Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q54QM4

- DNLI3_DICDI

UniProt

Q54QM4 - DNLI3_DICDI

Protein

DNA ligase 3

Gene

lig3

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The alpha isoform interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. The beta isoform does not interact with XRCC1 and may be specifically involved in the completion of homologous recombination events that occur during meiotic prophase.

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei281 – 2811ATPBy similarity
    Active sitei283 – 2831N6-AMP-lysine intermediatePROSITE-ProRule annotation
    Binding sitei288 – 2881ATPBy similarity
    Binding sitei303 – 3031ATPBy similarity
    Metal bindingi334 – 3341Magnesium 1Sequence Analysis
    Metal bindingi432 – 4321Magnesium 2Sequence Analysis
    Binding sitei437 – 4371ATPBy similarity
    Binding sitei448 – 4481ATPBy similarity
    Binding sitei452 – 4521ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: RefGenome
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation Source: RefGenome
    4. DNA ligation involved in DNA repair Source: InterPro
    5. DNA recombination Source: UniProtKB-KW
    6. lagging strand elongation Source: RefGenome
    7. nucleotide-excision repair Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 3 (EC:6.5.1.1)
    Alternative name(s):
    DNA ligase III
    Polydeoxyribonucleotide synthase [ATP] 3
    Gene namesi
    Name:lig3
    ORF Names:DDB_G0283857
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283857. lig3.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromosome Source: RefGenome
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11751175DNA ligase 3PRO_0000351215Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0232266.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54QM4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini883 – 97694BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1067 – 1174108BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi196 – 22429Asp-richAdd
    BLAST
    Compositional biasi225 – 2295Poly-Lys
    Compositional biasi622 – 63514Thr-richAdd
    BLAST
    Compositional biasi647 – 6559Poly-Asp
    Compositional biasi658 – 6614Poly-Gln
    Compositional biasi851 – 8599Poly-Ser
    Compositional biasi987 – 99610Poly-Ser
    Compositional biasi1016 – 10194Poly-Gln
    Compositional biasi1051 – 106010Poly-Asn
    Compositional biasi1117 – 115337Asn-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1793.
    KOiK10776.
    OMAiINTGAKF.
    PhylomeDBiQ54QM4.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF00533. BRCT. 2 hits.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 2 hits.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54QM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEDKSGSFY SMYKLCGQLE KESSHSGKTQ LIKSFCNVFK GDLYLLAKLM     50
    LCKEDKRVFR IKDKAMLKIC SHIWDCDLDD MIEDLDNGDF TETCKKFYIE 100
    YGKYPEKSTL TLKEVDQVLD SLTVSGKFED QVKIINKLLK RCTPFDFRLV 150
    CRIIDSDLKI NTGAKFFLDA FHPQAYDAFK KANNLKGVIE KIQQHDFDND 200
    NGDGDDGDGD DNDDDDGDGD SDSDKKKKKS SGGSGSDSGS KKKSKSFEVA 250
    IKLMTPIKPM LPKAVKTVEG VVKSSECFYA EIKYDGERIQ IHKDGNQFSC 300
    YSRNLKPLMP WKVDEVKPYI PKSTKAQQMI LDGEILLMDT KTSQPLPFGT 350
    LSAHKKNGFK DATVCVFLFD ILYLNGKSLI HLPLKERREI LEKNVMVVKN 400
    RIEFSEVTIV NGASEKSKLT ALLNRVFKEK LEGLVIKDAM SEYEPGCRHW 450
    IKIKKDYIHG MADSADLIAV GGYYGSGSMG GLVTVFLMVC YDKQNKIYKT 500
    VVKASGGLDD NMIAKLQPKV TSTMTKISKE VSKIPYWLDC PKQYAPDFIV 550
    KDIKQAMIFE IESAEFTKSD HHTTGYSMRF PRILKIRHDK DYKTATTLEE 600
    LVEIGKDIKI VPVGKGDNNS PTTTTTTTTT TTTTTSSKLI KKQQLSDDDD 650
    DNDKDSKQQQ QEPSKLKFVS DDLLDPFNDK SDDESNKIFI LNYVDNSGIW 700
    NEKGLSGAIG KKWPSIPKSF SQGDSNIIKS GEIRVEKVKD DSISTNKKVF 750
    ICNLSCIIPP KSKKESYTFS LKEFKSAIKE AKGAINQKKA SVHLAKPQFT 800
    SPSWSELDEV LTKELYNSGI KVFVHSISKS SPTTTSPTTT SPTTTSPKIT 850
    SPSSSSSPSN KLSPLKRGRD EKLEHEIIKD IEPSLPIFED VNAVIDSKTI 900
    DQVDVKRLIN SIKTMGGRVS DKWQQVGIGK TTHLICNGMS DLYLHVDRLG 950
    GTIVLPNWVD QCFGSDKLLP LHEDYIYFNK KDHPDYSQSS SSSSMSIEEE 1000
    KIVVTTTSDD PSEGNQQQQD KKVIKESKII QSKDHSSTTT IDTKITITST 1050
    NNNNNGNDNN KTRKKQHLLS IFQECIIFLH DNVNDRETLK RYIIAYGGDI 1100
    SDSVNDKTTH LVASLPNNFS NVKPKDLFKS IINNNSKNNN NNNNNNNNGI 1150
    IVNSLWLWDS INMSDLLDVK NYKLF 1175
    Length:1,175
    Mass (Da):132,048
    Last modified:May 24, 2005 - v1
    Checksum:iD963D7D6EF49547E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000057 Genomic DNA. Translation: EAL65543.1.
    RefSeqiXP_638847.1. XM_633755.1.

    Genome annotation databases

    EnsemblProtistsiDDB0232266; DDB0232266; DDB_G0283857.
    GeneIDi8624245.
    KEGGiddi:DDB_G0283857.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000057 Genomic DNA. Translation: EAL65543.1 .
    RefSeqi XP_638847.1. XM_633755.1.

    3D structure databases

    ProteinModelPortali Q54QM4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0232266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0232266 ; DDB0232266 ; DDB_G0283857 .
    GeneIDi 8624245.
    KEGGi ddi:DDB_G0283857.

    Organism-specific databases

    dictyBasei DDB_G0283857. lig3.

    Phylogenomic databases

    eggNOGi COG1793.
    KOi K10776.
    OMAi INTGAKF.
    PhylomeDBi Q54QM4.

    Miscellaneous databases

    PROi Q54QM4.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF00533. BRCT. 2 hits.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS00333. DNA_LIGASE_A2. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiDNLI3_DICDI
    AccessioniPrimary (citable) accession number: Q54QM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 23, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3