Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q54QM6 (MPIP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase

EC=3.1.3.48
Alternative name(s):
Dual specificity phosphatase cdc25
Gene names
Name:cdc25
ORF Names:DDB_G0283617
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1053 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine protein phosphatase which may function as a dosage-dependent inducer in mitotic control By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Developmental stage

Present at all developmental stages (at protein level). Ref.1

Post-translational modification

Phosphorylated Probable. Ref.1

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Caution

The protein might be post-translationally truncated or the gene model could be wrong, because experimentally the protein migrates like a 56 kDa protein wereas it should migrate around 117 kDa.

Sequence caution

The sequence BAC66469.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10531053M-phase inducer phosphatase
PRO_0000327912

Regions

Domain901 – 1001101Rhodanese
Compositional bias45 – 6117Poly-Asn
Compositional bias106 – 1116Poly-Asn
Compositional bias131 – 1399Poly-Ser
Compositional bias168 – 20740Poly-Asn
Compositional bias313 – 33624Poly-Asn
Compositional bias382 – 41130Poly-Asn
Compositional bias564 – 59734Poly-Asn
Compositional bias685 – 6884Poly-Asn
Compositional bias716 – 7249Poly-Ser
Compositional bias725 – 7317Poly-Thr
Compositional bias744 – 76421Poly-Asn
Compositional bias813 – 82210Poly-Asn
Compositional bias851 – 8577Poly-Asn
Compositional bias1030 – 10356Poly-Lys

Sites

Active site9571Cysteine persulfide intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54QM6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: F528DD893056A22B

FASTA1,053117,119
        10         20         30         40         50         60 
MGPTENQSFE IEFPNSSNTA AANSISTAIN NNTTTAATIT FTPANNNINN INNNNNNNNN 

        70         80         90        100        110        120 
NKFNSNFDIE SNKENSINQS NINSNVNSFP LKIQTKTLNS TTTSSNNNNN NIFSIGFRPL 

       130        140        150        160        170        180 
SPRGVSPRFH SNSSSSCSSE ISTPSSSTFL EDPNLIASNT ISSNNSINNN NNNNNNNNNN 

       190        200        210        220        230        240 
NNNNNNDNSN NNINTNNINN NNNNTNNGEN PQKKQRNSQI LSKNFYSLSL HADSPKHSRS 

       250        260        270        280        290        300 
TTDLSTFLGM SDPDVMTYRS KTFCISDFHQ FDIDTTTNDN KNKNNNTKST DESSTFDGKS 

       310        320        330        340        350        360 
SNSVENNNMT IINNNNNNNN NNNNNNNNNN NNNNLNQDRP TIPTVAHSLS SNSIPHKFNS 

       370        380        390        400        410        420 
SNSLPPQMLS SSAPSLFSLL ENSNNNNNNN NNNNNNNNNN NNNNNNNDNS NISINDESIV 

       430        440        450        460        470        480 
LISTPVKEYS YNRSLCLSDE EGGEDDEDDT PPTPIQLVRP TPTPINPSLS LSLSSSLNAT 

       490        500        510        520        530        540 
NSTTYLHLGR SATVLLNPPL LVSSMRQHHN SPSSSPSQSI PIVQTRERSQ SSLSFLSPYQ 

       550        560        570        580        590        600 
QQYYPSSPEL SPMVPTPLVN RIFNNINNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN 

       610        620        630        640        650        660 
SNNLNNQSTS EDRDDSPNTS LDQISGSPYK TPSKLSFHSP NSSAIFNSIS NSQQTGKRSR 

       670        680        690        700        710        720 
TCLDFTKFSS HLLDEDSSCF STQTNNNNTS STGLISPSSS PKQQNVLIEN TTNTTSNSSS 

       730        740        750        760        770        780 
SSSSTTTTTT TISVTNTLAA IQQNLNSNAN LNNNNNNNNN NNNNISSYFS RSNSISSQRP 

       790        800        810        820        830        840 
PRPLSMSSSF ISRQSPDIHI SSNSLPKPVD TSNTNNNNEN ENDTNKLDKI NNVNSNIAKI 

       850        860        870        880        890        900 
IEQQQQRNIS NSNNNNNLTQ DQDQQDDQFK AKLLKQLSGR QGYNGVSSES VYELLKNPTL 

       910        920        930        940        950        960 
INAIITVVDC RYKYEYDGGH IKNAINIPPT GSRQMVLDRF FKFPTPKNQQ HVIIFHCEFS 

       970        980        990       1000       1010       1020 
SKRAPDCYSL FRELDREHNE YPNIHYPEIY LLNGGYKKFF ESFQGDMCEG NYIRMDDKLY 

      1030       1040       1050 
QANLKEEEEK KKKEKITIRN IKAFKNRSHS FHV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 in Dictyostelium discoideum."
Mayanagi T., Maeda Y., Hirose S., Arakane T., Araki T., Amagai A.
Dev. Genes Evol. 214:510-514(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems.
AAFI02000056 Genomic DNA. Translation: EAL65575.1.
RefSeqXP_638997.1. XM_633905.1.

3D structure databases

ProteinModelPortalQ54QM6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191424.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191424; DDB0191424; DDB_G0283617.
GeneID8624243.
KEGGddi:DDB_G0283617.

Organism-specific databases

dictyBaseDDB_G0283617. cdc25.

Phylogenomic databases

eggNOGCOG5105.
OMASSKRAPD.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPIP_DICDI
AccessionPrimary (citable) accession number: Q54QM6
Secondary accession number(s): Q86M49
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase