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Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene

asns

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable asparagine synthetase [glutamine-hydrolyzing] (asns)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei101GlutamineBy similarity1
Binding sitei239ATP; via carbonyl oxygenBy similarity1
Binding sitei279ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei355Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi353 – 354ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00134; UER00195

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:asns
Synonyms:asnA
ORF Names:DDB_G0286059
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286059 asns

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003294512 – 557Probable asparagine synthetase [glutamine-hydrolyzing]Add BLAST556

Proteomic databases

PaxDbiQ54MB4

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi44689.DDB0230140

Structurei

3D structure databases

ProteinModelPortaliQ54MB4
SMRiQ54MB4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 188Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST187
Domaini217 – 466Asparagine synthetaseAdd BLAST250

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
InParanoidiQ54MB4
KOiK01953
OMAiYFHAAPD
PhylomeDBiQ54MB4

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54MB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAILNS LEEASKLRKK ALSLSSRLRH RGPDWNGIYQ SSDSILTHER
60 70 80 90 100
LAIVGLENGA QPLLNEDETI ALTVNGEIYN HEKLREDLVA TGKHTFKTHS
110 120 130 140 150
DCEPILHLYE DKGDDFVHML DGDFAFVVYN KKANSFLAAR DPIGVVPLYI
160 170 180 190 200
GWHKDGSIWF SSEMKAIKDD CYKFQPFPPG HYFSSKTKEF VRYYKPNWIM
210 220 230 240 250
GDSPSGVLKS EEQVLPAIKE AFEQAVVSRM MSDVPYGVLL SGGLDSSLVA
260 270 280 290 300
SIVSRHAEQR VEDHEKSRAW WPRIHSFCIG LKDAPDLKAA RDVADYLQTV
310 320 330 340 350
HHEYHFTVQE GIDALPDVIK HLETYDVTTI RASTPMYFLS RKIKAMGVKM
360 370 380 390 400
VLSGEGSDEI FGGYLYFHNA PDANEFHVEC CRRIKALHSF DCLRANKSTA
410 420 430 440 450
AWGVEVRVPF LDQRFLDVAM NIDPSHKVCH DDQGKKRMEK YILRKAFETK
460 470 480 490 500
EGEKPYLPSS VLWRQKEQFS DGVGYSWIDG LKENAENEVS DEEFAKRESY
510 520 530 540 550
FPDDTPTTKE AFLYRKMFEA IYPGKECMET VQRWIPTWGA SQDPSGRAQK

VHLSTTE
Length:557
Mass (Da):63,487
Last modified:May 24, 2005 - v1
Checksum:i02E7F990A55CA254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000085 Genomic DNA Translation: EAL64408.1
RefSeqiXP_637920.1, XM_632828.1

Genome annotation databases

EnsemblProtistsiEAL64408; EAL64408; DDB_G0286059
GeneIDi8625431
KEGGiddi:DDB_G0286059

Similar proteinsi

Entry informationi

Entry nameiASNS_DICDI
AccessioniPrimary (citable) accession number: Q54MB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: February 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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