Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-mannosidase F

Gene

manF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35ZincBy similarity1
Metal bindingi37ZincBy similarity1
Active sitei151NucleophileBy similarity1
Metal bindingi151ZincBy similarity1
Metal bindingi392ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578 Reactions specific to the complex N-glycan synthesis pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase F (EC:3.2.1.24)
Gene namesi
Name:manF
ORF Names:DDB_G0287231
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287231 manF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000032784521 – 994Alpha-mannosidase FAdd BLAST974

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi247N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi381N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi554N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi712N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi932N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54KN4

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231616

Structurei

3D structure databases

ProteinModelPortaliQ54KN4
SMRiQ54KN4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi663 – 667Poly-Tyr5
Compositional biasi671 – 678Poly-Gln8
Compositional biasi840 – 844Poly-Gly5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959 Eukaryota
ENOG410XQMZ LUCA
InParanoidiQ54KN4
OMAiLPYTHYD
PhylomeDBiQ54KN4

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 3 hits
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG
60 70 80 90 100
QVKSIISSMV QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV
110 120 130 140 150
KNDRIEFVNG GWVMNDEACA SLESVIRQLS NGHKFIREKF GKQPESGWQI
160 170 180 190 200
DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL KKKFKEEKNL QFKWRGSPEG
210 220 230 240 250
VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME MIELARNRSV
260 270 280 290 300
FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS
310 320 330 340 350
TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG
360 370 380 390 400
YERHVSSKLR SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK
410 420 430 440 450
KHVVQDYFSR LQKADILVSE QSEKLLASAL SQHSPTKLDI IDIGGSLNFP
460 470 480 490 500
KNNDAISFIL FNQLSWSKEE LISIKVQSVG DHGESLNSPT NNACPYVLAQ
510 520 530 540 550
EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF SSKLYYLKRK
560 570 580 590 600
SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ
610 620 630 640 650
ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI
660 670 680 690 700
LQDTHQISNR NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK
710 720 730 740 750
KINFNFHIRG INGTTTINRF TTDIDNNREL YSDNGLEMMH RKSISSQSVE
760 770 780 790 800
VGRETQSYYP TINSVYIESQ STGKRFVCNN DRSRGVSSQG QGCLEMALHR
810 820 830 840 850
SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG GGGGDDIRKQ
860 870 880 890 900
SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI
910 920 930 940 950
HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS
960 970 980 990
HRSIVGKNLF PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH
Length:994
Mass (Da):114,357
Last modified:May 24, 2005 - v1
Checksum:iCB2E2F98BFF05CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA Translation: EAL63817.1
RefSeqiXP_637323.1, XM_632231.1

Genome annotation databases

EnsemblProtistsiEAL63817; EAL63817; DDB_G0287231
GeneIDi8626020
KEGGiddi:DDB_G0287231

Entry informationi

Entry nameiMANF_DICDI
AccessioniPrimary (citable) accession number: Q54KN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health