Q54KR1 (SYCC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase, cytoplasmic
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
ORF Names:	DDB_G0287159

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Cysteine--tRNA ligase, cytoplasmic		PRO_0000328521
Regions
	Motif	67 – 77	11	"HIGH" region
	Motif	314 – 318	5	"KMSKS" region
Sites
	Metal binding	65	1	Zinc By similarity
	Metal binding	256	1	Zinc By similarity
	Metal binding	281	1	Zinc By similarity
	Metal binding	285	1	Zinc By similarity
	Binding site	317	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54KR1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 446DA3E464E4E54F	FASTA	660	74,664
	10 20 30 40 50 60 MSENSSPKLE STSAAAASTK KPFPEWIKPK GKETELLINN SLTGGKVPFV FNESGKGRSL 70 80 90 100 110 120 TWYACGPTVY DASHMGHART YISFDIIRRI MKNYLGFNIQ YVMNITDIDD KIIIRANENG 130 140 150 160 170 180 ISHSDLSKKW ETAFFEDMKL LNVLPPDALT RVTEYVPQIV EYVEKIISNG FAYESNGSVY 190 200 210 220 230 240 FDTVAFSKAH DYGKLEPNSV GNEKLAAEGE GSLTATSAVS EKRSGFDFAL WKKSKPGEPV 250 260 270 280 290 300 WNSPWGEGRP GWHIECSAMA SDLLGGNIDI HSGGSDLKFP HHDNELAQSE AFYGNRQWIN 310 320 330 340 350 360 YFVHSGHLLI DGLKMSKSLK NFITIKQALE KYTSRQMRMF FILHKYDKAM NYSPESMGYA 370 380 390 400 410 420 IEMEKTFVEF FHTAKQILRD SPLSLPQFWT QAEKDLNKHL QNANDQVHQF ILDNFNTSDA 430 440 450 460 470 480 LKTLSDLVNK TNVYIRSCAE QKTNPRLNLI SAIAEYITYI FSVFGLTESS TASSMIGFGS 490 500 510 520 530 540 AGKGNIEEEM TPILNALTQF RSEVRASAIA KDTTSILKTC DNLRDEVLPL LGVKIDDKSA 550 560 570 580 590 600 TTAMWKFEDK ETLKKEIEQK KEIEKKKQAD KEEKEKKLKE KFEKSKIPPQ QLFINETDKY 610 620 630 640 650 660 SKFNELGMPT HDKEGVEITK SQLKKLQKEY DNQTKEHNNY LKSLSTSTSS PTLTSTQSPQ

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000098 Genomic DNA. Translation: EAL63854.1.
RefSeq	XP_637369.1. XM_632277.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ProteinModelPortal	Q54KR1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54KR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231318; DDB0231318; DDB_G0287159.
GeneID	8625991.
GenomeReviews	Gene locus cysS in contig CM000153_GR.
KEGG	ddi:DDB_G0287159.
Organism-specific databases
dictyBase	DDB_G0287159. cysS.
Phylogenomic databases
eggNOG	KOG2007.
GeneTree	EPrGT00050000005734.
HOGENOM	HBG327651.
OMA	EALCDNV.
PhylomeDB	Q54KR1.
ProtClustDB	PTZ00399.
Family and domain databases
InterPro	IPR015803. Cys-tRNA-synt. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_DICDI

Accession

Primary (citable) accession number: Q54KR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)