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Q54QG0

- PGFB_DICDI

UniProt

Q54QG0 - PGFB_DICDI

Protein

Bifunctional glycosyltransferase pgtA

Gene

pgtA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Bifunctional protein composed of 2 glycosyltransferase domains involved in glycosylating skp1. The N-terminal part catalyzes the transfer of a galactose residue to GlcNAc-skp1 in a beta 1-3 linkage. The C-terminal part catalyzes the transfer of a fucose residue to Gal-GlgNAc-skp1 in an alpha 1-2 linkage.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + alpha-N-acetylglucosamine-R = UDP + beta-D-galactosyl-1,3-alpha-N-acetylglucosamine-R.
    GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei53 – 531Important for Gal-transferase activity
    Sitei226 – 2261Important for Gal-transferase activity
    Sitei509 – 5091Important for Gal- and Fuc-transferase activities
    Sitei654 – 6541Important for Fuc-transferase activity

    GO - Molecular functioni

    1. alpha-(1,2)-fucosyltransferase activity Source: dictyBase
    2. fucosyltransferase activity Source: dictyBase
    3. galactoside 2-alpha-L-fucosyltransferase activity Source: UniProtKB-EC
    4. glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity Source: dictyBase

    GO - Biological processi

    1. fucosylation Source: GOC
    2. protein glycosylation Source: dictyBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.
    GT74. Glycosyltransferase Family 74.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional glycosyltransferase pgtA
    Alternative name(s):
    FT85
    Including the following 2 domains:
    N-acetylglucosamine 3-beta-galactosyltransferase (EC:2.4.1.-)
    Alpha-1,2-fucosyltransferase (EC:2.4.1.69)
    Gene namesi
    Name:pgtA
    Synonyms:fucB
    ORF Names:DDB_G0283761
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0283761. pgtA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531D → N: Loss of Gal-transferase activity. 1 Publication
    Mutagenesisi226 – 2261D → N: Loss of Gal-transferase activity. 1 Publication
    Mutagenesisi497 – 4971D → N: Slight decrease in Gal-transferase activity. 1 Publication
    Mutagenesisi509 – 5091D → N: 39-60% decrease in both Gal- and Fuc-transferase activities. 1 Publication
    Mutagenesisi654 – 6541D → N: Loss of Fuc-transferase activity and 80% decrease in Gal-transferase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 769769Bifunctional glycosyltransferase pgtAPRO_0000328252Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0191458.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54QG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 210186N-acetylgalactosamine 3-beta-galactosyltransferaseAdd
    BLAST
    Regioni442 – 769328Alpha-1,2-fucosyltransferaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi151 – 16212Poly-AsnAdd
    BLAST
    Compositional biasi409 – 44133Poly-AsnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0463.
    OMAiNITYCHT.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q54QG0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDSPIISVV LPFLIKDNDD KSLNYQGINN LIISIDSIIE QTFKEWELIL    50
    VDDGSNNEIL EQLLSKRYST DNRIKFIINK ENKGIVKSLN DAILNHCSPT 100
    SKYIARMDSD DISHPTRLQS QLKYLQSNET IDILGCPIKM FNNNKLIEIL 150
    NNNNNNNNIN NNVKELINII NNEESFKFIQ HPDKDILMWS MFFNCCIVHP 200
    SVIFKRSIFT IEHCYEENNQ FPFIEDYLFW LKSLIMKGLN ISNIQSSTPL 250
    LYLRKHNNSI SFKNIEKQKD STANASCYYL NILFKRFNID SEIIQNSSLS 300
    MKEIIQFFQL SPSSLSKINN ISIELFEFAF KYLELIEKSC TKQQPNYSNS 350
    IKDAANEKMG ELVSLCLSNY PNNQKSSLLW EKWLSRNPTS QLLSLLSNLN 400
    VKSSTTIINN NINNNNNNNN NNNNNNNNNN NNNNNNNNNN NSILNFISGI 450
    NSNKINTPKS NNNKFKENGI RIICFSKDRA FQLKEYLRTF FKYLKNDDNG 500
    NDKFEIIVDV LFTYSNEKFK NSYQLVIESF PQVNFIKEEN FTDQLINLVQ 550
    KTNKLEYVMF SVDDILYYNE FNLKEYCLSL NSEPLALGFY MKLNKNITYC 600
    HTCNQDITIP LNSNTISRTE NNFKYLKWNR NDNDCKKDWN YPWDLCSTIY 650
    RCNDIDSIIN GIVKYYGIRN GINHPNRFEF NGNRPIIQKQ IYQNKPYCLC 700
    LSDHYSPMSV VTINRVQDVY DNPIYDQTLS LDDLDQLLYS NKSLNDEKYK 750
    ENSLSLNFKS VHIGELFIS 769
    Length:769
    Mass (Da):89,838
    Last modified:May 24, 2005 - v1
    Checksum:i8BB93A4F528C0D84
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti413 – 4131Missing in AAF82378. (PubMed:11423539)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF279134 Genomic DNA. Translation: AAF82378.1.
    AAFI02000057 Genomic DNA. Translation: EAL65495.1.
    RefSeqiXP_638911.1. XM_633819.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191458; DDB0191458; DDB_G0283761.
    GeneIDi8624308.
    KEGGiddi:DDB_G0283761.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF279134 Genomic DNA. Translation: AAF82378.1 .
    AAFI02000057 Genomic DNA. Translation: EAL65495.1 .
    RefSeqi XP_638911.1. XM_633819.1.

    3D structure databases

    ProteinModelPortali Q54QG0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191458.

    Protein family/group databases

    CAZyi GT2. Glycosyltransferase Family 2.
    GT74. Glycosyltransferase Family 74.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191458 ; DDB0191458 ; DDB_G0283761 .
    GeneIDi 8624308.
    KEGGi ddi:DDB_G0283761.

    Organism-specific databases

    dictyBasei DDB_G0283761. pgtA.

    Phylogenomic databases

    eggNOGi COG0463.
    OMAi NITYCHT.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "A non-Golgi alpha 1,2-fucosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium."
      van Der Wel H., Morris H.R., Panico M., Paxton T., North S.J., Dell A., Thomson J.M., West C.M.
      J. Biol. Chem. 276:33952-33963(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PROTEIN SEQUENCE OF 146-184; 238-263; 288-302 AND 343-352, IDENTIFICATION BY MASS SPECTROMETRY.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "A bifunctional diglycosyltransferase forms the Fucalpha1,2Galbeta1,3-disaccharide on Skp1 in the cytoplasm of dictyostelium."
      Van Der Wel H., Fisher S.Z., West C.M.
      J. Biol. Chem. 277:46527-46534(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, MUTAGENESIS OF ASP-53; ASP-226; ASP-497; ASP-509 AND ASP-654.

    Entry informationi

    Entry nameiPGFB_DICDI
    AccessioniPrimary (citable) accession number: Q54QG0
    Secondary accession number(s): Q9NAX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3