Q54NU9 (TPSB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase B
UDP-glucose-glucosephosphate glucosyltransferase B

Gene names

Name:	tpsB
ORF Names:	DDB_G0284975

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination. Ref.2
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 790	790	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B		PRO_0000328411

Sequences

	Sequence		Length	Mass (Da)
	Q54NU9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: EA8E23A53F6DADB7	FASTA	790	90,775
	10 20 30 40 50 60 MTIIQRSTSL NNIINEKLGK IIVASNTLPI TVTKFNETPL FGSPLSASRE SITSSFGMSE 70 80 90 100 110 120 PSRDRESKIQ IQINGHPFPT QSALETLKAK DEIEDWLWIG WSHCEVNEDE EPMLNQAIKE 130 140 150 160 170 180 FSPHFEHVFL NPRQFENYYK GYCKNGLWLL LHYQMNFIRM QSEWWEEYVG VNQMFAEKIA 190 200 210 220 230 240 SVWRPSDIIW IHDYHLMLVP QMLRQLLPPE ASIGFFFHAP FPSYELFRIL PNRKELLKGI 250 260 270 280 290 300 LSSNLIGFQS FEYVRHFKSS CARLLDLEVH PKGLEIFEDG STHFTKLQVY PIGVDYNDFA 310 320 330 340 350 360 KNLNLPEVSS RVESLRKIFK GKKVVVARDR LDQIEGVPRK LEVFEQLLND HPEYIGKLVF 370 380 390 400 410 420 IQIYEPTVEE GDETDEQKIL HKTVNEMVGR INGKFGKLSF NPIEYINKKI SYEELSALYK 430 440 450 460 470 480 LADIALITPI RDGMNLTSHE YVVCQKDNFG VLILSEFAGA ARCLGGSIIV NPFSKKEIME 490 500 510 520 530 540 AIIEALNMSM HDRKLKHQIN YNYVLANTSS FWGKRFLCDL NEATQKEIME TSVPRANFQE 550 560 570 580 590 600 IEDSYKKAKV RVFFLDYDGT LTPLVRLPSQ AMPSKQLIDV LSKLTEDRRN EVYVISGRDR 610 620 630 640 650 660 SSLEKWLGHL PIGMSCEHGV FTRQPGENQP WTESPNAEVQ WKDTVLSIMQ DFEDRTPGSM 670 680 690 700 710 720 TETKQVNITW HYRNADPDFG QFQAKELIAQ LRSVANKYPL DILSGKKAIE VKPIGINKGE 730 740 750 760 770 780 IVKMILQKID ADFILCIGDD KTDEDMFKAL YNVPSFTIRV CGDLEESTKA RGVVESSSEV 790 LTLLNRLSLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps." Killick K.A. Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000073 Genomic DNA. Translation: EAL64951.1.
RefSeq	XP_639968.1. XM_634876.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortal	Q54NU9.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231987.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231987; DDB0231987; DDB_G0284975.
GeneID	8624879.
KEGG	ddi:DDB_G0284975.
Organism-specific databases
dictyBase	DDB_G0284975. tpsB.
Phylogenomic databases
eggNOG	COG0380.
KO	K16055.
OMA	IIVANRL.
ProtClustDB	CLSZ2728860.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR00685. T6PP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPSB_DICDI

Accession

Primary (citable) accession number: Q54NU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)