Q54P77 (4CL1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 4-coumarate--CoA ligase 1
Short name=4CL 1
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase 1

Gene names

Name:	4cl1
ORF Names:	DDB_G0284831

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Probable 4-coumarate--CoA ligase 1		PRO_0000327701

Sequences

	Sequence		Length	Mass (Da)
	Q54P77 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0F479EFDC4ACD3DE	FASTA	551	61,000
	10 20 30 40 50 60 MIIKVKGQTY FTSKYPNIII PEKPVPQLIL KHIRSKPDQV LLVDGLTFKE YSSHFVADTI 70 80 90 100 110 120 EKVACGLNKL NIKKGDVLGV ILPNLPEYVP IFHGTLLMGG ITSLVNPDYT IEELSHTLAT 130 140 150 160 170 180 VSPRYLAVTL AVYEKIKNDL KRVFPSVEKV ILVDIAGQTL KEIDQLTLSS DGIVMSFNQL 190 200 210 220 230 240 INNNGKDYPI VRIDPKKDTA IIPFSSGTTG LFKGVCLSHH NIVSNTYQTQ TIETSTYKKN 250 260 270 280 290 300 DTVMGILPFF HIYGLMLFLM LMVKQGHRVV VLPKFEPVRF LELIQKYKVA ISFIVPPVAI 310 320 330 340 350 360 MFAKSPIVDK FDLSSLRTLF SGAAPLSSEV EDLIKERFKG RLVIKQGYGA TELSPACFVI 370 380 390 400 410 420 PSGLVKSGSA GILLPNQLAK IISPETGENL GMGEKGEICI KGPNVMLGYY NNEKATNEVI 430 440 450 460 470 480 DKDGFLKTGD IGYVDEDGYY FIVDRSKELI KCKGFQVPPA ELEALLLSHP KVADACVVGL 490 500 510 520 530 540 SKGDMGEVPR GFVVIKQNES LTEKELLDWA HPKIANYKHF RGGIFFIPAI PKSATGKLLR 550 KNLKDFNPPK L

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000071 Genomic DNA. Translation: EAL65068.1.
RefSeq	XP_638381.1. XM_633289.1.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	Q54P77.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54P77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231737; DDB0231737; DDB_G0284831.
GeneID	8624750.
GenomeReviews	Gene locus 4cl1 in contig CM000153_GR.
KEGG	ddi:DDB_G0284831.
Organism-specific databases
dictyBase	DDB_G0284831. 4cl1.
Phylogenomic databases
eggNOG	KOG1176.
GeneTree	EPrGT00050000002817.
HOGENOM	HBG547964.
OMA	GRISENM.
PhylomeDB	Q54P77.
ProtClustDB	CLSZ2430218.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL1_DICDI

Accession

Primary (citable) accession number: Q54P77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)