Q54J73 (PAP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase
Short name=PAP
EC=2.7.7.19

Alternative name(s):
Polynucleotide adenylyltransferase

Gene names

Name:	papA
ORF Names:	DDB_G0288259

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Binds 2 magnesium ions. Also active with manganese By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the poly(A) polymerase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	mRNA cleavage and polyadenylation specificity factor complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Poly(A) polymerase		PRO_0000330665
Regions
	Nucleotide binding	133 – 135	3	ATP By similarity
	Nucleotide binding	146 – 148	3	ATP By similarity
	Nucleotide binding	280 – 281	2	ATP By similarity
	Compositional bias	17 – 25	9	Poly-Thr
	Compositional bias	575 – 776	202	Thr-rich
	Compositional bias	786 – 789	4	Poly-Ser
Sites
	Metal binding	146	1	Magnesium 1; catalytic By similarity
	Metal binding	146	1	Magnesium 2; catalytic By similarity
	Metal binding	148	1	Magnesium 1; catalytic By similarity
	Metal binding	148	1	Magnesium 2; catalytic By similarity
	Metal binding	200	1	Magnesium 2; catalytic By similarity
	Binding site	200	1	ATP By similarity
	Binding site	262	1	ATP By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54J73 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 382E40C4D6A94D78	FASTA	809	88,686
	10 20 30 40 50 60 MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP TILGVTEPIS 70 80 90 100 110 120 TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK LNQIVREWAK QVSLKKGYPE 130 140 150 160 170 180 QTASEVVAKI FTFGSYRLGV HGPGSDIDTL CVGPKHIMRS DFFDDLSDIL KVHPEITEFT 190 200 210 220 230 240 TVKDAFVPVI TMVFSGIPID LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV 250 260 270 280 290 300 TDQILKLVPN IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS 310 320 330 340 350 360 TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP AYPSMNSTYN 370 380 390 400 410 420 VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF TRYSFYIEID CYSMNEEDSR 430 440 450 460 470 480 KWEGWIESKL RFLISNLEST PKMKFAVPYP KGFTNNLHKA NNPDQICTSF FMGLSFNFSN 490 500 510 520 530 540 TPGADKSVDL TKAVTEFTGI IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK 550 560 570 580 590 600 TTKKRSSTGE PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA 610 620 630 640 650 660 TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT TTINSVQPPS 670 680 690 700 710 720 AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI TLPTTLESQT STLKDSNEIS 730 740 750 760 770 780 TNGTAVATEP TITSPSVNIN ESSTSTSTTT TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN 790 800 ELSFISSSSE TSQSKPPPKK PTISIIRGN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000109 Genomic DNA. Translation: EAL63311.1.
RefSeq	XP_636814.1. XM_631722.1.
3D structure databases
ProteinModelPortal	Q54J73.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0216279.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0216279; DDB0216279; DDB_G0288259.
GeneID	8626531.
KEGG	ddi:DDB_G0288259.
Organism-specific databases
dictyBase	DDB_G0288259. papA.
Phylogenomic databases
eggNOG	COG5186.
KO	K14376.
OMA	NINESST.
ProtClustDB	PTZ00418.
Family and domain databases
Gene3D	3.30.70.590. 1 hit.
InterPro	IPR002934. Nucleotidyltransferase. IPR011068. NuclTrfase_I_C. IPR007012. PolA_pol_cen_dom. IPR007010. PolA_pol_RNA-bd_dom. IPR014492. PolyA_polymerase. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF04928. PAP_central. 1 hit. PF04926. PAP_RNA-bind. 1 hit. [Graphical view]
PIRSF	PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAM	SSF55003. PAP_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP_DICDI

Accession

Primary (citable) accession number: Q54J73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)