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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A

Gene

tpsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination.1 Publication

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase A
UDP-glucose-glucosephosphate glucosyltransferase A
Gene namesi
Name:tpsA
ORF Names:DDB_G0287657
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287657. tpsA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733Alpha,alpha-trehalose-phosphate synthase [UDP-forming] APRO_0000328410Add
BLAST

Expressioni

Developmental stagei

Expressed during aggregation, reaching a peak at 12-16 hours. Accumulates in prestalk cells and, to a lesser extent, in prespore cells as well.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231979.

Structurei

3D structure databases

ProteinModelPortaliQ54K57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the trehalose phosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0380.
InParanoidiQ54K57.
KOiK16055.
OMAiIWIGWPG.
PhylomeDBiQ54K57.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR012766. Trehalose_OtsA.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR00685. T6PP. 1 hit.
TIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54K57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNENDNNLEN ETGFSGRLIV VSNRLPVSIK KESNGKWSCK MSSGGLVAAL
60 70 80 90 100
SGLKSNFIWV GWIGAEIEED DRKEIKELLW KDYSCIPVFL SEKVANEHYN
110 120 130 140 150
GFSNGVLWPL FHYLPGDLDY DDRIWNSYVE ANEQFSSVVA EILKPNDLVW
160 170 180 190 200
VHDYHMMLLP EILKQKKPDA RIGFFLHIPF PSSEIFRVLP CRKEILLGIL
210 220 230 240 250
NCCLIGFHTY DYARHFLKSC TRIVGLETAP NGVYFKDRFV QVGVFPVGID
260 270 280 290 300
PDKFFESLKT TQVQNRIKEL KESFEGTKVL IGIDRLDYIK GIPQKLQAIE
310 320 330 340 350
RLFQKYPEWK GKLVLIQVAV PSRQDVEEYQ KLKKEVEELV GRINGLYGSI
360 370 380 390 400
GYSPIHYLFQ SVDPSELTAL YNISDAALIT SIRDGMNLVA QEYIVCQTEN
410 420 430 440 450
NGVLILSEFT GAAQSLSGAV MINPWNTEEV ADSIHNSLLM PPEEREEKHQ
460 470 480 490 500
MLLKYVTKHT ASHWGLGFVK ELNKASSNTD KMVTIQKLDI EKVVDIYKQS
510 520 530 540 550
KRRLLIFAYD GTLIPYNNVP QLSRPSQELL NSFDILSNDP KTDVYILSGR
560 570 580 590 600
DKKTLSEWFL GIQIGLSAEY GCFFKLPEST EWEQQVPSMD LSWKETIRPL
610 620 630 640 650
FKYFTLRTPG SFFEEKEMLF TWHYRNADPI FGSIQARELH LHLDNLPLDV
660 670 680 690 700
IVGDKTLGVR SYNINPLSSM KKVITDTIPK GLDLILLIGD THIHPSELPT
710 720 730
FDGKIFNISV GKKSVKDSYH LSDPAEVNYL IIN
Length:733
Mass (Da):83,717
Last modified:May 24, 2005 - v1
Checksum:iC15766757DE3E081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000103 Genomic DNA. Translation: EAL63637.1.
RefSeqiXP_637107.1. XM_632015.1.

Genome annotation databases

EnsemblProtistsiDDB0231979; DDB0231979; DDB_G0287657.
GeneIDi8626202.
KEGGiddi:DDB_G0287657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000103 Genomic DNA. Translation: EAL63637.1.
RefSeqiXP_637107.1. XM_632015.1.

3D structure databases

ProteinModelPortaliQ54K57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231979; DDB0231979; DDB_G0287657.
GeneIDi8626202.
KEGGiddi:DDB_G0287657.

Organism-specific databases

dictyBaseiDDB_G0287657. tpsA.

Phylogenomic databases

eggNOGiCOG0380.
InParanoidiQ54K57.
KOiK16055.
OMAiIWIGWPG.
PhylomeDBiQ54K57.

Miscellaneous databases

PROiQ54K57.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR012766. Trehalose_OtsA.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR00685. T6PP. 1 hit.
TIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps."
    Killick K.A.
    Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Expression patterns of cell-type-specific genes in Dictyostelium."
    Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F.
    Mol. Biol. Cell 12:2590-2600(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
    Strain: NC-4.

Entry informationi

Entry nameiTPSA_DICDI
AccessioniPrimary (citable) accession number: Q54K57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.