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Protein

Leucine--tRNA ligase, cytoplasmic

Gene

leuS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei714 – 7141ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
ORF Names:DDB_G0285451
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285451. leuS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Leucine--tRNA ligase, cytoplasmicPRO_0000328291Add
BLAST

Proteomic databases

PRIDEiQ54N83.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231253.

Structurei

3D structure databases

ProteinModelPortaliQ54N83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionBy similarityAdd
BLAST
Motifi711 – 7155"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ54N83.
KOiK01869.
OMAiSEHFPKT.
PhylomeDBiQ54N83.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54N83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAKLDFVR AYEKEVQNKW ESDKQFEIDA LDAPDAEHPK YLATFPYPYM
60 70 80 90 100
NGRLHVGHVF TITKAEFMCQ FQRLMGKRVL FPFAFHCTGM PIKACADKLK
110 120 130 140 150
KEIEQFGCPP VFPVIEEKPI QEVTTAVKED PLSFKSKKTK AVAKSGGAVY
160 170 180 190 200
QWKIMQSLGI SDEEIPMFAD SAYWLNYFPP HCESDLKLLG VGVDWRRSFI
210 220 230 240 250
TTDVNGYYDS FVRWQFESLK ALGKVKYGKR YSIWSTIDDQ QCADHERAQG
260 270 280 290 300
EGVGPQNYTL IKLEVVEPIP ECLKEIHSQG KKIYLVPGTL RPETMYGQTN
310 320 330 340 350
CWILPTGKYG AFEMANGDIF VCTERSARNM SYQQMTTGKG EYKCLAKFEG
360 370 380 390 400
SDILGAALKA PLAINPIVYV LPMLTIDENK GTGVVTSVPS DSPDDYASLQ
410 420 430 440 450
DLKAKAPLRA KFSIKDEWVL PFEVVPIIDI PGYSNESAVR AYKDLGIKSQ
460 470 480 490 500
NDRALLDQAK DLCYQKGFND GVMSVGPYAG RKVSEVKKII KDEMVASGQA
510 520 530 540 550
VDYSEPTSKV VSRSGDECVV ALSDQWYINY GDDDIEWKNQ TIKQLESMEF
560 570 580 590 600
YSAETKKKFE IALGWMNQWA CSRSFGLGTH LPWDEKFLIE SLSDSTIYMA
610 620 630 640 650
FYTVAHLLQG DVNGSKPGSA GITPKQMTSA CWDYVLMGKP YPEGCEVSEE
660 670 680 690 700
KLKELKKEFT YWYPVDIRVS GADLIQNHLT FFLYTHAAIF EKKFQPKSIR
710 720 730 740 750
ANGFVNLNGE KMSKSTGNFL TLVDSVAKFS ADGTRVALAD AGDSIEDANF
760 770 780 790 800
VDQTAVTSLL KLHTQIQWVQ ETLDSIDKFR SGPLDRIQDT IFESEINNII
810 820 830 840 850
VESEKAYQKT NFRDALHLVF FDLQNARDHY KVTTLDQMHK DLVLRFIEVQ
860 870 880 890 900
ALLIYPIAPH FAQKLFNMLG KGNILSAGWP KAGAIDYEAL KKNNYVQQTI
910 920 930 940 950
YNFRMKLQVY QKAKLKGKPA GTKAIPDQST IIVSKSYPKW QQDVLEYLAT
960 970 980 990 1000
IYNEDSKSFT KDNNAIAEEL LSREEMKPHK KNLMGFVASA IQNVKESGKD
1010 1020 1030 1040 1050
ALQTSLSFDE TSTLTDNIDY ICKTLELTSF DVKDFNEIPQ ASQSKIQPIP

GKPQFQFV
Length:1,058
Mass (Da):119,529
Last modified:May 24, 2005 - v1
Checksum:i62D8D2139322198E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64563.1.
RefSeqiXP_638060.1. XM_632968.1.

Genome annotation databases

EnsemblProtistsiDDB0231253; DDB0231253; DDB_G0285451.
GeneIDi8625106.
KEGGiddi:DDB_G0285451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000079 Genomic DNA. Translation: EAL64563.1.
RefSeqiXP_638060.1. XM_632968.1.

3D structure databases

ProteinModelPortaliQ54N83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231253.

Proteomic databases

PRIDEiQ54N83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231253; DDB0231253; DDB_G0285451.
GeneIDi8625106.
KEGGiddi:DDB_G0285451.

Organism-specific databases

dictyBaseiDDB_G0285451. leuS.

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ54N83.
KOiK01869.
OMAiSEHFPKT.
PhylomeDBiQ54N83.

Miscellaneous databases

PROiQ54N83.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiSYLC_DICDI
AccessioniPrimary (citable) accession number: Q54N83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: February 4, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.