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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gluS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei238ATPBy similarity1
Binding sitei264GlutamateBy similarity1
Binding sitei422GlutamateBy similarity1
Binding sitei425ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi460 – 464ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gluS
Synonyms:gluRS
ORF Names:DDB_G0287467
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287467 gluS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003656011 – 764Probable glutamate--tRNA ligase, cytoplasmicAdd BLAST764

Proteomic databases

PaxDbiQ54KB8

Expressioni

Inductioni

Down-regulated by growth on bacteria.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231321

Structurei

3D structure databases

ProteinModelPortaliQ54KB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni228 – 230Glutamate bindingBy similarity3
Regioni404 – 408Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi233 – 242"HIGH" region10
Motifi460 – 464"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1147 Eukaryota
COG0008 LUCA
InParanoidiQ54KB8
KOiK01885
OMAiFARMNFI
PhylomeDBiQ54KB8

Family and domain databases

Gene3Di2.40.240.10, 3 hits
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004046 GST_C
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50405 GST_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q54KB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI
60 70 80 90 100
VGTQDSLKGS YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE
110 120 130 140 150
KFNEFLKEMN EYLTLRAFLI GFNLTLADIV LFARIKMVKE IQEEINKLGK
160 170 180 190 200
TIPHLNRWYG YLSQLESFVE ADNAFNGKKE TKASGKAGAE GTAATTEKVA
210 220 230 240 250
PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG HCKAAIINNY
260 270 280 290 300
YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF
310 320 330 340 350
DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF
360 370 380 390 400
DEMKKATEQG VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV
410 420 430 440 450
YPLYDFACPI VDSVEGITHA LRSNEYNNKR NLYNHYLEIL HLENKPYISD
460 470 480 490 500
YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD PRLPTLQGIT RRGLTVAALK
510 520 530 540 550
EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK ATAVKFTLSN
560 570 580 590 600
GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN
610 620 630 640 650
WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV
660 670 680 690 700
VLQDYDYIIT KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ
710 720 730 740 750
FERRGFFNVD QVGDGVKPYI LIYIPSGPIK PAGAALYPFK KVEKVAAPVN
760
PKPTAKKQEK QSKK
Length:764
Mass (Da):85,614
Last modified:May 24, 2005 - v1
Checksum:i0627D39B5A8C4801
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000101 Genomic DNA Translation: EAL63699.1
RefSeqiXP_637203.1, XM_632111.1

Genome annotation databases

EnsemblProtistsiEAL63699; EAL63699; DDB_G0287467
GeneIDi8626140
KEGGiddi:DDB_G0287467

Similar proteinsi

Entry informationi

Entry nameiSYEC_DICDI
AccessioniPrimary (citable) accession number: Q54KB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families
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