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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

uch2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83NucleophileBy similarity1
Active sitei157Proton donorBy similarity1
Sitei172Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DDI-5689603 UCH proteinases

Protein family/group databases

MEROPSiC12.005

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
Short name:
UCH-L5
Alternative name(s):
Ubiquitin thioesterase L5
Gene namesi
Name:uch2
Synonyms:uchl5
ORF Names:DDB_G0285527
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285527 uch2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003311271 – 343Ubiquitin carboxyl-terminal hydrolase isozyme L5Add BLAST343

Proteomic databases

PaxDbiQ54N38

Interactioni

Subunit structurei

Component of the 19S (PA700) regulatory complex of the 26S proteasome.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0233072

Structurei

3D structure databases

ProteinModelPortaliQ54N38
SMRiQ54N38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiKOG2778 Eukaryota
ENOG410XP0P LUCA
InParanoidiQ54N38
KOiK05610
OMAiTIQNACA
PhylomeDBiQ54N38

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR017390 Ubiquitinyl_hydrolase_UCH37
PANTHERiPTHR10589 PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PIRSFiPIRSF038120 Ubiquitinyl_hydrolase_UCH37, 1 hit
PRINTSiPR00707 UBCTHYDRLASE

Sequencei

Sequence statusi: Complete.

Q54N38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENEGWCTI ESDPGVFTEL ITKIGVKDIQ VEELYTLDSS EYDRLKPVLG
60 70 80 90 100
LIFLFKWEKE EENRTISDNE NIFFANQVIQ NACATQAILS VLLNSEGIEL
110 120 130 140 150
GEELSNFKSF VGDFPPMMKG EAIGNSELIK ETHNSFTVQD PFIFSKKKNR
160 170 180 190 200
KPSDAFHFIS FIPFQGKVYE LDGLKKGPYC LGDCTPDNWL EIATPFIQKR
210 220 230 240 250
MEKYSQGEIR FNLMAVIKNR QTTLQEKILT LEKKKNDLEI KLSELNSGSG
260 270 280 290 300
GDNKEESGGA TPTTKEDLNF MINVVNNDIE EANNEILMEQ EKFRNWKDEN
310 320 330 340
IRRKHNFTPL ILNLIKGLAE KDNLQPLIQK AKDQISQKQQ QHK
Length:343
Mass (Da):39,325
Last modified:May 24, 2005 - v1
Checksum:iCAE23E5DB3EBE169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000079 Genomic DNA Translation: EAL64601.1
RefSeqiXP_638105.1, XM_633013.1

Genome annotation databases

EnsemblProtistsiEAL64601; EAL64601; DDB_G0285527
GeneIDi8625153
KEGGiddi:DDB_G0285527

Similar proteinsi

Entry informationi

Entry nameiUCHL5_DICDI
AccessioniPrimary (citable) accession number: Q54N38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health