Q54NE6 (PGAM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoglycerate mutase
EC=3.1.3.13
EC=5.4.2.1
EC=5.4.2.4

Alternative name(s):
BPG-dependent PGAM
Short name=dPGM

Gene names

Name:	gpmA
Synonyms:	pgam
ORF Names:	DDB_G0285311

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate By similarity.
Catalytic activity	2-phospho-D-glycerate = 3-phospho-D-glycerate. 3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate. 2,3-bisphospho-D-glycerate + H2O = 3-phospho-D-glycerate + phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Molecular function	Hydrolase Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from sequence or structural similarity. Source: dictyBase glycolysis Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytosol Inferred from sequence or structural similarity. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.2. Source: dictyBase
Molecular function	bisphosphoglycerate 2-phosphatase activity Inferred from electronic annotation. Source: EC bisphosphoglycerate mutase activity Inferred from electronic annotation. Source: EC phosphoglycerate mutase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Probable phosphoglycerate mutase		PRO_0000328074
Sites
	Active site	10	1	Tele-phosphohistidine intermediate By similarity
	Active site	183	1	By similarity
	Site	61	1	Interaction with carboxyl group of phosphoglycerates By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54NE6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: A1C3FA1FA891F37E	FASTA	249	28,480
	10 20 30 40 50 60 MVYKLVLIRH GESTWNKENK FTGWTDVDLS EKGVQEAHEA GKRLLKAGFT FDIAYTSVLK 70 80 90 100 110 120 RAIRTLWILL EELNLYWIPV SRQWRLNERM YGSLQGLNKS ETAAKYGEDQ VLIWRRSYDI 130 140 150 160 170 180 PPPALEESDE RYPGNDPRYA KLDKSDLPKT ECLKDTVERF LPLWNDTIAP TIKSGQKVLI 190 200 210 220 230 240 AAHGNSIRAL VKYLDNIADD KIVSMDIPTG IPLVYELDEN LKPIKHYYLA DESELNAAIQ AVANQGKAK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000078 Genomic DNA. Translation: EAL64785.1.
RefSeq	XP_638289.1. XM_633197.1.
3D structure databases
HSSP	HSSP built from PDB template 1E58 based on UniProtKB P62707.
ProteinModelPortal	Q54NE6.
SMR	Q54NE6. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54NE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231354; DDB0231354; DDB_G0285311.
GeneID	8625042.
GenomeReviews	Gene locus gpmA in contig CM000153_GR.
KEGG	ddi:DDB_G0285311.
Organism-specific databases
dictyBase	DDB_G0285311. gpmA.
Phylogenomic databases
eggNOG	KOG0235.
GeneTree	EPrGT00050000006067.
HOGENOM	HBG658938.
OMA	YWNDVII.
PhylomeDB	Q54NE6.
ProtClustDB	PTZ00123.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. IPR001345. PG/BPGM_mutase_AS. IPR005952. Phosphogly_mut1. [Graphical view]
KO	K01834.
PANTHER	PTHR11931. Phosphogly_mut1. 1 hit.
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01258. Pgm_1. 1 hit.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGAM_DICDI

Accession

Primary (citable) accession number: Q54NE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)