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Protein

Cysteine--tRNA ligase, cytoplasmic

Gene

cysS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651ZincBy similarity
Metal bindingi256 – 2561ZincBy similarity
Metal bindingi281 – 2811ZincBy similarity
Metal bindingi285 – 2851ZincBy similarity
Binding sitei317 – 3171ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase, cytoplasmic (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
ORF Names:DDB_G0287159
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287159. cysS.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Cysteine--tRNA ligase, cytoplasmicPRO_0000328521Add
BLAST

Proteomic databases

PaxDbiQ54KR1.
PRIDEiQ54KR1.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231318.

Structurei

3D structure databases

ProteinModelPortaliQ54KR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7711"HIGH" regionAdd
BLAST
Motifi314 – 3185"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2007. Eukaryota.
COG0215. LUCA.
InParanoidiQ54KR1.
KOiK01883.
OMAiFDILKLC.
PhylomeDBiQ54KR1.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54KR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENSSPKLE STSAAAASTK KPFPEWIKPK GKETELLINN SLTGGKVPFV
60 70 80 90 100
FNESGKGRSL TWYACGPTVY DASHMGHART YISFDIIRRI MKNYLGFNIQ
110 120 130 140 150
YVMNITDIDD KIIIRANENG ISHSDLSKKW ETAFFEDMKL LNVLPPDALT
160 170 180 190 200
RVTEYVPQIV EYVEKIISNG FAYESNGSVY FDTVAFSKAH DYGKLEPNSV
210 220 230 240 250
GNEKLAAEGE GSLTATSAVS EKRSGFDFAL WKKSKPGEPV WNSPWGEGRP
260 270 280 290 300
GWHIECSAMA SDLLGGNIDI HSGGSDLKFP HHDNELAQSE AFYGNRQWIN
310 320 330 340 350
YFVHSGHLLI DGLKMSKSLK NFITIKQALE KYTSRQMRMF FILHKYDKAM
360 370 380 390 400
NYSPESMGYA IEMEKTFVEF FHTAKQILRD SPLSLPQFWT QAEKDLNKHL
410 420 430 440 450
QNANDQVHQF ILDNFNTSDA LKTLSDLVNK TNVYIRSCAE QKTNPRLNLI
460 470 480 490 500
SAIAEYITYI FSVFGLTESS TASSMIGFGS AGKGNIEEEM TPILNALTQF
510 520 530 540 550
RSEVRASAIA KDTTSILKTC DNLRDEVLPL LGVKIDDKSA TTAMWKFEDK
560 570 580 590 600
ETLKKEIEQK KEIEKKKQAD KEEKEKKLKE KFEKSKIPPQ QLFINETDKY
610 620 630 640 650
SKFNELGMPT HDKEGVEITK SQLKKLQKEY DNQTKEHNNY LKSLSTSTSS
660
PTLTSTQSPQ
Length:660
Mass (Da):74,664
Last modified:May 24, 2005 - v1
Checksum:i446DA3E464E4E54F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000098 Genomic DNA. Translation: EAL63854.1.
RefSeqiXP_637369.1. XM_632277.1.

Genome annotation databases

EnsemblProtistsiDDB0231318; DDB0231318; DDB_G0287159.
GeneIDi8625991.
KEGGiddi:DDB_G0287159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000098 Genomic DNA. Translation: EAL63854.1.
RefSeqiXP_637369.1. XM_632277.1.

3D structure databases

ProteinModelPortaliQ54KR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231318.

Proteomic databases

PaxDbiQ54KR1.
PRIDEiQ54KR1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231318; DDB0231318; DDB_G0287159.
GeneIDi8625991.
KEGGiddi:DDB_G0287159.

Organism-specific databases

dictyBaseiDDB_G0287159. cysS.

Phylogenomic databases

eggNOGiKOG2007. Eukaryota.
COG0215. LUCA.
InParanoidiQ54KR1.
KOiK01883.
OMAiFDILKLC.
PhylomeDBiQ54KR1.

Miscellaneous databases

PROiQ54KR1.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiSYCC_DICDI
AccessioniPrimary (citable) accession number: Q54KR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.