Q54K67 (MANG_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-mannosidase G
EC=3.2.1.24

Gene names

Name:	manG
ORF Names:	DDB_G0287577

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1087 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mannose metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-mannosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1087	1087	Alpha-mannosidase G		PRO_0000327486
Regions
	Compositional bias	628 – 641	14	Poly-Thr
Sites
	Active site	376	1	Nucleophile By similarity
	Metal binding	264	1	Zinc By similarity
	Metal binding	266	1	Zinc By similarity
	Metal binding	376	1	Zinc By similarity
	Metal binding	579	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54K67 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 32E15E3EA33F6CAA	FASTA	1,087	123,691
	10 20 30 40 50 60 MTSGNVMLKH QDVTIERIEK FLSDTYFVRE NLYGKLISLK SSEAVKVKVS PKVEGISYKD 70 80 90 100 110 120 AIQLEYKDTK IGESFGPSWT NYWFKVTIDV PTDWKDKTIH FIWNSSCEGL IWMNGIAIQG 130 140 150 160 170 180 LIGGTWQDLR EEYKLIENSK GGEHFEFYIE ISCNGMFGVG KDGLINPCDP DRTFELTKAE 190 200 210 220 230 240 IRVKNKEANE LYMYLQMLYD VGKNFPKESL RKKQAIWVAN DIINQCNVND SRTFSKCIEL 250 260 270 280 290 300 AKKEFFSQHN SESQTRVWAV GHCHIDLCWL WSFEKTKEKC ARSFSTQILY MDYYPQFKFT 310 320 330 340 350 360 QSQAQAYQWT KENYPELYER IKEKVVTGQF IPTGGTWVEM DGNLPSGESF IRQFLYGQRF 370 380 390 400 410 420 FEKEFGKKCT EFFLPDTFGY SAQLPQVIRH MGIENFITQK LSWNNLNKFP HSTFIWEGID 430 440 450 460 470 480 GSSVLTHFPP ADTYNSQADV KEIVMSSSNN KDIDRCNESM LLYGNGDGGG GPTIPMIERL 490 500 510 520 530 540 TILKDTAGIP KIEFSTPAQF FKQLEPHRSK LNKWVGELYF ELHRGTYTSQ ATTKRGNRLC 550 560 570 580 590 600 EIELHATEML TSYCELFVEG FKSPNLSKLW QQVLLCQFHD ALPGSSIQVC YEDILKIHQQ 610 620 630 640 650 660 VLVECKNIIT QSMNHITGTL LKIDNLPTTS TTTSTTTTST TECTKNSEFV LAFNANDFEI 670 680 690 700 710 720 SRVIEIPKSN KDIQAQYINA IQTSYNGLPL GTVSLPPNGF SAINISTSGD NRTINRKPGY 730 740 750 760 770 780 PCTAIEKNDA SGDILIDNQF ISIVIGSNGR IKSLIEKSAN REVIKQDGSL GNRLIIFDDT 790 800 810 820 830 840 CLFWDAWDQE IFSLEKPLSI LEGTCKIIEN GPLRCVVQVH YDSKGLPSGN GSVNQTIIVH 850 860 870 880 890 900 FNSARVDFET NVNWNEAHKL LRVDFDTNIR AKNANYEIQF GHIERPTHYN TSWDFARFEV 910 920 930 940 950 960 VGHKWADLSE YDFGMALLND CKYGYSTLGG RIGLSLLRSP KSPDDTCDMG SHKFTYSIYP 970 980 990 1000 1010 1020 HRGSLQSASV IKEGYSLNNN FYISETPFSL ASTTHIDKTF ISTNKEAIIV DTIKKAEDGT 1030 1040 1050 1060 1070 1080 SFVVRVYESF GGATTFNFTS SILPIPFKSI IECNGLEEVN QSSKSYKFND TIKINPFEIK TFRFISN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 863-870 AND 932-938, MASS SPECTROMETRY. Strain: AX2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000102 Genomic DNA. Translation: EAL63688.1.
RefSeq	XP_637188.1. XM_632096.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231611.
Proteomic databases
PRIDE	Q54K67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231611; DDB0231611; DDB_G0287577.
GeneID	8626190.
KEGG	ddi:DDB_G0287577.
Organism-specific databases
dictyBase	DDB_G0287577. manG.
Phylogenomic databases
eggNOG	COG0383.
KO	K01191.
OMA	DPEKMFQ.
ProtClustDB	CLSZ2728867.
Family and domain databases
Gene3D	3.20.110.10. 1 hit.
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR027291. Glyco_hydro_38/57_N. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view]
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANG_DICDI

Accession

Primary (citable) accession number: Q54K67

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)