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Protein

Glutamate dehydrogenase, mitochondrial

Gene

gluD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Enzyme regulationi

Subject to allosteric regulation. Activated by AMP and ADP.2 Publications

Kineticsi

  1. KM=0.36 mM for alpha-ketoglutarate1 Publication
  2. KM=16 µM for NADH1 Publication
  3. KM=34.5 mM for NH31 Publication

    pH dependencei

    Optimum pH is 7.25 to 7.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei102 – 1021SubstrateBy similarity
    Binding sitei126 – 1261SubstrateBy similarity
    Binding sitei131 – 1311NADBy similarity
    Active sitei138 – 1381PROSITE-ProRule annotation
    Binding sitei394 – 3941SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi96 – 983NADBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    ATP-binding, NAD, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_313802. Amino acid synthesis and interconversion (transamination).
    SABIO-RKQ54KB7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate dehydrogenase, mitochondrial (EC:1.4.1.3)
    Short name:
    GDH
    Gene namesi
    Name:gluD
    ORF Names:DDB_G0287469
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287469. glud1.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrial matrix Source: UniProtKB-SubCell
    • mitochondrion Source: dictyBase
    • phagocytic vesicle Source: dictyBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 502Glutamate dehydrogenase, mitochondrialPRO_0000327666
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    PRIDEiQ54KB7.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    STRINGi44689.DDB0231438.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54KB7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0334.
    InParanoidiQ54KB7.
    KOiK00261.
    OMAiCDICIPA.
    PhylomeDBiQ54KB7.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54KB7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQSLARLSRT SLVQKGLVPQ TIKNYSSVSQ AEIDNEPRFL ECFKTFFDKA
    60 70 80 90 100
    AGLTNLKPGV LNNMKECNVA LRVEFPIKNE HGDVDIIAGY RAQHSHHRLP
    110 120 130 140 150
    CKGGIRFSEE VDLQEVMALA SLMTYKCAVV DVPFGGAKGG VRIDPKKYTV
    160 170 180 190 200
    AQREKITRAY TLLLCQKNFI GPGVDVPAPD MGTGEQEMAW IRDTYQAFNT
    210 220 230 240 250
    NDVDSMACVT GKPISSGGIR GRTEATGLGV FYGIREFLSY EEVLKKTGLT
    260 270 280 290 300
    PGIKGKSIVI QGFGNVGYFA AKFFEQAGAK VIAVAEHNGA VYNADGLNID
    310 320 330 340 350
    ALNKYKLQHG TFIDFPGATN IVDSVKALEI PCDILIPAAL EKQIHIGNVA
    360 370 380 390 400
    DIQAKLIGEA ANGPMTPRAD QILLNRGHVI IPDLLLNAGG VTVSYFEWLK
    410 420 430 440 450
    NLSHVRFGRL NKKWEESSKK LLLEFVESTV NKKLSEAERS LIIHGADEID
    460 470 480 490 500
    IVRSGLEDTM QNACAETRKT ANEKNTDYRS AALYNAIMKI KAVYESSGNV

    FS
    Length:502
    Mass (Da):55,042
    Last modified:May 24, 2005 - v1
    Checksum:iD6BB324A859C0528
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAFI02000101 Genomic DNA. Translation: EAL63700.1.
    RefSeqiXP_637204.1. XM_632112.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231438; DDB0231438; DDB_G0287469.
    GeneIDi8626141.
    KEGGiddi:DDB_G0287469.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAFI02000101 Genomic DNA. Translation: EAL63700.1.
    RefSeqiXP_637204.1. XM_632112.1.

    3D structure databases

    ProteinModelPortaliQ54KB7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi44689.DDB0231438.

    Proteomic databases

    PRIDEiQ54KB7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0231438; DDB0231438; DDB_G0287469.
    GeneIDi8626141.
    KEGGiddi:DDB_G0287469.

    Organism-specific databases

    dictyBaseiDDB_G0287469. glud1.

    Phylogenomic databases

    eggNOGiCOG0334.
    InParanoidiQ54KB7.
    KOiK00261.
    OMAiCDICIPA.
    PhylomeDBiQ54KB7.

    Enzyme and pathway databases

    ReactomeiREACT_313802. Amino acid synthesis and interconversion (transamination).
    SABIO-RKQ54KB7.

    Miscellaneous databases

    PROiQ54KB7.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The NAD-dependent glutamate dehydrogenase from Dictyostelium discoideum: purification and properties."
      Pamula F., Wheldrake J.F.
      Arch. Biochem. Biophys. 291:225-230(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
    3. "The effect of AMP on the NAD-dependent glutamate dehydrogenase during activation and morphogenesis in the cellular slime moulds."
      Pamula F., Wheldrake J.F.
      J. Gen. Microbiol. 138:1935-1940(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiDHE3_DICDI
    AccessioniPrimary (citable) accession number: Q54KB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: July 22, 2015
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    ADP can occupy the NADH binding site and activate the enzyme.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.