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Protein

M-phase inducer phosphatase

Gene

cdc25

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase which may function as a dosage-dependent inducer in mitotic control.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei957 – 9571Cysteine persulfide intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: dictyBase

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
  3. peptidyl-tyrosine dephosphorylation Source: GOC
  4. protein dephosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase cdc25
Gene namesi
Name:cdc25
ORF Names:DDB_G0283617
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283617. cdc25.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10531053M-phase inducer phosphatasePRO_0000327912Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present at all developmental stages (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini901 – 1001101RhodanesePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi45 – 6117Poly-AsnAdd
BLAST
Compositional biasi106 – 1116Poly-Asn
Compositional biasi131 – 1399Poly-Ser
Compositional biasi168 – 20740Poly-AsnAdd
BLAST
Compositional biasi313 – 33624Poly-AsnAdd
BLAST
Compositional biasi382 – 41130Poly-AsnAdd
BLAST
Compositional biasi564 – 59734Poly-AsnAdd
BLAST
Compositional biasi685 – 6884Poly-Asn
Compositional biasi716 – 7249Poly-Ser
Compositional biasi725 – 7317Poly-Thr
Compositional biasi744 – 76421Poly-AsnAdd
BLAST
Compositional biasi813 – 82210Poly-Asn
Compositional biasi851 – 8577Poly-Asn
Compositional biasi1030 – 10356Poly-Lys

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
InParanoidiQ54QM6.
OMAiRSRTCLD.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54QM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPTENQSFE IEFPNSSNTA AANSISTAIN NNTTTAATIT FTPANNNINN
60 70 80 90 100
INNNNNNNNN NKFNSNFDIE SNKENSINQS NINSNVNSFP LKIQTKTLNS
110 120 130 140 150
TTTSSNNNNN NIFSIGFRPL SPRGVSPRFH SNSSSSCSSE ISTPSSSTFL
160 170 180 190 200
EDPNLIASNT ISSNNSINNN NNNNNNNNNN NNNNNNDNSN NNINTNNINN
210 220 230 240 250
NNNNTNNGEN PQKKQRNSQI LSKNFYSLSL HADSPKHSRS TTDLSTFLGM
260 270 280 290 300
SDPDVMTYRS KTFCISDFHQ FDIDTTTNDN KNKNNNTKST DESSTFDGKS
310 320 330 340 350
SNSVENNNMT IINNNNNNNN NNNNNNNNNN NNNNLNQDRP TIPTVAHSLS
360 370 380 390 400
SNSIPHKFNS SNSLPPQMLS SSAPSLFSLL ENSNNNNNNN NNNNNNNNNN
410 420 430 440 450
NNNNNNNDNS NISINDESIV LISTPVKEYS YNRSLCLSDE EGGEDDEDDT
460 470 480 490 500
PPTPIQLVRP TPTPINPSLS LSLSSSLNAT NSTTYLHLGR SATVLLNPPL
510 520 530 540 550
LVSSMRQHHN SPSSSPSQSI PIVQTRERSQ SSLSFLSPYQ QQYYPSSPEL
560 570 580 590 600
SPMVPTPLVN RIFNNINNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN
610 620 630 640 650
SNNLNNQSTS EDRDDSPNTS LDQISGSPYK TPSKLSFHSP NSSAIFNSIS
660 670 680 690 700
NSQQTGKRSR TCLDFTKFSS HLLDEDSSCF STQTNNNNTS STGLISPSSS
710 720 730 740 750
PKQQNVLIEN TTNTTSNSSS SSSSTTTTTT TISVTNTLAA IQQNLNSNAN
760 770 780 790 800
LNNNNNNNNN NNNNISSYFS RSNSISSQRP PRPLSMSSSF ISRQSPDIHI
810 820 830 840 850
SSNSLPKPVD TSNTNNNNEN ENDTNKLDKI NNVNSNIAKI IEQQQQRNIS
860 870 880 890 900
NSNNNNNLTQ DQDQQDDQFK AKLLKQLSGR QGYNGVSSES VYELLKNPTL
910 920 930 940 950
INAIITVVDC RYKYEYDGGH IKNAINIPPT GSRQMVLDRF FKFPTPKNQQ
960 970 980 990 1000
HVIIFHCEFS SKRAPDCYSL FRELDREHNE YPNIHYPEIY LLNGGYKKFF
1010 1020 1030 1040 1050
ESFQGDMCEG NYIRMDDKLY QANLKEEEEK KKKEKITIRN IKAFKNRSHS

FHV
Length:1,053
Mass (Da):117,119
Last modified:May 24, 2005 - v1
Checksum:iF528DD893056A22B
GO

Sequence cautioni

The sequence BAC66469.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems.
AAFI02000056 Genomic DNA. Translation: EAL65575.1.
RefSeqiXP_638997.1. XM_633905.1.

Genome annotation databases

EnsemblProtistsiDDB0191424; DDB0191424; DDB_G0283617.
GeneIDi8624243.
KEGGiddi:DDB_G0283617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems.
AAFI02000056 Genomic DNA. Translation: EAL65575.1.
RefSeqiXP_638997.1. XM_633905.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0191424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191424; DDB0191424; DDB_G0283617.
GeneIDi8624243.
KEGGiddi:DDB_G0283617.

Organism-specific databases

dictyBaseiDDB_G0283617. cdc25.

Phylogenomic databases

eggNOGiCOG5105.
InParanoidiQ54QM6.
OMAiRSRTCLD.

Miscellaneous databases

PROiQ54QM6.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 in Dictyostelium discoideum."
    Mayanagi T., Maeda Y., Hirose S., Arakane T., Araki T., Amagai A.
    Dev. Genes Evol. 214:510-514(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION.
    Strain: AX2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMPIP_DICDI
AccessioniPrimary (citable) accession number: Q54QM6
Secondary accession number(s): Q86M49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 29, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The protein might be post-translationally truncated or the gene model could be wrong, because experimentally the protein migrates like a 56 kDa protein wereas it should migrate around 117 kDa.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.