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Q54QQ0 (IMDH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inosine-5'-monophosphate dehydrogenase

Short name=IMP dehydrogenase
Short name=IMPD
Short name=IMPDH
EC=1.1.1.205
Gene names
Name:impdh
Synonyms:guaB
ORF Names:DDB_G0283701
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity. HAMAP-Rule MF_03156

Catalytic activity

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH. HAMAP-Rule MF_03156

Cofactor

Potassium By similarity. HAMAP-Rule MF_03156

Enzyme regulation

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity. HAMAP-Rule MF_03156

Pathway

Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. HAMAP-Rule MF_03156

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03156

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_03156.

Induction

Down-regulated by Legionella pneumophila infection. Ref.3

Sequence similarities

Belongs to the IMPDH/GMPR family.

Contains 2 CBS domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Inosine-5'-monophosphate dehydrogenase HAMAP-Rule MF_03156
PRO_0000328152

Regions

Domain120 – 17960CBS 1
Domain183 – 23957CBS 2
Nucleotide binding277 – 2793NAD By similarity
Nucleotide binding327 – 3293NAD By similarity
Region367 – 3693IMP binding By similarity
Region390 – 3912IMP binding By similarity
Region414 – 4185IMP binding By similarity

Sites

Active site3341Thioimidate intermediate By similarity
Metal binding3291Potassium; via carbonyl oxygen By similarity
Metal binding3311Potassium; via carbonyl oxygen By similarity
Metal binding3341Potassium; via carbonyl oxygen By similarity
Metal binding5011Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal binding5021Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Binding site3321IMP By similarity
Binding site4421IMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54QQ0 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 054EFBD2EB940868

FASTA51556,311
        10         20         30         40         50         60 
MEKINKILQG TNSEYKSEWF VDGFDCFELF QQRHGYTYDD LIMLPGHINF SADDVSLKTK 

        70         80         90        100        110        120 
LTKNISLNAP LVSSPMDTVT EHLMAINMAL LGGIGIIHYN NTVEEQVVEV KKVKRFKNGF 

       130        140        150        160        170        180 
ITDPIVLSPT HKLSDVDMIK QKYGFSGIPI TDTGRIGGKL VGIVTSRDTD FIKDRSTTLS 

       190        200        210        220        230        240 
EVMTTDLITG QQNCTLEEAN SILKSCKKGK LPIVNDKGEL VALASRDDLV KNRDFPMATK 

       250        260        270        280        290        300 
DHENKKLLVG AALGTRETDK ERLAALSDAG VDVVILDSSQ GDSTYQREMI RFIKRNYPKI 

       310        320        330        340        350        360 
DVIGGNVVTT SQCESLIQAG VDGLRVGMGV GSICTTQEVM ACGRPQATAV FKCALYSSQY 

       370        380        390        400        410        420 
NVPIIADGGI RTIGHIIKGL SLGASSVMMG SMLAGTEEAP GDYFYKDGMR LKKYRGMGSL 

       430        440        450        460        470        480 
EAMVKGGDQR YFSETDKIKV AQGVSGSVVD KGSVKKFVPY LIQGIKHGLQ DLGCNSVTNL 

       490        500        510 
RESVYGGKVR FEVRTAAAQV EGSVHSLFSY EKHFI 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Veltman D.M., Insall R.H.
Submitted (JAN-2010) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 118-132; 143-155; 247-256 AND 431-455, IDENTIFICATION BY MASS SPECTROMETRY.
[3]"Dictyostelium transcriptional host cell response upon infection with Legionella."
Farbrother P., Wagner C., Na J., Tunggal B., Morio T., Urushihara H., Tanaka Y., Schleicher M., Steinert M., Eichinger L.
Cell. Microbiol. 8:438-456(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000056 Genomic DNA. Translation: EAL65617.1.
RefSeqXP_638973.1. XM_633881.1.

3D structure databases

ProteinModelPortalQ54QQ0.
SMRQ54QQ0. Positions 231-514.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230098.

Proteomic databases

PRIDEQ54QQ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230098; DDB0230098; DDB_G0283701.
GeneID8624219.
KEGGddi:DDB_G0283701.

Organism-specific databases

dictyBaseDDB_G0283701. guaB.

Phylogenomic databases

eggNOGCOG0517.
OMAFPEYEIT.
PhylomeDBQ54QQ0.

Enzyme and pathway databases

UniPathwayUPA00601; UER00295.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01964. IMPDH.
InterProIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFPIRSF000130. IMPDH. 1 hit.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsTIGR01302. IMP_dehydrog. 1 hit.
PROSITEPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54QQ0.

Entry information

Entry nameIMDH_DICDI
AccessionPrimary (citable) accession number: Q54QQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase