Reviewed, UniProtKB/Swiss-Prot Q54K55 (HEXB1_DICDI)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit B1
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit B1
    Beta-N-acetylhexosaminidase subunit B1

Gene names

Name:	hexb1
ORF Names:	DDB_G0287597

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 560	535	Beta-hexosaminidase subunit B1		PRO_0000331237
Sites
	Active site	359	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential
	Glycosylation	472	1	N-linked (GlcNAc...) Potential
	Glycosylation	547	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54K55-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: DF3700F79651C682	FASTA	560	64,555
	10 20 30 40 50 60 MIILKRNIVF LLIIIIVLGI FIATSIEIKN YKLSLNQNKN EISKNPPIWP APFYGQFGNN 70 80 90 100 110 120 SILISKEFNF TIISDSTLLL NKTLSKYYNL IFTQDNLINS SSNTLNKLNI NLKSKNEILK 130 140 150 160 170 180 FGFDESYKLI IKNNENSKLE GNTVYGIMRG LETFYQLIKY NFSDNSYFIE NCLPLIINDK 190 200 210 220 230 240 PRFPHRGVML DTSRHFYSVD TILKVIESLS YNKFNTLHWH IIDSQSFPLS SKSYPNLING 250 260 270 280 290 300 AWSKSEIYSY HDIKRIIKYG KENGIRIQLE IDMPGHAKSW SVGYPDLLPH GWNDSTTTIK 310 320 330 340 350 360 CPDYDVPLDP SSPLSLPISF GLLSEFSGTD YGYNPNYDDK SNNLFNLTVD DLFHVGGDEI 370 380 390 400 410 420 EYQCWNNSKR IKDWMNENNL KTFQDVAKQF QLKIIKQLLK IGKIPVLWED TFQLFYKDLP 430 440 450 460 470 480 KDVIVEIYHD QSTAINATNN GYKIISSIAR YWYLEYSYSN WIRAYNFEPT LNISKSNIHL 490 500 510 520 530 540 VLGGEGAIWS ESIDSSNLFQ KLYPTSSAIA ERLWSPIYYT NLLNAKSRLQ SFRCSLLKRG 550 560 INSAPLNNSS PLSAFSCYNS

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000103 Genomic DNA. Translation: EAL63607.1.
RefSeq	XP_637109.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3388700.
GenomeReviews	Gene locus hexb1 in contig CM000154_GR.
KEGG	ddi:DDBDRAFT_0187537.
Organism-specific databases
dictyBase	DDB_G0287597. hexb1.
Phylogenomic databases
OMA	ISSIARY.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...

Entry information

Entry name

HEXB1_DICDI

Accession

Primary (citable) accession number: Q54K55

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)