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Protein

Probable phosphoglycerate mutase

Gene

gpmA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.By similarity

Catalytic activityi

2-phospho-D-glycerate = 3-phospho-D-glycerate.
3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate.
2,3-bisphospho-D-glycerate + H2O = 3-phospho-D-glycerate + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101Tele-phosphohistidine intermediateBy similarity
Binding sitei61 – 611SubstrateBy similarity
Active sitei88 – 881Proton donor/acceptorBy similarity
Binding sitei99 – 991SubstrateBy similarity
Sitei183 – 1831Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Isomerase

Keywords - Biological processi

Glycolysis

Enzyme and pathway databases

BRENDAi3.1.3.80. 1939.
ReactomeiR-DDI-70171. Glycolysis.
R-DDI-70263. Gluconeogenesis.
R-DDI-70326. Glucose metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoglycerate mutase (EC:3.1.3.13, EC:5.4.2.11, EC:5.4.2.4)
Alternative name(s):
BPG-dependent PGAM
Short name:
dPGM
Gene namesi
Name:gpmA
Synonyms:pgam
ORF Names:DDB_G0285311
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285311. gpmA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Probable phosphoglycerate mutasePRO_0000328074Add
BLAST

Proteomic databases

PaxDbiQ54NE6.
PRIDEiQ54NE6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231354.

Structurei

3D structure databases

ProteinModelPortaliQ54NE6.
SMRiQ54NE6. Positions 2-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 168Substrate bindingBy similarity
Regioni22 – 232Substrate bindingBy similarity
Regioni88 – 914Substrate bindingBy similarity
Regioni115 – 1162Substrate bindingBy similarity
Regioni184 – 1852Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0235. Eukaryota.
COG0588. LUCA.
InParanoidiQ54NE6.
KOiK01834.
OMAiVKNQGKK.
PhylomeDBiQ54NE6.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54NE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYKLVLIRH GESTWNKENK FTGWTDVDLS EKGVQEAHEA GKRLLKAGFT
60 70 80 90 100
FDIAYTSVLK RAIRTLWILL EELNLYWIPV SRQWRLNERM YGSLQGLNKS
110 120 130 140 150
ETAAKYGEDQ VLIWRRSYDI PPPALEESDE RYPGNDPRYA KLDKSDLPKT
160 170 180 190 200
ECLKDTVERF LPLWNDTIAP TIKSGQKVLI AAHGNSIRAL VKYLDNIADD
210 220 230 240
KIVSMDIPTG IPLVYELDEN LKPIKHYYLA DESELNAAIQ AVANQGKAK
Length:249
Mass (Da):28,480
Last modified:May 24, 2005 - v1
Checksum:iA1C3FA1FA891F37E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000078 Genomic DNA. Translation: EAL64785.1.
RefSeqiXP_638289.1. XM_633197.1.

Genome annotation databases

EnsemblProtistsiDDB0231354; DDB0231354; DDB_G0285311.
GeneIDi8625042.
KEGGiddi:DDB_G0285311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000078 Genomic DNA. Translation: EAL64785.1.
RefSeqiXP_638289.1. XM_633197.1.

3D structure databases

ProteinModelPortaliQ54NE6.
SMRiQ54NE6. Positions 2-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231354.

Proteomic databases

PaxDbiQ54NE6.
PRIDEiQ54NE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231354; DDB0231354; DDB_G0285311.
GeneIDi8625042.
KEGGiddi:DDB_G0285311.

Organism-specific databases

dictyBaseiDDB_G0285311. gpmA.

Phylogenomic databases

eggNOGiKOG0235. Eukaryota.
COG0588. LUCA.
InParanoidiQ54NE6.
KOiK01834.
OMAiVKNQGKK.
PhylomeDBiQ54NE6.

Enzyme and pathway databases

BRENDAi3.1.3.80. 1939.
ReactomeiR-DDI-70171. Glycolysis.
R-DDI-70263. Gluconeogenesis.
R-DDI-70326. Glucose metabolism.

Miscellaneous databases

PROiQ54NE6.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
HAMAPiMF_01039. PGAM_GpmA.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR005952. Phosphogly_mut1.
[Graphical view]
PANTHERiPTHR11931. PTHR11931. 1 hit.
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR01258. pgm_1. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiPGAM_DICDI
AccessioniPrimary (citable) accession number: Q54NE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.