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Q54KB8 (SYEC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamate--tRNA ligase, cytoplasmic

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gluS
Synonyms:gluRS
ORF Names:DDB_G0287467
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Subcellular location

Cytoplasm By similarity.

Induction

Down-regulated by growth on bacteria.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Probable glutamate--tRNA ligase, cytoplasmic
PRO_0000365601

Regions

Nucleotide binding460 – 4645ATP By similarity
Region228 – 2303Glutamate binding By similarity
Region404 – 4085Glutamate binding By similarity
Motif233 – 24210"HIGH" region
Motif460 – 4645"KMSKS" region

Sites

Binding site2381ATP By similarity
Binding site2641Glutamate By similarity
Binding site4221Glutamate By similarity
Binding site4251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54KB8 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 0627D39B5A8C4801

FASTA76485,614
        10         20         30         40         50         60 
MSKAKDTGIL RFDDTPLAAT FPLVAIITSK VVGGVKIVGR KGLDSTEFSI VGTQDSLKGS 

        70         80         90        100        110        120 
YVIAKYLART TPSLSLYGEN ALSASKIDEF IDKFAHLKSE KFNEFLKEMN EYLTLRAFLI 

       130        140        150        160        170        180 
GFNLTLADIV LFARIKMVKE IQEEINKLGK TIPHLNRWYG YLSQLESFVE ADNAFNGKKE 

       190        200        210        220        230        240 
TKASGKAGAE GTAATTEKVA PQKGAMGWVG NFEALNLPGL VEGKVVTRFP PEPSGYMHIG 

       250        260        270        280        290        300 
HCKAAIINNY YAEKYNGKII IRFDDTNPSK EKEEYVENII KDINNLGIKY EKITHTSDYF 

       310        320        330        340        350        360 
DLIHDYAIQM IKEGIAYCDD TPQVKMSEER DNAIESVHRN NSVEKNLEMF DEMKKATEQG 

       370        380        390        400        410        420 
VKCVLRAKLD MAHIDKAFRD PAIYRCNSTP HHRTGDKYKV YPLYDFACPI VDSVEGITHA 

       430        440        450        460        470        480 
LRSNEYNNKR NLYNHYLEIL HLENKPYISD YSRLSFFNVL LSKRKLQHFV DTGLVSGWTD 

       490        500        510        520        530        540 
PRLPTLQGIT RRGLTVAALK EFILSQGASA ANTTLDLGKL FVGNKAVLEP TCPRYTAIAK 

       550        560        570        580        590        600 
ATAVKFTLSN GPTLPEVKDC LKYAKDPSMG TKKVTFSNNL LLEGDDCNQI KEGEEVTLMN 

       610        620        630        640        650        660 
WGNAIVETLQ RNENGDVVSM TGKLHLEGDV KKTDKKLSWL SSDCADTVTV VLQDYDYIIT 

       670        680        690        700        710        720 
KPKLEDGDDL DTFTNKNSKF EIEAFTDENI LTLKLNDKIQ FERRGFFNVD QVGDGVKPYI 

       730        740        750        760 
LIYIPSGPIK PAGAALYPFK KVEKVAAPVN PKPTAKKQEK QSKK 

« Hide

References

[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000101 Genomic DNA. Translation: EAL63699.1.

3D structure databases

ProteinModelPortalQ54KB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231321.

Proteomic databases

PRIDEQ54KB8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231321; DDB0231321; DDB_G0287467.
KEGGddi:DDB_G0287467.

Organism-specific databases

dictyBaseDDB_G0287467. gluS.

Phylogenomic databases

eggNOGCOG0008.
InParanoidQ54KB8.
KOK01885.
OMAFARMNFI.
PhylomeDBQ54KB8.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYEC_DICDI
AccessionPrimary (citable) accession number: Q54KB8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries