Reviewed, UniProtKB/Swiss-Prot Q54R82 (MKKA_DICDI)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase A
    EC=2.7.11.25
Alternative name(s):
    MAPK/ERK kinase 1
      Short name=MEK kinase 1
      Short name=MEKK 1
    MAPK/ERK kinase A
      Short name=MEK kinase A
      Short name=MEKK A
      Short name=MEKKalpha

Gene names

Name:	mkkA
ORF Names:	DDB_G0283265

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates cell-type differentiation and spatial patterning, required for the proper induction and maintenance of prespore cell differentiation. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q869N2
Cofactor	Magnesium By similarity. UniProtKB Q869N2
Subunit structure	Interacts with ubcB and ubpB. Ref.1
Subcellular location	Membrane; Single-pass membrane protein Potential. Note: Localized predominantly at the periphery of the cells, possibly in the cortex or the plasma membrane. Ref.1
Post-translational modification	ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation in a cell-type-specific and temporally regulated manner.
Disruption phenotype	Cells develop precociously and exhibit abnormal cell-type patterning with an increase in the pstO prestalk compartment, a concomitant reduction in the prespore domain, and a loss of the sharp compartment boundaries, resulting in overlapping prestalk and prespore domains. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 F-box domain. Contains 1 OPR domain. Contains 1 protein kinase domain. Contains 7 WD repeats.
Sequence caution	The sequence EAL65773.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Membrane
Domain	Repeat Transmembrane WD repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro sorocarp development Ref.1 Inferred from mutant phenotype. Source: dictyBase sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell cortex Ref.1 Inferred from direct assay. Source: dictyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 942	942	Mitogen-activated protein kinase kinase kinase A		PRO_0000371250
Regions
	Transmembrane	513 – 533	21	Potential
	Domain	15 – 101	87	OPR
	Domain	170 – 429	260	Protein kinase
	Domain	518 – 564	47	F-box
	Repeat	607 – 646	40	WD 1
	Repeat	690 – 733	44	WD 2
	Repeat	736 – 778	43	WD 3
	Repeat	780 – 825	46	WD 4
	Repeat	828 – 865	38	WD 5
	Repeat	872 – 909	38	WD 6
	Repeat	912 – 942	31	WD 7
	Nucleotide binding	176 – 184	9	ATP By similarity UniProtKB P28523
	Compositional bias	121 – 144	24	Poly-Asn
	Compositional bias	449 – 461	13	Poly-Thr
	Compositional bias	471 – 479	9	Poly-Gln
	Compositional bias	504 – 513	10	Poly-Ser
Sites
	Active site	297	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	199	1	ATP By similarity UniProtKB P28523

Sequences

	Sequence		Length	Mass (Da)
	Q54R82-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: C9E4928A8C7C68F7	FASTA	942	105,796
	10 20 30 40 50 60 MSWLNNPSQN FVDPFIRIKC ILGDDIRIIK FNSNISYGGL MNQLEQDFQC PISIHQYEDY 70 80 90 100 110 120 EGDKVTVKSK DDIMEALTMY FELKALNPTK IISTKFFLKQ LPPQSQPLSS SLSPTQSLIL 130 140 150 160 170 180 NNNNNNNNNN NNNNNNNNNN NNNNIIQHTD FPSLIINEHE ELISNHNIKW QKGQILGRGG 190 200 210 220 230 240 YGSVYLGLNK DTGELFAVKQ LEIVDINSDP KLKNMILSFS KEIEVMRSLR HDNIVRYLGT 250 260 270 280 290 300 SLDQSFLSVF LEYIPGGSIS SLLGKFGAFS ENVIKVYTKQ ILQGLSFLHA NSIIHRDIKG 310 320 330 340 350 360 ANILIDTKGI VKLSDFGCSK SFSGIVSQFK SMQGTPYWMA PEVIKQTGHG RSSDIWSLGC 370 380 390 400 410 420 VIVEMATAQP PWSNITELAA VMYHIASSNS IPNIPSHMSQ EAFDFLNLCF KRDPKERPDA 430 440 450 460 470 480 NQLLKHPFIM NLDDNIQLPT ISPTTTLSTN TTNTTATTTT TNNATNSNIN QQQQQQQQQP 490 500 510 520 530 540 PTRTQRVSIS AGSSNNKRYT PPISTSTSSS SSSILNNFSI NIILPINLII LIFREIKPNF 550 560 570 580 590 600 VNTLSRVCKH WKQIIDDDEL WNKYCSDRLI NKSKFEESIT WKSNYIKIYK QQKVWFHNKL 610 620 630 640 650 660 NHSTLKGHDK GVFCVKLIDD QGMVLSGGED KKLKVWDISG NHHHNHHSGI VGSISKKSGL 670 680 690 700 710 720 NIINNNNSNN SNSSSNSSSS NSRYLFSLKG HSGCIKSVDY QRQSGSDVSR VFTASADFTC 730 740 750 760 770 780 KIFSLKTKKT LFTYTNHQEA VTCINYLGDV ENKCITSSLD KTIQLWDAET GSCLSTLRGH 790 800 810 820 830 840 TGGIYCVKTD QVATHGNGYN HLVVSASVDK TSNVWDTRSS SKVRSFTQHT EDVLCCYVFD 850 860 870 880 890 900 QKVVTGSCDG TIKLWDIGTG KTISTFIPSE TRQKNYVWTV QFDQSKIISS GKTGIIRIWD 910 920 930 940 IYNERDSRSI GGHHETIFSL QFNNQKLITG SLDKLVKIWS ID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation." Chung C.Y., Reddy T.B.K., Zhou K., Firtel R.A. Genes Dev. 12:3564-3578(1998) [PubMed: 9832508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH UBC AND UPC, SUBCELLULAR LOCATION, UBIQUITINATION, DISRUPTION PHENOTYPE. Strain: AX3-1.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093689 mRNA. Translation: AAC97114.1. AAFI02000052 Genomic DNA. Translation: EAL65773.2. Sequence problems.
RefSeq	XP_639165.2.
3D structure databases
HSSP	HSSP built from PDB template 2CLQ based on UniProtKB Q99683.
SMR	Q54R82. Positions 170-429.
ModBase	Search...
Genome annotation databases
GeneID	8624035.
KEGG	ddi:DDB_0220118.
Organism-specific databases
dictyBase	DDB_G0283265. mkkA.
Phylogenomic databases
eggNOG	KOG0274.
InParanoid	Q54R82.
PhylomeDB	Q54R82.
Family and domain databases
InterPro	IPR001810. F-box. IPR020472. G-protein_beta_WD-40_rep_reg. IPR011009. Kinase-like_dom. IPR015748. MAPKKK3. IPR000270. OPR_PB1. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PANTHER	PTHR22986:SF64. MAPKKK3. 1 hit.
Pfam	PF00646. F-box. 1 hit. PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00256. FBOX. 1 hit. SM00666. PB1. 1 hit. SM00220. S_TKc. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MKKA_DICDI

Accession

Primary (citable) accession number: Q54R82
Secondary accession number(s): O96611

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	February 9, 2010
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)