Q54NG9 (SCPDH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable saccharopine dehydrogenase [NADP(+), L-glutamate-forming]
EC=1.5.1.10

Alternative name(s):
Saccharopine reductase

Gene names

Name:	sdh
ORF Names:	DDB_G0285267

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-glutamate + (S)-2-amino-6-oxohexanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.
Developmental stage	Up-regulated in aspidocytes, environmentally resistant cell types. Ref.2
Induction	Down-regulated by phagocytic stimuli.
Sequence similarities	Belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via aminoadipic acid Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from sequence or structural similarity. Source: dictyBase saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Probable saccharopine dehydrogenase [NADP(+), L-glutamate-forming]		PRO_0000365593

Sequences

	Sequence		Length	Mass (Da)
	Q54NG9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 10EE7E6746F7F894	FASTA	480	53,359
	10 20 30 40 50 60 MKNILLLGSG FVAKPALDYL LKREDYFVTI VSLFQNELDS ITKGHDTSKF KTIQLDVMNK 70 80 90 100 110 120 LNELEEYFPK SDCVISLIPA TLHSTVAKLC IKHKTHLVTA SYISDDMKAL SEEAKEAGVL 130 140 150 160 170 180 LLNELGLDPG IDHMSSMKII DHAKENGGKV TSFVSWCGAL PSTECADNPF GYKFSWSPRG 190 200 210 220 230 240 VLSSATLSAN FLWEGHNEEV PANIKWAVLQ PIVVEDSNGV KMEFDGVPNR NSFPYIEQYN 250 260 270 280 290 300 LNAKDVTTMF RGTLRWKGGF GIMIRALVAV GLFSTEVDAR LAVEGGISWR NYLVQLLGCN 310 320 330 340 350 360 DNDSDLLYCV ESTIKEYFEK LKTERDGLQF HFPIIPRDIE KDVQHAVEGF KWLGLLSADE 370 380 390 400 410 420 KVVNKNTPID SLCALLEKKL SYKAGERDVV VLEHNFVVQY ADRTEKEVSS LICYGIPNGS 430 440 450 460 470 480 SATSLTVGVP VGIATELIAD GKTTTRGVVG PVTPEFYLPI LEKLKSENIE MIETKQEIKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"A new environmentally resistant cell type from Dictyostelium." Serafimidis I., Bloomfield G., Skelton J., Ivens A., Kay R.R. Microbiology 153:619-630(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[3]	"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium." Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S. BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000077 Genomic DNA. Translation: EAL64797.1.
RefSeq	XP_638300.1. XM_633208.1.
3D structure databases
HSSP	HSSP built from PDB template 1E5Q based on UniProtKB Q9P4R4.
ProteinModelPortal	Q54NG9.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0186419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0238270; DDB0238270; DDB_G0285267.
GeneID	8625017.
KEGG	ddi:DDB_G0285267.
Organism-specific databases
dictyBase	DDB_G0285267. sdh.
Phylogenomic databases
eggNOG	COG1748.
KO	K00293.
OMA	VTVACRT.
Enzyme and pathway databases
UniPathway	UPA00033; UER00033.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR016040. NAD(P)-bd_dom. IPR005097. Saccharopine_DH/HSpermid_syn. [Graphical view]
Pfam	PF03435. Saccharop_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCPDH_DICDI

Accession

Primary (citable) accession number: Q54NG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)