Q54PM7 (NAGK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
Short name=N-acetylglucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	nagk
ORF Names:	DDB_G0284433

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, into GlcNAc 6-phosphate. Also has ManNAc kinase activity By similarity.
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
Sequence caution	The sequence EAL65265.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	N-acetyl-D-glucosamine kinase		PRO_0000331385
Regions
	Nucleotide binding	7 – 14	8	ATP By similarity
	Region	137 – 138	2	Substrate binding By similarity
	Region	153 – 155	3	Substrate binding By similarity
Sites
	Binding site	37	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54PM7 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: E5C017C4ED95F26F	FASTA	319	34,504
	10 20 30 40 50 60 MSKEIFIGID GGGTKTSTVA VDSNGQELAR HTSPCSNYHS VGEDLAKAAI NEGIKYVIRK 70 80 90 100 110 120 VKETITDDDN KEVTVGSICL GMSGVDREKD KLLVKSWVTE LLGESINYSI HNDAIVALSS 130 140 150 160 170 180 GTQGKLFGVV IICGTGCISL GFNREGVSGR SGGWGPLLGD YGSGYQIGYD ILRHVLKAKD 190 200 210 220 230 240 QVGPKTSLTQ VLLEKLQLTK EEDLISWAYD PKTQSWQKFA QLSPLAFEQA QLGDEISNLI 250 260 270 280 290 300 LVDAANALYD LINSVIKKLG LDKEEKFPLV YTGGNIERKG ILSDLLSKKI MENYPNAEIL 310 NTTCDPSMGA ALLALNSKK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000064 Genomic DNA. Translation: EAL65265.1. Different initiation.
RefSeq	XP_638627.1. XM_633535.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0186012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8624598.
KEGG	ddi:DDB_G0284433.
Organism-specific databases
dictyBase	DDB_G0284433.
Phylogenomic databases
eggNOG	COG2971.
ProtClustDB	CLSZ2430276.
Family and domain databases
InterPro	IPR002731. ATPase_BadF. [Graphical view]
Pfam	PF01869. BcrAD_BadFG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_DICDI

Accession

Primary (citable) accession number: Q54PM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)