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Q54J73

- PAP_DICDI

UniProt

Q54J73 - PAP_DICDI

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Protein

Poly(A) polymerase

Gene
papA, DDB_G0288259
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Magnesium 1; catalytic By similarity
Metal bindingi146 – 1461Magnesium 2; catalytic By similarity
Metal bindingi148 – 1481Magnesium 1; catalytic By similarity
Metal bindingi148 – 1481Magnesium 2; catalytic By similarity
Metal bindingi200 – 2001Magnesium 2; catalytic By similarity
Binding sitei200 – 2001ATP By similarity
Binding sitei262 – 2621ATP By similarity
Binding sitei271 – 2711ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1353ATP By similarity
Nucleotide bindingi146 – 1483ATP By similarity
Nucleotide bindingi280 – 2812ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: dictyBase
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
Polynucleotide adenylyltransferase
Gene namesi
Name:papA
ORF Names:DDB_G0288259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288259. papA.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. mRNA cleavage and polyadenylation specificity factor complex Source: dictyBase
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 809809Poly(A) polymerasePRO_0000330665Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0216279.

Structurei

3D structure databases

ProteinModelPortaliQ54J73.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 259Poly-Thr
Compositional biasi575 – 776202Thr-richAdd
BLAST
Compositional biasi786 – 7894Poly-Ser

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5186.
KOiK14376.
OMAiISEDANC.
PhylomeDBiQ54J73.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54J73-1 [UniParc]FASTAAdd to Basket

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MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP    50
TILGVTEPIS TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK 100
LNQIVREWAK QVSLKKGYPE QTASEVVAKI FTFGSYRLGV HGPGSDIDTL 150
CVGPKHIMRS DFFDDLSDIL KVHPEITEFT TVKDAFVPVI TMVFSGIPID 200
LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV TDQILKLVPN 250
IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS 300
TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP 350
AYPSMNSTYN VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF 400
TRYSFYIEID CYSMNEEDSR KWEGWIESKL RFLISNLEST PKMKFAVPYP 450
KGFTNNLHKA NNPDQICTSF FMGLSFNFSN TPGADKSVDL TKAVTEFTGI 500
IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK TTKKRSSTGE 550
PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA 600
TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT 650
TTINSVQPPS AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI 700
TLPTTLESQT STLKDSNEIS TNGTAVATEP TITSPSVNIN ESSTSTSTTT 750
TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN ELSFISSSSE TSQSKPPPKK 800
PTISIIRGN 809
Length:809
Mass (Da):88,686
Last modified:May 24, 2005 - v1
Checksum:i382E40C4D6A94D78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63311.1.
RefSeqiXP_636814.1. XM_631722.1.

Genome annotation databases

EnsemblProtistsiDDB0216279; DDB0216279; DDB_G0288259.
GeneIDi8626531.
KEGGiddi:DDB_G0288259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63311.1 .
RefSeqi XP_636814.1. XM_631722.1.

3D structure databases

ProteinModelPortali Q54J73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0216279.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0216279 ; DDB0216279 ; DDB_G0288259 .
GeneIDi 8626531.
KEGGi ddi:DDB_G0288259.

Organism-specific databases

dictyBasei DDB_G0288259. papA.

Phylogenomic databases

eggNOGi COG5186.
KOi K14376.
OMAi ISEDANC.
PhylomeDBi Q54J73.

Miscellaneous databases

PROi Q54J73.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPAP_DICDI
AccessioniPrimary (citable) accession number: Q54J73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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