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Protein

Probable mannose-6-phosphate isomerase

Gene

mpi

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable mannose-6-phosphate isomerase (mpi)
  2. Phosphomannomutase 1 (pmmA), Phosphomannomutase 2 (pmmB)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141ZincBy similarity1
Metal bindingi143ZincBy similarity1
Metal bindingi168ZincBy similarity1
Metal bindingi295ZincBy similarity1
Active sitei314By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-446205. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:mpi
ORF Names:DDB_G0284685
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284685. mpi.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003274941 – 452Probable mannose-6-phosphate isomeraseAdd BLAST452

Proteomic databases

PaxDbiQ54PA0.
PRIDEiQ54PA0.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231659.

Structurei

3D structure databases

ProteinModelPortaliQ54PA0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 20Poly-AsnAdd BLAST18
Compositional biasi102 – 111Poly-Ser10

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2757. Eukaryota.
COG1482. LUCA.
InParanoidiQ54PA0.
KOiK01809.
OMAiDDCKEYL.
PhylomeDBiQ54PA0.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 2 hits.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54PA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNNNNNN NNNNNNNNNN IVLLKCVSQN YEWGKYGSNS TVAKLLKGYA
60 70 80 90 100
KECSDIIKET IPYAELWMGD HVSAPSKVEY KNKELKLREY IDTVQKEINE
110 120 130 140 150
KSSTSSSSSS SIRGEIVEKR FGNDFPFLFK VLSIRTALSI QAHPDSQLAQ
160 170 180 190 200
VLFKKYPNIY KDPYHKPEIA IATTPFEALC SFRPLSEIQS FIDTIPEFKN
210 220 230 240 250
SLPNNLIKLD DCKEYLKSIV TSLLKADGLL ISNNLKELNN RLNEKREEER
260 270 280 290 300
DDLDRLVLKL YSQYPGDVGV FFAYILNYIV LKPGEALFLG AGEPHAYISG
310 320 330 340 350
DCVECMAPSD NVVRAGLTPK LKDVDTLGDM LTYRTGRPDL VVPQQRPDLS
360 370 380 390 400
LTNYRCYQPP VDEFQVEYYH LDDCCKNINV YSSKGPSIVL VYSGNLSIEN
410 420 430 440 450
KSNQSTLNNL HTGSILFVPA NTDYQFIQSD SSIPVSIYVA SVSNRIFNKN

NL
Length:452
Mass (Da):51,091
Last modified:May 24, 2005 - v1
Checksum:iFBD259EF215E6D47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000070 Genomic DNA. Translation: EAL65112.1.
RefSeqiXP_638475.1. XM_633383.1.

Genome annotation databases

EnsemblProtistsiEAL65112; EAL65112; DDB_G0284685.
GeneIDi8624726.
KEGGiddi:DDB_G0284685.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000070 Genomic DNA. Translation: EAL65112.1.
RefSeqiXP_638475.1. XM_633383.1.

3D structure databases

ProteinModelPortaliQ54PA0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231659.

Proteomic databases

PaxDbiQ54PA0.
PRIDEiQ54PA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65112; EAL65112; DDB_G0284685.
GeneIDi8624726.
KEGGiddi:DDB_G0284685.

Organism-specific databases

dictyBaseiDDB_G0284685. mpi.

Phylogenomic databases

eggNOGiKOG2757. Eukaryota.
COG1482. LUCA.
InParanoidiQ54PA0.
KOiK01809.
OMAiDDCKEYL.
PhylomeDBiQ54PA0.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
ReactomeiR-DDI-446205. Synthesis of GDP-mannose.

Miscellaneous databases

PROiQ54PA0.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 2 hits.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPI_DICDI
AccessioniPrimary (citable) accession number: Q54PA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.