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Protein

Vacuolar protein sorting-associated protein 4

Gene

vps4

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in intracellular protein transport probably out of a prevacuolar endosomal compartment. May be involved in the release of components of the bilayered coat from the endosomal membrane. The association with ESCRT-III complex mediates the ATP-dependent disassembly of the ESCRT-III complex (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 1828ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. endosomal transport Source: dictyBase
  2. protein transport Source: UniProtKB-KW
  3. vacuole organization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 4 (EC:3.6.4.6)
Gene namesi
Name:vps4
ORF Names:DDB_G0284347
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284347. vps4.

Subcellular locationi

GO - Cellular componenti

  1. endosome Source: dictyBase
  2. late endosome membrane Source: UniProtKB-SubCell
  3. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Vacuolar protein sorting-associated protein 4PRO_0000331380Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0185960.

Structurei

3D structure databases

ProteinModelPortaliQ54PT2.
SMRiQ54PT2. Positions 124-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8079MITAdd
BLAST

Domaini

The MIT domain may serve as an adapter for the ESCRT-III complex.By similarity

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated
Contains 1 MIT domain.Curated

Phylogenomic databases

eggNOGiCOG0464.
InParanoidiQ54PT2.
KOiK12196.
OMAiAVGQTPC.
PhylomeDBiQ54PT2.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54PT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDVNFLQKA IQIVQQATEQ DNAKNYAEAH RLYIQSLEWF TTALKYEKSE
60 70 80 90 100
RSKATIKAKT LEYLQRAEQL KEYLDKSKNK KPVAVGGNKS NSAGSANGAG
110 120 130 140 150
KSAKEDDEDM DPEDKKRNDS LSSSIVTTKP NVKWDDVAGL YQAKEYLKEA
160 170 180 190 200
VIFPIKFPQM FTGNRKPWKG ILLYGPPGTG KSYLAKAVAT EISSTFFSIS
210 220 230 240 250
PSDIVTKWLG DSEKLVKQLF EMAREKNNSV IFIDEVDSLC SSRNDQESES
260 270 280 290 300
ARRIKTEFLI QMNGVGNDSD GILVLAATNI PWGLDLAIRR RFEKRIYIGL
310 320 330 340 350
PEPQARAKMF QIHIGSTPNT LVQADYKKLA DLTEGYSGSD IGSLVKDAIM
360 370 380 390 400
QPVRAVQCAT HFKQIRAPSR EDPSVMTDYV TPCSPGDPLA QEMTWMDIDP
410 420 430 440
TKLKEPEITI ADCLKSLRVI KPSVNKADLD RYVEFTNDFG QDGV
Length:444
Mass (Da):49,605
Last modified:May 23, 2005 - v1
Checksum:i764EAB9A43A34284
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000064 Genomic DNA. Translation: EAL65222.1.
RefSeqiXP_638572.1. XM_633480.1.

Genome annotation databases

EnsemblProtistsiDDB0234037; DDB0234037; DDB_G0284347.
GeneIDi8624544.
KEGGiddi:DDB_G0284347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000064 Genomic DNA. Translation: EAL65222.1.
RefSeqiXP_638572.1. XM_633480.1.

3D structure databases

ProteinModelPortaliQ54PT2.
SMRiQ54PT2. Positions 124-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0185960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0234037; DDB0234037; DDB_G0284347.
GeneIDi8624544.
KEGGiddi:DDB_G0284347.

Organism-specific databases

dictyBaseiDDB_G0284347. vps4.

Phylogenomic databases

eggNOGiCOG0464.
InParanoidiQ54PT2.
KOiK12196.
OMAiAVGQTPC.
PhylomeDBiQ54PT2.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiVPS4_DICDI
AccessioniPrimary (citable) accession number: Q54PT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 28, 2008
Last sequence update: May 23, 2005
Last modified: February 3, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.