Q54KF3 (ADA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	ada
Synonyms:	add
ORF Names:	DDB_G0287371

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of adenosine. Plays an important role in purine metabolism and in adenosine homeostasis, and may thereby contribute to cellular signaling events By similarity.
Catalytic activity	Adenosine + H2O = inosine + NH3.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adenine catabolic process Inferred from sequence or structural similarity. Source: dictyBase adenosine catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome hypoxanthine salvage Inferred from Biological aspect of Ancestor. Source: RefGenome inosine biosynthetic process Inferred from Biological aspect of Ancestor. Source: RefGenome purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	adenine deaminase activity Inferred from sequence or structural similarity. Source: dictyBase adenosine deaminase activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 772	772	Probable adenosine deaminase		PRO_0000350574
Sites
	Active site	210	1	Proton donor By similarity
	Metal binding	22	1	Zinc; catalytic By similarity
	Metal binding	24	1	Zinc; catalytic By similarity
	Metal binding	207	1	Zinc; catalytic By similarity
	Metal binding	288	1	Zinc; catalytic By similarity
	Binding site	24	1	Substrate By similarity
	Binding site	26	1	Substrate By similarity
	Binding site	180	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Site	231	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54KF3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 00443985D821FEA3	FASTA	772	87,516
	10 20 30 40 50 60 MIENNKEITL DIIKLLPKAE LHRHLDGSIR ISTLLELAKE QNVELPTYDQ NELAKLIHKD 70 80 90 100 110 120 ENCSGLVNFL EAFQYTCSVL QHAYAITRVF YEMCEDAVAD GVSYLEIRFS PVLHTSFGLS 130 140 150 160 170 180 LSEVMEAVCD GMAIAELNLP IKARIIVCGL RHLDPSISKD LAEITWRYRH KGAIAFDLAG 190 200 210 220 230 240 PEDGFSSKHH KEAFSIIRNK GINCTLHSGE DSNWTSVADS IHHCGAHRIG HGIAIQQNEE 250 260 270 280 290 300 LLNHVVNRRI PIECCITSNY QIKAISNASE HPIRKYFDSG AIVSICCDNC TMSNITLSGE 310 320 330 340 350 360 YKLAIDTFNF KVEEVIRLID YSFASSFIDP PLKINIRRES FKKALKIFQT NGYDISGVIE 370 380 390 400 410 420 NKFYYFEEIG LDVELEIQNN LANNFSLGKN VIRNYPQITL ELLENLPKSD LHCRFDGGVS 430 440 450 460 470 480 IEQIWNEVQL LGIDKCEKSK QEFLKKLSSK HLACYANFKD FKEFKSLIQS SSHTPQTIRL 490 500 510 520 530 540 SKEIINLLLQ TPEQINRAFD DIIKVALKDK VQYLELMIRP NSHSRNGLTK EQVLALIIEN 550 560 570 580 590 600 KDKWEKSSSI KIGLVVFSSS TSDDPIETLD SARLAIANRN SGVIGFGIFG ADPISPTESR 610 620 630 640 650 660 HFSQTFSLLK ENNFNLVQFA GKSDVESLIS TIHCSGSTRL SGAFQSHKIP RLMSYLGNYS 670 680 690 700 710 720 IPVEISLTEK LKSFTSDDLS FTTPIRHLLD GKCPVVICSF RSSLYPYSRS KMYYKIVKNA 730 740 750 760 770 KLDFKQVVRL LKNPFAYNFQ SHQQRIELVQ QFNKLSKEYL NSVNINYSNI II

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000100 Genomic DNA. Translation: EAL63753.1.
RefSeq	XP_637270.1. XM_632178.1.
3D structure databases
HSSP	HSSP built from PDB template 1A4M based on UniProtKB P03958.
ProteinModelPortal	Q54KF3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54KF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0230171; DDB0230171; DDB_G0287371.
GeneID	8626101.
GenomeReviews	Gene locus ada in contig CM000154_GR.
KEGG	ddi:DDB_G0287371.
Organism-specific databases
dictyBase	DDB_G0287371. ada.
Phylogenomic databases
eggNOG	KOG1097.
GeneTree	EPrGT00050000005197.
PhylomeDB	Q54KF3.
ProtClustDB	CLSZ2728871.
Family and domain databases
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 2 hits. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ADA_DICDI

Accession

Primary (citable) accession number: Q54KF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 24, 2005
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)