Q54PA0 (MPI_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

Name:	mpi
ORF Names:	DDB_G0284685

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannose-6-phosphate isomerase activity Inferred from sequence or structural similarity. Source: dictyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Probable mannose-6-phosphate isomerase		PRO_0000327494
Regions
	Compositional bias	3 – 20	18	Poly-Asn
	Compositional bias	102 – 111	10	Poly-Ser
Sites
	Active site	314	1	By similarity
	Metal binding	141	1	Zinc By similarity
	Metal binding	143	1	Zinc By similarity
	Metal binding	168	1	Zinc By similarity
	Metal binding	295	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54PA0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: FBD259EF215E6D47	FASTA	452	51,091
	10 20 30 40 50 60 MENNNNNNNN NNNNNNNNNN IVLLKCVSQN YEWGKYGSNS TVAKLLKGYA KECSDIIKET 70 80 90 100 110 120 IPYAELWMGD HVSAPSKVEY KNKELKLREY IDTVQKEINE KSSTSSSSSS SIRGEIVEKR 130 140 150 160 170 180 FGNDFPFLFK VLSIRTALSI QAHPDSQLAQ VLFKKYPNIY KDPYHKPEIA IATTPFEALC 190 200 210 220 230 240 SFRPLSEIQS FIDTIPEFKN SLPNNLIKLD DCKEYLKSIV TSLLKADGLL ISNNLKELNN 250 260 270 280 290 300 RLNEKREEER DDLDRLVLKL YSQYPGDVGV FFAYILNYIV LKPGEALFLG AGEPHAYISG 310 320 330 340 350 360 DCVECMAPSD NVVRAGLTPK LKDVDTLGDM LTYRTGRPDL VVPQQRPDLS LTNYRCYQPP 370 380 390 400 410 420 VDEFQVEYYH LDDCCKNINV YSSKGPSIVL VYSGNLSIEN KSNQSTLNNL HTGSILFVPA 430 440 450 NTDYQFIQSD SSIPVSIYVA SVSNRIFNKN NL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000070 Genomic DNA. Translation: EAL65112.1.
RefSeq	XP_638475.1. XM_633383.1.
3D structure databases
ProteinModelPortal	Q54PA0.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231659.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231659; DDB0231659; DDB_G0284685.
GeneID	8624726.
KEGG	ddi:DDB_G0284685.
Organism-specific databases
dictyBase	DDB_G0284685. mpi.
Phylogenomic databases
eggNOG	COG1482.
KO	K01809.
OMA	EMTIAIT.
ProtClustDB	CLSZ2497174.
Enzyme and pathway databases
UniPathway	UPA00126; UER00423.
Family and domain databases
Gene3D	2.60.120.10. 3 hits.
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. False negative. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_DICDI

Accession

Primary (citable) accession number: Q54PA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)