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Protein

Ubiquitin-conjugating enzyme E2 J2

Gene

ube2j2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 J2 (EC:6.3.2.19)
Gene namesi
Name:ube2j2
ORF Names:DDB_G0286511
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286511.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 219219CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei220 – 24021Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini241 – 2411LumenalSequence Analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Ubiquitin-conjugating enzyme E2 J2PRO_0000329284Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ54LP7. 2 interactions.
STRINGi44689.DDB0233839.

Structurei

3D structure databases

ProteinModelPortaliQ54LP7.
SMRiQ54LP7. Positions 6-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi170 – 19526Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ54LP7.
KOiK04554.
OMAiHGQIIFP.
PhylomeDBiQ54LP7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54LP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQVCVTRL KKELLALSKN EDIKDIFIAK PSISNILEWH YVIYGPPDTP
60 70 80 90 100
YHGGYYHGQI IFPVEYPYKP PSILMTTPSG RFVCNSRICT SMSDFHPDTW
110 120 130 140 150
SPFWSNCTII LGLISFMTED ENGFGSIVKS KETRKQLASQ SMAFNLKNPV
160 170 180 190 200
FVKLFPELIS DYKKKGTFYS SSSTPSSTPP PSSTPSSSSS SSNISKRNVP
210 220 230 240
IPTTKTTPQD TNKKKEQKNN NIPYIILFIA LIIILLYLFL K
Length:241
Mass (Da):27,154
Last modified:May 24, 2005 - v1
Checksum:iF047CE7C91D620AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000087 Genomic DNA. Translation: EAL64182.1.
RefSeqiXP_637685.1. XM_632593.1.

Genome annotation databases

EnsemblProtistsiDDB0233839; DDB0233839; DDB_G0286511.
GeneIDi8625651.
KEGGiddi:DDB_G0286511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000087 Genomic DNA. Translation: EAL64182.1.
RefSeqiXP_637685.1. XM_632593.1.

3D structure databases

ProteinModelPortaliQ54LP7.
SMRiQ54LP7. Positions 6-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ54LP7. 2 interactions.
STRINGi44689.DDB0233839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0233839; DDB0233839; DDB_G0286511.
GeneIDi8625651.
KEGGiddi:DDB_G0286511.

Organism-specific databases

dictyBaseiDDB_G0286511.

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ54LP7.
KOiK04554.
OMAiHGQIIFP.
PhylomeDBiQ54LP7.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ54LP7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches."
    Reinders Y., Schulz I., Graef R., Sickmann A.
    J. Proteome Res. 5:589-598(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUB2J2_DICDI
AccessioniPrimary (citable) accession number: Q54LP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.