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Protein

Kynurenine--oxoglutarate transaminase

Gene

ccbl

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (By similarity).By similarity

Catalytic activityi

L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.
L-glutamine + phenylpyruvate = 2-oxoglutaramate + L-phenylalanine.
An L-cysteine-S-conjugate + H2O = RSH + NH3 + pyruvate.

Cofactori

Pathwayi: L-kynurenine degradation

This protein is involved in step 1 of the subpathway that synthesizes kynurenate from L-kynurenine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Kynurenine--oxoglutarate transaminase (ccbl)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes kynurenate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461Substrate; via amide nitrogenBy similarity
Binding sitei198 – 1981SubstrateBy similarity
Binding sitei413 – 4131SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Lyase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DDI-70614. Amino acid synthesis and interconversion (transamination).
R-DDI-71182. Phenylalanine and tyrosine catabolism.
R-DDI-71240. Tryptophan catabolism.
UniPathwayiUPA00334; UER00726.

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine--oxoglutarate transaminase (EC:2.6.1.7)
Alternative name(s):
Glutamine transaminase K (EC:4.4.1.13)
Glutamine--phenylpyruvate transaminase (EC:2.6.1.64)
Kynurenine aminotransferase
Gene namesi
Name:ccbl
Synonyms:kat
ORF Names:DDB_G0287269
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287269. ccbl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Kynurenine--oxoglutarate transaminasePRO_0000327725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei262 – 2621N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ54KM6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231138.

Structurei

3D structure databases

ProteinModelPortaliQ54KM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0257. Eukaryota.
COG0436. LUCA.
InParanoidiQ54KM6.
KOiK00816.
OMAiARFRHFS.
PhylomeDBiQ54KM6.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54KM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRNTVKRLM TYTFKPSKQT SSFGPSVWLE FSPLAIKYNA VNLGQGFPNF
60 70 80 90 100
EPPKFVKDAM IKTIEVGGFN QYTRSPGHIR LVKALSSVYS PYFGRELNAM
110 120 130 140 150
TEIMVGVGAS ESLFAAISSI VNEGDEVILI EPFFDIYIGP ILMAGGIPKF
160 170 180 190 200
VTLKEEESSQ AGSSDKKRSS KHWKINKEEL AAAFTDKTKL IILNNPHNPV
210 220 230 240 250
GKVYSKEELQ EIADVVAKHG PNTTVISDEV YEWMTFDGEE HHRFATLPGM
260 270 280 290 300
WERTITIGSA GKTFSITGWK VGWCIGPSNI IGAIANTHQY VPFSVPTPTQ
310 320 330 340 350
EAVAIALEQP NIKDYFKELA TMYQNKRDTL LNSLTQAGLD PVIPQGTYFI
360 370 380 390 400
MGDTSSIHLQ GDQGKDTSIT GMGLHLRDWN IARYLTTEYG VTTIPPSAFY
410 420 430
CDDHQKIPEN FVRFTFCKDD LTLQKAHDNL LKLKK
Length:435
Mass (Da):48,553
Last modified:May 24, 2005 - v1
Checksum:i5E86530007DCB3F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63836.1.
RefSeqiXP_637331.1. XM_632239.1.

Genome annotation databases

EnsemblProtistsiDDB0231138; DDB0231138; DDB_G0287269.
GeneIDi8626029.
KEGGiddi:DDB_G0287269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000099 Genomic DNA. Translation: EAL63836.1.
RefSeqiXP_637331.1. XM_632239.1.

3D structure databases

ProteinModelPortaliQ54KM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231138.

Proteomic databases

PaxDbiQ54KM6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231138; DDB0231138; DDB_G0287269.
GeneIDi8626029.
KEGGiddi:DDB_G0287269.

Organism-specific databases

dictyBaseiDDB_G0287269. ccbl.

Phylogenomic databases

eggNOGiKOG0257. Eukaryota.
COG0436. LUCA.
InParanoidiQ54KM6.
KOiK00816.
OMAiARFRHFS.
PhylomeDBiQ54KM6.

Enzyme and pathway databases

UniPathwayiUPA00334; UER00726.
ReactomeiR-DDI-70614. Amino acid synthesis and interconversion (transamination).
R-DDI-71182. Phenylalanine and tyrosine catabolism.
R-DDI-71240. Tryptophan catabolism.

Miscellaneous databases

PROiQ54KM6.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAT_DICDI
AccessioniPrimary (citable) accession number: Q54KM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: December 9, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.