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Q54NU9 (TPSB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B

EC=2.4.1.15
Alternative name(s):
Trehalose-6-phosphate synthase B
UDP-glucose-glucosephosphate glucosyltransferase B
Gene names
Name:tpsB
ORF Names:DDB_G0284975
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length790 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination. Ref.2

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 20 family.

In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 790790Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B
PRO_0000328411

Sequences

Sequence LengthMass (Da)Tools
Q54NU9 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: EA8E23A53F6DADB7

FASTA79090,775
        10         20         30         40         50         60 
MTIIQRSTSL NNIINEKLGK IIVASNTLPI TVTKFNETPL FGSPLSASRE SITSSFGMSE 

        70         80         90        100        110        120 
PSRDRESKIQ IQINGHPFPT QSALETLKAK DEIEDWLWIG WSHCEVNEDE EPMLNQAIKE 

       130        140        150        160        170        180 
FSPHFEHVFL NPRQFENYYK GYCKNGLWLL LHYQMNFIRM QSEWWEEYVG VNQMFAEKIA 

       190        200        210        220        230        240 
SVWRPSDIIW IHDYHLMLVP QMLRQLLPPE ASIGFFFHAP FPSYELFRIL PNRKELLKGI 

       250        260        270        280        290        300 
LSSNLIGFQS FEYVRHFKSS CARLLDLEVH PKGLEIFEDG STHFTKLQVY PIGVDYNDFA 

       310        320        330        340        350        360 
KNLNLPEVSS RVESLRKIFK GKKVVVARDR LDQIEGVPRK LEVFEQLLND HPEYIGKLVF 

       370        380        390        400        410        420 
IQIYEPTVEE GDETDEQKIL HKTVNEMVGR INGKFGKLSF NPIEYINKKI SYEELSALYK 

       430        440        450        460        470        480 
LADIALITPI RDGMNLTSHE YVVCQKDNFG VLILSEFAGA ARCLGGSIIV NPFSKKEIME 

       490        500        510        520        530        540 
AIIEALNMSM HDRKLKHQIN YNYVLANTSS FWGKRFLCDL NEATQKEIME TSVPRANFQE 

       550        560        570        580        590        600 
IEDSYKKAKV RVFFLDYDGT LTPLVRLPSQ AMPSKQLIDV LSKLTEDRRN EVYVISGRDR 

       610        620        630        640        650        660 
SSLEKWLGHL PIGMSCEHGV FTRQPGENQP WTESPNAEVQ WKDTVLSIMQ DFEDRTPGSM 

       670        680        690        700        710        720 
TETKQVNITW HYRNADPDFG QFQAKELIAQ LRSVANKYPL DILSGKKAIE VKPIGINKGE 

       730        740        750        760        770        780 
IVKMILQKID ADFILCIGDD KTDEDMFKAL YNVPSFTIRV CGDLEESTKA RGVVESSSEV 

       790 
LTLLNRLSLS 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps."
Killick K.A.
Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000073 Genomic DNA. Translation: EAL64951.1.

3D structure databases

ProteinModelPortalQ54NU9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231987.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231987; DDB0231987; DDB_G0284975.
KEGGddi:DDB_G0284975.

Organism-specific databases

dictyBaseDDB_G0284975. tpsB.

Phylogenomic databases

eggNOGCOG0380.
KOK16055.
OMAWHYEEAD.
PhylomeDBQ54NU9.
ProtClustDBCLSZ2728860.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
InterProIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPSB_DICDI
AccessionPrimary (citable) accession number: Q54NU9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase