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Q54KN4

- MANF_DICDI

UniProt

Q54KN4 - MANF_DICDI

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Protein

Alpha-mannosidase F

Gene
manF, DDB_G0287231
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Zinc By similarity
Metal bindingi37 – 371Zinc By similarity
Active sitei151 – 1511Nucleophile By similarity
Metal bindingi151 – 1511Zinc By similarity
Metal bindingi392 – 3921Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase F (EC:3.2.1.24)
Gene namesi
Name:manF
ORF Names:DDB_G0287231
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287231. manF.

Subcellular locationi

Secreted Reviewed prediction

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 994974Alpha-mannosidase FPRO_0000327845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi247 – 2471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi381 – 3811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi554 – 5541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi712 – 7121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi932 – 9321N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231616.

Structurei

3D structure databases

ProteinModelPortaliQ54KN4.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi663 – 6675Poly-Tyr
Compositional biasi671 – 6788Poly-Gln
Compositional biasi840 – 8445Poly-Gly

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0383.
OMAiVMNDEAC.
PhylomeDBiQ54KN4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KN4-1 [UniParc]FASTAAdd to Basket

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MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG    50
QVKSIISSMV QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV 100
KNDRIEFVNG GWVMNDEACA SLESVIRQLS NGHKFIREKF GKQPESGWQI 150
DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL KKKFKEEKNL QFKWRGSPEG 200
VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME MIELARNRSV 250
FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS 300
TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG 350
YERHVSSKLR SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK 400
KHVVQDYFSR LQKADILVSE QSEKLLASAL SQHSPTKLDI IDIGGSLNFP 450
KNNDAISFIL FNQLSWSKEE LISIKVQSVG DHGESLNSPT NNACPYVLAQ 500
EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF SSKLYYLKRK 550
SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ 600
ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI 650
LQDTHQISNR NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK 700
KINFNFHIRG INGTTTINRF TTDIDNNREL YSDNGLEMMH RKSISSQSVE 750
VGRETQSYYP TINSVYIESQ STGKRFVCNN DRSRGVSSQG QGCLEMALHR 800
SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG GGGGDDIRKQ 850
SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI 900
HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS 950
HRSIVGKNLF PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH 994
Length:994
Mass (Da):114,357
Last modified:May 24, 2005 - v1
Checksum:iCB2E2F98BFF05CCB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqiXP_637323.1. XM_632231.1.

Genome annotation databases

EnsemblProtistsiDDB0231616; DDB0231616; DDB_G0287231.
GeneIDi8626020.
KEGGiddi:DDB_G0287231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1 .
RefSeqi XP_637323.1. XM_632231.1.

3D structure databases

ProteinModelPortali Q54KN4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231616.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231616 ; DDB0231616 ; DDB_G0287231 .
GeneIDi 8626020.
KEGGi ddi:DDB_G0287231.

Organism-specific databases

dictyBasei DDB_G0287231. manF.

Phylogenomic databases

eggNOGi COG0383.
OMAi VMNDEAC.
PhylomeDBi Q54KN4.

Miscellaneous databases

PROi Q54KN4.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANF_DICDI
AccessioniPrimary (citable) accession number: Q54KN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3