Q54KE6 (MCCA_DICDI) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 69.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Short name=MCCase subunit alpha EC=6.4.1.4 Alternative name(s): 3-methylcrotonyl-CoA carboxylase 1 3-methylcrotonyl-CoA carboxylase biotin-containing subunit 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha | ||||||
| Gene names |
| ||||||
| Organism | Dictyostelium discoideum (Slime mold) | ||||||
| Taxonomic identifier | 44689 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium |
Protein attributes
| Sequence length | 699 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA. |
| Cofactor | Binds 2 manganese ions per subunit By similarity. Biotin By similarity. |
| Pathway | |
| Subunit structure | Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits By similarity. |
| Subcellular location | Mitochondrion matrix By similarity. |
| Sequence similarities | Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | ATP-binding Biotin Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | leucine catabolic process Inferred from sequence or structural similarity. Source: dictyBase |
| Cellular component | mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW biotin carboxylase activityInferred from electronic annotation. Source: InterPro metal ion bindingInferred from electronic annotation. Source: InterPro methylcrotonoyl-CoA carboxylase activityInferred from sequence or structural similarity. Source: dictyBase |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion Potential | |||||||
| Chain | ? – 699 | Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial | PRO_0000327665 | ||||||
Regions | |||||||||
| Domain | 30 – 475 | 446 | Biotin carboxylation | ||||||
| Domain | 148 – 345 | 198 | ATP-grasp | ||||||
| Domain | 616 – 698 | 83 | Biotinyl-binding | ||||||
Sites | |||||||||
| Active site | 320 | 1 | By similarity | ||||||
| Binding site | 144 | 1 | ATP By similarity | ||||||
| Binding site | 228 | 1 | ATP By similarity | ||||||
| Binding site | 263 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 665 | 1 | N6-biotinyllysine By similarity | ||||||
Sequences
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References
| [1] | "The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A.Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AAFI02000100 Genomic DNA. Translation: EAL63756.1. |
| RefSeq | XP_637277.1. XM_632185.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BNC based on UniProtKB P24182. |
| ProteinModelPortal | Q54KE6. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q54KE6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblProtists | DDB0230065; DDB0230065; DDB_G0287377. |
| GeneID | 8626108. |
| GenomeReviews | Gene locus mccA in contig CM000154_GR. |
| KEGG | ddi:DDB_G0287377. |
Organism-specific databases | |
| dictyBase | DDB_G0287377. mccA. |
Phylogenomic databases | |
| eggNOG | KOG0238. |
| GeneTree | EPrGT00050000001985. |
| HOGENOM | HBG535236. |
| OMA | HLSTPRA. |
| PhylomeDB | Q54KE6. |
| ProtClustDB | CLSZ2430009. |
Family and domain databases | |
| InterPro | IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR001882. Biotin_BS. IPR011764. Biotin_carboxylation_dom. IPR005482. Biotin_COase_C. IPR000089. Biotin_lipoyl. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR005481. CarbamoylP_synth_lsu_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. IPR011053. Single_hybrid_motif. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| KO | K01968. |
| Pfam | PF02785. Biotin_carb_C. 1 hit. PF00364. Biotin_lipoyl. 1 hit. PF00289. CPSase_L_chain. 1 hit. PF02786. CPSase_L_D2. 1 hit. [Graphical view] |
| SMART | SM00878. Biotin_carb_C. 1 hit. [Graphical view] |
| SUPFAM | SSF51230. Hybrid_motif. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS50979. BC. 1 hit. PS00188. BIOTIN. 1 hit. PS50968. BIOTINYL_LIPOYL. 1 hit. PS00867. CPSASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MCCA_DICDI | ||||||||
| Accession | Primary (citable) accession number: Q54KE6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Dictyostelium discoideum Dictyostelium discoideum: entries, gene names and cross-references to dictyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with