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Reviewed, UniProtKB/Swiss-Prot Q54LP7 (UB2J2_DICDI)

Last modified February 9, 2010. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ubiquitin-conjugating enzyme E2 J2
    EC=6.3.2.19
Gene names
Name: ube2j2
ORF Names: DDB_G0286511
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241Ubiquitin-conjugating enzyme E2 J2
PRO_0000329284

Regions

Topological domain1 – 219219Cytoplasmic Potential
Transmembrane220 – 24021Anchor for type IV membrane protein Potential
Topological domain2411Lumenal Potential
Compositional bias170 – 19526Ser-rich

Sites

Active site891Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54LP7-1 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: F047CE7C91D620AC

FASTA24127,154
        10         20         30         40         50         60 
MSTQVCVTRL KKELLALSKN EDIKDIFIAK PSISNILEWH YVIYGPPDTP YHGGYYHGQI 

        70         80         90        100        110        120 
IFPVEYPYKP PSILMTTPSG RFVCNSRICT SMSDFHPDTW SPFWSNCTII LGLISFMTED 

       130        140        150        160        170        180 
ENGFGSIVKS KETRKQLASQ SMAFNLKNPV FVKLFPELIS DYKKKGTFYS SSSTPSSTPP 

       190        200        210        220        230        240 
PSSTPSSSSS SSNISKRNVP IPTTKTTPQD TNKKKEQKNN NIPYIILFIA LIIILLYLFL 


K 

« Hide

References

[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches."
Reinders Y., Schulz I., Graef R., Sickmann A.
J. Proteome Res. 5:589-598(2006) [PubMed: 16512674] [Abstract]
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000087 Genomic DNA. Translation: EAL64182.1.
RefSeqXP_637685.1.

3D structure databases

HSSPHSSP built from PDB template 2F4W based on UniProtKB Q8N2K1.
SMRQ54LP7. Positions 6-162.
ModBaseSearch...

Genome annotation databases

GeneID8625651.
KEGGddi:DDB_0233839.

Organism-specific databases

dictyBaseDDB_G0286511. ube2j2.

Phylogenomic databases

HOGENOMHBG756483.
OMAPIPYVVA.
PhylomeDBQ54LP7.

Enzyme and pathway databases

BRENDA6.3.2.19. 424.

Family and domain databases

InterProIPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]
Gene3DG3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SMARTSM00212. UBCc. 1 hit.
[Graphical view]
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUB2J2_DICDI
AccessionPrimary (citable) accession number: Q54LP7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents