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Protein

Alpha-mannosidase F

Gene

manF

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351ZincBy similarity
Metal bindingi37 – 371ZincBy similarity
Active sitei151 – 1511NucleophileBy similarity
Metal bindingi151 – 1511ZincBy similarity
Metal bindingi392 – 3921ZincBy similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase F (EC:3.2.1.24)
Gene namesi
Name:manF
ORF Names:DDB_G0287231
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287231. manF.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 994974Alpha-mannosidase FPRO_0000327845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi712 – 7121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi932 – 9321N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231616.

Structurei

3D structure databases

ProteinModelPortaliQ54KN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi663 – 6675Poly-Tyr
Compositional biasi671 – 6788Poly-Gln
Compositional biasi840 – 8445Poly-Gly

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ54KN4.
OMAiVMNDEAC.
PhylomeDBiQ54KN4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNFYYFILI LLFFNEVCYS LKDGEIKLHL IPHSHCDSGW TSTMNEYYMG
60 70 80 90 100
QVKSIISSMV QSLNVESNPP RKFVWSEIGF LEQWWDDMPI EIKNDFIKHV
110 120 130 140 150
KNDRIEFVNG GWVMNDEACA SLESVIRQLS NGHKFIREKF GKQPESGWQI
160 170 180 190 200
DPFGHSSLTP TLQAQFGYKH VVLNRIHYEL KKKFKEEKNL QFKWRGSPEG
210 220 230 240 250
VGPKSDILAH VFDDFYTSPP HMSFDGYNFL AYGLPRLTME MIELARNRSV
260 270 280 290 300
FYKSPHVLIP MGGDFAFKNA YKSFEQMDQL VASINGQHAN GESNVICQYS
310 320 330 340 350
TLADFFSDTI NWHNENKVSF NYYDSDFFPY ADDSNTYWTG YYTSRPLLKG
360 370 380 390 400
YERHVSSKLR SAEILSALQN DEKYYPNQLL NASKQVSILQ HHDAISGTSK
410 420 430 440 450
KHVVQDYFSR LQKADILVSE QSEKLLASAL SQHSPTKLDI IDIGGSLNFP
460 470 480 490 500
KNNDAISFIL FNQLSWSKEE LISIKVQSVG DHGESLNSPT NNACPYVLAQ
510 520 530 540 550
EDFLNEIEID CSPRSDFKSD QSDDHKEFIQ IDFPAKLKPF SSKLYYLKRK
560 570 580 590 600
SNPNKSNWVL PKTNHFNSIE NSIYTANLDE NYLIKSLKSK SSRHGGGANQ
610 620 630 640 650
ITEINQQLLT YSDIGGAYIF RTNKQVFQPP RQVYSTFTYI GKFYQEAQSI
660 670 680 690 700
LQDTHQISNR NGYYYYYGNN QQQQQQQQTI STFNYNSIKL INTGNEMIDK
710 720 730 740 750
KINFNFHIRG INGTTTINRF TTDIDNNREL YSDNGLEMMH RKSISSQSVE
760 770 780 790 800
VGRETQSYYP TINSVYIESQ STGKRFVCNN DRSRGVSSQG QGCLEMALHR
810 820 830 840 850
SLTYEDGKGL EIPAIDESSI NARFECYLDE VPSNSQQSNG GGGGDDIRKQ
860 870 880 890 900
SINYQHKFQI YQGQDSSYMS SKSFMLKPLP EFIHILSMER SGPRSIKLRI
910 920 930 940 950
HNIENNNQSP ITFDLNGLFS FIKSIKSIKE YNLSLINRFV DNNIDNIISS
960 970 980 990
HRSIVGKNLF PIKDTPTRFN PINTKQTKIT LYPSEIKAIE ITYH
Length:994
Mass (Da):114,357
Last modified:May 24, 2005 - v1
Checksum:iCB2E2F98BFF05CCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqiXP_637323.1. XM_632231.1.

Genome annotation databases

EnsemblProtistsiDDB0231616; DDB0231616; DDB_G0287231.
GeneIDi8626020.
KEGGiddi:DDB_G0287231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63817.1.
RefSeqiXP_637323.1. XM_632231.1.

3D structure databases

ProteinModelPortaliQ54KN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231616.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231616; DDB0231616; DDB_G0287231.
GeneIDi8626020.
KEGGiddi:DDB_G0287231.

Organism-specific databases

dictyBaseiDDB_G0287231. manF.

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ54KN4.
OMAiVMNDEAC.
PhylomeDBiQ54KN4.

Miscellaneous databases

PROiQ54KN4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 3 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANF_DICDI
AccessioniPrimary (citable) accession number: Q54KN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.