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Protein

NAD-dependent deacetylase sir2A

Gene

sir2A

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NAD-dependent deacetylase, which plays an important role in the regulation of transcriptional repression.By similarity

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei361Proton acceptorPROSITE-ProRule annotation1
Metal bindingi369ZincPROSITE-ProRule annotation1
Metal bindingi372ZincPROSITE-ProRule annotation1
Metal bindingi393ZincPROSITE-ProRule annotation1
Metal bindingi399ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri32 – 93UBP-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent deacetylase sir2A (EC:3.5.1.-)
Alternative name(s):
Silent information regulator sir2A
Gene namesi
Name:sir2A
ORF Names:DDB_G0283917
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283917. sir2A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003931251 – 512NAD-dependent deacetylase sir2AAdd BLAST512

Proteomic databases

PaxDbiQ54QE6.

Expressioni

Developmental stagei

Expressed at high levels in growing cells, but at decreased levels in developing cells. Expressed in both prestalk and prespore cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0216430.

Structurei

3D structure databases

ProteinModelPortaliQ54QE6.
SMRiQ54QE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 505Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST267

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi123 – 127Poly-Glu5
Compositional biasi154 – 157Poly-Thr4
Compositional biasi158 – 161Poly-Ser4
Compositional biasi162 – 172Poly-ThrAdd BLAST11
Compositional biasi177 – 185Poly-Glu9

Sequence similaritiesi

Belongs to the sirtuin family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri32 – 93UBP-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1343. Eukaryota.
KOG2682. Eukaryota.
COG0846. LUCA.
InParanoidiQ54QE6.
KOiK11412.
OMAiCHFISAT.
PhylomeDBiQ54QE6.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.40.50.1220. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026590. Ssirtuin_cat_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF02146. SIR2. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiView protein in PROSITE
PS50305. SIRTUIN. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54QE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAVNPIECI HLKDEYDTCI NKSIFDKDLK ITKCHACNDE SENWICMTCG
60 70 80 90 100
VVSCSRHVNG HAGEHFENTK HPISASFSDH SFWCYTCDTY VHNTPLFDIC
110 120 130 140 150
EILENIKSSN KKDKIVPKKD QKEEEEEDQV VPSASITTSS TTTSISKQTT
160 170 180 190 200
VNNTTTTSSS STTTTTTTTS TTINNNEEEE ESESETDESS SEGEESLSLI
210 220 230 240 250
QRMREMIFGV GRGPKIVAPS EQEESEEDES CVLKKPTIEE IAKYINSAKC
260 270 280 290 300
KNIIVMTGAG ISVAAGIPDF RSPKTGLYEK LDKYDLPYRE AIFDIEYFKK
310 320 330 340 350
NPKPFYVLSK ELFPGSFNPT TVHYFIKLLS DKGLLLRNFT QNIDTLERIA
360 370 380 390 400
GIPANKLVEA HGSFATSHCV SCKKEYSTEY VKERIFKDEL PECTETSGCK
410 420 430 440 450
GIVKPDIVFF GESLPSRFND CAREDFTKCD LLLVIGTSLK VHPFASLINF
460 470 480 490 500
AKGCPRVLIN FEEVGTNPYG GFKFNQPSNK LDVKCIGDCQ TLVLDLIKLL
510
GWENEFNQIV KN
Length:512
Mass (Da):57,413
Last modified:May 24, 2005 - v1
Checksum:iE117B76700157565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000058 Genomic DNA. Translation: EAL65446.1.
RefSeqiXP_638798.1. XM_633706.1.

Genome annotation databases

EnsemblProtistsiEAL65446; EAL65446; DDB_G0283917.
GeneIDi8624322.
KEGGiddi:DDB_G0283917.

Similar proteinsi

Entry informationi

Entry nameiSIR2A_DICDI
AccessioniPrimary (citable) accession number: Q54QE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: May 24, 2005
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families