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Protein

Probable pyridoxal 5'-phosphate synthase subunit pdx2

Gene

pdx2

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of pdx1.By similarity

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.By similarity
L-glutamine + H2O = L-glutamate + NH3.

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106NucleophileBy similarity1
Binding sitei136L-glutamineBy similarity1
Active sitei221Charge relay systemBy similarity1
Active sitei223Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Protein family/group databases

MEROPSiC26.A35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridoxal 5'-phosphate synthase subunit pdx2 (EC:4.3.3.6By similarity)
Alternative name(s):
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2By similarity)
Gene namesi
Name:pdx2Curated
ORF Names:DDB_G0288305Imported
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288305. pdx2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • glutaminase complex Source: GO_Central
  • phagocytic vesicle Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004317801 – 248Probable pyridoxal 5'-phosphate synthase subunit pdx2Add BLAST248

Proteomic databases

PaxDbiQ54J48.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0234061.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72L-glutamine bindingBy similarity3
Regioni174 – 175L-glutamine bindingBy similarity2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG3210. Eukaryota.
COG0311. LUCA.
InParanoidiQ54J48.
KOiK08681.
OMAiVFIRAPI.
PhylomeDBiQ54J48.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54J48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELIEENNI KIGVLALQGG FKEHVEMVKS IKHCFSECEN KYKYSIIVQE
60 70 80 90 100
VKSVSDIKKL NPHGIILPGG ESTSMAIIAS SNNDGENIFT FLKEYIKQGN
110 120 130 140 150
FIWGTCAGSI MLSNNVDGQK VGGQSLIGGL DVLISRNYFG RQIDSFETKI
160 170 180 190 200
NLNLKFSKNN NNSILLENFE AIFIRAPAIL DVIDKENVEI IGEYIVTKKD
210 220 230 240
GTKEKVITAV KQNNIIASVF HPELTNDNRF HQYFVQLVLN NHLIKIKI
Length:248
Mass (Da):27,864
Last modified:May 24, 2005 - v1
Checksum:i85975AB38AD9729D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000109 Genomic DNA. Translation: EAL63295.1.
RefSeqiXP_636799.1. XM_631707.1.

Genome annotation databases

EnsemblProtistsiEAL63295; EAL63295; DDB_G0288305.
GeneIDi8626556.
KEGGiddi:DDB_G0288305.

Similar proteinsi

Entry informationi

Entry nameiPDX2_DICDI
AccessioniPrimary (citable) accession number: Q54J48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: May 24, 2005
Last modified: July 5, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families