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Protein

Mitogen-activated protein kinase kinase kinase A

Gene

mkkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates cell-type differentiation and spatial patterning, required for the proper induction and maintenance of prespore cell differentiation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199ATPPROSITE-ProRule annotationBy similarity1
Active sitei297Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi176 – 184ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: GO_Central
  • anatomical structure morphogenesis Source: dictyBase
  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sporulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase A1 Publication (EC:2.7.11.25)
Alternative name(s):
MAPK/ERK kinase 1
Short name:
MEK kinase 1Imported
Short name:
MEKK 1Imported
MAPK/ERK kinase A
Short name:
MEK kinase A1 Publication
Short name:
MEKK A1 Publication
Short name:
MEKKalpha1 Publication
Gene namesi
Name:mkkAImported
ORF Names:DDB_G0283265
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283265. mkkA.

Subcellular locationi

  • Membrane Sequence analysis; Single-pass membrane protein Sequence analysis

  • Note: Localized predominantly at the periphery of the cells, possibly in the cortex or the plasma membrane.Sequence analysis1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei513 – 533HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cell cortex Source: dictyBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Cells develop precociously and exhibit abnormal cell-type patterning with an increase in the pstO prestalk compartment, a concomitant reduction in the prespore domain, and a loss of the sharp compartment boundaries, resulting in overlapping prestalk and prespore domains.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003712501 – 942Mitogen-activated protein kinase kinase kinase AAdd BLAST942

Post-translational modificationi

ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation in a cell-type-specific and temporally regulated manner.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ54R82.

Interactioni

Subunit structurei

Interacts with ubcB and ubpB.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0220118.

Structurei

3D structure databases

ProteinModelPortaliQ54R82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 96PB1PROSITE-ProRule annotationAdd BLAST82
Domaini170 – 429Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini518 – 564F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati607 – 646WD 1Sequence analysisAdd BLAST40
Repeati690 – 733WD 2Sequence analysisAdd BLAST44
Repeati736 – 778WD 3Sequence analysisAdd BLAST43
Repeati780 – 825WD 4Sequence analysisAdd BLAST46
Repeati828 – 865WD 5Sequence analysisAdd BLAST38
Repeati872 – 909WD 6Sequence analysisAdd BLAST38
Repeati912 – 942WD 7Sequence analysisAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 144Poly-AsnSequence analysisAdd BLAST24
Compositional biasi449 – 461Poly-ThrSequence analysisAdd BLAST13
Compositional biasi471 – 479Poly-GlnSequence analysis9
Compositional biasi504 – 513Poly-SerSequence analysis10

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
InParanoidiQ54R82.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000270. PB1_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00666. PB1. 1 hit.
SM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS51745. PB1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54R82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWLNNPSQN FVDPFIRIKC ILGDDIRIIK FNSNISYGGL MNQLEQDFQC
60 70 80 90 100
PISIHQYEDY EGDKVTVKSK DDIMEALTMY FELKALNPTK IISTKFFLKQ
110 120 130 140 150
LPPQSQPLSS SLSPTQSLIL NNNNNNNNNN NNNNNNNNNN NNNNIIQHTD
160 170 180 190 200
FPSLIINEHE ELISNHNIKW QKGQILGRGG YGSVYLGLNK DTGELFAVKQ
210 220 230 240 250
LEIVDINSDP KLKNMILSFS KEIEVMRSLR HDNIVRYLGT SLDQSFLSVF
260 270 280 290 300
LEYIPGGSIS SLLGKFGAFS ENVIKVYTKQ ILQGLSFLHA NSIIHRDIKG
310 320 330 340 350
ANILIDTKGI VKLSDFGCSK SFSGIVSQFK SMQGTPYWMA PEVIKQTGHG
360 370 380 390 400
RSSDIWSLGC VIVEMATAQP PWSNITELAA VMYHIASSNS IPNIPSHMSQ
410 420 430 440 450
EAFDFLNLCF KRDPKERPDA NQLLKHPFIM NLDDNIQLPT ISPTTTLSTN
460 470 480 490 500
TTNTTATTTT TNNATNSNIN QQQQQQQQQP PTRTQRVSIS AGSSNNKRYT
510 520 530 540 550
PPISTSTSSS SSSILNNFSI NIILPINLII LIFREIKPNF VNTLSRVCKH
560 570 580 590 600
WKQIIDDDEL WNKYCSDRLI NKSKFEESIT WKSNYIKIYK QQKVWFHNKL
610 620 630 640 650
NHSTLKGHDK GVFCVKLIDD QGMVLSGGED KKLKVWDISG NHHHNHHSGI
660 670 680 690 700
VGSISKKSGL NIINNNNSNN SNSSSNSSSS NSRYLFSLKG HSGCIKSVDY
710 720 730 740 750
QRQSGSDVSR VFTASADFTC KIFSLKTKKT LFTYTNHQEA VTCINYLGDV
760 770 780 790 800
ENKCITSSLD KTIQLWDAET GSCLSTLRGH TGGIYCVKTD QVATHGNGYN
810 820 830 840 850
HLVVSASVDK TSNVWDTRSS SKVRSFTQHT EDVLCCYVFD QKVVTGSCDG
860 870 880 890 900
TIKLWDIGTG KTISTFIPSE TRQKNYVWTV QFDQSKIISS GKTGIIRIWD
910 920 930 940
IYNERDSRSI GGHHETIFSL QFNNQKLITG SLDKLVKIWS ID
Length:942
Mass (Da):105,796
Last modified:May 5, 2009 - v2
Checksum:iC9E4928A8C7C68F7
GO

Sequence cautioni

The sequence EAL65773 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093689 mRNA. Translation: AAC97114.1.
AAFI02000052 Genomic DNA. Translation: EAL65773.2. Sequence problems.
RefSeqiXP_639165.2. XM_634073.2.

Genome annotation databases

EnsemblProtistsiEAL65773; EAL65773; DDB_G0283265.
GeneIDi8624035.
KEGGiddi:DDB_G0283265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093689 mRNA. Translation: AAC97114.1.
AAFI02000052 Genomic DNA. Translation: EAL65773.2. Sequence problems.
RefSeqiXP_639165.2. XM_634073.2.

3D structure databases

ProteinModelPortaliQ54R82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0220118.

Proteomic databases

PaxDbiQ54R82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65773; EAL65773; DDB_G0283265.
GeneIDi8624035.
KEGGiddi:DDB_G0283265.

Organism-specific databases

dictyBaseiDDB_G0283265. mkkA.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
InParanoidiQ54R82.

Miscellaneous databases

PROiQ54R82.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000270. PB1_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00666. PB1. 1 hit.
SM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS51745. PB1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMKKA_DICDI
AccessioniPrimary (citable) accession number: Q54R82
Secondary accession number(s): O96611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.