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Protein

Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial

Gene

mccA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity

Catalytic activityi

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
  • biotinBy similarity

Pathwayi: L-leucine degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (mccb), Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (mccA)
  3. Methylglutaconyl-CoA hydratase, mitochondrial (auh)
This subpathway is part of the pathway L-leucine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144ATPBy similarity1
Binding sitei228ATPBy similarity1
Binding sitei263ATPBy similarity1
Active sitei320By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-196780 Biotin transport and metabolism
R-DDI-70895 Branched-chain amino acid catabolism
UniPathwayiUPA00363; UER00861

Names & Taxonomyi

Protein namesi
Recommended name:
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (EC:6.4.1.4)
Short name:
MCCase subunit alpha
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 1
3-methylcrotonyl-CoA carboxylase biotin-containing subunit
3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
Gene namesi
Name:mccA
Synonyms:mccc1
ORF Names:DDB_G0287377
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287377 mccA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000327665? – 699Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei665N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ54KE6
PRIDEiQ54KE6

Interactioni

Subunit structurei

Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0230065

Structurei

3D structure databases

ProteinModelPortaliQ54KE6
SMRiQ54KE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 475Biotin carboxylationAdd BLAST446
Domaini148 – 345ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini624 – 699Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST76

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0238 Eukaryota
COG4770 LUCA
InParanoidiQ54KE6
KOiK01968
OMAiFVEICSH
PhylomeDBiQ54KE6

Family and domain databases

InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLGKLVKK DAFFYRYITN VNKDLKIKPI TKILIANRGE IACRVMRTAK
60 70 80 90 100
SKGVKTVAVY SEADKNSLHV SMADESYLIG PAAAKESYLC GNKIIDVAKR
110 120 130 140 150
SGAQAIHPGY GFLSENSDFA DLCEREGIIF IGPPSDAIKA MGSKSASKDI
160 170 180 190 200
MIKAGVPTIP GYHGEDQSMS VLKSEAAKIG YPVLIKAVMG GGGKGMRIVE
210 220 230 240 250
REEDLEDGVE SSKREATASF GDSRVLVEKY LVHPRHVEIQ VFADRHGNCV
260 270 280 290 300
HLFERDCSVQ RRHQKIIEEA PAPHLSEELR KKMGDAAVAA AKAVGYVGAG
310 320 330 340 350
TVEFILSADN SFFFMEMNTR LQVEHPITEM ITKQDLVEWQ LKVAESQTLP
360 370 380 390 400
MEQEQLKIHG HSFEARIYAE NPDSDFLPGT GKLAHLSTPT PSDTLRVETG
410 420 430 440 450
VRQGDEVSVY YDPMIAKLVV WDQDREKALR YLRNALDEYH IIGLNTNISF
460 470 480 490 500
LKRLSTHPSF MAGEVETGFI PIHRESLMAP QAPMSDDSLA LAATSLLLKE
510 520 530 540 550
ITQQKSKEDP NSPWSSLGGF RINHNLKKQV KFNQKDNKVV VNVEFIGGGG
560 570 580 590 600
AAANGKHNFK VTLDNGNVVE VLDAKLNQNN ETISAHVNGR FYNNIKSVIV
610 620 630 640 650
KDTLTIFNEG QQYQLDIPQD VKPKGADGVL GSLVSPMPGK ITKVMVNVGD
660 670 680 690
MVKKGQPILL MEAMKMEHTI RSPIDGKVES LPYNVNEIVE DKKTLAVIV
Length:699
Mass (Da):77,089
Last modified:May 24, 2005 - v1
Checksum:iB1FD14E7E286A96D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000100 Genomic DNA Translation: EAL63756.1
RefSeqiXP_637277.1, XM_632185.1

Genome annotation databases

EnsemblProtistsiEAL63756; EAL63756; DDB_G0287377
GeneIDi8626108
KEGGiddi:DDB_G0287377

Similar proteinsi

Entry informationi

Entry nameiMCCA_DICDI
AccessioniPrimary (citable) accession number: Q54KE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health