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Protein

DNA (cytosine-5)-methyltransferase

Gene

dnmA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: dictyBase
  • DNA binding Source: dictyBase
  • tRNA (cytosine-5-)-methyltransferase activity Source: dictyBase

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • culmination involved in sorocarp development Source: dictyBase
  • DNA methylation Source: dictyBase
  • DNA methylation on cytosine Source: dictyBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase (EC:2.1.1.37)
Gene namesi
Name:dnmA
ORF Names:DDB_G0288047
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288047 dnmA

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: dictyBase

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit altered gene expression, and after undergoing 16 development cycles some mutant cells displayed reduced growth on axenic medium. DIRS-1 expression was unaffected but Skipper expression was increased in mutant cells after 16 cycles of development. Mutants lacking dmaA exhibit culmination defects including fragmented, transluscent sori, and fewer spores than wild type.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289011 – 379DNA (cytosine-5)-methyltransferaseAdd BLAST379

Proteomic databases

PaxDbiQ54JH6

Expressioni

Developmental stagei

Expressed during vegetative growth and throughout development. Levels decrease after aggregation and are lowest during culmination.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231095

Structurei

3D structure databases

ProteinModelPortaliQ54JH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 366SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST363

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 208Asn-richAdd BLAST18
Compositional biasi364 – 367Poly-Glu4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0919 Eukaryota
COG0270 LUCA
InParanoidiQ54JH6
KOiK15336
OMAiLRYFTPK
PhylomeDBiQ54JH6

Family and domain databases

InterProiView protein in InterPro
IPR001525 C5_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

Q54JH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQLRVLEFY SGIGGMHYGL QESGVDFQVI QSFDINTNAN LNYKYTFNED
60 70 80 90 100
SSQKSIESYS VEELEGFKAN AWLMSPPCQP FTRLGLQKDD QDNRTNSFFH
110 120 130 140 150
LLDVLTKIKD PPTYILIENV FGFAKKGSSN TRDHLLDTLI KMNYSFQEFH
160 170 180 190 200
LSPQQFGLAN QRLRYFCIAK RNGKLNFKKE QDKHNEKVDE NKLNNNSNNN
210 220 230 240 250
NEQNKYDNLK ILDHIPGYDF HTTLEECDEI SNYFDKDLTD DELYEKYKVP
260 270 280 290 300
HNLLLSKGML FDIKQKDSKT SNCVTKSYGK FIEGTGSIIQ MDNNFKADIN
310 320 330 340 350
DNKSLIPLKL RYFSPKEITR LHGFPEEFKF SPKLTTIQCY RLIGNSLNVK
360 370
IVSELLKVLV SPNEEEEQQE QQKEKEGKK
Length:379
Mass (Da):44,115
Last modified:May 24, 2005 - v1
Checksum:i3C9B2D277BA9D25A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000107 Genomic DNA Translation: EAL63449.1
RefSeqiXP_636955.1, XM_631863.1

Genome annotation databases

EnsemblProtistsiEAL63449; EAL63449; DDB_G0288047
GeneIDi8626430
KEGGiddi:DDB_G0288047

Similar proteinsi

Entry informationi

Entry nameiCMT1_DICDI
AccessioniPrimary (citable) accession number: Q54JH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 25, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome