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Q54QM4 (DNLI3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 3

EC=6.5.1.1
Alternative name(s):
DNA ligase III
Polydeoxyribonucleotide synthase [ATP] 3
Gene names
Name:lig3
ORF Names:DDB_G0283857
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1175 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The alpha isoform interactswith DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. The beta isoform doesnot interact with XRCC1 and may be specifically involved in the completion of homologous recombination events that occur during meiotic prophase.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11751175DNA ligase 3
PRO_0000351215

Regions

Domain883 – 97694BRCT 1
Domain1067 – 1174108BRCT 2
Compositional bias196 – 22429Asp-rich
Compositional bias225 – 2295Poly-Lys
Compositional bias622 – 63514Thr-rich
Compositional bias647 – 6559Poly-Asp
Compositional bias658 – 6614Poly-Gln
Compositional bias851 – 8599Poly-Ser
Compositional bias987 – 99610Poly-Ser
Compositional bias1016 – 10194Poly-Gln
Compositional bias1051 – 106010Poly-Asn
Compositional bias1117 – 115337Asn-rich

Sites

Active site2831N6-AMP-lysine intermediate By similarity
Metal binding3341Magnesium 1 Potential
Metal binding4321Magnesium 2 Potential
Binding site2811ATP By similarity
Binding site2881ATP By similarity
Binding site3031ATP By similarity
Binding site4371ATP By similarity
Binding site4481ATP By similarity
Binding site4521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54QM4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: D963D7D6EF49547E

FASTA1,175132,048
        10         20         30         40         50         60 
MSEDKSGSFY SMYKLCGQLE KESSHSGKTQ LIKSFCNVFK GDLYLLAKLM LCKEDKRVFR 

        70         80         90        100        110        120 
IKDKAMLKIC SHIWDCDLDD MIEDLDNGDF TETCKKFYIE YGKYPEKSTL TLKEVDQVLD 

       130        140        150        160        170        180 
SLTVSGKFED QVKIINKLLK RCTPFDFRLV CRIIDSDLKI NTGAKFFLDA FHPQAYDAFK 

       190        200        210        220        230        240 
KANNLKGVIE KIQQHDFDND NGDGDDGDGD DNDDDDGDGD SDSDKKKKKS SGGSGSDSGS 

       250        260        270        280        290        300 
KKKSKSFEVA IKLMTPIKPM LPKAVKTVEG VVKSSECFYA EIKYDGERIQ IHKDGNQFSC 

       310        320        330        340        350        360 
YSRNLKPLMP WKVDEVKPYI PKSTKAQQMI LDGEILLMDT KTSQPLPFGT LSAHKKNGFK 

       370        380        390        400        410        420 
DATVCVFLFD ILYLNGKSLI HLPLKERREI LEKNVMVVKN RIEFSEVTIV NGASEKSKLT 

       430        440        450        460        470        480 
ALLNRVFKEK LEGLVIKDAM SEYEPGCRHW IKIKKDYIHG MADSADLIAV GGYYGSGSMG 

       490        500        510        520        530        540 
GLVTVFLMVC YDKQNKIYKT VVKASGGLDD NMIAKLQPKV TSTMTKISKE VSKIPYWLDC 

       550        560        570        580        590        600 
PKQYAPDFIV KDIKQAMIFE IESAEFTKSD HHTTGYSMRF PRILKIRHDK DYKTATTLEE 

       610        620        630        640        650        660 
LVEIGKDIKI VPVGKGDNNS PTTTTTTTTT TTTTTSSKLI KKQQLSDDDD DNDKDSKQQQ 

       670        680        690        700        710        720 
QEPSKLKFVS DDLLDPFNDK SDDESNKIFI LNYVDNSGIW NEKGLSGAIG KKWPSIPKSF 

       730        740        750        760        770        780 
SQGDSNIIKS GEIRVEKVKD DSISTNKKVF ICNLSCIIPP KSKKESYTFS LKEFKSAIKE 

       790        800        810        820        830        840 
AKGAINQKKA SVHLAKPQFT SPSWSELDEV LTKELYNSGI KVFVHSISKS SPTTTSPTTT 

       850        860        870        880        890        900 
SPTTTSPKIT SPSSSSSPSN KLSPLKRGRD EKLEHEIIKD IEPSLPIFED VNAVIDSKTI 

       910        920        930        940        950        960 
DQVDVKRLIN SIKTMGGRVS DKWQQVGIGK TTHLICNGMS DLYLHVDRLG GTIVLPNWVD 

       970        980        990       1000       1010       1020 
QCFGSDKLLP LHEDYIYFNK KDHPDYSQSS SSSSMSIEEE KIVVTTTSDD PSEGNQQQQD 

      1030       1040       1050       1060       1070       1080 
KKVIKESKII QSKDHSSTTT IDTKITITST NNNNNGNDNN KTRKKQHLLS IFQECIIFLH 

      1090       1100       1110       1120       1130       1140 
DNVNDRETLK RYIIAYGGDI SDSVNDKTTH LVASLPNNFS NVKPKDLFKS IINNNSKNNN 

      1150       1160       1170 
NNNNNNNNGI IVNSLWLWDS INMSDLLDVK NYKLF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000057 Genomic DNA. Translation: EAL65543.1.

3D structure databases

ProteinModelPortalQ54QM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0232266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0232266; DDB0232266; DDB_G0283857.
KEGGddi:DDB_G0283857.

Organism-specific databases

dictyBaseDDB_G0283857. lig3.

Phylogenomic databases

eggNOGCOG1793.
KOK10776.
OMAINTGAKF.
PhylomeDBQ54QM4.
ProtClustDBCLSZ2736577.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54QM4.

Entry information

Entry nameDNLI3_DICDI
AccessionPrimary (citable) accession number: Q54QM4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase