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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

alg5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-DDI-480985. Synthesis of dolichyl-phosphate-glucose.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5 homolog
Gene namesi
Name:alg5
Synonyms:dgtA
ORF Names:DDB_G0288321
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288321. dgtA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini37 – 327291LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Dolichyl-phosphate beta-glucosyltransferasePRO_0000327383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54J42.
PRIDEiQ54J42.

Expressioni

Inductioni

Regulated by srfA.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191396.

Structurei

3D structure databases

ProteinModelPortaliQ54J42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2977. Eukaryota.
COG0463. LUCA.
InParanoidiQ54J42.
KOiK00729.
OMAiFIRLRYM.
PhylomeDBiQ54J42.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54J42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLFINIAS FTIYGIPVIP LFIIVFVILS YYLLLLHDES PLWLEKENVY
60 70 80 90 100
IDVKEGEQHQ FPSLVENKNP IDNIYLSVIV PAYNEQIRLP SMLDDAIKFL
110 120 130 140 150
NEKSKKDLKF SYEIIIIDDG SKDSTAKLVT SYIEKQPSSN IRLLKLKQNR
160 170 180 190 200
GKGGAVKRGI LCSRGKYCLM VDADGATEFK DFNRVEDIMH KIEKNDLGIV
210 220 230 240 250
CGSRSHLVDS DLVAKRSFLR NILMYGFHIF VQTLCVKGIK DTQCGFKLFT
260 270 280 290 300
RETARRIFPT LHIERWAFDV EILYLAQKLN IPIAEVAVNW TEIDGSKLDP
310 320
FSSSIQMAKD IVRIRFRYLL GIWKIKS
Length:327
Mass (Da):37,636
Last modified:May 24, 2005 - v1
Checksum:i3C071909E78A8527
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63257.1.
AY392442 Genomic DNA. Translation: AAQ98885.1.
RefSeqiXP_636759.1. XM_631667.1.

Genome annotation databases

EnsemblProtistsiDDB0191396; DDB0191396; DDB_G0288321.
GeneIDi8626562.
KEGGiddi:DDB_G0288321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63257.1.
AY392442 Genomic DNA. Translation: AAQ98885.1.
RefSeqiXP_636759.1. XM_631667.1.

3D structure databases

ProteinModelPortaliQ54J42.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191396.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

PaxDbiQ54J42.
PRIDEiQ54J42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191396; DDB0191396; DDB_G0288321.
GeneIDi8626562.
KEGGiddi:DDB_G0288321.

Organism-specific databases

dictyBaseiDDB_G0288321. dgtA.

Phylogenomic databases

eggNOGiKOG2977. Eukaryota.
COG0463. LUCA.
InParanoidiQ54J42.
KOiK00729.
OMAiFIRLRYM.
PhylomeDBiQ54J42.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-DDI-480985. Synthesis of dolichyl-phosphate-glucose.

Miscellaneous databases

PROiQ54J42.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALG5_DICDI
AccessioniPrimary (citable) accession number: Q54J42
Secondary accession number(s): Q6TMJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 6, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.