Reviewed, UniProtKB/Swiss-Prot Q54JH6 (CMT1_DICDI)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase
    EC=2.1.1.37

Gene names

Name:	dnmA
ORF Names:	DDB_G0288047

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in epigenetic gene silencing. Methylates specific cytosine residues in the retrotransposons DIRS-1 and Skipper. Ref.3 Ref.4
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine. Ref.3 Ref.4
Subcellular location	Nucleus By similarity.
Developmental stage	Expressed during vegetative growth and throughout development. Levels decrease after aggregation and are lowest during culmination. Ref.3
Disruption phenotype	Mutants exhibit altered gene expression, and after undergoing 16 development cycles some mutant cells displayed reduced growth on axenic medium. DIRS-1 expression was unaffected but Skipper expression was increased in mutant cells after 16 cycles of development. Mutants lacking dmaA exhibit culmination defects including fragmented, transluscent sori, and fewer spores than wild type. Ref.3 Ref.4
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	DNA (cytosine-5)-methyltransferase		PRO_0000328901
Regions
	Compositional bias	191 – 208	18	Asn-rich
	Compositional bias	364 – 367	4	Poly-Glu
Sites
	Active site	78	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54JH6-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 3C9B2D277BA9D25A	FASTA	379	44,115
	10 20 30 40 50 60 MEQLRVLEFY SGIGGMHYGL QESGVDFQVI QSFDINTNAN LNYKYTFNED SSQKSIESYS 70 80 90 100 110 120 VEELEGFKAN AWLMSPPCQP FTRLGLQKDD QDNRTNSFFH LLDVLTKIKD PPTYILIENV 130 140 150 160 170 180 FGFAKKGSSN TRDHLLDTLI KMNYSFQEFH LSPQQFGLAN QRLRYFCIAK RNGKLNFKKE 190 200 210 220 230 240 QDKHNEKVDE NKLNNNSNNN NEQNKYDNLK ILDHIPGYDF HTTLEECDEI SNYFDKDLTD 250 260 270 280 290 300 DELYEKYKVP HNLLLSKGML FDIKQKDSKT SNCVTKSYGK FIEGTGSIIQ MDNNFKADIN 310 320 330 340 350 360 DNKSLIPLKL RYFSPKEITR LHGFPEEFKF SPKLTTIQCY RLIGNSLNVK IVSELLKVLV 370 SPNEEEEQQE QQKEKEGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Evolutionary diversification of DNA methyltransferases in eukaryotic genomes." Ponger L., Li W.H. Mol. Biol. Evol. 22:1119-1128(2005) [PubMed: 15689527] [Abstract] Cited for: IDENTIFICATION.
[3]	"Silencing of retrotransposons in Dictyostelium by DNA methylation and RNAi." Kuhlmann M., Borisova B.E., Kaller M., Larsson P., Stach D., Na J., Eichinger L., Lyko F., Ambros V., Soderbom F., Hammann C., Nellen W. Nucleic Acids Res. 33:6405-6417(2005) [PubMed: 16282589] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[4]	"Developmentally regulated DNA methylation in Dictyostelium discoideum." Katoh M., Curk T., Xu Q., Zupan B., Kuspa A., Shaulsky G. Eukaryot. Cell 5:18-25(2006) [PubMed: 16400165] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000107 Genomic DNA. Translation: EAL63449.1.
RefSeq	XP_636955.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54JH6.
Genome annotation databases
GeneID	3388526.
GenomeReviews	Gene locus dnmA in contig CM000154_GR.
KEGG	ddi:DDB_0231095.
Organism-specific databases
dictyBase	DDB_G0288047. dnmA.
Phylogenomic databases
OMA	EIANLHG.
Enzyme and pathway databases
BRENDA	2.1.1.37. 424.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
ProtoNet	Search...

Entry information

Entry name

CMT1_DICDI

Accession

Primary (citable) accession number: Q54JH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)