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Protein

Probable saccharopine dehydrogenase [NADP(+), L-glutamate-forming]

Gene

sdh

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH.

Pathwayi

GO - Molecular functioni

  1. saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. lysine biosynthetic process via aminoadipic acid Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00033; UER00033.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable saccharopine dehydrogenase [NADP(+), L-glutamate-forming] (EC:1.5.1.10)
Alternative name(s):
Saccharopine reductase
Gene namesi
Name:sdh
ORF Names:DDB_G0285267
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285267. sdh.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Probable saccharopine dehydrogenase [NADP(+), L-glutamate-forming]PRO_0000365593Add
BLAST

Proteomic databases

PRIDEiQ54NG9.

Expressioni

Developmental stagei

Up-regulated in aspidocytes, environmentally resistant cell types.1 Publication

Inductioni

Down-regulated by phagocytic stimuli.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0186419.

Structurei

3D structure databases

ProteinModelPortaliQ54NG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the saccharopine dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1748.
InParanoidiQ54NG9.
KOiK00293.
OMAiWNYKFTW.
PhylomeDBiQ54NG9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR005097. Saccharopine_DH/HSpermid_syn.
[Graphical view]
PfamiPF03435. Saccharop_dh. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54NG9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNILLLGSG FVAKPALDYL LKREDYFVTI VSLFQNELDS ITKGHDTSKF
60 70 80 90 100
KTIQLDVMNK LNELEEYFPK SDCVISLIPA TLHSTVAKLC IKHKTHLVTA
110 120 130 140 150
SYISDDMKAL SEEAKEAGVL LLNELGLDPG IDHMSSMKII DHAKENGGKV
160 170 180 190 200
TSFVSWCGAL PSTECADNPF GYKFSWSPRG VLSSATLSAN FLWEGHNEEV
210 220 230 240 250
PANIKWAVLQ PIVVEDSNGV KMEFDGVPNR NSFPYIEQYN LNAKDVTTMF
260 270 280 290 300
RGTLRWKGGF GIMIRALVAV GLFSTEVDAR LAVEGGISWR NYLVQLLGCN
310 320 330 340 350
DNDSDLLYCV ESTIKEYFEK LKTERDGLQF HFPIIPRDIE KDVQHAVEGF
360 370 380 390 400
KWLGLLSADE KVVNKNTPID SLCALLEKKL SYKAGERDVV VLEHNFVVQY
410 420 430 440 450
ADRTEKEVSS LICYGIPNGS SATSLTVGVP VGIATELIAD GKTTTRGVVG
460 470 480
PVTPEFYLPI LEKLKSENIE MIETKQEIKK
Length:480
Mass (Da):53,359
Last modified:May 24, 2005 - v1
Checksum:i10EE7E6746F7F894
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000077 Genomic DNA. Translation: EAL64797.1.
RefSeqiXP_638300.1. XM_633208.1.

Genome annotation databases

EnsemblProtistsiDDB0238270; DDB0238270; DDB_G0285267.
GeneIDi8625017.
KEGGiddi:DDB_G0285267.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000077 Genomic DNA. Translation: EAL64797.1.
RefSeqiXP_638300.1. XM_633208.1.

3D structure databases

ProteinModelPortaliQ54NG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0186419.

Proteomic databases

PRIDEiQ54NG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0238270; DDB0238270; DDB_G0285267.
GeneIDi8625017.
KEGGiddi:DDB_G0285267.

Organism-specific databases

dictyBaseiDDB_G0285267. sdh.

Phylogenomic databases

eggNOGiCOG1748.
InParanoidiQ54NG9.
KOiK00293.
OMAiWNYKFTW.
PhylomeDBiQ54NG9.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00033.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR005097. Saccharopine_DH/HSpermid_syn.
[Graphical view]
PfamiPF03435. Saccharop_dh. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "A new environmentally resistant cell type from Dictyostelium."
    Serafimidis I., Bloomfield G., Skelton J., Ivens A., Kay R.R.
    Microbiology 153:619-630(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  3. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
    Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
    BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCPDH_DICDI
AccessioniPrimary (citable) accession number: Q54NG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 24, 2005
Last modified: February 4, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.