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Q54MB4

- ASNS_DICDI

UniProt

Q54MB4 - ASNS_DICDI

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Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene
asns, asnA, DDB_G0286059
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei101 – 1011Glutamine By similarity
Binding sitei239 – 2391ATP; via carbonyl oxygen By similarity
Binding sitei279 – 2791ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei355 – 3551Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi353 – 3542ATP By similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: dictyBase
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. asparagine biosynthetic process Source: dictyBase
  2. glutamine metabolic process Source: UniProtKB-KW
  3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_226700. ATF4 activates genes.
UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:asns
Synonyms:asnA
ORF Names:DDB_G0286059
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286059. asns.

Subcellular locationi

GO - Cellular componenti

  1. phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 557556Probable asparagine synthetase [glutamine-hydrolyzing]PRO_0000329451Add
BLAST

Proteomic databases

PRIDEiQ54MB4.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0230140.

Structurei

3D structure databases

ProteinModelPortaliQ54MB4.
SMRiQ54MB4. Positions 3-532.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 188187Glutamine amidotransferase type-2Add
BLAST
Domaini217 – 466250Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
KOiK01953.
OMAiDFINTAM.
PhylomeDBiQ54MB4.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54MB4-1 [UniParc]FASTAAdd to Basket

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MCGILAILNS LEEASKLRKK ALSLSSRLRH RGPDWNGIYQ SSDSILTHER    50
LAIVGLENGA QPLLNEDETI ALTVNGEIYN HEKLREDLVA TGKHTFKTHS 100
DCEPILHLYE DKGDDFVHML DGDFAFVVYN KKANSFLAAR DPIGVVPLYI 150
GWHKDGSIWF SSEMKAIKDD CYKFQPFPPG HYFSSKTKEF VRYYKPNWIM 200
GDSPSGVLKS EEQVLPAIKE AFEQAVVSRM MSDVPYGVLL SGGLDSSLVA 250
SIVSRHAEQR VEDHEKSRAW WPRIHSFCIG LKDAPDLKAA RDVADYLQTV 300
HHEYHFTVQE GIDALPDVIK HLETYDVTTI RASTPMYFLS RKIKAMGVKM 350
VLSGEGSDEI FGGYLYFHNA PDANEFHVEC CRRIKALHSF DCLRANKSTA 400
AWGVEVRVPF LDQRFLDVAM NIDPSHKVCH DDQGKKRMEK YILRKAFETK 450
EGEKPYLPSS VLWRQKEQFS DGVGYSWIDG LKENAENEVS DEEFAKRESY 500
FPDDTPTTKE AFLYRKMFEA IYPGKECMET VQRWIPTWGA SQDPSGRAQK 550
VHLSTTE 557
Length:557
Mass (Da):63,487
Last modified:May 24, 2005 - v1
Checksum:i02E7F990A55CA254
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000085 Genomic DNA. Translation: EAL64408.1.
RefSeqiXP_637920.1. XM_632828.1.

Genome annotation databases

EnsemblProtistsiDDB0230140; DDB0230140; DDB_G0286059.
GeneIDi8625431.
KEGGiddi:DDB_G0286059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000085 Genomic DNA. Translation: EAL64408.1 .
RefSeqi XP_637920.1. XM_632828.1.

3D structure databases

ProteinModelPortali Q54MB4.
SMRi Q54MB4. Positions 3-532.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230140.

Proteomic databases

PRIDEi Q54MB4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230140 ; DDB0230140 ; DDB_G0286059 .
GeneIDi 8625431.
KEGGi ddi:DDB_G0286059.

Organism-specific databases

dictyBasei DDB_G0286059. asns.

Phylogenomic databases

eggNOGi COG0367.
KOi K01953.
OMAi DFINTAM.
PhylomeDBi Q54MB4.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .
Reactomei REACT_226700. ATF4 activates genes.

Miscellaneous databases

PROi Q54MB4.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiASNS_DICDI
AccessioniPrimary (citable) accession number: Q54MB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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