Q54QM6 (MPIP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase
EC=3.1.3.48

Alternative name(s):
Dual specificity phosphatase cdc25

Gene names

Name:	cdc25
ORF Names:	DDB_G0283617

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1053 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tyrosine protein phosphatase which may function as a dosage-dependent inducer in mitotic control By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Developmental stage	Present at all developmental stages (at protein level). Ref.1
Post-translational modification	Phosphorylated Probable. Ref.1
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.
Caution	The protein might be post-translationally truncated or the gene model could be wrong, because experimentally the protein migrates like a 56 kDa protein wereas it should migrate around 117 kDa.
Sequence caution	The sequence BAC66469.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from sequence or structural similarity Ref.1. Source: dictyBase cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	protein tyrosine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1053	1053	M-phase inducer phosphatase		PRO_0000327912
Regions
	Domain	901 – 1001	101	Rhodanese
	Compositional bias	45 – 61	17	Poly-Asn
	Compositional bias	106 – 111	6	Poly-Asn
	Compositional bias	131 – 139	9	Poly-Ser
	Compositional bias	168 – 207	40	Poly-Asn
	Compositional bias	313 – 336	24	Poly-Asn
	Compositional bias	382 – 411	30	Poly-Asn
	Compositional bias	564 – 597	34	Poly-Asn
	Compositional bias	685 – 688	4	Poly-Asn
	Compositional bias	716 – 724	9	Poly-Ser
	Compositional bias	725 – 731	7	Poly-Thr
	Compositional bias	744 – 764	21	Poly-Asn
	Compositional bias	813 – 822	10	Poly-Asn
	Compositional bias	851 – 857	7	Poly-Asn
	Compositional bias	1030 – 1035	6	Poly-Lys
Sites
	Active site	957	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54QM6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F528DD893056A22B	FASTA	1,053	117,119
	10 20 30 40 50 60 MGPTENQSFE IEFPNSSNTA AANSISTAIN NNTTTAATIT FTPANNNINN INNNNNNNNN 70 80 90 100 110 120 NKFNSNFDIE SNKENSINQS NINSNVNSFP LKIQTKTLNS TTTSSNNNNN NIFSIGFRPL 130 140 150 160 170 180 SPRGVSPRFH SNSSSSCSSE ISTPSSSTFL EDPNLIASNT ISSNNSINNN NNNNNNNNNN 190 200 210 220 230 240 NNNNNNDNSN NNINTNNINN NNNNTNNGEN PQKKQRNSQI LSKNFYSLSL HADSPKHSRS 250 260 270 280 290 300 TTDLSTFLGM SDPDVMTYRS KTFCISDFHQ FDIDTTTNDN KNKNNNTKST DESSTFDGKS 310 320 330 340 350 360 SNSVENNNMT IINNNNNNNN NNNNNNNNNN NNNNLNQDRP TIPTVAHSLS SNSIPHKFNS 370 380 390 400 410 420 SNSLPPQMLS SSAPSLFSLL ENSNNNNNNN NNNNNNNNNN NNNNNNNDNS NISINDESIV 430 440 450 460 470 480 LISTPVKEYS YNRSLCLSDE EGGEDDEDDT PPTPIQLVRP TPTPINPSLS LSLSSSLNAT 490 500 510 520 530 540 NSTTYLHLGR SATVLLNPPL LVSSMRQHHN SPSSSPSQSI PIVQTRERSQ SSLSFLSPYQ 550 560 570 580 590 600 QQYYPSSPEL SPMVPTPLVN RIFNNINNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN 610 620 630 640 650 660 SNNLNNQSTS EDRDDSPNTS LDQISGSPYK TPSKLSFHSP NSSAIFNSIS NSQQTGKRSR 670 680 690 700 710 720 TCLDFTKFSS HLLDEDSSCF STQTNNNNTS STGLISPSSS PKQQNVLIEN TTNTTSNSSS 730 740 750 760 770 780 SSSSTTTTTT TISVTNTLAA IQQNLNSNAN LNNNNNNNNN NNNNISSYFS RSNSISSQRP 790 800 810 820 830 840 PRPLSMSSSF ISRQSPDIHI SSNSLPKPVD TSNTNNNNEN ENDTNKLDKI NNVNSNIAKI 850 860 870 880 890 900 IEQQQQRNIS NSNNNNNLTQ DQDQQDDQFK AKLLKQLSGR QGYNGVSSES VYELLKNPTL 910 920 930 940 950 960 INAIITVVDC RYKYEYDGGH IKNAINIPPT GSRQMVLDRF FKFPTPKNQQ HVIIFHCEFS 970 980 990 1000 1010 1020 SKRAPDCYSL FRELDREHNE YPNIHYPEIY LLNGGYKKFF ESFQGDMCEG NYIRMDDKLY 1030 1040 1050 QANLKEEEEK KKKEKITIRN IKAFKNRSHS FHV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 in Dictyostelium discoideum." Mayanagi T., Maeda Y., Hirose S., Arakane T., Araki T., Amagai A. Dev. Genes Evol. 214:510-514(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems. AAFI02000056 Genomic DNA. Translation: EAL65575.1.
RefSeq	XP_638997.1. XM_633905.1.
3D structure databases
HSSP	HSSP built from PDB template 1YMK based on UniProtKB P30305.
ProteinModelPortal	Q54QM6.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191424.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191424; DDB0191424; DDB_G0283617.
GeneID	8624243.
KEGG	ddi:DDB_G0283617.
Organism-specific databases
dictyBase	DDB_G0283617. cdc25.
Phylogenomic databases
eggNOG	COG5105.
OMA	SSKRAPD.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPIP_DICDI

Accession

Primary (citable) accession number: Q54QM6
Secondary accession number(s): Q86M49

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)