Reviewed, UniProtKB/Swiss-Prot Q54NM9 (STT3_DICDI)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
      Short name=Oligosaccharyl transferase subunit STT3
    EC=2.4.1.119

Gene names

Name:	stt3
ORF Names:	DDB_G0285159

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Sst3 seems to be involved in complex substrate specificity By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the STT3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3		PRO_0000328633
Regions
	Transmembrane	40 – 60	21	Potential
	Transmembrane	107 – 127	21	Potential
	Transmembrane	139 – 159	21	Potential
	Transmembrane	167 – 187	21	Potential
	Transmembrane	188 – 208	21	Potential
	Transmembrane	210 – 230	21	Potential
	Transmembrane	232 – 252	21	Potential
	Transmembrane	261 – 281	21	Potential
	Transmembrane	290 – 310	21	Potential
	Transmembrane	327 – 347	21	Potential
	Transmembrane	383 – 403	21	Potential
	Transmembrane	408 – 428	21	Potential
	Transmembrane	430 – 450	21	Potential
	Transmembrane	474 – 494	21	Potential
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	467	1	N-linked (GlcNAc...) Potential
	Glycosylation	553	1	N-linked (GlcNAc...) Potential
	Glycosylation	560	1	N-linked (GlcNAc...) Potential
	Glycosylation	564	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54NM9-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 5C21BBCEC770B656	FASTA	714	80,546
	10 20 30 40 50 60 MKRSEKSSTS VVSNNKQQDV NIISSNEVGV KEENKGHQEF LLKVLILSVI YVLAFSTRLF 70 80 90 100 110 120 SVLRYESVIH EFDPYFNYRS TIYLVQEGFY NFLNWFDERA WYPLGRIVGG TIYPGLMATA 130 140 150 160 170 180 SLVHWSLNSL NITVNIRNVC VLLSPWFASN TAMVTYKFAK EVKDTQTGLV AAAMIAIVPG 190 200 210 220 230 240 YISRSVAGSF DNEGIAIFAL IFTYYCWIKS VNTGSLMWAA ICSLAYFYMA SAWGGYVFII 250 260 270 280 290 300 NLIPLHAFFL LLTGRYSHRL YIAYSTMFVI GTILSMQITF ISFQPVQSSE HLAAIGIFGL 310 320 330 340 350 360 LQLYAGLSWV KSHLTNEAFK KLQRLTVLFV LSCAAAVLVV GTLTGYISPF NGRFYSLLDP 370 380 390 400 410 420 TYARDHIPII ASVSEHQPTT WASYFFDLHI LVFLFPAGLY FCFQKLTDAN IFLILYGVTS 430 440 450 460 470 480 IYFSGVMVRL MLVLAPVACI LAAVAVSATL TTYMKKLKAP SSPSDANNSK ESGGVMVAVL 490 500 510 520 530 540 TVLLILYAFH CTWVTSEAYS SPSIVLSAKQ NDGSRVIFDD FREAYRWIGQ NTADDARIMS 550 560 570 580 590 600 WWDYGYQLSA MANRTVLVDN NTWNNSHIAQ VGKAFASTEE DAYIQMKALD VDYVLVIFGG 610 620 630 640 650 660 LTGYSSDDIN KFLWMVRIGG SCDPNIKEQD YLTNGQYRID KGASPTMLNS LMYKLSYYRF 670 680 690 700 710 SEVHTDYQRP TGFDRVRNVE IGNKNFDLTY LEEAFTSVHW LVRVYKVKDF DNRA

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000074 Genomic DNA. Translation: EAL64892.1.
RefSeq	XP_639887.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54NM9.
Genome annotation databases
GeneID	3390975.
GenomeReviews	Gene locus stt3 in contig CM000153_GR.
KEGG	ddi:DDB_0230191.
Organism-specific databases
dictyBase	DDB_G0285159. stt3.
Phylogenomic databases
OMA	NIFLILY.
Enzyme and pathway databases
BRENDA	2.4.1.119. 424.
Family and domain databases
InterPro	IPR003674. Oligo_trans_STT3. [Graphical view]
Pfam	PF02516. STT3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STT3_DICDI

Accession

Primary (citable) accession number: Q54NM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)