Reviewed, UniProtKB/Swiss-Prot Q54QI4 (HGD_DICDI)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisic acid oxidase
    Homogentisate oxygenase
    Homogentisicase

Gene names

Name:	hgd
Synonyms:	hgo
ORF Names:	DDB_G0283765

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Homogentisate 1,2-dioxygenase		PRO_0000312407
Sites
	Metal binding	333	1	Iron By similarity
	Metal binding	339	1	Iron By similarity
	Metal binding	369	1	Iron By similarity
Experimental info
	Sequence conflict	2 – 4	3	ALE → TRP in AAG17115. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q54QI4-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F704C3DCA58CAC53	FASTA	432	48,234
	10 20 30 40 50 60 MALEQIDYEY QSGFGNSFES EAIKGTLPKG RNAPQNCPLD LYAEQLSGNA FTAPRHTQQR 70 80 90 100 110 120 SWLYRIRPSV CHTPLKPIDS GLVCDLNNLH VDPNQLRWKP FPITEDKPHD FVEGLITIAG 130 140 150 160 170 180 AGHASVRHGL AIHIYTATKS MENKSFYNSD GDFLIVPQQG TLDIQTEFGF MKVKSGEICV 190 200 210 220 230 240 IQRGITFSVN VEGPTRGYIC EVFGSHFKLP DLGPIGANGL ANPRDFLSPV AAYEKKEGIE 250 260 270 280 290 300 HTKINKFLGK LFSATQTYSP FNVVAWHGNY CPYKYDLSLF CVVNSVSFDH LDPSIFTVLT 310 320 330 340 350 360 APTNEVGVAA ADFVIFPPRW LVQENTFRPP YFHRNCMSEF MGLIRGVYEA KKEGFLPGGG 370 380 390 400 410 420 SLHSCMTPHG PDSDTFYAAI KAELKPTKIP DVALAFMFES SLILGISDYA KKNFIDDDYW 430 KCWQGLKDNS KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Identification of HGO homologs in different organisms." Schmidt S.R. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-432.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000057 Genomic DNA. Translation: EAL65497.1. AF189237 mRNA. Translation: AAG17115.1.
RefSeq	XP_638887.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3390419.
KEGG	ddi:DDB_0191461.
Organism-specific databases
dictyBase	DDB_G0283765. hgd.
Phylogenomic databases
OMA	Q54QI4. HRNCMSE.
Enzyme and pathway databases
BRENDA	1.13.11.5. 424.
Family and domain databases
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_DICDI

Accession

Primary (citable) accession number: Q54QI4
Secondary accession number(s): Q9GU65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)