Q54MJ7 (ALAM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alanine aminotransferase, mitochondrial
Short name=ALT
EC=2.6.1.2

Alternative name(s):
Glutamate pyruvate transaminase
Short name=GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase

Gene names

Name:	gpt
ORF Names:	DDB_G0285899

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-alanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-alanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 18	18	Mitochondrion Potential
	Chain	19 – 534	516	Probable alanine aminotransferase, mitochondrial		PRO_0000328384
Regions
	Compositional bias	31 – 35	5	Poly-Thr
	Compositional bias	38 – 41	4	Poly-Asn
Amino acid modifications
	Modified residue	352	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54MJ7 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 3C75A9FA6ACDA4C0	FASTA	534	59,495
	10 20 30 40 50 60 MFKRSLKVLL SNPPINRVKP SSTIIQPLSN TTTTTIINNN NITNFEKMTH KKSMTIDNIC 70 80 90 100 110 120 QNVRNAQYAV RGELVIRAEA ISHQLQKQKT EGTKTLPFEE IVYCNIGNPQ QLKQKPLTYF 130 140 150 160 170 180 RQVVSLVECP DLLDNPYVEK IYPADVISRA KEILGSINNT TGAYSNSQGI GLVLRSVADF 190 200 210 220 230 240 IERRDGHKSD PSEIFLTDGA SVGVQRILKL LIKDRSDGIL IPIPQYPLYS ATIELYNGSQ 250 260 270 280 290 300 LGYLLNEEKG WSLEISQLEH SYNDAVSKGI NPRALVIINP GNPTGQCLDR ANMEEIVKFC 310 320 330 340 350 360 LEKNVVLLAD EVYQENVYVK ESKPFISFKK VVKDMGGDYA DLEMVSFHSV SKGFVGECGK 370 380 390 400 410 420 RGGYMELNGV TQDVKAEIYK LASIGLCPNV IGQLVVDLMV RPPVAGEQSH DLYLKERDNI 430 440 450 460 470 480 YESLKKRANL LTNALNNLEG VTCNPSEGAM YAFPQIRLPA KAVEYANSIG KAPDAYYCIQ 490 500 510 520 530 LLEATGICVV PGSGFGQKDG TWHFRTTFLP SEEAIEGVCK RIADFHQSFM NKYK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000082 Genomic DNA. Translation: EAL64484.1.
RefSeq	XP_637993.1. XM_632901.1.
3D structure databases
ProteinModelPortal	Q54MJ7.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0232139.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0232139; DDB0232139; DDB_G0285899.
GeneID	8625344.
KEGG	ddi:DDB_G0285899.
Organism-specific databases
dictyBase	DDB_G0285899.
Phylogenomic databases
eggNOG	COG0436.
KO	K00814.
OMA	LKLMSVR.
ProtClustDB	PTZ00377.
Enzyme and pathway databases
UniPathway	UPA00528; UER00586.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALAM_DICDI

Accession

Primary (citable) accession number: Q54MJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)