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Q54KN4

- MANF_DICDI

UniProt

Q54KN4 - MANF_DICDI

Protein

Alpha-mannosidase F

Gene

manF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi35 – 351ZincBy similarity
    Metal bindingi37 – 371ZincBy similarity
    Active sitei151 – 1511NucleophileBy similarity
    Metal bindingi151 – 1511ZincBy similarity
    Metal bindingi392 – 3921ZincBy similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase F (EC:3.2.1.24)
    Gene namesi
    Name:manF
    ORF Names:DDB_G0287231
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287231. manF.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 994974Alpha-mannosidase FPRO_0000327845Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi712 – 7121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi932 – 9321N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231616.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54KN4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi663 – 6675Poly-Tyr
    Compositional biasi671 – 6788Poly-Gln
    Compositional biasi840 – 8445Poly-Gly

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0383.
    OMAiVMNDEAC.
    PhylomeDBiQ54KN4.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 3 hits.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54KN4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG    50
    QVKSIISSMV QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV 100
    KNDRIEFVNG GWVMNDEACA SLESVIRQLS NGHKFIREKF GKQPESGWQI 150
    DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL KKKFKEEKNL QFKWRGSPEG 200
    VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME MIELARNRSV 250
    FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS 300
    TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG 350
    YERHVSSKLR SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK 400
    KHVVQDYFSR LQKADILVSE QSEKLLASAL SQHSPTKLDI IDIGGSLNFP 450
    KNNDAISFIL FNQLSWSKEE LISIKVQSVG DHGESLNSPT NNACPYVLAQ 500
    EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF SSKLYYLKRK 550
    SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ 600
    ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI 650
    LQDTHQISNR NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK 700
    KINFNFHIRG INGTTTINRF TTDIDNNREL YSDNGLEMMH RKSISSQSVE 750
    VGRETQSYYP TINSVYIESQ STGKRFVCNN DRSRGVSSQG QGCLEMALHR 800
    SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG GGGGDDIRKQ 850
    SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI 900
    HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS 950
    HRSIVGKNLF PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH 994
    Length:994
    Mass (Da):114,357
    Last modified:May 24, 2005 - v1
    Checksum:iCB2E2F98BFF05CCB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000099 Genomic DNA. Translation: EAL63817.1.
    RefSeqiXP_637323.1. XM_632231.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231616; DDB0231616; DDB_G0287231.
    GeneIDi8626020.
    KEGGiddi:DDB_G0287231.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000099 Genomic DNA. Translation: EAL63817.1 .
    RefSeqi XP_637323.1. XM_632231.1.

    3D structure databases

    ProteinModelPortali Q54KN4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231616.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231616 ; DDB0231616 ; DDB_G0287231 .
    GeneIDi 8626020.
    KEGGi ddi:DDB_G0287231.

    Organism-specific databases

    dictyBasei DDB_G0287231. manF.

    Phylogenomic databases

    eggNOGi COG0383.
    OMAi VMNDEAC.
    PhylomeDBi Q54KN4.

    Miscellaneous databases

    PROi Q54KN4.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 3 hits.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMANF_DICDI
    AccessioniPrimary (citable) accession number: Q54KN4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3