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Q54N38

- UCHL5_DICDI

UniProt

Q54N38 - UCHL5_DICDI

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

uch2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831NucleophileBy similarity
    Active sitei157 – 1571Proton donorBy similarity
    Sitei172 – 1721Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro
    2. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
    Short name:
    UCH-L5
    Alternative name(s):
    Ubiquitin thioesterase L5
    Gene namesi
    Name:uch2
    Synonyms:uchl5
    ORF Names:DDB_G0285527
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0285527. uch2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Ubiquitin carboxyl-terminal hydrolase isozyme L5PRO_0000331127Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the 19S (PA700) regulatory complex of the 26S proteasome.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0233072.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54N38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG321645.
    KOiK05610.
    OMAiDEDAFHF.
    PhylomeDBiQ54N38.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    Q54N38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSENEGWCTI ESDPGVFTEL ITKIGVKDIQ VEELYTLDSS EYDRLKPVLG    50
    LIFLFKWEKE EENRTISDNE NIFFANQVIQ NACATQAILS VLLNSEGIEL 100
    GEELSNFKSF VGDFPPMMKG EAIGNSELIK ETHNSFTVQD PFIFSKKKNR 150
    KPSDAFHFIS FIPFQGKVYE LDGLKKGPYC LGDCTPDNWL EIATPFIQKR 200
    MEKYSQGEIR FNLMAVIKNR QTTLQEKILT LEKKKNDLEI KLSELNSGSG 250
    GDNKEESGGA TPTTKEDLNF MINVVNNDIE EANNEILMEQ EKFRNWKDEN 300
    IRRKHNFTPL ILNLIKGLAE KDNLQPLIQK AKDQISQKQQ QHK 343
    Length:343
    Mass (Da):39,325
    Last modified:May 24, 2005 - v1
    Checksum:iCAE23E5DB3EBE169
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64601.1.
    RefSeqiXP_638105.1. XM_633013.1.

    Genome annotation databases

    EnsemblProtistsiDDB0233072; DDB0233072; DDB_G0285527.
    GeneIDi8625153.
    KEGGiddi:DDB_G0285527.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000079 Genomic DNA. Translation: EAL64601.1 .
    RefSeqi XP_638105.1. XM_633013.1.

    3D structure databases

    ProteinModelPortali Q54N38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0233072.

    Protein family/group databases

    MEROPSi C12.005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0233072 ; DDB0233072 ; DDB_G0285527 .
    GeneIDi 8625153.
    KEGGi ddi:DDB_G0285527.

    Organism-specific databases

    dictyBasei DDB_G0285527. uch2.

    Phylogenomic databases

    eggNOGi NOG321645.
    KOi K05610.
    OMAi DEDAFHF.
    PhylomeDBi Q54N38.

    Miscellaneous databases

    PROi Q54N38.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiUCHL5_DICDI
    AccessioniPrimary (citable) accession number: Q54N38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3