Q54KB8 (SYEC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gluS
Synonyms:	gluRS
ORF Names:	DDB_G0287467

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
Subcellular location	Cytoplasm By similarity.
Induction	Down-regulated by growth on bacteria.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	Probable glutamate--tRNA ligase, cytoplasmic		PRO_0000365601
Regions
	Nucleotide binding	460 – 464	5	ATP By similarity
	Region	228 – 230	3	Glutamate binding By similarity
	Region	404 – 408	5	Glutamate binding By similarity
	Motif	233 – 242	10	"HIGH" region
	Motif	460 – 464	5	"KMSKS" region
Sites
	Binding site	238	1	ATP By similarity
	Binding site	264	1	Glutamate By similarity
	Binding site	422	1	Glutamate By similarity
	Binding site	425	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54KB8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0627D39B5A8C4801	FASTA	764	85,614
	10 20 30 40 50 60 MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI VGTQDSLKGS 70 80 90 100 110 120 YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE KFNEFLKEMN EYLTLRAFLI 130 140 150 160 170 180 GFNLTLADIV LFARIKMVKE IQEEINKLGK TIPHLNRWYG YLSQLESFVE ADNAFNGKKE 190 200 210 220 230 240 TKASGKAGAE GTAATTEKVA PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG 250 260 270 280 290 300 HCKAAIINNY YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF 310 320 330 340 350 360 DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF DEMKKATEQG 370 380 390 400 410 420 VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV YPLYDFACPI VDSVEGITHA 430 440 450 460 470 480 LRSNEYNNKR NLYNHYLEIL HLENKPYISD YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD 490 500 510 520 530 540 PRLPTLQGIT RRGLTVAALK EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK 550 560 570 580 590 600 ATAVKFTLSN GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN 610 620 630 640 650 660 WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV VLQDYDYIIT 670 680 690 700 710 720 KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ FERRGFFNVD QVGDGVKPYI 730 740 750 760 LIYIPSGPIK PAGAALYPFK KVEKVAAPVN PKPTAKKQEK QSKK

References

[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium." Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S. BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000101 Genomic DNA. Translation: EAL63699.1.
RefSeq	XP_637203.1. XM_632111.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYL based on UniProtKB P00962.
ProteinModelPortal	Q54KB8.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231321.
Proteomic databases
PRIDE	Q54KB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231321; DDB0231321; DDB_G0287467.
GeneID	8626140.
KEGG	ddi:DDB_G0287467.
Organism-specific databases
dictyBase	DDB_G0287467. gluS.
Phylogenomic databases
eggNOG	COG0008.
InParanoid	Q54KB8.
KO	K01885.
OMA	QWLDYAP.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 1.20.1050.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004526. Glu-tRNA-synth_Ib_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50405. GST_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYEC_DICDI

Accession

Primary (citable) accession number: Q54KB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)