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Q54KN4 (MANF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-mannosidase F

EC=3.2.1.24
Gene names
Name:manF
ORF Names:DDB_G0287231
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length994 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 994974Alpha-mannosidase F
PRO_0000327845

Regions

Compositional bias663 – 6675Poly-Tyr
Compositional bias671 – 6788Poly-Gln
Compositional bias840 – 8445Poly-Gly

Sites

Active site1511Nucleophile By similarity
Metal binding351Zinc By similarity
Metal binding371Zinc By similarity
Metal binding1511Zinc By similarity
Metal binding3921Zinc By similarity

Amino acid modifications

Glycosylation2471N-linked (GlcNAc...) Potential
Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential
Glycosylation7121N-linked (GlcNAc...) Potential
Glycosylation9321N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q54KN4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: CB2E2F98BFF05CCB

FASTA994114,357
        10         20         30         40         50         60 
MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG QVKSIISSMV 

        70         80         90        100        110        120 
QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV KNDRIEFVNG GWVMNDEACA 

       130        140        150        160        170        180 
SLESVIRQLS NGHKFIREKF GKQPESGWQI DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL 

       190        200        210        220        230        240 
KKKFKEEKNL QFKWRGSPEG VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME 

       250        260        270        280        290        300 
MIELARNRSV FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS 

       310        320        330        340        350        360 
TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG YERHVSSKLR 

       370        380        390        400        410        420 
SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK KHVVQDYFSR LQKADILVSE 

       430        440        450        460        470        480 
QSEKLLASAL SQHSPTKLDI IDIGGSLNFP KNNDAISFIL FNQLSWSKEE LISIKVQSVG 

       490        500        510        520        530        540 
DHGESLNSPT NNACPYVLAQ EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF 

       550        560        570        580        590        600 
SSKLYYLKRK SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ 

       610        620        630        640        650        660 
ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI LQDTHQISNR 

       670        680        690        700        710        720 
NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK KINFNFHIRG INGTTTINRF 

       730        740        750        760        770        780 
TTDIDNNREL YSDNGLEMMH RKSISSQSVE VGRETQSYYP TINSVYIESQ STGKRFVCNN 

       790        800        810        820        830        840 
DRSRGVSSQG QGCLEMALHR SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG 

       850        860        870        880        890        900 
GGGGDDIRKQ SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI 

       910        920        930        940        950        960 
HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS HRSIVGKNLF 

       970        980        990 
PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqXP_637323.1. XM_632231.1.

3D structure databases

ProteinModelPortalQ54KN4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231616.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231616; DDB0231616; DDB_G0287231.
GeneID8626020.
KEGGddi:DDB_G0287231.

Organism-specific databases

dictyBaseDDB_G0287231. manF.

Phylogenomic databases

eggNOGCOG0383.
OMAVMNDEAC.
PhylomeDBQ54KN4.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

PROQ54KN4.

Entry information

Entry nameMANF_DICDI
AccessionPrimary (citable) accession number: Q54KN4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase