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Q54KB8

- SYEC_DICDI

UniProt

Q54KB8 - SYEC_DICDI

Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gluS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei238 – 2381ATPBy similarity
    Binding sitei264 – 2641GlutamateBy similarity
    Binding sitei422 – 4221GlutamateBy similarity
    Binding sitei425 – 4251ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi460 – 4645ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: dictyBase
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: dictyBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Name:gluS
    Synonyms:gluRS
    ORF Names:DDB_G0287467
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287467. gluS.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 764764Probable glutamate--tRNA ligase, cytoplasmicPRO_0000365601Add
    BLAST

    Proteomic databases

    PRIDEiQ54KB8.

    Expressioni

    Inductioni

    Down-regulated by growth on bacteria.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231321.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54KB8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni228 – 2303Glutamate bindingBy similarity
    Regioni404 – 4085Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi233 – 24210"HIGH" region
    Motifi460 – 4645"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    InParanoidiQ54KB8.
    KOiK01885.
    OMAiTDRNPQY.
    PhylomeDBiQ54KB8.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    1.20.1050.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004046. GST_C.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF14497. GST_C_3. 1 hit.
    PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50405. GST_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54KB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI    50
    VGTQDSLKGS YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE 100
    KFNEFLKEMN EYLTLRAFLI GFNLTLADIV LFARIKMVKE IQEEINKLGK 150
    TIPHLNRWYG YLSQLESFVE ADNAFNGKKE TKASGKAGAE GTAATTEKVA 200
    PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG HCKAAIINNY 250
    YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF 300
    DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF 350
    DEMKKATEQG VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV 400
    YPLYDFACPI VDSVEGITHA LRSNEYNNKR NLYNHYLEIL HLENKPYISD 450
    YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD PRLPTLQGIT RRGLTVAALK 500
    EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK ATAVKFTLSN 550
    GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN 600
    WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV 650
    VLQDYDYIIT KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ 700
    FERRGFFNVD QVGDGVKPYI LIYIPSGPIK PAGAALYPFK KVEKVAAPVN 750
    PKPTAKKQEK QSKK 764
    Length:764
    Mass (Da):85,614
    Last modified:May 24, 2005 - v1
    Checksum:i0627D39B5A8C4801
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000101 Genomic DNA. Translation: EAL63699.1.
    RefSeqiXP_637203.1. XM_632111.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231321; DDB0231321; DDB_G0287467.
    GeneIDi8626140.
    KEGGiddi:DDB_G0287467.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000101 Genomic DNA. Translation: EAL63699.1 .
    RefSeqi XP_637203.1. XM_632111.1.

    3D structure databases

    ProteinModelPortali Q54KB8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231321.

    Proteomic databases

    PRIDEi Q54KB8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231321 ; DDB0231321 ; DDB_G0287467 .
    GeneIDi 8626140.
    KEGGi ddi:DDB_G0287467.

    Organism-specific databases

    dictyBasei DDB_G0287467. gluS.

    Phylogenomic databases

    eggNOGi COG0008.
    InParanoidi Q54KB8.
    KOi K01885.
    OMAi TDRNPQY.
    PhylomeDBi Q54KB8.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    1.20.1050.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004046. GST_C.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF14497. GST_C_3. 1 hit.
    PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50405. GST_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
      Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
      BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYEC_DICDI
    AccessioniPrimary (citable) accession number: Q54KB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 3, 2009
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3