Q54KM6 (KAT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kynurenine--oxoglutarate transaminase
EC=2.6.1.7

Alternative name(s):
Glutamine transaminase K
EC=4.4.1.13
Glutamine--phenylpyruvate transaminase
EC=2.6.1.64
Kynurenine aminotransferase

Gene names

Name:	ccbl
Synonyms:	kat
ORF Names:	DDB_G0287269

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond By similarity.
Catalytic activity	L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate. L-glutamine + phenylpyruvate = 2-oxoglutaramate + L-phenylalanine. RS-CH(2)-CH(NH3+)COO- = RSH + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Lyase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular modified amino acid metabolic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-S-conjugate beta-lyase activity Inferred from electronic annotation. Source: EC glutamine-phenylpyruvate transaminase activity Inferred from electronic annotation. Source: EC kynurenine-oxoglutarate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Kynurenine--oxoglutarate transaminase		PRO_0000327725
Sites
	Binding site	46	1	Substrate; via amide nitrogen By similarity
	Binding site	198	1	Substrate By similarity
	Binding site	413	1	Substrate By similarity
Amino acid modifications
	Modified residue	262	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54KM6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 5E86530007DCB3F1	FASTA	435	48,553
	10 20 30 40 50 60 MLRNTVKRLM TYTFKPSKQT SSFGPSVWLE FSPLAIKYNA VNLGQGFPNF EPPKFVKDAM 70 80 90 100 110 120 IKTIEVGGFN QYTRSPGHIR LVKALSSVYS PYFGRELNAM TEIMVGVGAS ESLFAAISSI 130 140 150 160 170 180 VNEGDEVILI EPFFDIYIGP ILMAGGIPKF VTLKEEESSQ AGSSDKKRSS KHWKINKEEL 190 200 210 220 230 240 AAAFTDKTKL IILNNPHNPV GKVYSKEELQ EIADVVAKHG PNTTVISDEV YEWMTFDGEE 250 260 270 280 290 300 HHRFATLPGM WERTITIGSA GKTFSITGWK VGWCIGPSNI IGAIANTHQY VPFSVPTPTQ 310 320 330 340 350 360 EAVAIALEQP NIKDYFKELA TMYQNKRDTL LNSLTQAGLD PVIPQGTYFI MGDTSSIHLQ 370 380 390 400 410 420 GDQGKDTSIT GMGLHLRDWN IARYLTTEYG VTTIPPSAFY CDDHQKIPEN FVRFTFCKDD 430 LTLQKAHDNL LKLKK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000099 Genomic DNA. Translation: EAL63836.1.
RefSeq	XP_637331.1. XM_632239.1.
3D structure databases
HSSP	HSSP built from PDB template 1YIZ based on UniProtKB Q95VY4.
ProteinModelPortal	Q54KM6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54KM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231138; DDB0231138; DDB_G0287269.
GeneID	8626029.
GenomeReviews	Gene locus ccbl in contig CM000154_GR.
KEGG	ddi:DDB_G0287269.
Organism-specific databases
dictyBase	DDB_G0287269. ccbl.
Phylogenomic databases
eggNOG	KOG0257.
GeneTree	EPrGT00050000005635.
HOGENOM	HBG645860.
OMA	RNLQMVH.
PhylomeDB	Q54KM6.
ProtClustDB	CLSZ2430022.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KAT_DICDI

Accession

Primary (citable) accession number: Q54KM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)