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Protein

Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial

Gene

mccA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity

Catalytic activityi

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
  • biotinBy similarity

Pathwayi: L-leucine degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (mccA), Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (mccb)
  3. Methylglutaconyl-CoA hydratase, mitochondrial (auh)
This subpathway is part of the pathway L-leucine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144ATPBy similarity1
Binding sitei228ATPBy similarity1
Binding sitei263ATPBy similarity1
Active sitei320By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-196780. Biotin transport and metabolism.
R-DDI-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00363; UER00861.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (EC:6.4.1.4)
Short name:
MCCase subunit alpha
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 1
3-methylcrotonyl-CoA carboxylase biotin-containing subunit
3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
Gene namesi
Name:mccA
Synonyms:mccc1
ORF Names:DDB_G0287377
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287377. mccA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000327665? – 699Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei665N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ54KE6.
PRIDEiQ54KE6.

Interactioni

Subunit structurei

Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0230065.

Structurei

3D structure databases

ProteinModelPortaliQ54KE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 475Biotin carboxylationAdd BLAST446
Domaini148 – 345ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini624 – 699Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Curated
Contains 1 biotinyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0238. Eukaryota.
COG4770. LUCA.
InParanoidiQ54KE6.
KOiK01968.
OMAiYQLCARK.
PhylomeDBiQ54KE6.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54KE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLGKLVKK DAFFYRYITN VNKDLKIKPI TKILIANRGE IACRVMRTAK
60 70 80 90 100
SKGVKTVAVY SEADKNSLHV SMADESYLIG PAAAKESYLC GNKIIDVAKR
110 120 130 140 150
SGAQAIHPGY GFLSENSDFA DLCEREGIIF IGPPSDAIKA MGSKSASKDI
160 170 180 190 200
MIKAGVPTIP GYHGEDQSMS VLKSEAAKIG YPVLIKAVMG GGGKGMRIVE
210 220 230 240 250
REEDLEDGVE SSKREATASF GDSRVLVEKY LVHPRHVEIQ VFADRHGNCV
260 270 280 290 300
HLFERDCSVQ RRHQKIIEEA PAPHLSEELR KKMGDAAVAA AKAVGYVGAG
310 320 330 340 350
TVEFILSADN SFFFMEMNTR LQVEHPITEM ITKQDLVEWQ LKVAESQTLP
360 370 380 390 400
MEQEQLKIHG HSFEARIYAE NPDSDFLPGT GKLAHLSTPT PSDTLRVETG
410 420 430 440 450
VRQGDEVSVY YDPMIAKLVV WDQDREKALR YLRNALDEYH IIGLNTNISF
460 470 480 490 500
LKRLSTHPSF MAGEVETGFI PIHRESLMAP QAPMSDDSLA LAATSLLLKE
510 520 530 540 550
ITQQKSKEDP NSPWSSLGGF RINHNLKKQV KFNQKDNKVV VNVEFIGGGG
560 570 580 590 600
AAANGKHNFK VTLDNGNVVE VLDAKLNQNN ETISAHVNGR FYNNIKSVIV
610 620 630 640 650
KDTLTIFNEG QQYQLDIPQD VKPKGADGVL GSLVSPMPGK ITKVMVNVGD
660 670 680 690
MVKKGQPILL MEAMKMEHTI RSPIDGKVES LPYNVNEIVE DKKTLAVIV
Length:699
Mass (Da):77,089
Last modified:May 24, 2005 - v1
Checksum:iB1FD14E7E286A96D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000100 Genomic DNA. Translation: EAL63756.1.
RefSeqiXP_637277.1. XM_632185.1.

Genome annotation databases

EnsemblProtistsiEAL63756; EAL63756; DDB_G0287377.
GeneIDi8626108.
KEGGiddi:DDB_G0287377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000100 Genomic DNA. Translation: EAL63756.1.
RefSeqiXP_637277.1. XM_632185.1.

3D structure databases

ProteinModelPortaliQ54KE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0230065.

Proteomic databases

PaxDbiQ54KE6.
PRIDEiQ54KE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL63756; EAL63756; DDB_G0287377.
GeneIDi8626108.
KEGGiddi:DDB_G0287377.

Organism-specific databases

dictyBaseiDDB_G0287377. mccA.

Phylogenomic databases

eggNOGiKOG0238. Eukaryota.
COG4770. LUCA.
InParanoidiQ54KE6.
KOiK01968.
OMAiYQLCARK.
PhylomeDBiQ54KE6.

Enzyme and pathway databases

UniPathwayiUPA00363; UER00861.
ReactomeiR-DDI-196780. Biotin transport and metabolism.
R-DDI-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ54KE6.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCCA_DICDI
AccessioniPrimary (citable) accession number: Q54KE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.