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Protein

Probable asparagine synthetase [glutamine-hydrolyzing]

Gene

asns

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable asparagine synthetase [glutamine-hydrolyzing] (asns)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei101GlutamineBy similarity1
Binding sitei239ATP; via carbonyl oxygenBy similarity1
Binding sitei279ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei355Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi353 – 354ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:asns
Synonyms:asnA
ORF Names:DDB_G0286059
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286059. asns.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003294512 – 557Probable asparagine synthetase [glutamine-hydrolyzing]Add BLAST556

Proteomic databases

PaxDbiQ54MB4.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi44689.DDB0230140.

Structurei

3D structure databases

ProteinModelPortaliQ54MB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 188Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST187
Domaini217 – 466Asparagine synthetaseAdd BLAST250

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
InParanoidiQ54MB4.
KOiK01953.
OMAiDFINTAM.
PhylomeDBiQ54MB4.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54MB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAILNS LEEASKLRKK ALSLSSRLRH RGPDWNGIYQ SSDSILTHER
60 70 80 90 100
LAIVGLENGA QPLLNEDETI ALTVNGEIYN HEKLREDLVA TGKHTFKTHS
110 120 130 140 150
DCEPILHLYE DKGDDFVHML DGDFAFVVYN KKANSFLAAR DPIGVVPLYI
160 170 180 190 200
GWHKDGSIWF SSEMKAIKDD CYKFQPFPPG HYFSSKTKEF VRYYKPNWIM
210 220 230 240 250
GDSPSGVLKS EEQVLPAIKE AFEQAVVSRM MSDVPYGVLL SGGLDSSLVA
260 270 280 290 300
SIVSRHAEQR VEDHEKSRAW WPRIHSFCIG LKDAPDLKAA RDVADYLQTV
310 320 330 340 350
HHEYHFTVQE GIDALPDVIK HLETYDVTTI RASTPMYFLS RKIKAMGVKM
360 370 380 390 400
VLSGEGSDEI FGGYLYFHNA PDANEFHVEC CRRIKALHSF DCLRANKSTA
410 420 430 440 450
AWGVEVRVPF LDQRFLDVAM NIDPSHKVCH DDQGKKRMEK YILRKAFETK
460 470 480 490 500
EGEKPYLPSS VLWRQKEQFS DGVGYSWIDG LKENAENEVS DEEFAKRESY
510 520 530 540 550
FPDDTPTTKE AFLYRKMFEA IYPGKECMET VQRWIPTWGA SQDPSGRAQK

VHLSTTE
Length:557
Mass (Da):63,487
Last modified:May 24, 2005 - v1
Checksum:i02E7F990A55CA254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000085 Genomic DNA. Translation: EAL64408.1.
RefSeqiXP_637920.1. XM_632828.1.

Genome annotation databases

EnsemblProtistsiEAL64408; EAL64408; DDB_G0286059.
GeneIDi8625431.
KEGGiddi:DDB_G0286059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000085 Genomic DNA. Translation: EAL64408.1.
RefSeqiXP_637920.1. XM_632828.1.

3D structure databases

ProteinModelPortaliQ54MB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0230140.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

PaxDbiQ54MB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL64408; EAL64408; DDB_G0286059.
GeneIDi8625431.
KEGGiddi:DDB_G0286059.

Organism-specific databases

dictyBaseiDDB_G0286059. asns.

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
InParanoidiQ54MB4.
KOiK01953.
OMAiDFINTAM.
PhylomeDBiQ54MB4.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.
ReactomeiR-DDI-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ54MB4.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_DICDI
AccessioniPrimary (citable) accession number: Q54MB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.