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Q54JH6

- CMT1_DICDI

UniProt

Q54JH6 - CMT1_DICDI

Protein

DNA (cytosine-5)-methyltransferase

Gene

dnmA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper.2 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei78 – 781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: dictyBase
    2. DNA binding Source: dictyBase
    3. tRNA (cytosine-5-)-methyltransferase activity Source: dictyBase

    GO - Biological processi

    1. C-5 methylation of cytosine Source: GOC
    2. chromatin modification Source: UniProtKB-KW
    3. culmination involved in sorocarp development Source: dictyBase
    4. DNA methylation Source: dictyBase
    5. DNA methylation on cytosine Source: dictyBase
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. sporulation resulting in formation of a cellular spore Source: dictyBase
    8. transcription, DNA-templated Source: UniProtKB-KW
    9. tRNA methylation Source: GOC

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase (EC:2.1.1.37)
    Gene namesi
    Name:dnmA
    ORF Names:DDB_G0288047
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288047. dnmA.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: dictyBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mutants exhibit altered gene expression, and after undergoing 16 development cycles some mutant cells displayed reduced growth on axenic medium. DIRS-1 expression was unaffected but Skipper expression was increased in mutant cells after 16 cycles of development. Mutants lacking dmaA exhibit culmination defects including fragmented, transluscent sori, and fewer spores than wild type.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379DNA (cytosine-5)-methyltransferasePRO_0000328901Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed during vegetative growth and throughout development. Levels decrease after aggregation and are lowest during culmination.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231095.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54JH6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 366363SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi191 – 20818Asn-richAdd
    BLAST
    Compositional biasi364 – 3674Poly-Glu

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0270.
    KOiK15336.
    OMAiQSFDINT.
    PhylomeDBiQ54JH6.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54JH6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQLRVLEFY SGIGGMHYGL QESGVDFQVI QSFDINTNAN LNYKYTFNED    50
    SSQKSIESYS VEELEGFKAN AWLMSPPCQP FTRLGLQKDD QDNRTNSFFH 100
    LLDVLTKIKD PPTYILIENV FGFAKKGSSN TRDHLLDTLI KMNYSFQEFH 150
    LSPQQFGLAN QRLRYFCIAK RNGKLNFKKE QDKHNEKVDE NKLNNNSNNN 200
    NEQNKYDNLK ILDHIPGYDF HTTLEECDEI SNYFDKDLTD DELYEKYKVP 250
    HNLLLSKGML FDIKQKDSKT SNCVTKSYGK FIEGTGSIIQ MDNNFKADIN 300
    DNKSLIPLKL RYFSPKEITR LHGFPEEFKF SPKLTTIQCY RLIGNSLNVK 350
    IVSELLKVLV SPNEEEEQQE QQKEKEGKK 379
    Length:379
    Mass (Da):44,115
    Last modified:May 24, 2005 - v1
    Checksum:i3C9B2D277BA9D25A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000107 Genomic DNA. Translation: EAL63449.1.
    RefSeqiXP_636955.1. XM_631863.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231095; DDB0231095; DDB_G0288047.
    GeneIDi8626430.
    KEGGiddi:DDB_G0288047.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000107 Genomic DNA. Translation: EAL63449.1 .
    RefSeqi XP_636955.1. XM_631863.1.

    3D structure databases

    ProteinModelPortali Q54JH6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231095.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231095 ; DDB0231095 ; DDB_G0288047 .
    GeneIDi 8626430.
    KEGGi ddi:DDB_G0288047.

    Organism-specific databases

    dictyBasei DDB_G0288047. dnmA.

    Phylogenomic databases

    eggNOGi COG0270.
    KOi K15336.
    OMAi QSFDINT.
    PhylomeDBi Q54JH6.

    Miscellaneous databases

    PROi Q54JH6.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Evolutionary diversification of DNA methyltransferases in eukaryotic genomes."
      Ponger L., Li W.H.
      Mol. Biol. Evol. 22:1119-1128(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    3. Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    4. "Developmentally regulated DNA methylation in Dictyostelium discoideum."
      Katoh M., Curk T., Xu Q., Zupan B., Kuspa A., Shaulsky G.
      Eukaryot. Cell 5:18-25(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCMT1_DICDI
    AccessioniPrimary (citable) accession number: Q54JH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3