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Q54MB4 (ASNS_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Name:asns
Synonyms:asnA
ORF Names:DDB_G0286059
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]
PRO_0000329451

Regions

Domain2 – 188187Glutamine amidotransferase type-2
Domain217 – 466250Asparagine synthetase
Nucleotide binding353 – 3542ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1011Glutamine By similarity
Binding site2391ATP; via carbonyl oxygen By similarity
Binding site2791ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3551Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54MB4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 02E7F990A55CA254

FASTA55763,487
        10         20         30         40         50         60 
MCGILAILNS LEEASKLRKK ALSLSSRLRH RGPDWNGIYQ SSDSILTHER LAIVGLENGA 

        70         80         90        100        110        120 
QPLLNEDETI ALTVNGEIYN HEKLREDLVA TGKHTFKTHS DCEPILHLYE DKGDDFVHML 

       130        140        150        160        170        180 
DGDFAFVVYN KKANSFLAAR DPIGVVPLYI GWHKDGSIWF SSEMKAIKDD CYKFQPFPPG 

       190        200        210        220        230        240 
HYFSSKTKEF VRYYKPNWIM GDSPSGVLKS EEQVLPAIKE AFEQAVVSRM MSDVPYGVLL 

       250        260        270        280        290        300 
SGGLDSSLVA SIVSRHAEQR VEDHEKSRAW WPRIHSFCIG LKDAPDLKAA RDVADYLQTV 

       310        320        330        340        350        360 
HHEYHFTVQE GIDALPDVIK HLETYDVTTI RASTPMYFLS RKIKAMGVKM VLSGEGSDEI 

       370        380        390        400        410        420 
FGGYLYFHNA PDANEFHVEC CRRIKALHSF DCLRANKSTA AWGVEVRVPF LDQRFLDVAM 

       430        440        450        460        470        480 
NIDPSHKVCH DDQGKKRMEK YILRKAFETK EGEKPYLPSS VLWRQKEQFS DGVGYSWIDG 

       490        500        510        520        530        540 
LKENAENEVS DEEFAKRESY FPDDTPTTKE AFLYRKMFEA IYPGKECMET VQRWIPTWGA 

       550 
SQDPSGRAQK VHLSTTE 

« Hide

References

[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000085 Genomic DNA. Translation: EAL64408.1.
RefSeqXP_637920.1. XM_632828.1.

3D structure databases

ProteinModelPortalQ54MB4.
SMRQ54MB4. Positions 3-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230140.

Proteomic databases

PRIDEQ54MB4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230140; DDB0230140; DDB_G0286059.
GeneID8625431.
KEGGddi:DDB_G0286059.

Organism-specific databases

dictyBaseDDB_G0286059. asns.

Phylogenomic databases

eggNOGCOG0367.
OMADFINTAM.
PhylomeDBQ54MB4.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54MB4.

Entry information

Entry nameASNS_DICDI
AccessionPrimary (citable) accession number: Q54MB4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase