Reviewed, UniProtKB/Swiss-Prot Q54P77 (4CL1_DICDI)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 1
      Short name=4CL 1
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 1

Gene names

Name:	4cl1
ORF Names:	DDB_G0284831

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Probable 4-coumarate--CoA ligase 1		PRO_0000327701

Sequences

	Sequence		Length	Mass (Da)
	Q54P77-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0F479EFDC4ACD3DE	FASTA	551	61,000
	10 20 30 40 50 60 MIIKVKGQTY FTSKYPNIII PEKPVPQLIL KHIRSKPDQV LLVDGLTFKE YSSHFVADTI 70 80 90 100 110 120 EKVACGLNKL NIKKGDVLGV ILPNLPEYVP IFHGTLLMGG ITSLVNPDYT IEELSHTLAT 130 140 150 160 170 180 VSPRYLAVTL AVYEKIKNDL KRVFPSVEKV ILVDIAGQTL KEIDQLTLSS DGIVMSFNQL 190 200 210 220 230 240 INNNGKDYPI VRIDPKKDTA IIPFSSGTTG LFKGVCLSHH NIVSNTYQTQ TIETSTYKKN 250 260 270 280 290 300 DTVMGILPFF HIYGLMLFLM LMVKQGHRVV VLPKFEPVRF LELIQKYKVA ISFIVPPVAI 310 320 330 340 350 360 MFAKSPIVDK FDLSSLRTLF SGAAPLSSEV EDLIKERFKG RLVIKQGYGA TELSPACFVI 370 380 390 400 410 420 PSGLVKSGSA GILLPNQLAK IISPETGENL GMGEKGEICI KGPNVMLGYY NNEKATNEVI 430 440 450 460 470 480 DKDGFLKTGD IGYVDEDGYY FIVDRSKELI KCKGFQVPPA ELEALLLSHP KVADACVVGL 490 500 510 520 530 540 SKGDMGEVPR GFVVIKQNES LTEKELLDWA HPKIANYKHF RGGIFFIPAI PKSATGKLLR 550 KNLKDFNPPK L

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000071 Genomic DNA. Translation: EAL65068.1.
RefSeq	XP_638381.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3389964.
GenomeReviews	Gene locus 4cl1 in contig CM000153_GR.
KEGG	ddi:DDB_0231737.
Organism-specific databases
dictyBase	DDB_G0284831. 4cl1.
Phylogenomic databases
OMA	ECSMASH.
Enzyme and pathway databases
BRENDA	6.2.1.12. 424.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL1_DICDI

Accession

Primary (citable) accession number: Q54P77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)