Reviewed, UniProtKB/Swiss-Prot Q54K50 (PLDY_DICDI)

Names and origin

Protein names

Recommended name:
    Phospholipase D Y
    EC=3.1.4.4
Alternative name(s):
    Phosphatase D3
      Short name=PLD 3

Gene names

Name:	pldY
Synonyms:	pld3
ORF Names:	DDB_G0287649

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes membrane phospholipids, such as PtdCho (phosphatidylcholine), producing the free headgroup and PtdOH (phosphatidic acid; signaling molecule on its own) By similarity.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Inhibited by butan-1-ol.
Developmental stage	Expression is observed in spores, increases about two- to three-fold at 0.5 hour to 1 hour during spore germination, and then rapidly decreases. The mRNA is not detectable in vegetative cells or in early multicellular development on filters, but is present late during development, approximately at the time of sporulation. Ref.2
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.
Sequence caution	The sequence AAA67429.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 438	419	Phospholipase D Y		PRO_0000367473
Regions
	Domain	145 – 172	28	PLD phosphodiesterase 1
	Domain	373 – 399	27	PLD phosphodiesterase 2
	Compositional bias	233 – 247	15	Poly-Asn
Sites
	Active site	150	1	Potential
	Active site	152	1	Potential
	Active site	157	1	Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	394	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54K50-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 8CA94E4D3C1F2631	FASTA	438	49,186
	10 20 30 40 50 60 MIINRLFIII VLFFVNVNSK IISESTLNSC QGGTIQIAES IPLGLDISTN LSTHDAWMDL 70 80 90 100 110 120 ITNAQESIDL GFFYFTLLGG SDLDPVYGGQ LGIDIFNAIV EAHSRGINVR IVQNEPSESF 130 140 150 160 170 180 PDTETQTLAK LGIQVRSIDW VSLVGSGVLH TKLIIIDESS AYVGSANADW SSLAQVKELG 190 200 210 220 230 240 IVLKNCPTMV ADTEIAFQQY WNAADFTSLP INDWGSNYQA LFNNTNMASL SLNNNNNNNN 250 260 270 280 290 300 KNKNNDNDGS GTNQMYEMFL AVSPPQFQST YRTGDIDALM DAINNADQSI CITVMDYTPT 310 320 330 340 350 360 TLYNDPNTYW PLIDNALRAA AFNRNVQVRM LISHWNYTSP IIPQWLHSLN QVDNIQVRWF 370 380 390 400 410 420 VVPDFSTNPQ IPYTRVNHAK FMVTEKQSYV GTSNWSQDYF TVTGGLSYNV FNNDFTNQLQ 430 SIFNRDWNSP YTQPIQNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Organization of a gene family developmentally regulated during Dictyostelium discoideum spore germination." Giorda R., Ohmachi T., Ennis H.L. J. Mol. Biol. 205:63-69(1989) [PubMed: 2926809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-429, DEVELOPMENTAL STAGE. Strain: AX3 / DH1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000103 Genomic DNA. Translation: EAL63633.1. M19469 Genomic DNA. Translation: AAA67429.1. Sequence problems.
RefSeq	XP_637114.1.
3D structure databases
SMR	Q54K50. Positions 54-428.
ModBase	Search...
Genome annotation databases
GeneID	8626209.
KEGG	ddi:DDB_0220113.
Organism-specific databases
dictyBase	DDB_G0287649. pldY.
Phylogenomic databases
eggNOG	KOG3603.
HOGENOM	HBG444645.
InParanoid	Q54K50.
OMA	IDESSAY.
PhylomeDB	Q54K50.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDY_DICDI

Accession

Primary (citable) accession number: Q54K50
Secondary accession number(s): Q23849

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)