Q54K57 (TPSA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase A
UDP-glucose-glucosephosphate glucosyltransferase A

Gene names

Name:	tpsA
ORF Names:	DDB_G0287657

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesizes trehalose 6-phosphate, the precursor for the production of trehalose, the main carbohydrate storage reserve of the dormant spore. Trehalose accumulates in both prestalk and prespore cells and then is rapidly metabolized during terminal differentiation of stalk cells, while being stored in spores, where it serves as the principal energy and carbon source for germination. Ref.2
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Developmental stage	Expressed during aggregation, reaching a peak at 12-16 hours. Accumulates in prestalk cells and, to a lesser extent, in prespore cells as well. Ref.3
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A		PRO_0000328410

Sequences

	Sequence		Length	Mass (Da)
	Q54K57 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C15766757DE3E081	FASTA	733	83,717
	10 20 30 40 50 60 MNENDNNLEN ETGFSGRLIV VSNRLPVSIK KESNGKWSCK MSSGGLVAAL SGLKSNFIWV 70 80 90 100 110 120 GWIGAEIEED DRKEIKELLW KDYSCIPVFL SEKVANEHYN GFSNGVLWPL FHYLPGDLDY 130 140 150 160 170 180 DDRIWNSYVE ANEQFSSVVA EILKPNDLVW VHDYHMMLLP EILKQKKPDA RIGFFLHIPF 190 200 210 220 230 240 PSSEIFRVLP CRKEILLGIL NCCLIGFHTY DYARHFLKSC TRIVGLETAP NGVYFKDRFV 250 260 270 280 290 300 QVGVFPVGID PDKFFESLKT TQVQNRIKEL KESFEGTKVL IGIDRLDYIK GIPQKLQAIE 310 320 330 340 350 360 RLFQKYPEWK GKLVLIQVAV PSRQDVEEYQ KLKKEVEELV GRINGLYGSI GYSPIHYLFQ 370 380 390 400 410 420 SVDPSELTAL YNISDAALIT SIRDGMNLVA QEYIVCQTEN NGVLILSEFT GAAQSLSGAV 430 440 450 460 470 480 MINPWNTEEV ADSIHNSLLM PPEEREEKHQ MLLKYVTKHT ASHWGLGFVK ELNKASSNTD 490 500 510 520 530 540 KMVTIQKLDI EKVVDIYKQS KRRLLIFAYD GTLIPYNNVP QLSRPSQELL NSFDILSNDP 550 560 570 580 590 600 KTDVYILSGR DKKTLSEWFL GIQIGLSAEY GCFFKLPEST EWEQQVPSMD LSWKETIRPL 610 620 630 640 650 660 FKYFTLRTPG SFFEEKEMLF TWHYRNADPI FGSIQARELH LHLDNLPLDV IVGDKTLGVR 670 680 690 700 710 720 SYNINPLSSM KKVITDTIPK GLDLILLIGD THIHPSELPT FDGKIFNISV GKKSVKDSYH 730 LSDPAEVNYL IIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps." Killick K.A. Arch. Biochem. Biophys. 196:121-133(1979) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Expression patterns of cell-type-specific genes in Dictyostelium." Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F. Mol. Biol. Cell 12:2590-2600(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: NC-4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000103 Genomic DNA. Translation: EAL63637.1.
RefSeq	XP_637107.1. XM_632015.1.
3D structure databases
ProteinModelPortal	Q54K57.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231979; DDB0231979; DDB_G0287657.
GeneID	8626202.
KEGG	ddi:DDB_G0287657.
Organism-specific databases
dictyBase	DDB_G0287657. tpsA.
Phylogenomic databases
eggNOG	COG0380.
KO	K16055.
OMA	SLAWHYR.
ProtClustDB	CLSZ2728860.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR012766. Trehalose_OtsA. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR00685. T6PP. 1 hit. TIGR02400. trehalose_OtsA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPSA_DICDI

Accession

Primary (citable) accession number: Q54K57

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)