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Q54KB8

- SYEC_DICDI

UniProt

Q54KB8 - SYEC_DICDI

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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gluS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei238 – 2381ATPBy similarity
Binding sitei264 – 2641GlutamateBy similarity
Binding sitei422 – 4221GlutamateBy similarity
Binding sitei425 – 4251ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi460 – 4645ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: dictyBase
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gluS
Synonyms:gluRS
ORF Names:DDB_G0287467
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287467. gluS.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 764764Probable glutamate--tRNA ligase, cytoplasmicPRO_0000365601Add
BLAST

Proteomic databases

PRIDEiQ54KB8.

Expressioni

Inductioni

Down-regulated by growth on bacteria.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231321.

Structurei

3D structure databases

ProteinModelPortaliQ54KB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni228 – 2303Glutamate bindingBy similarity
Regioni404 – 4085Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi233 – 24210"HIGH" region
Motifi460 – 4645"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiQ54KB8.
KOiK01885.
OMAiTDRNPQY.
PhylomeDBiQ54KB8.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF14497. GST_C_3. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54KB8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI
60 70 80 90 100
VGTQDSLKGS YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE
110 120 130 140 150
KFNEFLKEMN EYLTLRAFLI GFNLTLADIV LFARIKMVKE IQEEINKLGK
160 170 180 190 200
TIPHLNRWYG YLSQLESFVE ADNAFNGKKE TKASGKAGAE GTAATTEKVA
210 220 230 240 250
PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG HCKAAIINNY
260 270 280 290 300
YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF
310 320 330 340 350
DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF
360 370 380 390 400
DEMKKATEQG VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV
410 420 430 440 450
YPLYDFACPI VDSVEGITHA LRSNEYNNKR NLYNHYLEIL HLENKPYISD
460 470 480 490 500
YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD PRLPTLQGIT RRGLTVAALK
510 520 530 540 550
EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK ATAVKFTLSN
560 570 580 590 600
GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN
610 620 630 640 650
WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV
660 670 680 690 700
VLQDYDYIIT KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ
710 720 730 740 750
FERRGFFNVD QVGDGVKPYI LIYIPSGPIK PAGAALYPFK KVEKVAAPVN
760
PKPTAKKQEK QSKK
Length:764
Mass (Da):85,614
Last modified:May 24, 2005 - v1
Checksum:i0627D39B5A8C4801
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000101 Genomic DNA. Translation: EAL63699.1.
RefSeqiXP_637203.1. XM_632111.1.

Genome annotation databases

EnsemblProtistsiDDB0231321; DDB0231321; DDB_G0287467.
GeneIDi8626140.
KEGGiddi:DDB_G0287467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000101 Genomic DNA. Translation: EAL63699.1 .
RefSeqi XP_637203.1. XM_632111.1.

3D structure databases

ProteinModelPortali Q54KB8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231321.

Proteomic databases

PRIDEi Q54KB8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231321 ; DDB0231321 ; DDB_G0287467 .
GeneIDi 8626140.
KEGGi ddi:DDB_G0287467.

Organism-specific databases

dictyBasei DDB_G0287467. gluS.

Phylogenomic databases

eggNOGi COG0008.
InParanoidi Q54KB8.
KOi K01885.
OMAi TDRNPQY.
PhylomeDBi Q54KB8.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF14497. GST_C_3. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
    Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
    BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYEC_DICDI
AccessioniPrimary (citable) accession number: Q54KB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 24, 2005
Last modified: October 1, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3