Q54PV8 (IPYR_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa1
ORF Names:	DDB_G0284265

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from sequence or structural similarity. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.2. Source: dictyBase
Molecular function	inorganic diphosphatase activity Inferred from sequence or structural similarity. Source: dictyBase magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Inorganic pyrophosphatase		PRO_0000328312
Sites
	Metal binding	132	1	Magnesium 1 By similarity
	Metal binding	137	1	Magnesium 1 By similarity
	Metal binding	137	1	Magnesium 2 By similarity
	Metal binding	169	1	Magnesium 1 By similarity
	Binding site	100	1	Diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54PV8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 4C4776824D17B9AE	FASTA	279	31,441
	10 20 30 40 50 60 MKNFFSFSKL TPHLKNIYHR NMTYTTKQVG ETGSLEYRLF FLKDNKPVSS FHDVPLWVNK 70 80 90 100 110 120 EKQIVNMLVE IPRGTNAKLE IATKEYMNPI KQDVKDGKLR FVHDKYPFNY GALPQTWESP 130 140 150 160 170 180 EHTHPSTGAK GDNDPLDACE IGSGQGVTGE FKQVKVLGVF AMIDAGETDW KILCIDVNDP 190 200 210 220 230 240 IASQINSQED IEKHLPGKIN EVYTFLRDYK IPDGKGPNQF AFDGKLQSID FSMKIIEETE 250 260 270 AEWKDLVGGK TKSSLSVVNT TLNDSNTVTA DVAAQKLNF

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000064 Genomic DNA. Translation: EAL65321.1.
RefSeq	XP_638687.1. XM_633595.1.
3D structure databases
HSSP	HSSP built from PDB template 1WGI based on UniProtKB P00817.
ProteinModelPortal	Q54PV8.
SMR	Q54PV8. Positions 23-268.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54PV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233791; DDB0233791; DDB_G0284265.
GeneID	8624517.
GenomeReviews	Gene locus ppa1 in contig CM000153_GR.
KEGG	ddi:DDB_G0284265.
Organism-specific databases
dictyBase	DDB_G0284265. ppa1.
Phylogenomic databases
eggNOG	KOG1626.
GeneTree	EPrGT00050000004967.
HOGENOM	HBG434750.
OMA	WFFISGS.
PhylomeDB	Q54PV8.
ProtClustDB	CLSZ2728945.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_DICDI

Accession

Primary (citable) accession number: Q54PV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)