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Q54K57

- TPSA_DICDI

UniProt

Q54K57 - TPSA_DICDI

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A

Gene

tpsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination.1 Publication

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A (EC:2.4.1.15)
    Alternative name(s):
    Trehalose-6-phosphate synthase A
    UDP-glucose-glucosephosphate glucosyltransferase A
    Gene namesi
    Name:tpsA
    ORF Names:DDB_G0287657
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287657. tpsA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 733733Alpha,alpha-trehalose-phosphate synthase [UDP-forming] APRO_0000328410Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed during aggregation, reaching a peak at 12-16 hours. Accumulates in prestalk cells and, to a lesser extent, in prespore cells as well.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231979.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54K57.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
    In the C-terminal section; belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    KOiK16055.
    OMAiWHYRTAD.
    PhylomeDBiQ54K57.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR012766. Trehalose_OtsA.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR00685. T6PP. 1 hit.
    TIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54K57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNENDNNLEN ETGFSGRLIV VSNRLPVSIK KESNGKWSCK MSSGGLVAAL    50
    SGLKSNFIWV GWIGAEIEED DRKEIKELLW KDYSCIPVFL SEKVANEHYN 100
    GFSNGVLWPL FHYLPGDLDY DDRIWNSYVE ANEQFSSVVA EILKPNDLVW 150
    VHDYHMMLLP EILKQKKPDA RIGFFLHIPF PSSEIFRVLP CRKEILLGIL 200
    NCCLIGFHTY DYARHFLKSC TRIVGLETAP NGVYFKDRFV QVGVFPVGID 250
    PDKFFESLKT TQVQNRIKEL KESFEGTKVL IGIDRLDYIK GIPQKLQAIE 300
    RLFQKYPEWK GKLVLIQVAV PSRQDVEEYQ KLKKEVEELV GRINGLYGSI 350
    GYSPIHYLFQ SVDPSELTAL YNISDAALIT SIRDGMNLVA QEYIVCQTEN 400
    NGVLILSEFT GAAQSLSGAV MINPWNTEEV ADSIHNSLLM PPEEREEKHQ 450
    MLLKYVTKHT ASHWGLGFVK ELNKASSNTD KMVTIQKLDI EKVVDIYKQS 500
    KRRLLIFAYD GTLIPYNNVP QLSRPSQELL NSFDILSNDP KTDVYILSGR 550
    DKKTLSEWFL GIQIGLSAEY GCFFKLPEST EWEQQVPSMD LSWKETIRPL 600
    FKYFTLRTPG SFFEEKEMLF TWHYRNADPI FGSIQARELH LHLDNLPLDV 650
    IVGDKTLGVR SYNINPLSSM KKVITDTIPK GLDLILLIGD THIHPSELPT 700
    FDGKIFNISV GKKSVKDSYH LSDPAEVNYL IIN 733
    Length:733
    Mass (Da):83,717
    Last modified:May 24, 2005 - v1
    Checksum:iC15766757DE3E081
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000103 Genomic DNA. Translation: EAL63637.1.
    RefSeqiXP_637107.1. XM_632015.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231979; DDB0231979; DDB_G0287657.
    GeneIDi8626202.
    KEGGiddi:DDB_G0287657.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000103 Genomic DNA. Translation: EAL63637.1 .
    RefSeqi XP_637107.1. XM_632015.1.

    3D structure databases

    ProteinModelPortali Q54K57.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231979.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231979 ; DDB0231979 ; DDB_G0287657 .
    GeneIDi 8626202.
    KEGGi ddi:DDB_G0287657.

    Organism-specific databases

    dictyBasei DDB_G0287657. tpsA.

    Phylogenomic databases

    eggNOGi COG0380.
    KOi K16055.
    OMAi WHYRTAD.
    PhylomeDBi Q54K57.

    Miscellaneous databases

    PROi Q54K57.

    Family and domain databases

    Gene3Di 3.40.50.1000. 2 hits.
    InterProi IPR001830. Glyco_trans_20.
    IPR023214. HAD-like_dom.
    IPR012766. Trehalose_OtsA.
    IPR003337. Trehalose_PPase.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    PF02358. Trehalose_PPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR00685. T6PP. 1 hit.
    TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps."
      Killick K.A.
      Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Expression patterns of cell-type-specific genes in Dictyostelium."
      Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F.
      Mol. Biol. Cell 12:2590-2600(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
      Strain: NC-4.

    Entry informationi

    Entry nameiTPSA_DICDI
    AccessioniPrimary (citable) accession number: Q54K57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3