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Q54QM6

- MPIP_DICDI

UniProt

Q54QM6 - MPIP_DICDI

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Protein
M-phase inducer phosphatase
Gene
cdc25, DDB_G0283617
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase which may function as a dosage-dependent inducer in mitotic control By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei957 – 9571Cysteine persulfide intermediate By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: dictyBase

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. peptidyl-tyrosine dephosphorylation Source: GOC
  3. protein dephosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_213265. Cyclin A/B1 associated events during G2/M transition.
REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
REACT_217775. Cyclin B2 mediated events.
REACT_226764. Polo-like kinase mediated events.
REACT_226871. Cyclin A:Cdk2-associated events at S phase entry.
REACT_83228. p53-Independent DNA Damage Response.
REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase cdc25
Gene namesi
Name:cdc25
ORF Names:DDB_G0283617
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283617. cdc25.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10531053M-phase inducer phosphatase
PRO_0000327912Add
BLAST

Post-translational modificationi

Phosphorylated Inferred.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present at all developmental stages (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191424.

Structurei

3D structure databases

ProteinModelPortaliQ54QM6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini901 – 1001101Rhodanese
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi45 – 6117Poly-Asn
Add
BLAST
Compositional biasi106 – 1116Poly-Asn
Compositional biasi131 – 1399Poly-Ser
Compositional biasi168 – 20740Poly-Asn
Add
BLAST
Compositional biasi313 – 33624Poly-Asn
Add
BLAST
Compositional biasi382 – 41130Poly-Asn
Add
BLAST
Compositional biasi564 – 59734Poly-Asn
Add
BLAST
Compositional biasi685 – 6884Poly-Asn
Compositional biasi716 – 7249Poly-Ser
Compositional biasi725 – 7317Poly-Thr
Compositional biasi744 – 76421Poly-Asn
Add
BLAST
Compositional biasi813 – 82210Poly-Asn
Compositional biasi851 – 8577Poly-Asn
Compositional biasi1030 – 10356Poly-Lys

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
OMAiSSKRAPD.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54QM6-1 [UniParc]FASTAAdd to Basket

« Hide

MGPTENQSFE IEFPNSSNTA AANSISTAIN NNTTTAATIT FTPANNNINN     50
INNNNNNNNN NKFNSNFDIE SNKENSINQS NINSNVNSFP LKIQTKTLNS 100
TTTSSNNNNN NIFSIGFRPL SPRGVSPRFH SNSSSSCSSE ISTPSSSTFL 150
EDPNLIASNT ISSNNSINNN NNNNNNNNNN NNNNNNDNSN NNINTNNINN 200
NNNNTNNGEN PQKKQRNSQI LSKNFYSLSL HADSPKHSRS TTDLSTFLGM 250
SDPDVMTYRS KTFCISDFHQ FDIDTTTNDN KNKNNNTKST DESSTFDGKS 300
SNSVENNNMT IINNNNNNNN NNNNNNNNNN NNNNLNQDRP TIPTVAHSLS 350
SNSIPHKFNS SNSLPPQMLS SSAPSLFSLL ENSNNNNNNN NNNNNNNNNN 400
NNNNNNNDNS NISINDESIV LISTPVKEYS YNRSLCLSDE EGGEDDEDDT 450
PPTPIQLVRP TPTPINPSLS LSLSSSLNAT NSTTYLHLGR SATVLLNPPL 500
LVSSMRQHHN SPSSSPSQSI PIVQTRERSQ SSLSFLSPYQ QQYYPSSPEL 550
SPMVPTPLVN RIFNNINNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN 600
SNNLNNQSTS EDRDDSPNTS LDQISGSPYK TPSKLSFHSP NSSAIFNSIS 650
NSQQTGKRSR TCLDFTKFSS HLLDEDSSCF STQTNNNNTS STGLISPSSS 700
PKQQNVLIEN TTNTTSNSSS SSSSTTTTTT TISVTNTLAA IQQNLNSNAN 750
LNNNNNNNNN NNNNISSYFS RSNSISSQRP PRPLSMSSSF ISRQSPDIHI 800
SSNSLPKPVD TSNTNNNNEN ENDTNKLDKI NNVNSNIAKI IEQQQQRNIS 850
NSNNNNNLTQ DQDQQDDQFK AKLLKQLSGR QGYNGVSSES VYELLKNPTL 900
INAIITVVDC RYKYEYDGGH IKNAINIPPT GSRQMVLDRF FKFPTPKNQQ 950
HVIIFHCEFS SKRAPDCYSL FRELDREHNE YPNIHYPEIY LLNGGYKKFF 1000
ESFQGDMCEG NYIRMDDKLY QANLKEEEEK KKKEKITIRN IKAFKNRSHS 1050
FHV 1053
Length:1,053
Mass (Da):117,119
Last modified:May 24, 2005 - v1
Checksum:iF528DD893056A22B
GO

Sequence cautioni

The sequence BAC66469.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039883 Genomic DNA. Translation: BAC66469.2. Sequence problems.
AAFI02000056 Genomic DNA. Translation: EAL65575.1.
RefSeqiXP_638997.1. XM_633905.1.

Genome annotation databases

EnsemblProtistsiDDB0191424; DDB0191424; DDB_G0283617.
GeneIDi8624243.
KEGGiddi:DDB_G0283617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039883 Genomic DNA. Translation: BAC66469.2 . Sequence problems.
AAFI02000056 Genomic DNA. Translation: EAL65575.1 .
RefSeqi XP_638997.1. XM_633905.1.

3D structure databases

ProteinModelPortali Q54QM6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191424.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191424 ; DDB0191424 ; DDB_G0283617 .
GeneIDi 8624243.
KEGGi ddi:DDB_G0283617.

Organism-specific databases

dictyBasei DDB_G0283617. cdc25.

Phylogenomic databases

eggNOGi COG5105.
OMAi SSKRAPD.

Enzyme and pathway databases

Reactomei REACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_213265. Cyclin A/B1 associated events during G2/M transition.
REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
REACT_217775. Cyclin B2 mediated events.
REACT_226764. Polo-like kinase mediated events.
REACT_226871. Cyclin A:Cdk2-associated events at S phase entry.
REACT_83228. p53-Independent DNA Damage Response.
REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 in Dictyostelium discoideum."
    Mayanagi T., Maeda Y., Hirose S., Arakane T., Araki T., Amagai A.
    Dev. Genes Evol. 214:510-514(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, PHOSPHORYLATION.
    Strain: AX2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMPIP_DICDI
AccessioniPrimary (citable) accession number: Q54QM6
Secondary accession number(s): Q86M49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The protein might be post-translationally truncated or the gene model could be wrong, because experimentally the protein migrates like a 56 kDa protein wereas it should migrate around 117 kDa.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3