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Q54JH6

- CMT1_DICDI

UniProt

Q54JH6 - CMT1_DICDI

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Protein
DNA (cytosine-5)-methyltransferase
Gene
dnmA, DDB_G0288047
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: dictyBase
  2. DNA binding Source: dictyBase
  3. tRNA (cytosine-5-)-methyltransferase activity Source: dictyBase
Complete GO annotation...

GO - Biological processi

  1. C-5 methylation of cytosine Source: GOC
  2. DNA methylation Source: dictyBase
  3. DNA methylation on cytosine Source: dictyBase
  4. chromatin modification Source: UniProtKB-KW
  5. culmination involved in sorocarp development Source: dictyBase
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. sporulation resulting in formation of a cellular spore Source: dictyBase
  8. tRNA methylation Source: GOC
  9. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase (EC:2.1.1.37)
Gene namesi
Name:dnmA
ORF Names:DDB_G0288047
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288047. dnmA.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit altered gene expression, and after undergoing 16 development cycles some mutant cells displayed reduced growth on axenic medium. DIRS-1 expression was unaffected but Skipper expression was increased in mutant cells after 16 cycles of development. Mutants lacking dmaA exhibit culmination defects including fragmented, transluscent sori, and fewer spores than wild type.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379DNA (cytosine-5)-methyltransferase
PRO_0000328901Add
BLAST

Expressioni

Developmental stagei

Expressed during vegetative growth and throughout development. Levels decrease after aggregation and are lowest during culmination.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231095.

Structurei

3D structure databases

ProteinModelPortaliQ54JH6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 366363SAM-dependent MTase C5-type
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi191 – 20818Asn-rich
Add
BLAST
Compositional biasi364 – 3674Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0270.
KOiK15336.
OMAiQSFDINT.
PhylomeDBiQ54JH6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54JH6-1 [UniParc]FASTAAdd to Basket

« Hide

MEQLRVLEFY SGIGGMHYGL QESGVDFQVI QSFDINTNAN LNYKYTFNED    50
SSQKSIESYS VEELEGFKAN AWLMSPPCQP FTRLGLQKDD QDNRTNSFFH 100
LLDVLTKIKD PPTYILIENV FGFAKKGSSN TRDHLLDTLI KMNYSFQEFH 150
LSPQQFGLAN QRLRYFCIAK RNGKLNFKKE QDKHNEKVDE NKLNNNSNNN 200
NEQNKYDNLK ILDHIPGYDF HTTLEECDEI SNYFDKDLTD DELYEKYKVP 250
HNLLLSKGML FDIKQKDSKT SNCVTKSYGK FIEGTGSIIQ MDNNFKADIN 300
DNKSLIPLKL RYFSPKEITR LHGFPEEFKF SPKLTTIQCY RLIGNSLNVK 350
IVSELLKVLV SPNEEEEQQE QQKEKEGKK 379
Length:379
Mass (Da):44,115
Last modified:May 24, 2005 - v1
Checksum:i3C9B2D277BA9D25A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000107 Genomic DNA. Translation: EAL63449.1.
RefSeqiXP_636955.1. XM_631863.1.

Genome annotation databases

EnsemblProtistsiDDB0231095; DDB0231095; DDB_G0288047.
GeneIDi8626430.
KEGGiddi:DDB_G0288047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000107 Genomic DNA. Translation: EAL63449.1 .
RefSeqi XP_636955.1. XM_631863.1.

3D structure databases

ProteinModelPortali Q54JH6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231095.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231095 ; DDB0231095 ; DDB_G0288047 .
GeneIDi 8626430.
KEGGi ddi:DDB_G0288047.

Organism-specific databases

dictyBasei DDB_G0288047. dnmA.

Phylogenomic databases

eggNOGi COG0270.
KOi K15336.
OMAi QSFDINT.
PhylomeDBi Q54JH6.

Miscellaneous databases

PROi Q54JH6.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Evolutionary diversification of DNA methyltransferases in eukaryotic genomes."
    Ponger L., Li W.H.
    Mol. Biol. Evol. 22:1119-1128(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  3. Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  4. "Developmentally regulated DNA methylation in Dictyostelium discoideum."
    Katoh M., Curk T., Xu Q., Zupan B., Kuspa A., Shaulsky G.
    Eukaryot. Cell 5:18-25(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCMT1_DICDI
AccessioniPrimary (citable) accession number: Q54JH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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