Q54QV3 (YAKA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase yakA
EC=2.7.12.1

Gene names

Name:	yakA
Synonyms:	dagB
ORF Names:	DDB_G0283605

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General sensor of environmental conditions, such as heat stress, effecting changes through pkaC. Essential for survival to nitrosoative and oxidative stresses. Required for cell cycle control, not only at the onset but also during development (aggregation process and postaggregative development). Ref.2 Ref.3 Ref.4 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Ref.4.
Developmental stage	Expressed during growth-to-development stages with increase in cell density. Ref.2
Disruption phenotype	Null cells fail to enter development, express low levels of cAMP receptors, are smaller than wild-type cells, are hypersensitive to nitrosoative/oxidative stress, and display slow growth on bacterial lawns. Ref.3 Ref.4 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aggregation involved in sorocarp development Traceable author statement. Source: dictyBase chemotaxis Traceable author statement. Source: dictyBase signal transduction Traceable author statement. Source: dictyBase
Cellular component	cytosol Traceable author statement. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1458	1458	Probable serine/threonine-protein kinase yakA		PRO_0000362075
Regions
	Domain	205 – 548	344	Protein kinase
	Nucleotide binding	211 – 219	9	ATP By similarity
	Coiled coil	588 – 643	56	Potential
	Coiled coil	878 – 927	50	Potential
	Coiled coil	1346 – 1383	38	Potential
	Coiled coil	1409 – 1442	34	Potential
	Compositional bias	14 – 17	4	Poly-Thr
	Compositional bias	45 – 76	32	Poly-Asn
	Compositional bias	451 – 459	9	Poly-Asn
	Compositional bias	594 – 626	33	Poly-Gln
	Compositional bias	629 – 642	14	Poly-Gln
	Compositional bias	692 – 697	6	Poly-Gln
	Compositional bias	729 – 733	5	Poly-Gln
	Compositional bias	813 – 816	4	Poly-Gln
	Compositional bias	819 – 853	35	Poly-Asn
	Compositional bias	881 – 922	42	Poly-Gln
	Compositional bias	967 – 988	22	Poly-Gln
	Compositional bias	1016 – 1022	7	Poly-Gln
	Compositional bias	1033 – 1038	6	Poly-Gln
	Compositional bias	1073 – 1086	14	Poly-Gln
	Compositional bias	1200 – 1207	8	Poly-Gln
	Compositional bias	1256 – 1262	7	Poly-Gly
	Compositional bias	1263 – 1269	7	Poly-Asn
	Compositional bias	1346 – 1378	33	Poly-Gln
	Compositional bias	1411 – 1441	31	Poly-Gln
Sites
	Active site	332	1	Proton acceptor By similarity
	Binding site	234	1	ATP By similarity
Experimental info
	Mutagenesis	375	1	P → S: Causes a temperature-sensitive phenotype, which fails to develop at restrictive temperature. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q54QV3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 656073216ADF46EF	FASTA	1,458	166,973
	10 20 30 40 50 60 MGSTTQMSDY LVSTTTTSSA SSMVTMTSQI ENNSLNSNDN TNTTNNNNNN NNNNNNNNNN 70 80 90 100 110 120 NNNNNNNNNI NNNNNNGGMV GVNHKTHKKT ASIGGGKNRA ILHDFTFSYF IPNQNRSEDN 130 140 150 160 170 180 SNENELGLNN HNVQHQGPII HLTKNLLHFY KKCNSNFNYI SSLNPRRVLT HPSEPLSNDG 190 200 210 220 230 240 YDNVNSDYIV YVNDIITNNE SGQKYKVLDS LGQGTFGQVV KCKNCDTDEL VAIKILKNKQ 250 260 270 280 290 300 AYFQQGRLEI QTLKSLNDQH DPEDKNHILR LLDSFIHKMH LCIVFELLSV NLFELIKQNN 310 320 330 340 350 360 FRGLSTNLIK VFLIQILDAL IVLANANIIH CDLKPENILL QNVNSPAIKI IDFGSACYEK 370 380 390 400 410 420 STLYTYIQSR HYRSPEVLVG TVYCASIDMW SLGCISAELF LGLPLFPGNS EYNQISRIVE 430 440 450 460 470 480 MRGIFPSDLL DKGKSSTRYF HRHLGSNSDD NNNNNNNNNG KPYYYTLKSE EDYQRDSKTT 490 500 510 520 530 540 LLPSKKYFNY KTLPEIIQNY GFKKSMSPQD IEKEKQHRIV FTDFINGLLQ LDPNERWSPM 550 560 570 580 590 600 QAKEHPFITG QPYNGPFIPD PSKKRHFTYS QPKQIPQHSM LNGNQILNQH QLFQQLQQQQ 610 620 630 640 650 660 QQQQQQQQQQ QQQQQQQQQQ QQQQQQHNQF QQQQQQQQQQ QQSSSQQHIQ IQPLQLFSTP 670 680 690 700 710 720 YTSTNNTPNL SSSNSGSSLN NLKKLNLPPF KQQQQQQFST SQNSDSFNFP NESFSPRGIY 730 740 750 760 770 780 IPSSASNIQQ QQQPININNN QNGVGSQVSQ LALGQSPSLF GTPTNIYPPY SSMYNNSPVA 790 800 810 820 830 840 TPNSLSFYGS SWGSDSSSIS LNPSTPTQKQ MFQQQQYSNN NNNNNNNNNN NSNNNNGNNT 850 860 870 880 890 900 NNINSNNNNN NVNRRNRSKS DIPSDSFSSS EGMDPQFNLY QQQQQQQQQQ QQQQQQQQQQ 910 920 930 940 950 960 QQQQQQQQQQ QQQQLQYQQQ FQTLQDLNIE GEKPPIYPNS PHRKRSHSGY LDQYANGYNS 970 980 990 1000 1010 1020 QQTNNTQQQQ QQQQQQQQQQ QQQQQQQQHP SFFSTRMHLD HFNGGSRYRS QSYGDQQFQQ 1030 1040 1050 1060 1070 1080 QQYKQQQKNL HHQQQQQQRF MQVGSPPTSH LSPPIPQSPL MMSQPLHQTY IPQQQQQQQQ 1090 1100 1110 1120 1130 1140 QQQQSQPTPF TPQMISQEPI SPALMGDASS IWNPSPTEEL LFTIDLPNQQ NTPHLTPSNS 1150 1160 1170 1180 1190 1200 STNLLGKSAS SPLKNSSGGA IPPTPTIPIN MEEINNGFSK FHFNDQPSWN SNGNSPWMIQ 1210 1220 1230 1240 1250 1260 QQQQHQQGFN GNSESMYNDD LIGFSPYNNY SNDYRPQLFN KQSPPSSYNS NKSFYGGSGG 1270 1280 1290 1300 1310 1320 GGNNNNNNNS RPTNQNFSNS LLPSQQQNVI FPQNSPPSSY NSSNSLSKSG GNTVKNNSNT 1330 1340 1350 1360 1370 1380 GGRPRGDSMK QRFNSTNNLL SGGSYQYQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYK 1390 1400 1410 1420 1430 1440 KDTPSLMMSP PMNGLKTSSE IGIPSEEIRY QYQQQQLQQQ FQQQQQQQQQ QQIQQQLQQQ 1450 QAPPQNRKQV VIGSYRET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"YakA, a protein kinase required for the transition from growth to development in Dictyostelium." Souza G.M., Lu S., Kuspa A. Development 125:2291-2302(1998) [PubMed: 9584128] [Abstract] Cited for: DEVELOPMENTAL STAGE, FUNCTION.
[3]	"Starvation promotes Dictyostelium development by relieving PufA inhibition of PKA translation through the YakA kinase pathway." Souza G.M., da Silva A.M., Kuspa A. Development 126:3263-3274(1999) [PubMed: 10375515] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]	"The protein kinase YakA regulates G-protein-linked signaling responses during growth and development of Dictyostelium." van Es S., Weening K.E., Devreotes P.N. J. Biol. Chem. 276:30761-30765(2001) [PubMed: 11410593] [Abstract] Cited for: MUTAGENESIS OF PRO-375, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, FUNCTION.
[5]	"Role for YakA, cAMP, and protein kinase A in regulation of stress responses of Dictyostelium discoideum cells." Taminato A., Bagattini R., Gorjao R., Chen G., Kuspa A., Souza G.M. Mol. Biol. Cell 13:2266-2275(2002) [PubMed: 12134067] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000056 Genomic DNA. Translation: EAL65569.1.
RefSeq	XP_638920.1. XM_633828.1.
3D structure databases
HSSP	HSSP built from PDB template 1HOW based on UniProtKB Q03656.
ProteinModelPortal	Q54QV3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191191; DDB0191191; DDB_G0283605.
GeneID	8624159.
GenomeReviews	Gene locus yakA in contig CM000153_GR.
KEGG	ddi:DDB_G0283605.
Organism-specific databases
dictyBase	DDB_G0283605. yakA.
Phylogenomic databases
GeneTree	EPrGT00060000009507.
InParanoid	Q54QV3.
PhylomeDB	Q54QV3.
ProtClustDB	CLSZ2846739.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAKA_DICDI

Accession

Primary (citable) accession number: Q54QV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 24, 2005
Last modified:	October 19, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)