Q54JC8 (PUR4_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Name:	purL
ORF Names:	DDB_G0288145

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1355	1355	Phosphoribosylformylglycinamidine synthase		PRO_0000327671
Regions
	Domain	1087 – 1325	239	Glutamine amidotransferase type-1
	Nucleotide binding	326 – 337	12	ATP Potential
	Compositional bias	81 – 86	6	Poly-Thr
	Compositional bias	992 – 1000	9	Poly-Asn
Sites
	Active site	1182	1	Nucleophile; for GATase activity By similarity
	Active site	1310	1	For GATase activity By similarity
	Active site	1312	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54JC8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 859E9DCBD36E5A6C	FASTA	1,355	149,827
	10 20 30 40 50 60 MTIQQFYRKP AISEYEIKLL KNNLKKQHNI DIESIETEYC FNVQYPDNHK LNESEQSTLV 70 80 90 100 110 120 WLLSETFEPK NFSIDKSFLK TTTTTTENEI IIEVGPRMNF TTTYSSNATS ICKSCNLSII 130 140 150 160 170 180 DRIERSRRYL VKSVSKLSEK QIDQFLELIH DRMTECLYPT PIKSFDTGII PKAVVYIPVV 190 200 210 220 230 240 EEGRAALERV NKEMGLAFDE QDLALYTDLF QNQLKRNPSD VECFDIGQSN SEHSRHWFFN 250 260 270 280 290 300 GKLIVDGNMS DKTLFQIVKN TLKANPQNSL IAFSDNSSSI KGFKTKVLIP KSQIEASEYL 310 320 330 340 350 360 EGEREQPIIF TAETHNFPTG IAPFEGAETG TGGRLRDTHA TGRGSLVVAG TVGYCVGNLN 370 380 390 400 410 420 IPGYELPWEN KEYNYPDNMA NPLKIEIEAS NGASDYGNKF GEPVIIGFTR SYGNTLPNGE 430 440 450 460 470 480 RREWIKPIMF SGGIGFMDER HLKKEQPEIG MVVVKAGGPA YRIGMGGGSA SSMVGGDNKH 490 500 510 520 530 540 ELDFSAVQRG DAEMGQKLNR IVRSCVESEI HGGCNPIVSV HDQGAGGAGN VLKEIVDPLG 550 560 570 580 590 600 AKIYLDRIIS GDPTLSAMEI WGAEYQENDA LLIKAEHKDY LKKVSERERL PIAFVGDVTG 610 620 630 640 650 660 DGIAQLITKD GETPVNLPLD KVLQKMPPKT FVLDHVEKQL KPFTLPKELL VGDHQTCFNE 670 680 690 700 710 720 CLNRVLRLLS VGSKRFLINK VDRAVTGLVA RQQCVGPLHT PVSNVAVISS GYFGKSGAAT 730 740 750 760 770 780 SIGEQPIKGF ISAKSMAYLT VGEALTNLMW ASITDLGDVK CSGNWMWAAK LKGEGVELYD 790 800 810 820 830 840 AAIEMHDVMV ELGIAIDGGK DSLSMAAKAP KSDGSQELVK APGALVVSTY VPCDDITLTV 850 860 870 880 890 900 TPDLKLSSKD DSVILYLDLG CANNFIGGSA LTQVFNQVGN DEPHHNTPLL KNTFMAIQKL 910 920 930 940 950 960 VKQQLISAGH DRSDGGLITT LIEMSLSGNR GLEINLPDTH NSDQSPLSII KLLFSEELGA 970 980 990 1000 1010 1020 VLEIKKSNQQ IVLDILKQFN VPTQVIGNTS CNNNNNNNNN GSDEDLFIVK VGDKLIYNIK 1030 1040 1050 1060 1070 1080 LSQLSKQWEE TSYQLELLQA NPTFVESEMK NLLKRATGKG KGPNYNMTYK ISPISKELAL 1090 1100 1110 1120 1130 1140 LANKAPKVAV IREEGSNGDR EMAAAFHFAG FQAFDVTMSD LLNGNIQLDE RFKGVAFVGG 1150 1160 1170 1180 1190 1200 FSYGDVMDSA KGWAGSIRFN QQVSKQFDHF YGRNDTFSLG LCNGCQLMAL LGWVPYRGIE 1210 1220 1230 1240 1250 1260 QTHQPRFIHN ASGRFESRWV NVKIMPSPAL LLKGMEGSVL GVWSQHGEGR FWSEDQSIVN 1270 1280 1290 1300 1310 1320 DIKANNLSPI RYVDDDGEIT ESYPFNPSGT QEGFASLCSK DGRHLAIMPH PERSFLSWQW 1330 1340 1350 PFMPENIKQN VGGLDQPSPW IKIFQNAKSF CDSLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Veltman D.M., Insall R.H. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 164-184 AND 534-542, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000109 Genomic DNA. Translation: EAL63360.1.
RefSeq	XP_636865.1. XM_631773.1.
3D structure databases
HSSP	HSSP built from PDB template 1T3T based on UniProtKB P74881.
ProteinModelPortal	Q54JC8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54JC8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0230086; DDB0230086; DDB_G0288145.
GeneID	8626476.
GenomeReviews	Gene locus purL in contig CM000154_GR.
KEGG	ddi:DDB_G0288145.
Organism-specific databases
dictyBase	DDB_G0288145. purL.
Phylogenomic databases
eggNOG	KOG1907.
GeneTree	EPrGT00050000006085.
HOGENOM	HBG335309.
OMA	VKAVGME.
PhylomeDB	Q54JC8.
ProtClustDB	CLSZ2429920.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_DICDI

Accession

Primary (citable) accession number: Q54JC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)