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Q54K57 (TPSA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A

EC=2.4.1.15
Alternative name(s):
Trehalose-6-phosphate synthase A
UDP-glucose-glucosephosphate glucosyltransferase A
Gene names
Name:tpsA
ORF Names:DDB_G0287657
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length733 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination. Ref.2

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Developmental stage

Expressed during aggregation, reaching a peak at 12-16 hours. Accumulates in prestalk cells and, to a lesser extent, in prespore cells as well. Ref.3

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 20 family.

In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 733733Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A
PRO_0000328410

Sequences

Sequence LengthMass (Da)Tools
Q54K57 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: C15766757DE3E081

FASTA73383,717
        10         20         30         40         50         60 
MNENDNNLEN ETGFSGRLIV VSNRLPVSIK KESNGKWSCK MSSGGLVAAL SGLKSNFIWV 

        70         80         90        100        110        120 
GWIGAEIEED DRKEIKELLW KDYSCIPVFL SEKVANEHYN GFSNGVLWPL FHYLPGDLDY 

       130        140        150        160        170        180 
DDRIWNSYVE ANEQFSSVVA EILKPNDLVW VHDYHMMLLP EILKQKKPDA RIGFFLHIPF 

       190        200        210        220        230        240 
PSSEIFRVLP CRKEILLGIL NCCLIGFHTY DYARHFLKSC TRIVGLETAP NGVYFKDRFV 

       250        260        270        280        290        300 
QVGVFPVGID PDKFFESLKT TQVQNRIKEL KESFEGTKVL IGIDRLDYIK GIPQKLQAIE 

       310        320        330        340        350        360 
RLFQKYPEWK GKLVLIQVAV PSRQDVEEYQ KLKKEVEELV GRINGLYGSI GYSPIHYLFQ 

       370        380        390        400        410        420 
SVDPSELTAL YNISDAALIT SIRDGMNLVA QEYIVCQTEN NGVLILSEFT GAAQSLSGAV 

       430        440        450        460        470        480 
MINPWNTEEV ADSIHNSLLM PPEEREEKHQ MLLKYVTKHT ASHWGLGFVK ELNKASSNTD 

       490        500        510        520        530        540 
KMVTIQKLDI EKVVDIYKQS KRRLLIFAYD GTLIPYNNVP QLSRPSQELL NSFDILSNDP 

       550        560        570        580        590        600 
KTDVYILSGR DKKTLSEWFL GIQIGLSAEY GCFFKLPEST EWEQQVPSMD LSWKETIRPL 

       610        620        630        640        650        660 
FKYFTLRTPG SFFEEKEMLF TWHYRNADPI FGSIQARELH LHLDNLPLDV IVGDKTLGVR 

       670        680        690        700        710        720 
SYNINPLSSM KKVITDTIPK GLDLILLIGD THIHPSELPT FDGKIFNISV GKKSVKDSYH 

       730 
LSDPAEVNYL IIN 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps."
Killick K.A.
Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Expression patterns of cell-type-specific genes in Dictyostelium."
Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F.
Mol. Biol. Cell 12:2590-2600(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
Strain: NC-4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000103 Genomic DNA. Translation: EAL63637.1.
RefSeqXP_637107.1. XM_632015.1.

3D structure databases

ProteinModelPortalQ54K57.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231979.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231979; DDB0231979; DDB_G0287657.
GeneID8626202.
KEGGddi:DDB_G0287657.

Organism-specific databases

dictyBaseDDB_G0287657. tpsA.

Phylogenomic databases

eggNOGCOG0380.
OMAWHYRTAD.
PhylomeDBQ54K57.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
InterProIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR012766. Trehalose_OtsA.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR00685. T6PP. 1 hit.
TIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Other

PROQ54K57.

Entry information

Entry nameTPSA_DICDI
AccessionPrimary (citable) accession number: Q54K57
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase