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Protein

Poly(A) polymerase

Gene

papA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Magnesium 1; catalyticBy similarity1
Metal bindingi146Magnesium 2; catalyticBy similarity1
Metal bindingi148Magnesium 1; catalyticBy similarity1
Metal bindingi148Magnesium 2; catalyticBy similarity1
Metal bindingi200Magnesium 2; catalyticBy similarity1
Binding sitei200ATPBy similarity1
Binding sitei262ATPBy similarity1
Binding sitei271ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 135ATPBy similarity3
Nucleotide bindingi146 – 148ATPBy similarity3
Nucleotide bindingi280 – 281ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
Polynucleotide adenylyltransferase
Gene namesi
Name:papA
ORF Names:DDB_G0288259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288259. papA.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003306651 – 809Poly(A) polymeraseAdd BLAST809

Proteomic databases

PaxDbiQ54J73.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0216279.

Structurei

3D structure databases

ProteinModelPortaliQ54J73.
SMRiQ54J73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 25Poly-Thr9
Compositional biasi575 – 776Thr-richAdd BLAST202
Compositional biasi786 – 789Poly-Ser4

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
InParanoidiQ54J73.
KOiK14376.
OMAiMHLRRKE.
PhylomeDBiQ54J73.

Family and domain databases

InterProiView protein in InterPro
IPR011068. NuclTrfase_I-like_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiView protein in Pfam
PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 2 hits.
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54J73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP
60 70 80 90 100
TILGVTEPIS TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK
110 120 130 140 150
LNQIVREWAK QVSLKKGYPE QTASEVVAKI FTFGSYRLGV HGPGSDIDTL
160 170 180 190 200
CVGPKHIMRS DFFDDLSDIL KVHPEITEFT TVKDAFVPVI TMVFSGIPID
210 220 230 240 250
LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV TDQILKLVPN
260 270 280 290 300
IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS
310 320 330 340 350
TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP
360 370 380 390 400
AYPSMNSTYN VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF
410 420 430 440 450
TRYSFYIEID CYSMNEEDSR KWEGWIESKL RFLISNLEST PKMKFAVPYP
460 470 480 490 500
KGFTNNLHKA NNPDQICTSF FMGLSFNFSN TPGADKSVDL TKAVTEFTGI
510 520 530 540 550
IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK TTKKRSSTGE
560 570 580 590 600
PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA
610 620 630 640 650
TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT
660 670 680 690 700
TTINSVQPPS AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI
710 720 730 740 750
TLPTTLESQT STLKDSNEIS TNGTAVATEP TITSPSVNIN ESSTSTSTTT
760 770 780 790 800
TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN ELSFISSSSE TSQSKPPPKK

PTISIIRGN
Length:809
Mass (Da):88,686
Last modified:May 24, 2005 - v1
Checksum:i382E40C4D6A94D78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63311.1.
RefSeqiXP_636814.1. XM_631722.1.

Genome annotation databases

EnsemblProtistsiEAL63311; EAL63311; DDB_G0288259.
GeneIDi8626531.
KEGGiddi:DDB_G0288259.

Similar proteinsi

Entry informationi

Entry nameiPAP_DICDI
AccessioniPrimary (citable) accession number: Q54J73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: November 22, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families