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Q54QG0

- PGFB_DICDI

UniProt

Q54QG0 - PGFB_DICDI

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Protein

Bifunctional glycosyltransferase pgtA

Gene

pgtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional protein composed of 2 glycosyltransferase domains involved in glycosylating skp1. The N-terminal part catalyzes the transfer of a galactose residue to GlcNAc-skp1 in a beta 1-3 linkage. The C-terminal part catalyzes the transfer of a fucose residue to Gal-GlgNAc-skp1 in an alpha 1-2 linkage.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + alpha-N-acetylglucosamine-R = UDP + beta-D-galactosyl-1,3-alpha-N-acetylglucosamine-R.
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei53 – 531Important for Gal-transferase activity
Sitei226 – 2261Important for Gal-transferase activity
Sitei509 – 5091Important for Gal- and Fuc-transferase activities
Sitei654 – 6541Important for Fuc-transferase activity

GO - Molecular functioni

  1. alpha-(1,2)-fucosyltransferase activity Source: dictyBase
  2. fucosyltransferase activity Source: dictyBase
  3. galactoside 2-alpha-L-fucosyltransferase activity Source: UniProtKB-EC
  4. glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity Source: dictyBase

GO - Biological processi

  1. fucosylation Source: GOC
  2. protein glycosylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.
GT74. Glycosyltransferase Family 74.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glycosyltransferase pgtA
Alternative name(s):
FT85
Including the following 2 domains:
N-acetylglucosamine 3-beta-galactosyltransferase (EC:2.4.1.-)
Alpha-1,2-fucosyltransferase (EC:2.4.1.69)
Gene namesi
Name:pgtA
Synonyms:fucB
ORF Names:DDB_G0283761
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283761. pgtA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi53 – 531D → N: Loss of Gal-transferase activity. 1 Publication
Mutagenesisi226 – 2261D → N: Loss of Gal-transferase activity. 1 Publication
Mutagenesisi497 – 4971D → N: Slight decrease in Gal-transferase activity. 1 Publication
Mutagenesisi509 – 5091D → N: 39-60% decrease in both Gal- and Fuc-transferase activities. 1 Publication
Mutagenesisi654 – 6541D → N: Loss of Fuc-transferase activity and 80% decrease in Gal-transferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 769769Bifunctional glycosyltransferase pgtAPRO_0000328252Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191458.

Structurei

3D structure databases

ProteinModelPortaliQ54QG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 210186N-acetylgalactosamine 3-beta-galactosyltransferaseAdd
BLAST
Regioni442 – 769328Alpha-1,2-fucosyltransferaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi151 – 16212Poly-AsnAdd
BLAST
Compositional biasi409 – 44133Poly-AsnAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiCOG0463.
InParanoidiQ54QG0.
OMAiNITYCHT.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q54QG0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNDSPIISVV LPFLIKDNDD KSLNYQGINN LIISIDSIIE QTFKEWELIL
60 70 80 90 100
VDDGSNNEIL EQLLSKRYST DNRIKFIINK ENKGIVKSLN DAILNHCSPT
110 120 130 140 150
SKYIARMDSD DISHPTRLQS QLKYLQSNET IDILGCPIKM FNNNKLIEIL
160 170 180 190 200
NNNNNNNNIN NNVKELINII NNEESFKFIQ HPDKDILMWS MFFNCCIVHP
210 220 230 240 250
SVIFKRSIFT IEHCYEENNQ FPFIEDYLFW LKSLIMKGLN ISNIQSSTPL
260 270 280 290 300
LYLRKHNNSI SFKNIEKQKD STANASCYYL NILFKRFNID SEIIQNSSLS
310 320 330 340 350
MKEIIQFFQL SPSSLSKINN ISIELFEFAF KYLELIEKSC TKQQPNYSNS
360 370 380 390 400
IKDAANEKMG ELVSLCLSNY PNNQKSSLLW EKWLSRNPTS QLLSLLSNLN
410 420 430 440 450
VKSSTTIINN NINNNNNNNN NNNNNNNNNN NNNNNNNNNN NSILNFISGI
460 470 480 490 500
NSNKINTPKS NNNKFKENGI RIICFSKDRA FQLKEYLRTF FKYLKNDDNG
510 520 530 540 550
NDKFEIIVDV LFTYSNEKFK NSYQLVIESF PQVNFIKEEN FTDQLINLVQ
560 570 580 590 600
KTNKLEYVMF SVDDILYYNE FNLKEYCLSL NSEPLALGFY MKLNKNITYC
610 620 630 640 650
HTCNQDITIP LNSNTISRTE NNFKYLKWNR NDNDCKKDWN YPWDLCSTIY
660 670 680 690 700
RCNDIDSIIN GIVKYYGIRN GINHPNRFEF NGNRPIIQKQ IYQNKPYCLC
710 720 730 740 750
LSDHYSPMSV VTINRVQDVY DNPIYDQTLS LDDLDQLLYS NKSLNDEKYK
760
ENSLSLNFKS VHIGELFIS
Length:769
Mass (Da):89,838
Last modified:May 24, 2005 - v1
Checksum:i8BB93A4F528C0D84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti413 – 4131Missing in AAF82378. (PubMed:11423539)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF279134 Genomic DNA. Translation: AAF82378.1.
AAFI02000057 Genomic DNA. Translation: EAL65495.1.
RefSeqiXP_638911.1. XM_633819.1.

Genome annotation databases

EnsemblProtistsiDDB0191458; DDB0191458; DDB_G0283761.
GeneIDi8624308.
KEGGiddi:DDB_G0283761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF279134 Genomic DNA. Translation: AAF82378.1 .
AAFI02000057 Genomic DNA. Translation: EAL65495.1 .
RefSeqi XP_638911.1. XM_633819.1.

3D structure databases

ProteinModelPortali Q54QG0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191458.

Protein family/group databases

CAZyi GT2. Glycosyltransferase Family 2.
GT74. Glycosyltransferase Family 74.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191458 ; DDB0191458 ; DDB_G0283761 .
GeneIDi 8624308.
KEGGi ddi:DDB_G0283761.

Organism-specific databases

dictyBasei DDB_G0283761. pgtA.

Phylogenomic databases

eggNOGi COG0463.
InParanoidi Q54QG0.
OMAi NITYCHT.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A non-Golgi alpha 1,2-fucosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
    van Der Wel H., Morris H.R., Panico M., Paxton T., North S.J., Dell A., Thomson J.M., West C.M.
    J. Biol. Chem. 276:33952-33963(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PROTEIN SEQUENCE OF 146-184; 238-263; 288-302 AND 343-352, IDENTIFICATION BY MASS SPECTROMETRY.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "A bifunctional diglycosyltransferase forms the Fucalpha1,2Galbeta1,3-disaccharide on Skp1 in the cytoplasm of dictyostelium."
    Van Der Wel H., Fisher S.Z., West C.M.
    J. Biol. Chem. 277:46527-46534(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, MUTAGENESIS OF ASP-53; ASP-226; ASP-497; ASP-509 AND ASP-654.

Entry informationi

Entry nameiPGFB_DICDI
AccessioniPrimary (citable) accession number: Q54QG0
Secondary accession number(s): Q9NAX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3