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Q54J73

- PAP_DICDI

UniProt

Q54J73 - PAP_DICDI

Protein

Poly(A) polymerase

Gene

papA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi146 – 1461Magnesium 1; catalyticBy similarity
    Metal bindingi146 – 1461Magnesium 2; catalyticBy similarity
    Metal bindingi148 – 1481Magnesium 1; catalyticBy similarity
    Metal bindingi148 – 1481Magnesium 2; catalyticBy similarity
    Metal bindingi200 – 2001Magnesium 2; catalyticBy similarity
    Binding sitei200 – 2001ATPBy similarity
    Binding sitei262 – 2621ATPBy similarity
    Binding sitei271 – 2711ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi133 – 1353ATPBy similarity
    Nucleotide bindingi146 – 1483ATPBy similarity
    Nucleotide bindingi280 – 2812ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: dictyBase
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA polyadenylation Source: dictyBase

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase (EC:2.7.7.19)
    Short name:
    PAP
    Alternative name(s):
    Polynucleotide adenylyltransferase
    Gene namesi
    Name:papA
    ORF Names:DDB_G0288259
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288259. papA.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. mRNA cleavage and polyadenylation specificity factor complex Source: dictyBase
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 809809Poly(A) polymerasePRO_0000330665Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0216279.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54J73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi17 – 259Poly-Thr
    Compositional biasi575 – 776202Thr-richAdd
    BLAST
    Compositional biasi786 – 7894Poly-Ser

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiCOG5186.
    KOiK14376.
    OMAiISEDANC.
    PhylomeDBiQ54J73.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54J73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP    50
    TILGVTEPIS TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK 100
    LNQIVREWAK QVSLKKGYPE QTASEVVAKI FTFGSYRLGV HGPGSDIDTL 150
    CVGPKHIMRS DFFDDLSDIL KVHPEITEFT TVKDAFVPVI TMVFSGIPID 200
    LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV TDQILKLVPN 250
    IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS 300
    TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP 350
    AYPSMNSTYN VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF 400
    TRYSFYIEID CYSMNEEDSR KWEGWIESKL RFLISNLEST PKMKFAVPYP 450
    KGFTNNLHKA NNPDQICTSF FMGLSFNFSN TPGADKSVDL TKAVTEFTGI 500
    IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK TTKKRSSTGE 550
    PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA 600
    TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT 650
    TTINSVQPPS AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI 700
    TLPTTLESQT STLKDSNEIS TNGTAVATEP TITSPSVNIN ESSTSTSTTT 750
    TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN ELSFISSSSE TSQSKPPPKK 800
    PTISIIRGN 809
    Length:809
    Mass (Da):88,686
    Last modified:May 24, 2005 - v1
    Checksum:i382E40C4D6A94D78
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000109 Genomic DNA. Translation: EAL63311.1.
    RefSeqiXP_636814.1. XM_631722.1.

    Genome annotation databases

    EnsemblProtistsiDDB0216279; DDB0216279; DDB_G0288259.
    GeneIDi8626531.
    KEGGiddi:DDB_G0288259.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000109 Genomic DNA. Translation: EAL63311.1 .
    RefSeqi XP_636814.1. XM_631722.1.

    3D structure databases

    ProteinModelPortali Q54J73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0216279.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0216279 ; DDB0216279 ; DDB_G0288259 .
    GeneIDi 8626531.
    KEGGi ddi:DDB_G0288259.

    Organism-specific databases

    dictyBasei DDB_G0288259. papA.

    Phylogenomic databases

    eggNOGi COG5186.
    KOi K14376.
    OMAi ISEDANC.
    PhylomeDBi Q54J73.

    Miscellaneous databases

    PROi Q54J73.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPAP_DICDI
    AccessioniPrimary (citable) accession number: Q54J73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3