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Protein

Probable alanine aminotransferase, mitochondrial

Gene

gpt

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathway: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable alanine aminotransferase, mitochondrial (gpt)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_313802. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alanine aminotransferase, mitochondrial (EC:2.6.1.2)
Short name:
ALT
Alternative name(s):
Glutamate pyruvate transaminase
Short name:
GPT
Glutamic--alanine transaminase
Glutamic--pyruvic transaminase
Gene namesi
Name:gpt
ORF Names:DDB_G0285899
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285899.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818MitochondrionSequence AnalysisAdd
BLAST
Chaini19 – 534516Probable alanine aminotransferase, mitochondrialPRO_0000328384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei352 – 3521N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0232139.

Structurei

3D structure databases

ProteinModelPortaliQ54MJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 355Poly-Thr
Compositional biasi38 – 414Poly-Asn

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0436.
InParanoidiQ54MJ7.
KOiK00814.
OMAiLNEEKCW.
PhylomeDBiQ54MJ7.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54MJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRSLKVLL SNPPINRVKP SSTIIQPLSN TTTTTIINNN NITNFEKMTH
60 70 80 90 100
KKSMTIDNIC QNVRNAQYAV RGELVIRAEA ISHQLQKQKT EGTKTLPFEE
110 120 130 140 150
IVYCNIGNPQ QLKQKPLTYF RQVVSLVECP DLLDNPYVEK IYPADVISRA
160 170 180 190 200
KEILGSINNT TGAYSNSQGI GLVLRSVADF IERRDGHKSD PSEIFLTDGA
210 220 230 240 250
SVGVQRILKL LIKDRSDGIL IPIPQYPLYS ATIELYNGSQ LGYLLNEEKG
260 270 280 290 300
WSLEISQLEH SYNDAVSKGI NPRALVIINP GNPTGQCLDR ANMEEIVKFC
310 320 330 340 350
LEKNVVLLAD EVYQENVYVK ESKPFISFKK VVKDMGGDYA DLEMVSFHSV
360 370 380 390 400
SKGFVGECGK RGGYMELNGV TQDVKAEIYK LASIGLCPNV IGQLVVDLMV
410 420 430 440 450
RPPVAGEQSH DLYLKERDNI YESLKKRANL LTNALNNLEG VTCNPSEGAM
460 470 480 490 500
YAFPQIRLPA KAVEYANSIG KAPDAYYCIQ LLEATGICVV PGSGFGQKDG
510 520 530
TWHFRTTFLP SEEAIEGVCK RIADFHQSFM NKYK
Length:534
Mass (Da):59,495
Last modified:May 24, 2005 - v1
Checksum:i3C75A9FA6ACDA4C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000082 Genomic DNA. Translation: EAL64484.1.
RefSeqiXP_637993.1. XM_632901.1.

Genome annotation databases

EnsemblProtistsiDDB0232139; DDB0232139; DDB_G0285899.
GeneIDi8625344.
KEGGiddi:DDB_G0285899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000082 Genomic DNA. Translation: EAL64484.1.
RefSeqiXP_637993.1. XM_632901.1.

3D structure databases

ProteinModelPortaliQ54MJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0232139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0232139; DDB0232139; DDB_G0285899.
GeneIDi8625344.
KEGGiddi:DDB_G0285899.

Organism-specific databases

dictyBaseiDDB_G0285899.

Phylogenomic databases

eggNOGiCOG0436.
InParanoidiQ54MJ7.
KOiK00814.
OMAiLNEEKCW.
PhylomeDBiQ54MJ7.

Enzyme and pathway databases

UniPathwayiUPA00528; UER00586.
ReactomeiREACT_313802. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ54MJ7.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiALAM_DICDI
AccessioniPrimary (citable) accession number: Q54MJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.