SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54QM4

- DNLI3_DICDI

UniProt

Q54QM4 - DNLI3_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA ligase 3
Gene
lig3, DDB_G0283857
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

The alpha isoform interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. The beta isoform does not interact with XRCC1 and may be specifically involved in the completion of homologous recombination events that occur during meiotic prophase.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei281 – 2811ATP By similarity
Active sitei283 – 2831N6-AMP-lysine intermediate By similarity
Binding sitei288 – 2881ATP By similarity
Binding sitei303 – 3031ATP By similarity
Metal bindingi334 – 3341Magnesium 1 Reviewed prediction
Metal bindingi432 – 4321Magnesium 2 Reviewed prediction
Binding sitei437 – 4371ATP By similarity
Binding sitei448 – 4481ATP By similarity
Binding sitei452 – 4521ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA ligation Source: RefGenome
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. cell cycle Source: UniProtKB-KW
  5. cell division Source: UniProtKB-KW
  6. lagging strand elongation Source: RefGenome
  7. nucleotide-excision repair Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 3 (EC:6.5.1.1)
Alternative name(s):
DNA ligase III
Polydeoxyribonucleotide synthase [ATP] 3
Gene namesi
Name:lig3
ORF Names:DDB_G0283857
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283857. lig3.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11751175DNA ligase 3
PRO_0000351215Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0232266.

Structurei

3D structure databases

ProteinModelPortaliQ54QM4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini883 – 97694BRCT 1
Add
BLAST
Domaini1067 – 1174108BRCT 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi196 – 22429Asp-rich
Add
BLAST
Compositional biasi225 – 2295Poly-Lys
Compositional biasi622 – 63514Thr-rich
Add
BLAST
Compositional biasi647 – 6559Poly-Asp
Compositional biasi658 – 6614Poly-Gln
Compositional biasi851 – 8599Poly-Ser
Compositional biasi987 – 99610Poly-Ser
Compositional biasi1016 – 10194Poly-Gln
Compositional biasi1051 – 106010Poly-Asn
Compositional biasi1117 – 115337Asn-rich
Add
BLAST

Sequence similaritiesi

Contains 2 BRCT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
KOiK10776.
OMAiINTGAKF.
PhylomeDBiQ54QM4.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54QM4-1 [UniParc]FASTAAdd to Basket

« Hide

MSEDKSGSFY SMYKLCGQLE KESSHSGKTQ LIKSFCNVFK GDLYLLAKLM     50
LCKEDKRVFR IKDKAMLKIC SHIWDCDLDD MIEDLDNGDF TETCKKFYIE 100
YGKYPEKSTL TLKEVDQVLD SLTVSGKFED QVKIINKLLK RCTPFDFRLV 150
CRIIDSDLKI NTGAKFFLDA FHPQAYDAFK KANNLKGVIE KIQQHDFDND 200
NGDGDDGDGD DNDDDDGDGD SDSDKKKKKS SGGSGSDSGS KKKSKSFEVA 250
IKLMTPIKPM LPKAVKTVEG VVKSSECFYA EIKYDGERIQ IHKDGNQFSC 300
YSRNLKPLMP WKVDEVKPYI PKSTKAQQMI LDGEILLMDT KTSQPLPFGT 350
LSAHKKNGFK DATVCVFLFD ILYLNGKSLI HLPLKERREI LEKNVMVVKN 400
RIEFSEVTIV NGASEKSKLT ALLNRVFKEK LEGLVIKDAM SEYEPGCRHW 450
IKIKKDYIHG MADSADLIAV GGYYGSGSMG GLVTVFLMVC YDKQNKIYKT 500
VVKASGGLDD NMIAKLQPKV TSTMTKISKE VSKIPYWLDC PKQYAPDFIV 550
KDIKQAMIFE IESAEFTKSD HHTTGYSMRF PRILKIRHDK DYKTATTLEE 600
LVEIGKDIKI VPVGKGDNNS PTTTTTTTTT TTTTTSSKLI KKQQLSDDDD 650
DNDKDSKQQQ QEPSKLKFVS DDLLDPFNDK SDDESNKIFI LNYVDNSGIW 700
NEKGLSGAIG KKWPSIPKSF SQGDSNIIKS GEIRVEKVKD DSISTNKKVF 750
ICNLSCIIPP KSKKESYTFS LKEFKSAIKE AKGAINQKKA SVHLAKPQFT 800
SPSWSELDEV LTKELYNSGI KVFVHSISKS SPTTTSPTTT SPTTTSPKIT 850
SPSSSSSPSN KLSPLKRGRD EKLEHEIIKD IEPSLPIFED VNAVIDSKTI 900
DQVDVKRLIN SIKTMGGRVS DKWQQVGIGK TTHLICNGMS DLYLHVDRLG 950
GTIVLPNWVD QCFGSDKLLP LHEDYIYFNK KDHPDYSQSS SSSSMSIEEE 1000
KIVVTTTSDD PSEGNQQQQD KKVIKESKII QSKDHSSTTT IDTKITITST 1050
NNNNNGNDNN KTRKKQHLLS IFQECIIFLH DNVNDRETLK RYIIAYGGDI 1100
SDSVNDKTTH LVASLPNNFS NVKPKDLFKS IINNNSKNNN NNNNNNNNGI 1150
IVNSLWLWDS INMSDLLDVK NYKLF 1175
Length:1,175
Mass (Da):132,048
Last modified:May 24, 2005 - v1
Checksum:iD963D7D6EF49547E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000057 Genomic DNA. Translation: EAL65543.1.
RefSeqiXP_638847.1. XM_633755.1.

Genome annotation databases

EnsemblProtistsiDDB0232266; DDB0232266; DDB_G0283857.
GeneIDi8624245.
KEGGiddi:DDB_G0283857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000057 Genomic DNA. Translation: EAL65543.1 .
RefSeqi XP_638847.1. XM_633755.1.

3D structure databases

ProteinModelPortali Q54QM4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0232266.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0232266 ; DDB0232266 ; DDB_G0283857 .
GeneIDi 8624245.
KEGGi ddi:DDB_G0283857.

Organism-specific databases

dictyBasei DDB_G0283857. lig3.

Phylogenomic databases

eggNOGi COG1793.
KOi K10776.
OMAi INTGAKF.
PhylomeDBi Q54QM4.

Miscellaneous databases

PROi Q54QM4.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiDNLI3_DICDI
AccessioniPrimary (citable) accession number: Q54QM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi