Q54Q04 (KYNU_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kynureninase
EC=3.7.1.3

Alternative name(s):
L-kynurenine hydrolase

Gene names

Name:	kynu
ORF Names:	DDB_G0284203

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively By similarity.
Catalytic activity	L-kynurenine + H2O = anthranilate + L-alanine. L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynureninase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Kynureninase		PRO_0000331482
Regions
	Region	159 – 162	4	Pyridoxal phosphate binding By similarity
	Coiled coil	8 – 36	29	Potential
Sites
	Binding site	131	1	Pyridoxal phosphate; via amide nitrogen By similarity
	Binding site	132	1	Pyridoxal phosphate By similarity
	Binding site	244	1	Pyridoxal phosphate By similarity
	Binding site	247	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
	Binding site	303	1	Pyridoxal phosphate By similarity
	Binding site	331	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	270	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54Q04 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 99A0FA2091907172	FASTA	451	50,799
	10 20 30 40 50 60 MDKFFEYIKN QNLSLEDEKL ADKLDQLDQL SSIKEEFYFP ITKDIATDLS RVKDEDLDKP 70 80 90 100 110 120 VIYLTGNSLG LQPKEIEKQL VCNYLNDWRK YGVEGHHKGD HPFIHIDEEI QASLSKIVGA 130 140 150 160 170 180 LPSEVCPMNS LSTNIHVLLS NFYKPTQTRH KIIIEYGAFP SDLYVTESQI RHNSFNPETS 190 200 210 220 230 240 LIKIKPRDGE YTLRTDDIIN VLKEHGDSVA VVMLSGIQYF TGQFFDMKKI TEVGHEIGAI 250 260 270 280 290 300 VGWDLAHAAG NVELSLHDWN VDFACWCTYK YLNSGPGCIA GIFVHSKHTE SFNLSTDSRL 310 320 330 340 350 360 LGWFGNKLSN RFQKEKEFVA EDGALGFRMS NPSVADCTAL RASLSVFEKA GGIKKLAEKS 370 380 390 400 410 420 TIITGYLEYL LTHKLKTTDV QIITPSEANQ RGSQLSLLIK GIKASQLKVN LSNSGIVCDV 430 440 450 RDPDVIRVAP APLYTSFNDV KYFIQKLNEN M

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000064 Genomic DNA. Translation: EAL65290.1.
RefSeq	XP_638641.1. XM_633549.1.
3D structure databases
ProteinModelPortal	Q54Q04.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54Q04.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231361; DDB0231361; DDB_G0284203.
GeneID	8624472.
GenomeReviews	Gene locus kynu in contig CM000153_GR.
KEGG	ddi:DDB_G0284203.
Organism-specific databases
dictyBase	DDB_G0284203. kynu.
Phylogenomic databases
eggNOG	KOG3846.
HOGENOM	HBG523016.
OMA	TAEAHKR.
PhylomeDB	Q54Q04.
ProtClustDB	CLSZ2430281.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR010111. Kynureninase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K01556.
PANTHER	PTHR14084. Kynureninase. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF038800. KYNU. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01814. Kynureninase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNU_DICDI

Accession

Primary (citable) accession number: Q54Q04

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)