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Q54QM6

- MPIP_DICDI

UniProt

Q54QM6 - MPIP_DICDI

Protein

M-phase inducer phosphatase

Gene

cdc25

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Tyrosine protein phosphatase which may function as a dosage-dependent inducer in mitotic control.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei957 – 9571Cysteine persulfide intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: dictyBase

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. peptidyl-tyrosine dephosphorylation Source: GOC
    3. protein dephosphorylation Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_213265. Cyclin A/B1 associated events during G2/M transition.
    REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
    REACT_217775. Cyclin B2 mediated events.
    REACT_226764. Polo-like kinase mediated events.
    REACT_226871. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_83228. p53-Independent DNA Damage Response.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase cdc25
    Gene namesi
    Name:cdc25
    ORF Names:DDB_G0283617
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283617. cdc25.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10531053M-phase inducer phosphatasePRO_0000327912Add
    BLAST

    Post-translational modificationi

    Phosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Present at all developmental stages (at protein level).1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0191424.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54QM6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini901 – 1001101RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi45 – 6117Poly-AsnAdd
    BLAST
    Compositional biasi106 – 1116Poly-Asn
    Compositional biasi131 – 1399Poly-Ser
    Compositional biasi168 – 20740Poly-AsnAdd
    BLAST
    Compositional biasi313 – 33624Poly-AsnAdd
    BLAST
    Compositional biasi382 – 41130Poly-AsnAdd
    BLAST
    Compositional biasi564 – 59734Poly-AsnAdd
    BLAST
    Compositional biasi685 – 6884Poly-Asn
    Compositional biasi716 – 7249Poly-Ser
    Compositional biasi725 – 7317Poly-Thr
    Compositional biasi744 – 76421Poly-AsnAdd
    BLAST
    Compositional biasi813 – 82210Poly-Asn
    Compositional biasi851 – 8577Poly-Asn
    Compositional biasi1030 – 10356Poly-Lys

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    OMAiSSKRAPD.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54QM6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPTENQSFE IEFPNSSNTA AANSISTAIN NNTTTAATIT FTPANNNINN     50
    INNNNNNNNN NKFNSNFDIE SNKENSINQS NINSNVNSFP LKIQTKTLNS 100
    TTTSSNNNNN NIFSIGFRPL SPRGVSPRFH SNSSSSCSSE ISTPSSSTFL 150
    EDPNLIASNT ISSNNSINNN NNNNNNNNNN NNNNNNDNSN NNINTNNINN 200
    NNNNTNNGEN PQKKQRNSQI LSKNFYSLSL HADSPKHSRS TTDLSTFLGM 250
    SDPDVMTYRS KTFCISDFHQ FDIDTTTNDN KNKNNNTKST DESSTFDGKS 300
    SNSVENNNMT IINNNNNNNN NNNNNNNNNN NNNNLNQDRP TIPTVAHSLS 350
    SNSIPHKFNS SNSLPPQMLS SSAPSLFSLL ENSNNNNNNN NNNNNNNNNN 400
    NNNNNNNDNS NISINDESIV LISTPVKEYS YNRSLCLSDE EGGEDDEDDT 450
    PPTPIQLVRP TPTPINPSLS LSLSSSLNAT NSTTYLHLGR SATVLLNPPL 500
    LVSSMRQHHN SPSSSPSQSI PIVQTRERSQ SSLSFLSPYQ QQYYPSSPEL 550
    SPMVPTPLVN RIFNNINNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN 600
    SNNLNNQSTS EDRDDSPNTS LDQISGSPYK TPSKLSFHSP NSSAIFNSIS 650
    NSQQTGKRSR TCLDFTKFSS HLLDEDSSCF STQTNNNNTS STGLISPSSS 700
    PKQQNVLIEN TTNTTSNSSS SSSSTTTTTT TISVTNTLAA IQQNLNSNAN 750
    LNNNNNNNNN NNNNISSYFS RSNSISSQRP PRPLSMSSSF ISRQSPDIHI 800
    SSNSLPKPVD TSNTNNNNEN ENDTNKLDKI NNVNSNIAKI IEQQQQRNIS 850
    NSNNNNNLTQ DQDQQDDQFK AKLLKQLSGR QGYNGVSSES VYELLKNPTL 900
    INAIITVVDC RYKYEYDGGH IKNAINIPPT GSRQMVLDRF FKFPTPKNQQ 950
    HVIIFHCEFS SKRAPDCYSL FRELDREHNE YPNIHYPEIY LLNGGYKKFF 1000
    ESFQGDMCEG NYIRMDDKLY QANLKEEEEK KKKEKITIRN IKAFKNRSHS 1050
    FHV 1053
    Length:1,053
    Mass (Da):117,119
    Last modified:May 24, 2005 - v1
    Checksum:iF528DD893056A22B
    GO

    Sequence cautioni

    The sequence BAC66469.2 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems.
    AAFI02000056 Genomic DNA. Translation: EAL65575.1.
    RefSeqiXP_638997.1. XM_633905.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191424; DDB0191424; DDB_G0283617.
    GeneIDi8624243.
    KEGGiddi:DDB_G0283617.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039883 Genomic DNA. Translation: BAC66469.2 . Sequence problems.
    AAFI02000056 Genomic DNA. Translation: EAL65575.1 .
    RefSeqi XP_638997.1. XM_633905.1.

    3D structure databases

    ProteinModelPortali Q54QM6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191424.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191424 ; DDB0191424 ; DDB_G0283617 .
    GeneIDi 8624243.
    KEGGi ddi:DDB_G0283617.

    Organism-specific databases

    dictyBasei DDB_G0283617. cdc25.

    Phylogenomic databases

    eggNOGi COG5105.
    OMAi SSKRAPD.

    Enzyme and pathway databases

    Reactomei REACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_213265. Cyclin A/B1 associated events during G2/M transition.
    REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
    REACT_217775. Cyclin B2 mediated events.
    REACT_226764. Polo-like kinase mediated events.
    REACT_226871. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_83228. p53-Independent DNA Damage Response.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 in Dictyostelium discoideum."
      Mayanagi T., Maeda Y., Hirose S., Arakane T., Araki T., Amagai A.
      Dev. Genes Evol. 214:510-514(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION.
      Strain: AX2.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMPIP_DICDI
    AccessioniPrimary (citable) accession number: Q54QM6
    Secondary accession number(s): Q86M49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The protein might be post-translationally truncated or the gene model could be wrong, because experimentally the protein migrates like a 56 kDa protein wereas it should migrate around 117 kDa.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3