Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-mannosidase F

Gene

manF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35ZincBy similarity1
Metal bindingi37ZincBy similarity1
Active sitei151NucleophileBy similarity1
Metal bindingi151ZincBy similarity1
Metal bindingi392ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase F (EC:3.2.1.24)
Gene namesi
Name:manF
ORF Names:DDB_G0287231
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287231. manF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000032784521 – 994Alpha-mannosidase FAdd BLAST974

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Glycosylationi381N-linked (GlcNAc...)Sequence analysis1
Glycosylationi554N-linked (GlcNAc...)Sequence analysis1
Glycosylationi712N-linked (GlcNAc...)Sequence analysis1
Glycosylationi932N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54KN4.

Structurei

3D structure databases

ProteinModelPortaliQ54KN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi663 – 667Poly-Tyr5
Compositional biasi671 – 678Poly-Gln8
Compositional biasi840 – 844Poly-Gly5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54KN4.
OMAiVMNDEAC.
PhylomeDBiQ54KN4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG
60 70 80 90 100
QVKSIISSMV QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV
110 120 130 140 150
KNDRIEFVNG GWVMNDEACA SLESVIRQLS NGHKFIREKF GKQPESGWQI
160 170 180 190 200
DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL KKKFKEEKNL QFKWRGSPEG
210 220 230 240 250
VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME MIELARNRSV
260 270 280 290 300
FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS
310 320 330 340 350
TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG
360 370 380 390 400
YERHVSSKLR SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK
410 420 430 440 450
KHVVQDYFSR LQKADILVSE QSEKLLASAL SQHSPTKLDI IDIGGSLNFP
460 470 480 490 500
KNNDAISFIL FNQLSWSKEE LISIKVQSVG DHGESLNSPT NNACPYVLAQ
510 520 530 540 550
EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF SSKLYYLKRK
560 570 580 590 600
SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ
610 620 630 640 650
ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI
660 670 680 690 700
LQDTHQISNR NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK
710 720 730 740 750
KINFNFHIRG INGTTTINRF TTDIDNNREL YSDNGLEMMH RKSISSQSVE
760 770 780 790 800
VGRETQSYYP TINSVYIESQ STGKRFVCNN DRSRGVSSQG QGCLEMALHR
810 820 830 840 850
SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG GGGGDDIRKQ
860 870 880 890 900
SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI
910 920 930 940 950
HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS
960 970 980 990
HRSIVGKNLF PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH
Length:994
Mass (Da):114,357
Last modified:May 24, 2005 - v1
Checksum:iCB2E2F98BFF05CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqiXP_637323.1. XM_632231.1.

Genome annotation databases

EnsemblProtistsiEAL63817; EAL63817; DDB_G0287231.
GeneIDi8626020.
KEGGiddi:DDB_G0287231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqiXP_637323.1. XM_632231.1.

3D structure databases

ProteinModelPortaliQ54KN4.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ54KN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL63817; EAL63817; DDB_G0287231.
GeneIDi8626020.
KEGGiddi:DDB_G0287231.

Organism-specific databases

dictyBaseiDDB_G0287231. manF.

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54KN4.
OMAiVMNDEAC.
PhylomeDBiQ54KN4.

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiQ54KN4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMANF_DICDI
AccessioniPrimary (citable) accession number: Q54KN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.