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Q54MJ7 (ALAM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alanine aminotransferase, mitochondrial

Short name=ALT
EC=2.6.1.2
Alternative name(s):
Glutamate pyruvate transaminase
Short name=GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene names
Name:gpt
ORF Names:DDB_G0285899
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandPyridoxal phosphate
   Molecular functionAminotransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-alanine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-alanine:2-oxoglutarate aminotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1818Mitochondrion Potential
Chain19 – 534516Probable alanine aminotransferase, mitochondrial
PRO_0000328384

Regions

Compositional bias31 – 355Poly-Thr
Compositional bias38 – 414Poly-Asn

Amino acid modifications

Modified residue3521N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54MJ7 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 3C75A9FA6ACDA4C0

FASTA53459,495
        10         20         30         40         50         60 
MFKRSLKVLL SNPPINRVKP SSTIIQPLSN TTTTTIINNN NITNFEKMTH KKSMTIDNIC 

        70         80         90        100        110        120 
QNVRNAQYAV RGELVIRAEA ISHQLQKQKT EGTKTLPFEE IVYCNIGNPQ QLKQKPLTYF 

       130        140        150        160        170        180 
RQVVSLVECP DLLDNPYVEK IYPADVISRA KEILGSINNT TGAYSNSQGI GLVLRSVADF 

       190        200        210        220        230        240 
IERRDGHKSD PSEIFLTDGA SVGVQRILKL LIKDRSDGIL IPIPQYPLYS ATIELYNGSQ 

       250        260        270        280        290        300 
LGYLLNEEKG WSLEISQLEH SYNDAVSKGI NPRALVIINP GNPTGQCLDR ANMEEIVKFC 

       310        320        330        340        350        360 
LEKNVVLLAD EVYQENVYVK ESKPFISFKK VVKDMGGDYA DLEMVSFHSV SKGFVGECGK 

       370        380        390        400        410        420 
RGGYMELNGV TQDVKAEIYK LASIGLCPNV IGQLVVDLMV RPPVAGEQSH DLYLKERDNI 

       430        440        450        460        470        480 
YESLKKRANL LTNALNNLEG VTCNPSEGAM YAFPQIRLPA KAVEYANSIG KAPDAYYCIQ 

       490        500        510        520        530 
LLEATGICVV PGSGFGQKDG TWHFRTTFLP SEEAIEGVCK RIADFHQSFM NKYK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000082 Genomic DNA. Translation: EAL64484.1.

3D structure databases

ProteinModelPortalQ54MJ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0232139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0232139; DDB0232139; DDB_G0285899.
KEGGddi:DDB_G0285899.

Organism-specific databases

dictyBaseDDB_G0285899.

Phylogenomic databases

eggNOGCOG0436.
KOK00814.
OMAFPANPQD.
PhylomeDBQ54MJ7.
ProtClustDBPTZ00377.

Enzyme and pathway databases

UniPathwayUPA00528; UER00586.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALAM_DICDI
AccessionPrimary (citable) accession number: Q54MJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase