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Q54J73 (PAP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Poly(A) polymerase

Short name=PAP
EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase
Gene names
Name:papA
ORF Names:DDB_G0288259
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Poly(A) polymerase
PRO_0000330665

Regions

Nucleotide binding133 – 1353ATP By similarity
Nucleotide binding146 – 1483ATP By similarity
Nucleotide binding280 – 2812ATP By similarity
Compositional bias17 – 259Poly-Thr
Compositional bias575 – 776202Thr-rich
Compositional bias786 – 7894Poly-Ser

Sites

Metal binding1461Magnesium 1; catalytic By similarity
Metal binding1461Magnesium 2; catalytic By similarity
Metal binding1481Magnesium 1; catalytic By similarity
Metal binding1481Magnesium 2; catalytic By similarity
Metal binding2001Magnesium 2; catalytic By similarity
Binding site2001ATP By similarity
Binding site2621ATP By similarity
Binding site2711ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54J73 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 382E40C4D6A94D78

FASTA80988,686
        10         20         30         40         50         60 
MNKNGGPPVA NITTSSTTIT STTTTQAKSQ LPSSLSVNNL HTTQGSTDQP TILGVTEPIS 

        70         80         90        100        110        120 
TAPPSSIDFK LSTELENTLI SFNLFESPEE SRKREEILGK LNQIVREWAK QVSLKKGYPE 

       130        140        150        160        170        180 
QTASEVVAKI FTFGSYRLGV HGPGSDIDTL CVGPKHIMRS DFFDDLSDIL KVHPEITEFT 

       190        200        210        220        230        240 
TVKDAFVPVI TMVFSGIPID LIYAKLALTA IPEELNDLID ESFLKNIDEK SILSLNGCRV 

       250        260        270        280        290        300 
TDQILKLVPN IPNFRMALRC IKLWAIRRGI YSNILGFLGG VSWALLTARI CQLYPNSAPS 

       310        320        330        340        350        360 
TIIHRFFKVY EIWKWPAPIL LCHIQEGGIL GPKVWNPKRD KAHLMPIITP AYPSMNSTYN 

       370        380        390        400        410        420 
VSKSTLQLMK SEFVRGAEIT RKIETGECTW KNLLEKCDFF TRYSFYIEID CYSMNEEDSR 

       430        440        450        460        470        480 
KWEGWIESKL RFLISNLEST PKMKFAVPYP KGFTNNLHKA NNPDQICTSF FMGLSFNFSN 

       490        500        510        520        530        540 
TPGADKSVDL TKAVTEFTGI IKDWLRTQPN PDTMDIKVQY IKKKQLPAFV KDEGPEEPVK 

       550        560        570        580        590        600 
TTKKRSSTGE PSATRKKLKS ENSDNKLNSP KSPITTNINS TPTTSTPTTT ANTTTNTTTA 

       610        620        630        640        650        660 
TTTTTTTTVP ITSTPTSNIS SPTMNSTELT TPTSTSTTTS NDSITTPPTT TTINSVQPPS 

       670        680        690        700        710        720 
AQPTENGSST SNSPTSTSIN NTALPPNPTT NSESTIETTI TLPTTLESQT STLKDSNEIS 

       730        740        750        760        770        780 
TNGTAVATEP TITSPSVNIN ESSTSTSTTT TTTVTEQQIQ TAPTTATPIN KTIVNTMEVN 

       790        800 
ELSFISSSSE TSQSKPPPKK PTISIIRGN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000109 Genomic DNA. Translation: EAL63311.1.

3D structure databases

ProteinModelPortalQ54J73.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0216279.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0216279; DDB0216279; DDB_G0288259.
KEGGddi:DDB_G0288259.

Organism-specific databases

dictyBaseDDB_G0288259. papA.

Phylogenomic databases

eggNOGCOG5186.
KOK14376.
OMAISEDANC.
PhylomeDBQ54J73.
ProtClustDBPTZ00418.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

PROQ54J73.

Entry information

Entry namePAP_DICDI
AccessionPrimary (citable) accession number: Q54J73
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase