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Protein

Porphobilinogen deaminase

Gene

hemC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.By similarity

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneBy similarityNote: Binds 1 dipyrromethane group covalently.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (alad)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

ReactomeiR-DDI-189451. Heme biosynthesis.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Alternative name(s):
Heme biosynthesis protein C
Hydroxymethylbilane synthase
Short name:
HMBS
Gene namesi
Name:hemC
ORF Names:DDB_G0284697
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284697. hemC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Porphobilinogen deaminasePRO_0000327798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei253 – 2531S-(dipyrrolylmethanemethyl)cysteineBy similarity

Proteomic databases

PaxDbiQ54P93.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231417.

Structurei

3D structure databases

ProteinModelPortaliQ54P93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiKOG2892. Eukaryota.
COG0181. LUCA.
InParanoidiQ54P93.
KOiK01749.
OMAiGIECRTD.
PhylomeDBiQ54P93.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54P93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSITKRDKV IIGSRKSQLA MLQTEWVRDR IQELNPGIIV EIKTMDTTGD
60 70 80 90 100
KVLDVSLSKI GDKGLFTKEL EDMMLNGTID LAVHSLKDIP TKLPDGLKLG
110 120 130 140 150
AITKRYNTSD AFIANAKKHG KNCKLSELPQ GAMIGSSSLR RVAQLKKAYP
160 170 180 190 200
HLQFKDIRGN LNTRFKKLED DSNGYDGMIL AVAGLERMEL TDHISEIIPD
210 220 230 240 250
SISLYAVGQG SLGIECKDGD DFIQSILNPL IHRESMYCCE AERSMLRDLE
260 270 280 290 300
GGCHVPIGVV TKLHNQSQPD ETLEINAIVL NLDGSKYIES KIIGPSIQYY
310 320
QLGKSIAQDL INKGSKDILS EFIKK
Length:325
Mass (Da):36,033
Last modified:May 24, 2005 - v1
Checksum:i2498FEF3ACA97A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000070 Genomic DNA. Translation: EAL65118.1.
RefSeqiXP_638482.1. XM_633390.1.

Genome annotation databases

EnsemblProtistsiDDB0231417; DDB0231417; DDB_G0284697.
GeneIDi8624733.
KEGGiddi:DDB_G0284697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000070 Genomic DNA. Translation: EAL65118.1.
RefSeqiXP_638482.1. XM_633390.1.

3D structure databases

ProteinModelPortaliQ54P93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231417.

Proteomic databases

PaxDbiQ54P93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231417; DDB0231417; DDB_G0284697.
GeneIDi8624733.
KEGGiddi:DDB_G0284697.

Organism-specific databases

dictyBaseiDDB_G0284697. hemC.

Phylogenomic databases

eggNOGiKOG2892. Eukaryota.
COG0181. LUCA.
InParanoidiQ54P93.
KOiK01749.
OMAiGIECRTD.
PhylomeDBiQ54P93.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00319.
ReactomeiR-DDI-189451. Heme biosynthesis.

Miscellaneous databases

PROiQ54P93.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam. 1 hit.
InterProiIPR000860. HemC.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM3_DICDI
AccessioniPrimary (citable) accession number: Q54P93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: December 9, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.