Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54J73

- PAP_DICDI

UniProt

Q54J73 - PAP_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Poly(A) polymerase

Gene

papA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Magnesium 1; catalyticBy similarity
Metal bindingi146 – 1461Magnesium 2; catalyticBy similarity
Metal bindingi148 – 1481Magnesium 1; catalyticBy similarity
Metal bindingi148 – 1481Magnesium 2; catalyticBy similarity
Metal bindingi200 – 2001Magnesium 2; catalyticBy similarity
Binding sitei200 – 2001ATPBy similarity
Binding sitei262 – 2621ATPBy similarity
Binding sitei271 – 2711ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1353ATPBy similarity
Nucleotide bindingi146 – 1483ATPBy similarity
Nucleotide bindingi280 – 2812ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: dictyBase
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
Polynucleotide adenylyltransferase
Gene namesi
Name:papA
ORF Names:DDB_G0288259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288259. papA.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. mRNA cleavage and polyadenylation specificity factor complex Source: dictyBase
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Poly(A) polymerasePRO_0000330665Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0216279.

Structurei

3D structure databases

ProteinModelPortaliQ54J73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 259Poly-Thr
Compositional biasi575 – 776202Thr-richAdd
BLAST
Compositional biasi786 – 7894Poly-Ser

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
InParanoidiQ54J73.
KOiK14376.
OMAiISEDANC.
PhylomeDBiQ54J73.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54J73-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP
60 70 80 90 100
TILGVTEPIS TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK
110 120 130 140 150
LNQIVREWAK QVSLKKGYPE QTASEVVAKI FTFGSYRLGV HGPGSDIDTL
160 170 180 190 200
CVGPKHIMRS DFFDDLSDIL KVHPEITEFT TVKDAFVPVI TMVFSGIPID
210 220 230 240 250
LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV TDQILKLVPN
260 270 280 290 300
IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS
310 320 330 340 350
TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP
360 370 380 390 400
AYPSMNSTYN VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF
410 420 430 440 450
TRYSFYIEID CYSMNEEDSR KWEGWIESKL RFLISNLEST PKMKFAVPYP
460 470 480 490 500
KGFTNNLHKA NNPDQICTSF FMGLSFNFSN TPGADKSVDL TKAVTEFTGI
510 520 530 540 550
IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK TTKKRSSTGE
560 570 580 590 600
PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA
610 620 630 640 650
TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT
660 670 680 690 700
TTINSVQPPS AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI
710 720 730 740 750
TLPTTLESQT STLKDSNEIS TNGTAVATEP TITSPSVNIN ESSTSTSTTT
760 770 780 790 800
TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN ELSFISSSSE TSQSKPPPKK

PTISIIRGN
Length:809
Mass (Da):88,686
Last modified:May 24, 2005 - v1
Checksum:i382E40C4D6A94D78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63311.1.
RefSeqiXP_636814.1. XM_631722.1.

Genome annotation databases

EnsemblProtistsiDDB0216279; DDB0216279; DDB_G0288259.
GeneIDi8626531.
KEGGiddi:DDB_G0288259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63311.1 .
RefSeqi XP_636814.1. XM_631722.1.

3D structure databases

ProteinModelPortali Q54J73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0216279.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0216279 ; DDB0216279 ; DDB_G0288259 .
GeneIDi 8626531.
KEGGi ddi:DDB_G0288259.

Organism-specific databases

dictyBasei DDB_G0288259. papA.

Phylogenomic databases

eggNOGi COG5186.
InParanoidi Q54J73.
KOi K14376.
OMAi ISEDANC.
PhylomeDBi Q54J73.

Miscellaneous databases

PROi Q54J73.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPAP_DICDI
AccessioniPrimary (citable) accession number: Q54J73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3