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Q54MB4

- ASNS_DICDI

UniProt

Q54MB4 - ASNS_DICDI

Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene

asns

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei101 – 1011GlutamineBy similarity
    Binding sitei239 – 2391ATP; via carbonyl oxygenBy similarity
    Binding sitei279 – 2791ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei355 – 3551Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi353 – 3542ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: dictyBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. asparagine biosynthetic process Source: dictyBase
    2. glutamine metabolic process Source: UniProtKB-KW
    3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_226700. ATF4 activates genes.
    UniPathwayiUPA00134; UER00195.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:asns
    Synonyms:asnA
    ORF Names:DDB_G0286059
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0286059. asns.

    Subcellular locationi

    GO - Cellular componenti

    1. phagocytic vesicle Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]PRO_0000329451Add
    BLAST

    Proteomic databases

    PRIDEiQ54MB4.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0230140.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54MB4.
    SMRiQ54MB4. Positions 3-532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 188187Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini217 – 466250Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    KOiK01953.
    OMAiDFINTAM.
    PhylomeDBiQ54MB4.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54MB4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGILAILNS LEEASKLRKK ALSLSSRLRH RGPDWNGIYQ SSDSILTHER    50
    LAIVGLENGA QPLLNEDETI ALTVNGEIYN HEKLREDLVA TGKHTFKTHS 100
    DCEPILHLYE DKGDDFVHML DGDFAFVVYN KKANSFLAAR DPIGVVPLYI 150
    GWHKDGSIWF SSEMKAIKDD CYKFQPFPPG HYFSSKTKEF VRYYKPNWIM 200
    GDSPSGVLKS EEQVLPAIKE AFEQAVVSRM MSDVPYGVLL SGGLDSSLVA 250
    SIVSRHAEQR VEDHEKSRAW WPRIHSFCIG LKDAPDLKAA RDVADYLQTV 300
    HHEYHFTVQE GIDALPDVIK HLETYDVTTI RASTPMYFLS RKIKAMGVKM 350
    VLSGEGSDEI FGGYLYFHNA PDANEFHVEC CRRIKALHSF DCLRANKSTA 400
    AWGVEVRVPF LDQRFLDVAM NIDPSHKVCH DDQGKKRMEK YILRKAFETK 450
    EGEKPYLPSS VLWRQKEQFS DGVGYSWIDG LKENAENEVS DEEFAKRESY 500
    FPDDTPTTKE AFLYRKMFEA IYPGKECMET VQRWIPTWGA SQDPSGRAQK 550
    VHLSTTE 557
    Length:557
    Mass (Da):63,487
    Last modified:May 24, 2005 - v1
    Checksum:i02E7F990A55CA254
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000085 Genomic DNA. Translation: EAL64408.1.
    RefSeqiXP_637920.1. XM_632828.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230140; DDB0230140; DDB_G0286059.
    GeneIDi8625431.
    KEGGiddi:DDB_G0286059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000085 Genomic DNA. Translation: EAL64408.1 .
    RefSeqi XP_637920.1. XM_632828.1.

    3D structure databases

    ProteinModelPortali Q54MB4.
    SMRi Q54MB4. Positions 3-532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230140.

    Proteomic databases

    PRIDEi Q54MB4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230140 ; DDB0230140 ; DDB_G0286059 .
    GeneIDi 8625431.
    KEGGi ddi:DDB_G0286059.

    Organism-specific databases

    dictyBasei DDB_G0286059. asns.

    Phylogenomic databases

    eggNOGi COG0367.
    KOi K01953.
    OMAi DFINTAM.
    PhylomeDBi Q54MB4.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .
    Reactomei REACT_226700. ATF4 activates genes.

    Miscellaneous databases

    PROi Q54MB4.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiASNS_DICDI
    AccessioniPrimary (citable) accession number: Q54MB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3