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Protein

DNA (cytosine-5)-methyltransferase

Gene

dnmA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: dictyBase
  • DNA binding Source: dictyBase
  • tRNA (cytosine-5-)-methyltransferase activity Source: dictyBase

GO - Biological processi

  • C-5 methylation of cytosine Source: GOC
  • chromatin modification Source: UniProtKB-KW
  • culmination involved in sorocarp development Source: dictyBase
  • DNA methylation Source: dictyBase
  • DNA methylation on cytosine Source: dictyBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA methylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase (EC:2.1.1.37)
Gene namesi
Name:dnmA
ORF Names:DDB_G0288047
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288047. dnmA.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit altered gene expression, and after undergoing 16 development cycles some mutant cells displayed reduced growth on axenic medium. DIRS-1 expression was unaffected but Skipper expression was increased in mutant cells after 16 cycles of development. Mutants lacking dmaA exhibit culmination defects including fragmented, transluscent sori, and fewer spores than wild type.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379DNA (cytosine-5)-methyltransferasePRO_0000328901Add
BLAST

Expressioni

Developmental stagei

Expressed during vegetative growth and throughout development. Levels decrease after aggregation and are lowest during culmination.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231095.

Structurei

3D structure databases

ProteinModelPortaliQ54JH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 366363SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi191 – 20818Asn-richAdd
BLAST
Compositional biasi364 – 3674Poly-Glu

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
InParanoidiQ54JH6.
KOiK15336.
OMAiQSFDINT.
PhylomeDBiQ54JH6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54JH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQLRVLEFY SGIGGMHYGL QESGVDFQVI QSFDINTNAN LNYKYTFNED
60 70 80 90 100
SSQKSIESYS VEELEGFKAN AWLMSPPCQP FTRLGLQKDD QDNRTNSFFH
110 120 130 140 150
LLDVLTKIKD PPTYILIENV FGFAKKGSSN TRDHLLDTLI KMNYSFQEFH
160 170 180 190 200
LSPQQFGLAN QRLRYFCIAK RNGKLNFKKE QDKHNEKVDE NKLNNNSNNN
210 220 230 240 250
NEQNKYDNLK ILDHIPGYDF HTTLEECDEI SNYFDKDLTD DELYEKYKVP
260 270 280 290 300
HNLLLSKGML FDIKQKDSKT SNCVTKSYGK FIEGTGSIIQ MDNNFKADIN
310 320 330 340 350
DNKSLIPLKL RYFSPKEITR LHGFPEEFKF SPKLTTIQCY RLIGNSLNVK
360 370
IVSELLKVLV SPNEEEEQQE QQKEKEGKK
Length:379
Mass (Da):44,115
Last modified:May 24, 2005 - v1
Checksum:i3C9B2D277BA9D25A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000107 Genomic DNA. Translation: EAL63449.1.
RefSeqiXP_636955.1. XM_631863.1.

Genome annotation databases

EnsemblProtistsiDDB0231095; DDB0231095; DDB_G0288047.
GeneIDi8626430.
KEGGiddi:DDB_G0288047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000107 Genomic DNA. Translation: EAL63449.1.
RefSeqiXP_636955.1. XM_631863.1.

3D structure databases

ProteinModelPortaliQ54JH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231095; DDB0231095; DDB_G0288047.
GeneIDi8626430.
KEGGiddi:DDB_G0288047.

Organism-specific databases

dictyBaseiDDB_G0288047. dnmA.

Phylogenomic databases

eggNOGiCOG0270.
InParanoidiQ54JH6.
KOiK15336.
OMAiQSFDINT.
PhylomeDBiQ54JH6.

Miscellaneous databases

PROiQ54JH6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Evolutionary diversification of DNA methyltransferases in eukaryotic genomes."
    Ponger L., Li W.H.
    Mol. Biol. Evol. 22:1119-1128(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  3. Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  4. "Developmentally regulated DNA methylation in Dictyostelium discoideum."
    Katoh M., Curk T., Xu Q., Zupan B., Kuspa A., Shaulsky G.
    Eukaryot. Cell 5:18-25(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCMT1_DICDI
AccessioniPrimary (citable) accession number: Q54JH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.