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Q54Q71 (RIR1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase large subunit

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene names
Name:rnrA
ORF Names:DDB_G0284071
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulation

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterodimer of a large and a small subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Contains 1 ATP-cone domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Ribonucleoside-diphosphate reductase large subunit
PRO_0000328498

Regions

Domain16 – 11095ATP-cone
Region26 – 327Allosteric activator binding By similarity
Region235 – 2362Substrate binding By similarity
Region303 – 3064Allosteric effector binding, determines substrate specificity By similarity
Region446 – 4505Substrate binding By similarity
Region631 – 6355Substrate binding By similarity
Compositional bias832 – 84413Poly-Gln

Sites

Active site4461Proton acceptor By similarity
Active site4481Cysteine radical intermediate By similarity
Active site4501Proton acceptor By similarity
Binding site201Allosteric activator By similarity
Binding site711Allosteric activator By similarity
Binding site1061Allosteric activator By similarity
Binding site2201Substrate By similarity
Binding site2651Substrate; via amide nitrogen By similarity
Site2361Important for hydrogen atom transfer By similarity
Site2441Allosteric effector binding, determines substrate specificity By similarity
Site2741Allosteric effector binding, determines substrate specificity By similarity
Site4631Important for hydrogen atom transfer By similarity
Site7651Important for electron transfer By similarity
Site7661Important for electron transfer By similarity
Site8651Interacts with thioredoxin/glutaredoxin By similarity
Site8681Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond236 ↔ 463Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54Q71 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 41A13B6274074897

FASTA87098,019
        10         20         30         40         50         60 
MISNSINVTP NKESKMYVVK RDGTKENVSF DKITSRISFL CEMEPKLNSD IVDPIEVAQK 

        70         80         90        100        110        120 
VVSGVFPGVK TTELDNLAAE TAAYMSTRHP DYGILAARIS VSNLHKQTSK SFVETVKKQY 

       130        140        150        160        170        180 
EFINPKSNLL APLVSKELYE IVMKHGERLE SVIDYYRDFD YDFFGFKTLE RSYLHRINGK 

       190        200        210        220        230        240 
IVERPQHMLM RVSIGIHGED LESAINTYKR LSEKLFTHAT PTLFNAGTPS PQMSSCFLVQ 

       250        260        270        280        290        300 
MKEDSIDGIY DTLKQCALIS KSAGGIGIAV HKIRAAQSYI RGTNGTSNGL VPMLRVFNDT 

       310        320        330        340        350        360 
ARYVDQGGGK RKGAFAVYLE PWHADVFEFI DLKKNTGKEE MRARDLFYAL WVSDIFMERV 

       370        380        390        400        410        420 
EQNGDWALFC PNEAPGLAET HSEEHRALYL KYEATPDLPR RVIKAQELWF AIMESQVETG 

       430        440        450        460        470        480 
TPFILYKDAC NAKSNQKNLG TISSSNLCTE IIQYTSPDEI AVCNLASIAL PKFVRQKVGS 

       490        500        510        520        530        540 
TDPKEKEFDH QFLFEITKLI TVNLNVIIDR NYYPVAEAKT SNLRHRPIGI GIQGLADVFI 

       550        560        570        580        590        600 
KMRMPFDSVA AAKLNVEIAE TIYFAALTAS HELAIRDGTY ESFKGSPASK GILQFDMWNV 

       610        620        630        640        650        660 
TPSSRWNWAE LKDNIVNKGG LRNSLLIAPM PTASTSQILG NNECFEPYTS NIYSRRVLAG 

       670        680        690        700        710        720 
EFTIVNKQLL EDLMELGIWS PEMKNQIVAA RGSIQSIGGI PDDLKELYKT VWEIRQRTLI 

       730        740        750        760        770        780 
DMAADRGAFI DQSQSFNVFI AEPTFAKLTS MHFYSWKKGL KTGMYYLRTR PAADAIQFTV 

       790        800        810        820        830        840 
DPIVSQTPKP VENNINSTTP LKTPTKTPNS SNRISTSPTN NLTSPIRFNI TQQQQQQQQQ 

       850        860        870 
QQQQNNNEDD LANYDGMTCR REEGCLVCGS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000063 Genomic DNA. Translation: EAL65376.1.
RefSeqXP_638726.1. XM_633634.1.

3D structure databases

ProteinModelPortalQ54Q71.
SMRQ54Q71. Positions 30-812.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230075.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230075; DDB0230075; DDB_G0284071.
GeneID8624396.
KEGGddi:DDB_G0284071.

Organism-specific databases

dictyBaseDDB_G0284071. rnrA.

Phylogenomic databases

eggNOGCOG0209.
OMAKECAMIS.
PhylomeDBQ54Q71.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

InterProIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
SUPFAMSSF48168. SSF48168. 1 hit.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
PROSITEPS51161. ATP_CONE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54Q71.

Entry information

Entry nameRIR1_DICDI
AccessionPrimary (citable) accession number: Q54Q71
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase