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Q54N83

- SYLC_DICDI

UniProt

Q54N83 - SYLC_DICDI

Protein

Leucine--tRNA ligase, cytoplasmic

Gene

leuS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei714 – 7141ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: dictyBase

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: dictyBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:leuS
    ORF Names:DDB_G0285451
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0285451. leuS.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10581058Leucine--tRNA ligase, cytoplasmicPRO_0000328291Add
    BLAST

    Proteomic databases

    PRIDEiQ54N83.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231253.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54N83.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionBy similarityAdd
    BLAST
    Motifi711 – 7155"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiFDENVAN.
    PhylomeDBiQ54N83.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54N83-1 [UniParc]FASTAAdd to Basket

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    MSTAKLDFVR AYEKEVQNKW ESDKQFEIDA LDAPDAEHPK YLATFPYPYM     50
    NGRLHVGHVF TITKAEFMCQ FQRLMGKRVL FPFAFHCTGM PIKACADKLK 100
    KEIEQFGCPP VFPVIEEKPI QEVTTAVKED PLSFKSKKTK AVAKSGGAVY 150
    QWKIMQSLGI SDEEIPMFAD SAYWLNYFPP HCESDLKLLG VGVDWRRSFI 200
    TTDVNGYYDS FVRWQFESLK ALGKVKYGKR YSIWSTIDDQ QCADHERAQG 250
    EGVGPQNYTL IKLEVVEPIP ECLKEIHSQG KKIYLVPGTL RPETMYGQTN 300
    CWILPTGKYG AFEMANGDIF VCTERSARNM SYQQMTTGKG EYKCLAKFEG 350
    SDILGAALKA PLAINPIVYV LPMLTIDENK GTGVVTSVPS DSPDDYASLQ 400
    DLKAKAPLRA KFSIKDEWVL PFEVVPIIDI PGYSNESAVR AYKDLGIKSQ 450
    NDRALLDQAK DLCYQKGFND GVMSVGPYAG RKVSEVKKII KDEMVASGQA 500
    VDYSEPTSKV VSRSGDECVV ALSDQWYINY GDDDIEWKNQ TIKQLESMEF 550
    YSAETKKKFE IALGWMNQWA CSRSFGLGTH LPWDEKFLIE SLSDSTIYMA 600
    FYTVAHLLQG DVNGSKPGSA GITPKQMTSA CWDYVLMGKP YPEGCEVSEE 650
    KLKELKKEFT YWYPVDIRVS GADLIQNHLT FFLYTHAAIF EKKFQPKSIR 700
    ANGFVNLNGE KMSKSTGNFL TLVDSVAKFS ADGTRVALAD AGDSIEDANF 750
    VDQTAVTSLL KLHTQIQWVQ ETLDSIDKFR SGPLDRIQDT IFESEINNII 800
    VESEKAYQKT NFRDALHLVF FDLQNARDHY KVTTLDQMHK DLVLRFIEVQ 850
    ALLIYPIAPH FAQKLFNMLG KGNILSAGWP KAGAIDYEAL KKNNYVQQTI 900
    YNFRMKLQVY QKAKLKGKPA GTKAIPDQST IIVSKSYPKW QQDVLEYLAT 950
    IYNEDSKSFT KDNNAIAEEL LSREEMKPHK KNLMGFVASA IQNVKESGKD 1000
    ALQTSLSFDE TSTLTDNIDY ICKTLELTSF DVKDFNEIPQ ASQSKIQPIP 1050
    GKPQFQFV 1058
    Length:1,058
    Mass (Da):119,529
    Last modified:May 24, 2005 - v1
    Checksum:i62D8D2139322198E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64563.1.
    RefSeqiXP_638060.1. XM_632968.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231253; DDB0231253; DDB_G0285451.
    GeneIDi8625106.
    KEGGiddi:DDB_G0285451.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64563.1 .
    RefSeqi XP_638060.1. XM_632968.1.

    3D structure databases

    ProteinModelPortali Q54N83.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231253.

    Proteomic databases

    PRIDEi Q54N83.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231253 ; DDB0231253 ; DDB_G0285451 .
    GeneIDi 8625106.
    KEGGi ddi:DDB_G0285451.

    Organism-specific databases

    dictyBasei DDB_G0285451. leuS.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi FDENVAN.
    PhylomeDBi Q54N83.

    Miscellaneous databases

    PROi Q54N83.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiSYLC_DICDI
    AccessioniPrimary (citable) accession number: Q54N83
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3