Reviewed, UniProtKB/Swiss-Prot Q54MZ8 (SYMM_DICDI)

Names and origin

Protein names

Recommended name:
    Probable methionyl-tRNA synthetase, mitochondrial
    EC=6.1.1.10
Alternative name(s):
    Mitochondrial methionine--tRNA ligase
      Short name=MtMetRS

Gene names

Name:	mmetS
ORF Names:	DDB_G0285759

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 575		Probable methionyl-tRNA synthetase, mitochondrial		PRO_0000328568
Regions
	Motif	52 – 62	11	"HIGH" region By similarity
	Motif	352 – 356	5	"KMSKS" region By similarity
Sites
	Binding site	355	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54MZ8-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 2F300850EB774150	FASTA	575	65,877
	10 20 30 40 50 60 MLKLLKQIST TSTFKKPSSI NNGFININLF KNYCTSVKQE DKKKVLITTP IFYVNGPPHI 70 80 90 100 110 120 GHLYSALLGD ALGRWNRFIG NDTLFMTGTD EHGSKVDEAA KKNGLKTIDY CDKISNRFRE 130 140 150 160 170 180 LFDKADIKYD DFIRTTEPRH KEAVTAIWNR LLERGYIYKG VYKGWYCTSD ESFLTDDQVT 190 200 210 220 230 240 EGMSPITPQN PISKKCMISL ESGHEVNWIE EENYMFKLSE FSKTIENWFE EVKPIFPAIH 250 260 270 280 290 300 VNLLRYMLSQ GIKDLSISRP SSRIPWGIEV PNDPSQTIYV WLDALTNYLT VTGYPNVSPN 310 320 330 340 350 360 SSQSHWSNAT HIIGKDIIKF HSVYWPSFLI AADYPLPKSI ICHAHWTVNR EKMSKSRGNV 370 380 390 400 410 420 VDPFLAIDNH GLELIRYFLL KGGGLENDGD WSEHELAVRF KSDLADTYGN LISRCTGKAL 430 440 450 460 470 480 NPSGEWPKSV TDTSLFTMDD QKLIENSSIL VKSVSTHYDR GDFKSGIFEI MTFLYECNLY 490 500 510 520 530 540 VQNQAPWKLV PKPNRVGSDL IRLNTIIYIA IEMIRITSLL LSPIIPTSSN LTLNYLSIPL 550 560 570 ENRSNPSNFK FGYNYHQNQN NLKLPKEILI LFHKK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000079 Genomic DNA. Translation: EAL64717.1.
RefSeq	XP_638145.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54MZ8.
Genome annotation databases
GeneID	3389685.
GenomeReviews	Gene locus mmetS in contig CM000153_GR.
KEGG	ddi:DDB_0231296.
Organism-specific databases
dictyBase	DDB_G0285759. mmetS.
Phylogenomic databases
OMA	TRFIRTT.
Enzyme and pathway databases
BRENDA	6.1.1.10. 424.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMM_DICDI

Accession

Primary (citable) accession number: Q54MZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)