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Q54KB8

- SYEC_DICDI

UniProt

Q54KB8 - SYEC_DICDI

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Protein
Probable glutamate--tRNA ligase, cytoplasmic
Gene
gluS, gluRS, DDB_G0287467
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei238 – 2381ATP By similarity
Binding sitei264 – 2641Glutamate By similarity
Binding sitei422 – 4221Glutamate By similarity
Binding sitei425 – 4251ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi460 – 4645ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. glutamate-tRNA ligase activity Source: dictyBase

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gluS
Synonyms:gluRS
ORF Names:DDB_G0287467
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287467. gluS.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 764764Probable glutamate--tRNA ligase, cytoplasmic
PRO_0000365601Add
BLAST

Proteomic databases

PRIDEiQ54KB8.

Expressioni

Inductioni

Down-regulated by growth on bacteria.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231321.

Structurei

3D structure databases

ProteinModelPortaliQ54KB8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni228 – 2303Glutamate binding By similarity
Regioni404 – 4085Glutamate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi233 – 24210"HIGH" region
Motifi460 – 4645"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiQ54KB8.
KOiK01885.
OMAiTDRNPQY.
PhylomeDBiQ54KB8.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF14497. GST_C_3. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54KB8-1 [UniParc]FASTAAdd to Basket

« Hide

MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI    50
VGTQDSLKGS YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE 100
KFNEFLKEMN EYLTLRAFLI GFNLTLADIV LFARIKMVKE IQEEINKLGK 150
TIPHLNRWYG YLSQLESFVE ADNAFNGKKE TKASGKAGAE GTAATTEKVA 200
PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG HCKAAIINNY 250
YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF 300
DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF 350
DEMKKATEQG VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV 400
YPLYDFACPI VDSVEGITHA LRSNEYNNKR NLYNHYLEIL HLENKPYISD 450
YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD PRLPTLQGIT RRGLTVAALK 500
EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK ATAVKFTLSN 550
GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN 600
WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV 650
VLQDYDYIIT KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ 700
FERRGFFNVD QVGDGVKPYI LIYIPSGPIK PAGAALYPFK KVEKVAAPVN 750
PKPTAKKQEK QSKK 764
Length:764
Mass (Da):85,614
Last modified:May 24, 2005 - v1
Checksum:i0627D39B5A8C4801
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000101 Genomic DNA. Translation: EAL63699.1.
RefSeqiXP_637203.1. XM_632111.1.

Genome annotation databases

EnsemblProtistsiDDB0231321; DDB0231321; DDB_G0287467.
GeneIDi8626140.
KEGGiddi:DDB_G0287467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000101 Genomic DNA. Translation: EAL63699.1 .
RefSeqi XP_637203.1. XM_632111.1.

3D structure databases

ProteinModelPortali Q54KB8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231321.

Proteomic databases

PRIDEi Q54KB8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231321 ; DDB0231321 ; DDB_G0287467 .
GeneIDi 8626140.
KEGGi ddi:DDB_G0287467.

Organism-specific databases

dictyBasei DDB_G0287467. gluS.

Phylogenomic databases

eggNOGi COG0008.
InParanoidi Q54KB8.
KOi K01885.
OMAi TDRNPQY.
PhylomeDBi Q54KB8.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF14497. GST_C_3. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
    Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
    BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYEC_DICDI
AccessioniPrimary (citable) accession number: Q54KB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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