Reviewed, UniProtKB/Swiss-Prot Q54K56 (HEXB2_DICDI)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit B2
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit B2
    Beta-N-acetylhexosaminidase subunit B2

Gene names

Name:	hexb2
ORF Names:	DDB_G0287659

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 564	545	Beta-hexosaminidase subunit B2		PRO_0000331238
Regions
	Compositional bias	45 – 54	10	Poly-Ser
Sites
	Active site	357	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	377	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	524	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54K56-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0C65D857428ABC9E	FASTA	564	64,566
	10 20 30 40 50 60 MKLKFIFLIL FFIIGNSIGI KISKEINKIK LNDISIDGEI LLNKSSDSSS SQSSKIINIW 70 80 90 100 110 120 PMPKKVLNGD ITVYISPHFQ FTTNLTKSTT LKKAMDRYYK LIFTEDSKSH SGISILNEIK 130 140 150 160 170 180 ILVKSEDETL QIGFDESYEI YIDDSGDDGG KIIAETVYGA IRGLETLYQM IGFDYQREYY 190 200 210 220 230 240 QIKHCPWIIQ DSPRYPHRGV MLDTSRHFYS VDVLKEFIEA LAYNKFNVFH WHAVDSQSFP 250 260 270 280 290 300 LTSTTFPKIT KGSWSSQEIY STRDIKEIIQ HAKEYGIRVE LEIDMPGHAY SWGIGYPSVL 310 320 330 340 350 360 PANFSHSIQC QQPCPTECNI PLDVSSKESY VIAMGLLEEF NGASMFNESF FHIGGDEVAY 370 380 390 400 410 420 SCWNNSLRIV DWMKRENISS FQDAAIFFEI KAIEQLIQLG KTPVMWEDAY LLFGSSGITE 430 440 450 460 470 480 KLPEEVVVQI YHDPLLALNT TRDGYKTLQS PYWPYYLDNP SVDWEKVYEF EPSNGIHEKR 490 500 510 520 530 540 LRLLLGGETC MWSELVDASN LFAKVFPRAF ATAERLWFSI ENSNSTTFAK PRLERFRCFL 550 560 LERGIGAAPL NSTSPDDPNS CYSS

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000103 Genomic DNA. Translation: EAL63638.1.
RefSeq	XP_637108.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54K56.
Genome annotation databases
GeneID	3388682.
GenomeReviews	Gene locus hexb2 in contig CM000154_GR.
KEGG	ddi:DDBDRAFT_0219249.
Organism-specific databases
dictyBase	DDB_G0287659. hexb2.
Phylogenomic databases
OMA	THIYTHE.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...

Entry information

Entry name

HEXB2_DICDI

Accession

Primary (citable) accession number: Q54K56

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)