change Source: dictyBase
  3. chromatin remodeling Source: RefGenome
  4. chromosome segregation Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:top1
ORF Names:DDB_G0283205
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283205. top1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleolus Source: RefGenome
  2. nucleus Source: dictyBase
  3. replication fork protection complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893DNA topoisomerase 1
PRO_0000330481Add
BLAST

Proteomic databases

PRIDEiQ54RC3.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0185416.

Structurei

3D structure databases

ProteinModelPortaliQ54RC3.
SMRiQ54RC3. Positions 847-885.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni519 – 5202Interaction with DNA By similarity
Regioni582 – 5876Interaction with DNA By similarity
Regioni673 – 6753Interaction with DNA By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 316Poly-Thr
Compositional biasi92 – 202111Ser-rich
Add
BLAST
Compositional biasi144 – 16522Thr-rich
Add
BLAST
Compositional biasi223 – 27755Thr-rich
Add
BLAST
Compositional biasi284 – 29411Poly-Glu
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3569.
KOiK03163.
OMAiREKFNWA.
PhylomeDBiQ54RC3.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54RC3-1 [UniParc]FASTAAdd to Basket

« Hide

MEVKQEILTP MKVEKTNGNV PTPPPTTTTT TVMDDDLFDD EASPPQPNKK    50
LKAENGSIKK LTPTPKPAPV TPKPTAAVNG KKPIPKKKPV DSSSDSDSSD 100
SSSDSDSDSS DSDSDSSSSD SSSSDSDVPK KKPISKIPNK PSGTVSKTSK 150
NSPKTTTTTT TTTTTVKSKP APKVKPEQSS SDDSSSDSSD DSSDDSSSDS 200
SSEEEKPKKK PSKKPAAVKK EITVKKETTT TTKKTPVKKE VEVKKETKTT 250
TTKKVKKEES DDDGEDEKTT TKKKTTTRKR KLKEEEEGEE EEEEGVNKWW 300
DKEDDEDDGT KWTTLIHNGP VFPPAYVPHG IKFMYDGKVV KLTPKQEEVA 350
TFYANYLETD HVKKQAFKDN FFKEFKGLLT SEQKEIVKKM DKCDFSHIHT 400
HLKTKKEQRK ARSKEEKDAE TAEKKKIQEK HGFATIDGFK EKVGNYMIEP 450
PGLFLGRGQH PKTGMLKQRI MPSDVTLNIG EGEVIPPSPI PGEKWKEIVH 500
NNTVTWLAFW RESVNGGFKY VWLAPSSKIK GQADRKKFEV ARGLKNCIGD 550
IRKGYQKSFN DESTEKRQLA VALYLIDFLA LRVGNEKGED QADTVGCCSL 600
RVEHIKLESN NTITLDFLGK DSMRYLNTVQ IREDAYKVLK TFVKGKKPTD 650
LLFDSLTVTQ LNNHLKKQMT GLSAKVFRTY NASITLQKEL DKMDTKEFQS 700
IDEKILFYNR CNREVAILCN HQKAPPKNLA ETMSKIDEKI QELRDLQDLC 750
RIKIGKTPKS QQEDIESREA ERLPKLIQLA ITNKKDKDKE KTDKEISFDV 800
KAAFEKSRTL PDAKGKIKEK IAKYEEQIKK QEILKTGKDE LKTVALGTSK 850
INYLDPRITV AWCKKNKVPV DKIFTKSLRD KFPWSDVGTD YIF 893
Length:893
Mass (Da):100,612
Last modified:May 24, 2005 - v1
Checksum:iDD9BB5F99BF3331E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65841.1.
RefSeqiXP_639222.1. XM_634130.1.

Genome annotation databases

EnsemblProtistsiDDB0234191; DDB0234191; DDB_G0283205.
GeneIDi8623995.
KEGGiddi:DDB_G0283205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000051 Genomic DNA. Translation: EAL65841.1 .
RefSeqi XP_639222.1. XM_634130.1.

3D structure databases

ProteinModelPortali Q54RC3.
SMRi Q54RC3. Positions 847-885.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0185416.

Proteomic databases

PRIDEi Q54RC3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0234191 ; DDB0234191 ; DDB_G0283205 .
GeneIDi 8623995.
KEGGi ddi:DDB_G0283205.

Organism-specific databases

dictyBasei DDB_G0283205. top1.

Phylogenomic databases

eggNOGi COG3569.
KOi K03163.
OMAi REKFNWA.
PhylomeDBi Q54RC3.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTOP1_DICDI
AccessioniPrimary (citable) accession number: Q54RC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi